BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0434.0032.p (258 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KEH9.1 B3KEH9_9BACI Short-chain dehydrogenase/reductase SDR 316 1e-84 B1D7A9.1 B1D7A9_9BACL Short-chain dehydrogenase/reductase SDR 314 6e-84 C2WBS2.1 C2WBS2_BACCE Uncharacterized oxidoreductase yqjQ 267 8e-70 C3BPP4.1 C3BPP4_9BACI Uncharacterized oxidoreductase yqjQ 264 5e-69 C3B7M8.1 C3B7M8_BACMY Uncharacterized oxidoreductase yqjQ 264 6e-69 >B3KEH9.1 B3KEH9_9BACI Short-chain dehydrogenase/reductase SDR Length = 285 Score = 316 bits (809), Expect = 1e-84, Method: Compositional matrix adjust. Identities = 152/256 (59%), Positives = 192/256 (75%) Query: 3 LQDKVVVITGASSGIGALTAQMLGKRGAIPILLARSEDKLKDTAAGIPGVFGLYACDVTD 62 L KVVV+TGASSGIGAL A+ L +GA +L ARS D+L++ A + G L DV + Sbjct: 30 LNHKVVVVTGASSGIGALIAEKLSAQGAYVVLCARSADRLQEVGARLSGPHELATMDVQN 89 Query: 63 EEQVQNTFADILRVYGRIDVLLNNAGYGKFAAFTDMESREFDDMMDVNYMGIVRCTKAVV 122 +QV + + +GRIDVL+NNAGYGKF + DM EF DMMDVNYMGIVRCT+AV+ Sbjct: 90 SDQVSSVMEAVFHRHGRIDVLINNAGYGKFESIMDMPQEEFQDMMDVNYMGIVRCTQAVL 149 Query: 123 PHMLERGSGQIVNVASMAGKIGTARSVAYTATKHAVLGFTNALRQELRRQGIIVSAVNPG 182 P ML++G GQIVN+ASMAGKIGTA+S AY+ATKHAVLGF+N+LRQELR GI VS VNPG Sbjct: 150 PGMLKQGDGQIVNIASMAGKIGTAKSTAYSATKHAVLGFSNSLRQELRNTGITVSTVNPG 209 Query: 183 PIATEFFRTADPSGSYEKSVARMMMTPEHVSKKIVKLIDKGKEEIDLPGFAAGGIRLYGL 242 PI T FF ADP+G Y K+V+ MM ++V+ ++VKL+ K KEE++LP A+ GIRLY L Sbjct: 210 PIDTPFFSLADPTGGYVKNVSWFMMKADYVADQVVKLVHKRKEELNLPRLASFGIRLYQL 269 Query: 243 FPRLADKLTYNLMNRK 258 FPR AD+++Y MN+K Sbjct: 270 FPRFADRVSYKWMNKK 285 >B1D7A9.1 B1D7A9_9BACL Short-chain dehydrogenase/reductase SDR Length = 257 Score = 314 bits (804), Expect = 6e-84, Method: Compositional matrix adjust. Identities = 150/256 (58%), Positives = 196/256 (76%) Query: 3 LQDKVVVITGASSGIGALTAQMLGKRGAIPILLARSEDKLKDTAAGIPGVFGLYACDVTD 62 L+ K+V+I+GASSGIGA+TA+ L K GA+PIL R+ ++L+ AA I +Y DVTD Sbjct: 2 LKGKIVLISGASSGIGAVTAKQLAKLGAVPILTGRNLERLRKHAAAIGAACPMYQMDVTD 61 Query: 63 EEQVQNTFADILRVYGRIDVLLNNAGYGKFAAFTDMESREFDDMMDVNYMGIVRCTKAVV 122 +QVQ T I+ YG+ID+LLNNAG+GKF F + + F+ MMD NYMGIVRCTKAV+ Sbjct: 62 MKQVQQTVDVIIASYGKIDILLNNAGFGKFETFLEADVESFEQMMDTNYMGIVRCTKAVL 121 Query: 123 PHMLERGSGQIVNVASMAGKIGTARSVAYTATKHAVLGFTNALRQELRRQGIIVSAVNPG 182 P+ML++GSG IVN+ASMAGKIG+++S Y+ATKHAVLGFTN+LR EL GI+VSAVNPG Sbjct: 122 PYMLKQGSGHIVNIASMAGKIGSSKSTGYSATKHAVLGFTNSLRMELNGTGIMVSAVNPG 181 Query: 183 PIATEFFRTADPSGSYEKSVARMMMTPEHVSKKIVKLIDKGKEEIDLPGFAAGGIRLYGL 242 PI TEFF ADPSG+Y K+V MM PE+V+ +I+K++++ K E+DLP AA GI+LY L Sbjct: 182 PIRTEFFDVADPSGTYVKNVNWFMMKPEYVASQIIKVMERRKAEVDLPKTAAVGIKLYQL 241 Query: 243 FPRLADKLTYNLMNRK 258 FPR+AD++ NRK Sbjct: 242 FPRIADRVFGGWFNRK 257 >C2WBS2.1 C2WBS2_BACCE Uncharacterized oxidoreductase yqjQ Length = 267 Score = 267 bits (682), Expect = 8e-70, Method: Compositional matrix adjust. Identities = 130/262 (49%), Positives = 182/262 (69%), Gaps = 8/262 (3%) Query: 3 LQDKVVVITGASSGIGALTAQMLGKRGAIPILLARSEDKLK------DTAAGIPGVFGLY 56 LQ+KV+VITGASSGIG A + +GA+P+L+AR+E+KLK T IP + Y Sbjct: 8 LQEKVIVITGASSGIGEQVAMQVAAQGAVPVLIARTEEKLKRLTEKMKTTYNIPCYY--Y 65 Query: 57 ACDVTDEEQVQNTFADILRVYGRIDVLLNNAGYGKFAAFTDMESREFDDMMDVNYMGIVR 116 DV+ E +V+ FA IL+ G+ID+L+NNAG+G F F + E +M +VN G+V Sbjct: 66 VLDVSKESEVETVFARILQDIGQIDILVNNAGFGIFKTFEEASMTEVKEMFEVNVFGLVA 125 Query: 117 CTKAVVPHMLERGSGQIVNVASMAGKIGTARSVAYTATKHAVLGFTNALRQELRRQGIIV 176 CTKAV+PHMLER SGQIVNVAS+AGKI T +S AY A+KHAVLGFTN+LR EL I V Sbjct: 126 CTKAVLPHMLERNSGQIVNVASLAGKIATPKSSAYAASKHAVLGFTNSLRMELSNTNIYV 185 Query: 177 SAVNPGPIATEFFRTADPSGSYEKSVARMMMTPEHVSKKIVKLIDKGKEEIDLPGFAAGG 236 +A+NPGPI T FF AD SG+Y K++ R M+ P +V+++IVK++ K E++LP + + G Sbjct: 186 TAINPGPIDTNFFDIADQSGTYVKNMGRYMLKPTYVAEQIVKVMQTKKREVNLPKWMSMG 245 Query: 237 IRLYGLFPRLADKLTYNLMNRK 258 +++ LFP L +++ +++K Sbjct: 246 PKIFALFPGLFERIAGKSLSKK 267 >C3BPP4.1 C3BPP4_9BACI Uncharacterized oxidoreductase yqjQ Length = 267 Score = 264 bits (675), Expect = 5e-69, Method: Compositional matrix adjust. Identities = 127/260 (48%), Positives = 179/260 (68%), Gaps = 4/260 (1%) Query: 3 LQDKVVVITGASSGIGALTAQMLGKRGAIPILLARSEDKLKDTAAGIPGVFG----LYAC 58 LQ+KV+VITGASSGIG A + +GAIP+L+AR+E+KL+ A I + Y Sbjct: 8 LQEKVIVITGASSGIGEQVAMQVAAQGAIPVLIARTEEKLRVLAGKIKATYNTPCYYYVL 67 Query: 59 DVTDEEQVQNTFADILRVYGRIDVLLNNAGYGKFAAFTDMESREFDDMMDVNYMGIVRCT 118 DV++E ++ FA IL+ G+ID+L+NNAG+G F F + E DM VN G+V CT Sbjct: 68 DVSEEAEIDTVFARILKDAGQIDILVNNAGFGIFKTFEEASMTEVKDMFQVNVFGLVACT 127 Query: 119 KAVVPHMLERGSGQIVNVASMAGKIGTARSVAYTATKHAVLGFTNALRQELRRQGIIVSA 178 KAV+PHMLER SGQI+N+AS+AGKI T +S AY A+KHAVLGFTN+LR EL I V+A Sbjct: 128 KAVLPHMLERNSGQIINIASLAGKIATPKSSAYAASKHAVLGFTNSLRMELSNTNIHVTA 187 Query: 179 VNPGPIATEFFRTADPSGSYEKSVARMMMTPEHVSKKIVKLIDKGKEEIDLPGFAAGGIR 238 +NPGPI T FF AD SG+Y K++ R M+ P +V+++IVK++ K E++LP + + G + Sbjct: 188 INPGPIDTNFFDIADQSGTYVKNMGRYMLKPTYVAEQIVKVMQTKKREVNLPKWMSVGPK 247 Query: 239 LYGLFPRLADKLTYNLMNRK 258 L+ LFP +++ +++K Sbjct: 248 LFALFPGFFERIAGKSLSKK 267 >C3B7M8.1 C3B7M8_BACMY Uncharacterized oxidoreductase yqjQ Length = 267 Score = 264 bits (674), Expect = 6e-69, Method: Compositional matrix adjust. Identities = 127/260 (48%), Positives = 179/260 (68%), Gaps = 4/260 (1%) Query: 3 LQDKVVVITGASSGIGALTAQMLGKRGAIPILLARSEDKLKDTAAGIPGVFG----LYAC 58 LQ+KV+VITGASSGIG A + +GAIP+L+AR+E+KL+ A I + Y Sbjct: 8 LQEKVIVITGASSGIGEEVAMQVAAQGAIPVLIARTEEKLRVLAGKIKATYNTPCYYYVL 67 Query: 59 DVTDEEQVQNTFADILRVYGRIDVLLNNAGYGKFAAFTDMESREFDDMMDVNYMGIVRCT 118 DV++E ++ FA IL+ G+ID+L+NNAG+G F F + E DM VN G+V CT Sbjct: 68 DVSEEAEIDTVFARILKDAGQIDILVNNAGFGIFKTFEEASMTEVKDMFQVNVFGLVACT 127 Query: 119 KAVVPHMLERGSGQIVNVASMAGKIGTARSVAYTATKHAVLGFTNALRQELRRQGIIVSA 178 KAV+PHMLER SGQI+N+AS+AGKI T +S AY A+KHAVLGFTN+LR EL I V+A Sbjct: 128 KAVLPHMLERNSGQIINIASLAGKIATPKSSAYAASKHAVLGFTNSLRMELSNTNIHVTA 187 Query: 179 VNPGPIATEFFRTADPSGSYEKSVARMMMTPEHVSKKIVKLIDKGKEEIDLPGFAAGGIR 238 +NPGPI T FF AD SG+Y K++ R M+ P +V+++IVK++ K E++LP + + G + Sbjct: 188 INPGPIDTNFFDIADQSGTYVKNMGRYMLKPTYVAEQIVKVMQTKKREVNLPKWMSVGPK 247 Query: 239 LYGLFPRLADKLTYNLMNRK 258 L+ LFP +++ +++K Sbjct: 248 LFALFPGFFERIAGKSLSKK 267 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.137 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,448,788,832 Number of extensions: 57848422 Number of successful extensions: 251323 Number of sequences better than 1.0e-05: 32505 Number of HSP's gapped: 210218 Number of HSP's successfully gapped: 32587 Length of query: 258 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 125 Effective length of database: 1,807,701,208 Effective search space: 225962651000 Effective search space used: 225962651000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)