BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0434.0029.p (300 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KEI1.1 B3KEI1_9BACI LysR substrate-binding 491 e-137 B1DDK2.1 B1DDK2_9BACL Transcriptional regulator, LysR family 423 e-116 A9U6X8.1 A9U6X8_PHYPA Predicted protein (Fragment) 205 4e-51 Q67KY5.1 Q67KY5_SYMTH LysR family transcriptional regulator 167 2e-39 B4CZS1.1 B4CZS1_9BACT Transcriptional regulator, LysR family 152 3e-35 >B3KEI1.1 B3KEI1_9BACI LysR substrate-binding Length = 300 Score = 491 bits (1263), Expect = e-137, Method: Compositional matrix adjust. Identities = 235/300 (78%), Positives = 269/300 (89%) Query: 1 MNISQLETLITISKTMSFRKAGELLNLTQPAVSAQIKSLEEEFKTQLVDRNQPVTLTDRG 60 MNISQLETLITISKTMSFRKAGELLNLTQPAVSAQIKSLEEEFKT LVDR+QPVTLTDRG Sbjct: 1 MNISQLETLITISKTMSFRKAGELLNLTQPAVSAQIKSLEEEFKTVLVDRSQPVTLTDRG 60 Query: 61 QVFLTHAEQIVGLVEELKQRLADLDENPQGHIILGTTTSIAIQILPRILSYFQDQFPHIK 120 QVF+ HAEQI+G+V+ELKQRL+DLD+ PQGHI LGTTTSIAIQILPR+LSYFQDQFPHIK Sbjct: 61 QVFVDHAEQILGIVDELKQRLSDLDQTPQGHIQLGTTTSIAIQILPRVLSYFQDQFPHIK 120 Query: 121 TXXXXXXXXQIYQHVENGLVDVGIGYLIGRSPGMTTSVLYYDTFELVVSPRHPLAQVKSA 180 T QIY VENGL+D+GIGYLI R+P ++TSVLYYDTFELVVSP+HPLA++ +A Sbjct: 121 TTIQSMASSQIYHSVENGLIDIGIGYLIERNPNLSTSVLYYDTFELVVSPQHPLAKLSTA 180 Query: 181 GIEALRNTPLILLSPDTVGRRFADEVFAKHGIQPQVIMELTSSEEVKRMVELDLGAAVIS 240 ++ L TPLILL+ DTVGRRFADEV KHG+ PQV+MEL+SSEE+KRMVE++LGAA+IS Sbjct: 181 NMDVLAGTPLILLTRDTVGRRFADEVMKKHGVDPQVVMELSSSEEIKRMVEINLGAAIIS 240 Query: 241 KQSVTAEVRSGTLKIVPIIELEVTHPVGVITKSGKYVNSAMRQFLSDLKGMPETHFIGSE 300 KQSV E+R+GTL+IVP+ ELEV+HPVGVI KSG+YV+SAMRQFLSDLKGMPET FI SE Sbjct: 241 KQSVATELRNGTLQIVPLSELEVSHPVGVIYKSGRYVSSAMRQFLSDLKGMPETQFISSE 300 >B1DDK2.1 B1DDK2_9BACL Transcriptional regulator, LysR family Length = 300 Score = 423 bits (1087), Expect = e-116, Method: Compositional matrix adjust. Identities = 199/300 (66%), Positives = 249/300 (83%) Query: 1 MNISQLETLITISKTMSFRKAGELLNLTQPAVSAQIKSLEEEFKTQLVDRNQPVTLTDRG 60 MNISQLETL+TISKTMSFRKAGELLNLTQPAVSAQIK+LEEEFKT L+DRNQPVTLTD G Sbjct: 1 MNISQLETLLTISKTMSFRKAGELLNLTQPAVSAQIKTLEEEFKTVLIDRNQPVTLTDHG 60 Query: 61 QVFLTHAEQIVGLVEELKQRLADLDENPQGHIILGTTTSIAIQILPRILSYFQDQFPHIK 120 +VFL HAE+I+ +VEELKQ+L+DL+ PQGHI+LGTT+S+AIQILPR+LSYFQDQ+P IK Sbjct: 61 KVFLEHAERILAVVEELKQKLSDLNAIPQGHIVLGTTSSMAIQILPRVLSYFQDQYPLIK 120 Query: 121 TXXXXXXXXQIYQHVENGLVDVGIGYLIGRSPGMTTSVLYYDTFELVVSPRHPLAQVKSA 180 T Q+ VENG VD+GI Y+ ++P M +S+LYYD+F LV SP HPL+QV Sbjct: 121 TTIHTMPSSQVLASVENGSVDMGITYISEKNPNMDSSILYYDSFVLVASPAHPLSQVAHT 180 Query: 181 GIEALRNTPLILLSPDTVGRRFADEVFAKHGIQPQVIMELTSSEEVKRMVELDLGAAVIS 240 I+ +++ P I+L+PDT+GRRF D++F K+ IQP +IMEL+SSEEVKRMVE++LG AV+S Sbjct: 181 TIDMIKDYPFIMLAPDTLGRRFLDQIFKKNNIQPNIIMELSSSEEVKRMVEINLGIAVVS 240 Query: 241 KQSVTAEVRSGTLKIVPIIELEVTHPVGVITKSGKYVNSAMRQFLSDLKGMPETHFIGSE 300 K S+ E+R G+LKI+ + ELE HPV V+ KSG+Y+N+AM+QFLSDLKGMPE F+GSE Sbjct: 241 KLSIANELRLGSLKIIKVTELEPYHPVAVVYKSGRYLNTAMQQFLSDLKGMPEQSFLGSE 300 >A9U6X8.1 A9U6X8_PHYPA Predicted protein (Fragment) Length = 126 Score = 205 bits (522), Expect = 4e-51, Method: Compositional matrix adjust. Identities = 98/126 (77%), Positives = 110/126 (87%) Query: 16 MSFRKAGELLNLTQPAVSAQIKSLEEEFKTQLVDRNQPVTLTDRGQVFLTHAEQIVGLVE 75 MSFRKAGELLNLTQPAVSAQIKSLE+EFKT LVDRNQPVTLTDRG+VFL+HAE+I+ +VE Sbjct: 1 MSFRKAGELLNLTQPAVSAQIKSLEDEFKTVLVDRNQPVTLTDRGEVFLSHAERILAIVE 60 Query: 76 ELKQRLADLDENPQGHIILGTTTSIAIQILPRILSYFQDQFPHIKTXXXXXXXXQIYQHV 135 ELK+RL DLD+ PQGHI+LGTTTSIAIQILPR+LSYFQ+QFP IKT QIY V Sbjct: 61 ELKERLYDLDQTPQGHIVLGTTTSIAIQILPRVLSYFQNQFPLIKTSIMSMASSQIYTSV 120 Query: 136 ENGLVD 141 ENG +D Sbjct: 121 ENGQID 126 >Q67KY5.1 Q67KY5_SYMTH LysR family transcriptional regulator Length = 298 Score = 167 bits (422), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 96/295 (32%), Positives = 159/295 (53%), Gaps = 2/295 (0%) Query: 1 MNISQLETLITISKTMSFRKAGELLNLTQPAVSAQIKSLEEEFKTQLVDRN-QPVTLTDR 59 M +S L I +T SF KA ++QPAVS Q+K++EE F+ +L++R+ Q + T+ Sbjct: 1 MLLSYLRVYCDIIETRSFSKAAARNFISQPAVSQQVKAMEEHFQQRLIERSPQGIYPTEA 60 Query: 60 GQVFLTHAEQIVGLVEELKQRLADLDENPQGHIILGTTTSIAIQILPRILSYFQDQFPHI 119 G++F A +IV + L+Q++A+L ++ G I + T ++ + LP + F +P Sbjct: 61 GRIFYEAAREIVERYQALEQQMANLSQSVSGTIRVATVYTVGLHDLPPYIKKFIQTYPQA 120 Query: 120 KTXXXXXXXXQIYQHVENGLVDVGIGYLIGRSPGMTTSVLYYDTFELVVSPRHPLAQV-K 178 + +IY+ V VD+GI + L D ++VSP HPLA+ K Sbjct: 121 RIHLEYSRTNKIYEAVRQNQVDIGIVAYPQEGRQLGVIPLPSDELVVIVSPNHPLARAGK 180 Query: 179 SAGIEALRNTPLILLSPDTVGRRFADEVFAKHGIQPQVIMELTSSEEVKRMVELDLGAAV 238 ++ L P I PD R+ D V HG+ ++I E + E +KR VE +LG ++ Sbjct: 181 PVALQQLHRLPFIAFDPDIPTRKATDRVLNDHGVDVEIIHEFDNVETIKRSVEAELGVSI 240 Query: 239 ISKQSVTAEVRSGTLKIVPIIELEVTHPVGVITKSGKYVNSAMRQFLSDLKGMPE 293 I ++ EVRSG+L +PI ++ + PVGVI K GK ++ +++F++ L G E Sbjct: 241 IPRRCAQWEVRSGSLVALPIADMRIERPVGVIYKRGKNFSNTLQKFIAILTGREE 295 >B4CZS1.1 B4CZS1_9BACT Transcriptional regulator, LysR family Length = 305 Score = 152 bits (385), Expect = 3e-35, Method: Compositional matrix adjust. Identities = 91/290 (31%), Positives = 147/290 (50%), Gaps = 1/290 (0%) Query: 1 MNISQLETLITISKTMSFRKAGELLNLTQPAVSAQIKSLEEEFKTQLVDR-NQPVTLTDR 59 M I + +++T SF KA L +TQ AVS QI++LE +F+ LV+R + LT Sbjct: 1 MYIDTFKVFCDLAETGSFSKAAVLNAITQSAVSQQIRALENKFQVTLVERGRRNFALTAE 60 Query: 60 GQVFLTHAEQIVGLVEELKQRLADLDENPQGHIILGTTTSIAIQILPRILSYFQDQFPHI 119 G FL + +I+ + L RL +L + G + + SI + LP L F+++FP + Sbjct: 61 GNAFLEASREILEVYNNLDHRLHELRDVIAGELRIAAIYSIGLHELPSHLKAFREKFPDV 120 Query: 120 KTXXXXXXXXQIYQHVENGLVDVGIGYLIGRSPGMTTSVLYYDTFELVVSPRHPLAQVKS 179 + Q+Y V G VD+G+ + G+ + D L+ P HPLA K Sbjct: 121 ELHVEYRRSQQVYTQVLGGDVDIGLVAFPTKRNGLQIEIFSEDKLVLICHPNHPLAGRKK 180 Query: 180 AGIEALRNTPLILLSPDTVGRRFADEVFAKHGIQPQVIMELTSSEEVKRMVELDLGAAVI 239 IE L I PD R+ D F H I+ + ME + E VKR VE++ G +++ Sbjct: 181 IKIEELNGEKFIAFEPDLPTRKVIDRHFRDHQIEIEHAMEFDNIETVKRAVEIESGVSIV 240 Query: 240 SKQSVTAEVRSGTLKIVPIIELEVTHPVGVITKSGKYVNSAMRQFLSDLK 289 + +V EV SG L + I +++ P+G++TK + + A ++F++ LK Sbjct: 241 PRNTVRQEVESGVLSALDIEGPKMSRPLGILTKRNRARSPAHKEFVAALK 290 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.135 0.368 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,588,519,328 Number of extensions: 61475156 Number of successful extensions: 211259 Number of sequences better than 1.0e-05: 13280 Number of HSP's gapped: 206363 Number of HSP's successfully gapped: 13280 Length of query: 300 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 165 Effective length of database: 1,788,859,178 Effective search space: 295161764370 Effective search space used: 295161764370 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)