BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0434.0027.p (280 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q9K8Q0.1 Q9K8Q0_BACHD NADPH-flavin oxidoreductase 272 2e-71 Q65ND8.1 Q65ND8_BACLD YcnD 246 2e-63 A7Z1D4.1 A7Z1D4_BACA2 YcnD 236 1e-60 Q548A6.1 Q548A6_BACSU Oxidoreductase 232 3e-59 P94424.1 YCND_BACSU Uncharacterized oxidoreductase ycnD EC=1.-.-.- 232 3e-59 >Q9K8Q0.1 Q9K8Q0_BACHD NADPH-flavin oxidoreductase Length = 251 Score = 272 bits (696), Expect = 2e-71, Method: Compositional matrix adjust. Identities = 134/250 (53%), Positives = 171/250 (68%), Gaps = 3/250 (1%) Query: 33 LNEVIRTLMNHRSFRQYRQEPVEPEKLKTIIKAAQAAPSWVNGQQVSVIAVRSEERRQRL 92 +NEVI+T+MNHRS RQY +PV + L+ I+ AAQ PSW++GQQVSVI +R R+ Sbjct: 1 MNEVIQTMMNHRSCRQYSDKPVPDDHLQAILHAAQWGPSWIHGQQVSVIVIREPNERELF 60 Query: 93 SVLSGNQKHVAQAPVFLVFCMDFYRAKVAGELEGQPFEAAKDVDALLVGATDVGIALSNA 152 + L G Q HVAQAP+FLVFC DF+RA VA E G+ A +DVDAL+VGATDVGIALSNA Sbjct: 61 AELVGGQAHVAQAPIFLVFCADFHRAAVAAEARGKFLAALEDVDALMVGATDVGIALSNA 120 Query: 153 IAAAESLGLGIIPIGGVRRHTAEVIELLQLPEYVFPVVGLCVGYPAEELPKKPRLPLEAV 212 I AAESLGLG +PIGG+RR VIE LQLP YV P+ GLC+GYP E + +KPRLPL+ Sbjct: 121 ITAAESLGLGTVPIGGIRRQPLRVIETLQLPSYVIPISGLCIGYPEETVEQKPRLPLDVF 180 Query: 213 LHEEQYNPEVKSLIEAYNLAHRASLKEQ--GLTERDWSSTIAHFYTLNPQYGDAQHTLKR 270 H+ +Y P + + AY+ + K++ G T WS + FY P Y + LK+ Sbjct: 181 THDGRYEPLSQKTLAAYDELISGNTKQRTGGATTTTWSDRLISFYE-QPYYQEIVAMLKQ 239 Query: 271 QGFTCDNLKD 280 QGFTC N+K+ Sbjct: 240 QGFTCKNVKE 249 >Q65ND8.1 Q65ND8_BACLD YcnD Length = 245 Score = 246 bits (628), Expect = 2e-63, Method: Compositional matrix adjust. Identities = 121/246 (49%), Positives = 168/246 (68%), Gaps = 3/246 (1%) Query: 33 LNEVIRTLMNHRSFRQYRQEPVEPEKLKTIIKAAQAAPSWVNGQQVSVIAVRSEERRQRL 92 +NEV++TL +HRS R Y EPV PE+L II+A QAAP+ +NGQQV+VI V+ EER++++ Sbjct: 1 MNEVLKTLKDHRSIRSYTDEPVSPEQLDDIIQAVQAAPNSINGQQVTVITVQDEERKKKI 60 Query: 93 SVLSGNQKHVAQAPVFLVFCMDFYRAKVAGELEGQPFEAAKDVDALLVGATDVGIALSNA 152 + L+G Q + QAPVFL+FC DF RAK+A E G+ ++++LVGA D GIAL A Sbjct: 61 AELAGGQVWIEQAPVFLLFCADFNRAKIALEKNGETLAITDGMESVLVGAVDAGIALGTA 120 Query: 153 IAAAESLGLGIIPIGGVRRHTAEVIELLQLPEYVFPVVGLCVGYPAEELPKKPRLPLEAV 212 AAAES+GLG +PIG VR E+I+LL +PEYVFPV GL VG+PA+ KKPRLP +AV Sbjct: 121 TAAAESMGLGTVPIGAVRGRAEELIKLLNIPEYVFPVAGLVVGHPADRSAKKPRLPEQAV 180 Query: 213 LHEEQYNPEVKSLIEAYN--LAHRASLKEQGLTERDWSSTIAHFYTLNPQYGDAQHTLKR 270 H E Y P++K LI+AY+ ++ + + G R+WS I+ +Y Y + L++ Sbjct: 181 RHSETYQPDLKPLIDAYDEEISEYMTKRTNGQETRNWSQGISAYYN-KVYYPHIREMLEK 239 Query: 271 QGFTCD 276 QGF + Sbjct: 240 QGFKLE 245 >A7Z1D4.1 A7Z1D4_BACA2 YcnD Length = 248 Score = 236 bits (603), Expect = 1e-60, Method: Compositional matrix adjust. Identities = 124/249 (49%), Positives = 167/249 (67%), Gaps = 5/249 (2%) Query: 33 LNEVIRTLMNHRSFRQYRQEPVEPEKLKTIIKAAQAAPSWVNGQQVSVIAVRSEERRQRL 92 +NEV++ L +HRS R Y EPV E+L II+AAQAAP+ +NGQQ +VIAV+ +ER++++ Sbjct: 1 MNEVLQLLTDHRSIRSYTDEPVSDEQLDLIIQAAQAAPTSINGQQFTVIAVKDKERKKKI 60 Query: 93 SVLSGNQKHVAQAPVFLVFCMDFYRAKVA-GELEGQPFEAAKDVDALLVGATDVGIALSN 151 S L+G Q + QAPVFL+FC DF RAK+A E P E ++++LVGA DVGIAL Sbjct: 61 SELAGGQPWIDQAPVFLLFCADFNRAKIALEEYNDTPLEITNGLESVLVGAVDVGIALGT 120 Query: 152 AIAAAESLGLGIIPIGGVRRHTAEVIELLQLPEYVFPVVGLCVGYPAEELPKKPRLPLEA 211 A AAAES+GLG +PIG VR E+IELL+LP+YVFPV GL +G+PA+ KKPRLP A Sbjct: 121 ATAAAESMGLGTVPIGAVRGKPDELIELLKLPKYVFPVSGLVIGHPADRSAKKPRLPQAA 180 Query: 212 VLHEEQYNPE-VKSLIEAYNLAHRASLKEQ--GLTERDWSSTIAHFYTLNPQYGDAQHTL 268 VLH E Y E +++ IEAY+ +K++ G R+WS I +Y Y + L Sbjct: 181 VLHHETYQEEDLRTHIEAYDKQMSEYMKKRTDGQETRNWSQGITSYYK-QIYYPHIREML 239 Query: 269 KRQGFTCDN 277 ++QGF D Sbjct: 240 EKQGFKTDK 248 >Q548A6.1 Q548A6_BACSU Oxidoreductase Length = 249 Score = 232 bits (591), Expect = 3e-59, Method: Compositional matrix adjust. Identities = 122/249 (48%), Positives = 169/249 (67%), Gaps = 6/249 (2%) Query: 33 LNEVIRTLMNHRSFRQYRQEPVEPEKLKTIIKAAQAAPSWVNGQQVSVIAVRSEERRQRL 92 +NEVI++L +HRS R Y EPV E+L II+A Q+APS +NGQQV+VI V+ +ER++++ Sbjct: 1 MNEVIKSLTDHRSIRSYTDEPVAQEQLDQIIEAVQSAPSSINGQQVTVITVQDKERKKKI 60 Query: 93 SVLSGNQKHVAQAPVFLVFCMDFYRAKVAGE-LEGQPFEAAKDVDALLVGATDVGIALSN 151 S L+G Q + QAPVFL+FC DF RAK+A E L E ++++LVGA D GIAL Sbjct: 61 SELAGGQPWIDQAPVFLLFCADFNRAKIALEDLHDFKMEITNGLESVLVGAVDAGIALGT 120 Query: 152 AIAAAESLGLGIIPIGGVRRHTAEVIELLQLPEYVFPVVGLCVGYPAEELPKKPRLPLEA 211 A AAAESLGLG +PIG VR + E+IELL+LP+YVFP+ GL +G+PA+ KKPRLP EA Sbjct: 121 ATAAAESLGLGTVPIGAVRGNPQELIELLELPKYVFPLSGLVIGHPADRSAKKPRLPQEA 180 Query: 212 VLHEEQY--NPEVKSLIEAYN--LAHRASLKEQGLTERDWSSTIAHFYTLNPQYGDAQHT 267 V H+E Y E+ S I+AY+ ++ + + G R+WS +IA +Y Y + Sbjct: 181 VNHQETYLNQDELTSHIQAYDEQMSEYMNKRTNGKETRNWSQSIASYYE-RLYYPHIREM 239 Query: 268 LKRQGFTCD 276 L++QGF + Sbjct: 240 LEKQGFKVE 248 >P94424.1 YCND_BACSU Uncharacterized oxidoreductase ycnD EC=1.-.-.- Length = 249 Score = 232 bits (591), Expect = 3e-59, Method: Compositional matrix adjust. Identities = 122/249 (48%), Positives = 169/249 (67%), Gaps = 6/249 (2%) Query: 33 LNEVIRTLMNHRSFRQYRQEPVEPEKLKTIIKAAQAAPSWVNGQQVSVIAVRSEERRQRL 92 +NEVI++L +HRS R Y EPV E+L II+A Q+APS +NGQQV+VI V+ +ER++++ Sbjct: 1 MNEVIKSLTDHRSIRSYTDEPVAQEQLDQIIEAVQSAPSSINGQQVTVITVQDKERKKKI 60 Query: 93 SVLSGNQKHVAQAPVFLVFCMDFYRAKVAGE-LEGQPFEAAKDVDALLVGATDVGIALSN 151 S L+G Q + QAPVFL+FC DF RAK+A E L E ++++LVGA D GIAL Sbjct: 61 SELAGGQPWIDQAPVFLLFCADFNRAKIALEDLHDFKMEITNGLESVLVGAVDAGIALGT 120 Query: 152 AIAAAESLGLGIIPIGGVRRHTAEVIELLQLPEYVFPVVGLCVGYPAEELPKKPRLPLEA 211 A AAAESLGLG +PIG VR + E+IELL+LP+YVFP+ GL +G+PA+ KKPRLP EA Sbjct: 121 ATAAAESLGLGTVPIGAVRGNPQELIELLELPKYVFPLSGLVIGHPADRSAKKPRLPQEA 180 Query: 212 VLHEEQY--NPEVKSLIEAYN--LAHRASLKEQGLTERDWSSTIAHFYTLNPQYGDAQHT 267 V H+E Y E+ S I+AY+ ++ + + G R+WS +IA +Y Y + Sbjct: 181 VNHQETYLNQDELTSHIQAYDEQMSEYMNKRTNGKETRNWSQSIASYYE-RLYYPHIREM 239 Query: 268 LKRQGFTCD 276 L++QGF + Sbjct: 240 LEKQGFKVE 248 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.135 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,655,772,510 Number of extensions: 66431310 Number of successful extensions: 190511 Number of sequences better than 1.0e-05: 795 Number of HSP's gapped: 189879 Number of HSP's successfully gapped: 795 Length of query: 280 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 146 Effective length of database: 1,798,280,193 Effective search space: 262548908178 Effective search space used: 262548908178 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)