BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0434.0025.p (311 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KEI3.1 B3KEI3_9BACI Metallophosphoesterase 300 1e-79 C0ZC23.1 C0ZC23_BREBN Putative uncharacterized protein ykuE 238 4e-61 A7GS19.1 A7GS19_BACCN Metallophosphoesterase 219 3e-55 B7GIC7.1 B7GIC7_ANOFW Predicted phosphohydrolase 216 2e-54 C2WBC7.1 C2WBC7_BACCE Metallophosphoesterase 214 9e-54 >B3KEI3.1 B3KEI3_9BACI Metallophosphoesterase Length = 302 Score = 300 bits (769), Expect = 1e-79, Method: Compositional matrix adjust. Identities = 139/246 (56%), Positives = 179/246 (72%) Query: 64 EPNWLDITRLELSFKELPSAFAGTRLVHFSDVHLGFNKDVHDLKRLAGHIKEAQPDIICF 123 EP LDITR L+F LP AF G ++V FSD+HLGF+ + R+ I +PD+ICF Sbjct: 54 EPRQLDITRHTLTFPSLPKAFDGMKIVQFSDLHLGFHAGADNAARVVQAIHHEKPDMICF 113 Query: 124 TGDIVDSYAEDLTESVPVLAGLSAPLGKYAILGNHDYKNTELLTRLLNEAGFRVLRNQSY 183 TGD+VD AED+ ++ LA L APLG ++ILGNHDY + + L L EAGF+VLRN + Sbjct: 114 TGDMVDGNAEDMRAAIQPLAQLKAPLGLFSILGNHDYGDVKKLIALEEEAGFQVLRNDAV 173 Query: 184 LVKQGGASIAVTGLDDMLHGKPDPQAALKDVPEGMFTLLMMHEPDYADTVEAYPFHLQLS 243 +++ GA AV GLDD + G PDP+AA++D+PEGMF LL+MHEPDYADTV YPFHLQLS Sbjct: 174 KLRREGAVFAVAGLDDGIWGTPDPEAAIRDLPEGMFKLLLMHEPDYADTVAVYPFHLQLS 233 Query: 244 GHSHGGQIRLPFVGAAYTPYGSNKYISGLYYTDKKRMPVYVNRGFGETYMPFRLMCRPEL 303 GHSHGGQIRLP++G TP G+ +Y+ GLY + M +YVNRG G T++PFR +C+PEL Sbjct: 234 GHSHGGQIRLPWMGEVITPPGAKRYVQGLYTVGSQGMQLYVNRGIGTTHLPFRFLCKPEL 293 Query: 304 TIFTLR 309 T+ TLR Sbjct: 294 TVLTLR 299 >C0ZC23.1 C0ZC23_BREBN Putative uncharacterized protein ykuE Length = 285 Score = 238 bits (608), Expect = 4e-61, Method: Compositional matrix adjust. Identities = 119/253 (47%), Positives = 161/253 (63%), Gaps = 6/253 (2%) Query: 59 YAWKGEPNWLDITRLELSFKELPSAFAGTRLVHFSDVHLGFNKDVHDLKRLAGHIKEAQP 118 + W E LDI RL ++ LP +F GT+L+HFSDVHLG + +++ + I+ +P Sbjct: 37 HVW--ERKALDIVRLSITIPGLPESFKGTKLIHFSDVHLGHYFEPKEMEPVIAAIQSEKP 94 Query: 119 DIICFTGDIVDSYAEDLTESVPVLAGLSAPLGKYAILGNHDYKNTEL--LTRLLNEAGFR 176 D+ICFTGDIVD L +VP+ L APLGK+A+LGNHDY+ E + L +GF Sbjct: 95 DLICFTGDIVDEVTRPLFAAVPLFNQLLAPLGKFAVLGNHDYRAGEQQKVRDGLIASGFE 154 Query: 177 VLRNQSYLVKQGGASIAVTGLDDMLHGKPDPQAALKDVPEGMFTLLMMHEPDYADTVEAY 236 VL N+ +V +GG + + G+DD+ +G PD AL+++P +L+ HEPD+AD + Sbjct: 155 VLDNRHVVVHKGGQQLYMAGVDDIFYGVPDLSRALENIPPKGSVILLAHEPDFADIASEH 214 Query: 237 PFHLQLSGHSHGGQIRLPFVGAAYTPYGSNKYISGLYYTDKKRMPVYVNRGFGETYMPFR 296 P HLQLSGHSHGGQ+RLPF+G TP KY+ GLY M VY NRG G T +P R Sbjct: 215 PVHLQLSGHSHGGQVRLPFIGHLMTPQYGRKYVQGLYRVGG--MAVYTNRGLGTTILPVR 272 Query: 297 LMCRPELTIFTLR 309 L CRPELT+ TL+ Sbjct: 273 LFCRPELTVLTLQ 285 >A7GS19.1 A7GS19_BACCN Metallophosphoesterase Length = 287 Score = 219 bits (558), Expect = 3e-55, Method: Compositional matrix adjust. Identities = 105/258 (40%), Positives = 163/258 (63%), Gaps = 7/258 (2%) Query: 59 YAWKGEPNWLDITRLELSFKELPSAFAGTRLVHFSDVHLGFNKDVHDLKRLAGHIKEAQP 118 YA EPN+L T L +P++F G +++ FSD+HLG+ + L + I +A P Sbjct: 30 YAKYIEPNFLSFTHYTLQSPLIPNSFHGMKIIQFSDLHLGYYFSLQRLSSIVSKINDATP 89 Query: 119 DIICFTGDIVDSYAE--DLTESVPVLAGLSAPLGKYAILGNHDY--KNTELLTRLLNEAG 174 DI+ FTGD++D+Y + +L + AP GK+AI GNHD+ TE +++NE+G Sbjct: 90 DIVLFTGDLIDNYQTYGETPFVSAILRNIRAPFGKFAIYGNHDHGGYGTEYYQQIMNESG 149 Query: 175 FRVLRNQSYLVKQ-GGASIAVTGLDDMLHGKPDPQAALKDVPEGMFTLLMMHEPDYADTV 233 F +L+N+ ++ G I++ G+DDML GKP + L+ + ++T++++HEPD A V Sbjct: 150 FELLQNKEKRIRLFDGTEISIVGIDDMLLGKPKIEETLQHIKHNIYTIVLVHEPDIASKV 209 Query: 234 EAYPFHLQLSGHSHGGQIRLPFVGAAYTPYGSNKYISGLYYTDKKRMPVYVNRGFGETYM 293 +P ++QLSGHSHGGQ+++PF+GA T + +YI G Y+ D + +YVNRG G T + Sbjct: 210 AKFPVNVQLSGHSHGGQVQIPFLGAVVTSTLAKQYIEGFYHIDD--LVLYVNRGLGRTRI 267 Query: 294 PFRLMCRPELTIFTLRRA 311 PFR M RPE+T+FTL+ Sbjct: 268 PFRFMARPEITLFTLQHT 285 >B7GIC7.1 B7GIC7_ANOFW Predicted phosphohydrolase Length = 293 Score = 216 bits (550), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 105/255 (41%), Positives = 159/255 (62%), Gaps = 6/255 (2%) Query: 59 YAWKGEPNWLDITRLELSFKELPSAFAGTRLVHFSDVHLGFNKDVHDLKRLAGHIKEAQP 118 YA EP L + R ++ +P +F G RL+ FSDVHLG++ V +R+ + + P Sbjct: 38 YAKYVEPQQLKVRRYDVVHPLIPKSFDGCRLLQFSDVHLGYHYSVDHFQRVVKRMNDLDP 97 Query: 119 DIICFTGDIVD--SYAEDLTESVPVLAGLSAPLGKYAILGNHDYKN--TELLTRLLNEAG 174 +ICFTGD++D + E + +L L APLGK+ I GNHD+ T++ R++ +G Sbjct: 98 HVICFTGDLLDEPNQYEHVDAITSLLTQLHAPLGKFCIYGNHDHGGYGTDIYKRMMTASG 157 Query: 175 FRVLRNQSYLVKQGGASIAVTGLDDMLHGKPDPQAALKDVPEGMFTLLMMHEPDYADTVE 234 FR+L N+ +++G + + GLDD + GKP+ ++ +P+ +T+ ++HEPD Sbjct: 158 FRLLVNEWVTIERGHERMTIAGLDDFMLGKPNFAKTVQQLPKETYTIALIHEPDLVTQAS 217 Query: 235 AYPFHLQLSGHSHGGQIRLPFVGAAYTPYGSNKYISGLYYTDKKRMPVYVNRGFGETYMP 294 YPFHLQLSGHSHGGQI+LP++G TP + +Y+ G Y D ++ +YVNRG G T MP Sbjct: 218 VYPFHLQLSGHSHGGQIQLPWIGPLVTPPLAKQYVEGFY--DIGQLTLYVNRGLGTTRMP 275 Query: 295 FRLMCRPELTIFTLR 309 FR + PELT+FTL+ Sbjct: 276 FRFLTPPELTLFTLK 290 >C2WBC7.1 C2WBC7_BACCE Metallophosphoesterase Length = 285 Score = 214 bits (544), Expect = 9e-54, Method: Compositional matrix adjust. Identities = 105/257 (40%), Positives = 165/257 (64%), Gaps = 9/257 (3%) Query: 59 YAWKGEPNWLDITRLELSFKELPSAFAGTRLVHFSDVHLGFNKDVHDLKRLAGHIKEAQP 118 YA EPN+L +T L +P F G +++ FSD+HLG+ + L + I + P Sbjct: 30 YAKYIEPNFLSLTHHTLRSSLIPKNFHGMKIIQFSDLHLGYYFSLQHLSTIVSKINKTAP 89 Query: 119 DIICFTGDIVDSYAEDLTES---VPVLAGLSAPLGKYAILGNHDY--KNTELLTRLLNEA 173 DI+ FTGD++D Y + +E+ +L + AP GK+AI GNHD+ TE +++N+A Sbjct: 90 DIVLFTGDLIDDY-QTYSETPFVSAILRNIRAPFGKFAIYGNHDHGGYGTEYYKQIMNDA 148 Query: 174 GFRVLRNQSYLVKQ-GGASIAVTGLDDMLHGKPDPQAALKDVPEGMFTLLMMHEPDYADT 232 GF VL+N ++ + I++ G+DD+L GKP + L+ V + ++T++++HEPD A Sbjct: 149 GFEVLQNTEKRIRLLDNSEISIFGIDDILLGKPKISSTLQHVQKELYTIVLVHEPDIASE 208 Query: 233 VEAYPFHLQLSGHSHGGQIRLPFVGAAYTPYGSNKYISGLYYTDKKRMPVYVNRGFGETY 292 + +P +LQLSGHSHGGQ++LPF+GA TP + +YI G Y+ ++ + +YVNRG G T Sbjct: 209 IANFPVNLQLSGHSHGGQVQLPFLGAIVTPALAKRYIEGFYHIEE--LLLYVNRGLGRTR 266 Query: 293 MPFRLMCRPELTIFTLR 309 +PFR + RPE+T+FTL+ Sbjct: 267 VPFRFLSRPEITVFTLQ 283 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.138 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,939,326,732 Number of extensions: 81805617 Number of successful extensions: 178629 Number of sequences better than 1.0e-05: 1174 Number of HSP's gapped: 175868 Number of HSP's successfully gapped: 1186 Length of query: 311 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 176 Effective length of database: 1,788,859,178 Effective search space: 314839215328 Effective search space used: 314839215328 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)