BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0434.0015.p (453 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DDL3.1 B1DDL3_9BACL Potassium uptake protein, TrkH family EC=3... 608 e-172 C3J481.1 C3J481_9BACI Potassium uptake protein, TrkH family EC=3... 523 e-146 B7Y5K8.1 B7Y5K8_9BACI Potassium uptake protein, TrkH family EC=3... 523 e-146 Q5L3I3.1 Q5L3I3_GEOKA K+ uptake transporter (Trk family) 522 e-146 C5D5C4.1 C5D5C4_9BACI Potassium uptake protein, TrkH family EC=3... 518 e-145 >B1DDL3.1 B1DDL3_9BACL Potassium uptake protein, TrkH family EC=3.6.3.14 Length = 446 Score = 608 bits (1568), Expect = e-172, Method: Compositional matrix adjust. Identities = 300/443 (67%), Positives = 367/443 (82%), Gaps = 1/443 (0%) Query: 11 FRFLKLSPPQILVLGFAAVILIGTLLLMLPVSSTSGESLNFIDALFTATSATCVTGLVVV 70 F L+ SPP+ILV GFA +I+IG +LL +P++S+ G L FIDALFTATSATCVTGLVVV Sbjct: 5 FNILRWSPPRILVFGFAMIIVIGAVLLSMPIASSDGSRLPFIDALFTATSATCVTGLVVV 64 Query: 71 DTGTYFTVFGQTVIMVLIQVGGLGFMTMATLFALVLKRRISLRDRLILQEAMNQSSMEGI 130 DTGTYF+ FG+ VI+ LIQ+GGLGFMTM+TL ALVL++RISL++RLILQEAMNQ+SMEGI Sbjct: 65 DTGTYFSTFGKIVILSLIQIGGLGFMTMSTLIALVLRKRISLKERLILQEAMNQTSMEGI 124 Query: 131 VRLIRKVLIYSLVIEAGAAVILSMRWAFDMPFGRAVYFGIFHAVSMFNNAGFDIFGDYRS 190 VRLIR+V+ YSL IE A + ++R+AFDMPFGRA +FG+FH++S FNNAGFDIFG +RS Sbjct: 125 VRLIRRVIFYSLTIELVGAALFAIRFAFDMPFGRAAWFGLFHSISFFNNAGFDIFGHFRS 184 Query: 191 LTGYVSDPIVNIVVMFLIVSGGIGFIVMSDLVDYRRRTRRLSLHSKVVLSMTAALIVIGT 250 LT YV DP+VNIV M LI+ GG+GF+VM+DL+D+R+ +RLSLHSKVVLS T LI G Sbjct: 185 LTMYVGDPLVNIVSMLLIIFGGLGFVVMADLLDFRKN-KRLSLHSKVVLSTTGILIAAGA 243 Query: 251 VVIFVFEFTNTRTLGPLNFGGKIWAAFFQSVTPRTAGANTVDIAGLRQASQFFIVILMFI 310 +VIF+FEF+N+ TLG L++G KI AAFFQSVTPRTAGANT+D LRQA+ FF VILMFI Sbjct: 244 LVIFIFEFSNSNTLGSLHWGEKILAAFFQSVTPRTAGANTLDYTQLRQATVFFTVILMFI 303 Query: 311 GASPGSTGGGIKTTTFTLMIGAVISMLRGREDIVLFRYRLAQERVFKXXXXXXXXXXXXX 370 GASPGSTGGGIKTTTFT M+GAVI+M+RGR+DIVLF YRL ++R+FK Sbjct: 304 GASPGSTGGGIKTTTFTTMVGAVIAMIRGRDDIVLFHYRLGKDRIFKALTITMLSLTLVI 363 Query: 371 XXSMALSTTEDRAFLMILFETTSAFATVGLSMGLTPELSQAGKILICLTMFAGRLGLLTL 430 +M LSTTED+ FL ILFETTSAF TVGL++G+TPEL+ GK++I LTMFAGRLG LTL Sbjct: 364 FVTMLLSTTEDQQFLKILFETTSAFGTVGLTIGITPELTFFGKMVIALTMFAGRLGPLTL 423 Query: 431 AYAIGPKQGKQLYKYPEGKMIIG 453 AYA+GPK ++LY+YPEGK+ IG Sbjct: 424 AYALGPKTERELYRYPEGKITIG 446 >C3J481.1 C3J481_9BACI Potassium uptake protein, TrkH family EC=3.6.3.14 Length = 445 Score = 523 bits (1347), Expect = e-146, Method: Compositional matrix adjust. Identities = 258/438 (58%), Positives = 342/438 (78%), Gaps = 2/438 (0%) Query: 17 SPPQILVLGFAAVILIGTLLLMLPVSSTSGESLNFIDALFTATSATCVTGLVVVDTGTYF 76 PP++LV GFA +IL+G LLMLP ++ G+ L F+DALFTATSATCVTGLVVVDTGT F Sbjct: 9 DPPKVLVSGFALIILVGAFLLMLPTATVDGKGLPFLDALFTATSATCVTGLVVVDTGTTF 68 Query: 77 TVFGQTVIMVLIQVGGLGFMTMATLFALVLKRRISLRDRLILQEAMNQSSMEGIVRLIRK 136 T+FGQ VI+ LIQVGGLGFMT ATLFA +L +RISL++RL+LQEA+NQ S+EGIVRL+++ Sbjct: 69 TLFGQLVILALIQVGGLGFMTFATLFAFLLGKRISLKERLLLQEALNQLSIEGIVRLVKR 128 Query: 137 VLIYSLVIEAGAAVILSMRWAFDMPFGRAVYFGIFHAVSMFNNAGFDIFGDYRSLTGYVS 196 +L++++VIE ++L++R++FDMPF +AVYFG FHA+S FNNAGFD+ G++RSLTGYV Sbjct: 129 ILVFTVVIEGIGGLLLAIRFSFDMPFWKAVYFGFFHAISNFNNAGFDLMGEFRSLTGYVE 188 Query: 197 DPIVNIVVMFLIVSGGIGFIVMSDLVDYRRRTRRLSLHSKVVLSMTAALIVIGTVVIFVF 256 DP+V++VV LI+ GGIGFIVM+++ +Y R+TRRLSLH+KVV+ TA L+ +I + Sbjct: 189 DPVVSLVVPILIILGGIGFIVMNEVYEY-RQTRRLSLHTKVVVITTAWLVFASMALILLL 247 Query: 257 EFTNTRTLGPLNFGGKIWAAFFQSVTPRTAGANTVDIAGLRQASQFFIVILMFIGASPGS 316 E+ N +T+GPL+ GK +AF+Q+VTPRTAG+NT++I L Q + F IV LMF+GASPGS Sbjct: 248 EWNNPKTMGPLSLSGKFLSAFYQAVTPRTAGSNTLNIPDLTQPTLFLIVFLMFVGASPGS 307 Query: 317 TGGGIKTTTFTLMIGAVISMLRGREDIVLFRYRLAQERVFKXXXXXXXXXXXXXXXSMAL 376 TGGGIKTTTFT ++GAV S +RG+ED++LFR R+ + V+K +M L Sbjct: 308 TGGGIKTTTFTTLLGAVWSQIRGKEDVILFRKRIVYDTVYKSLTVTMSGLFVVMLVTMVL 367 Query: 377 STTED-RAFLMILFETTSAFATVGLSMGLTPELSQAGKILICLTMFAGRLGLLTLAYAIG 435 + TE + F MILFE TSAF TVGLSMGLTPELS GK +I TMFAGR+G LT+AYA+ Sbjct: 368 TITEKGKDFFMILFEATSAFGTVGLSMGLTPELSPLGKAIIIFTMFAGRVGPLTIAYAVT 427 Query: 436 PKQGKQLYKYPEGKMIIG 453 ++ ++YP+GKM+IG Sbjct: 428 LRRQPDPFRYPKGKMMIG 445 >B7Y5K8.1 B7Y5K8_9BACI Potassium uptake protein, TrkH family EC=3.6.3.14 Length = 445 Score = 523 bits (1347), Expect = e-146, Method: Compositional matrix adjust. Identities = 258/438 (58%), Positives = 342/438 (78%), Gaps = 2/438 (0%) Query: 17 SPPQILVLGFAAVILIGTLLLMLPVSSTSGESLNFIDALFTATSATCVTGLVVVDTGTYF 76 PP++LV GFA +IL+G LLMLP ++ G+ L F+DALFTATSATCVTGLVVVDTGT F Sbjct: 9 DPPKVLVSGFALIILVGAFLLMLPTATVDGKGLPFLDALFTATSATCVTGLVVVDTGTTF 68 Query: 77 TVFGQTVIMVLIQVGGLGFMTMATLFALVLKRRISLRDRLILQEAMNQSSMEGIVRLIRK 136 T+FGQ VI+ LIQVGGLGFMT ATLFA +L +RISL++RL+LQEA+NQ S+EGIVRL+++ Sbjct: 69 TLFGQLVILALIQVGGLGFMTFATLFAFLLGKRISLKERLLLQEALNQLSIEGIVRLVKR 128 Query: 137 VLIYSLVIEAGAAVILSMRWAFDMPFGRAVYFGIFHAVSMFNNAGFDIFGDYRSLTGYVS 196 +L++++VIE ++L++R++FDMPF +AVYFG FHA+S FNNAGFD+ G++RSLTGYV Sbjct: 129 ILVFTVVIEGIGGLLLAIRFSFDMPFWKAVYFGFFHAISNFNNAGFDLMGEFRSLTGYVE 188 Query: 197 DPIVNIVVMFLIVSGGIGFIVMSDLVDYRRRTRRLSLHSKVVLSMTAALIVIGTVVIFVF 256 DP+V++VV LI+ GGIGFIVM+++ +Y R+TRRLSLH+KVV+ TA L+ +I + Sbjct: 189 DPVVSLVVPILIILGGIGFIVMNEVYEY-RQTRRLSLHTKVVVITTAWLVFASMALILLL 247 Query: 257 EFTNTRTLGPLNFGGKIWAAFFQSVTPRTAGANTVDIAGLRQASQFFIVILMFIGASPGS 316 E+ N +T+GPL+ GK +AF+Q+VTPRTAG+NT++I L Q + F IV LMF+GASPGS Sbjct: 248 EWNNPKTMGPLSLSGKFLSAFYQAVTPRTAGSNTLNIPDLTQPTLFLIVFLMFVGASPGS 307 Query: 317 TGGGIKTTTFTLMIGAVISMLRGREDIVLFRYRLAQERVFKXXXXXXXXXXXXXXXSMAL 376 TGGGIKTTTFT ++GAV S +RG+ED++LFR R+ + V+K +M L Sbjct: 308 TGGGIKTTTFTTLLGAVWSQIRGKEDVILFRKRIVYDTVYKSLTVTMSGLFVVMLVTMVL 367 Query: 377 STTED-RAFLMILFETTSAFATVGLSMGLTPELSQAGKILICLTMFAGRLGLLTLAYAIG 435 + TE + F MILFE TSAF TVGLSMGLTPELS GK +I TMFAGR+G LT+AYA+ Sbjct: 368 TITEKGKDFFMILFEATSAFGTVGLSMGLTPELSPLGKAIIIFTMFAGRVGPLTIAYAVT 427 Query: 436 PKQGKQLYKYPEGKMIIG 453 ++ ++YP+GKM+IG Sbjct: 428 LRRQPDPFRYPKGKMMIG 445 >Q5L3I3.1 Q5L3I3_GEOKA K+ uptake transporter (Trk family) Length = 445 Score = 522 bits (1344), Expect = e-146, Method: Compositional matrix adjust. Identities = 259/438 (59%), Positives = 343/438 (78%), Gaps = 2/438 (0%) Query: 17 SPPQILVLGFAAVILIGTLLLMLPVSSTSGESLNFIDALFTATSATCVTGLVVVDTGTYF 76 PP++LV GFA +IL+G LLMLP ++ G+ L F+DALFTATSATCVTGLVVVDTGT F Sbjct: 9 DPPKVLVSGFALIILVGAFLLMLPTATVDGKGLPFLDALFTATSATCVTGLVVVDTGTTF 68 Query: 77 TVFGQTVIMVLIQVGGLGFMTMATLFALVLKRRISLRDRLILQEAMNQSSMEGIVRLIRK 136 T+FGQ VI+ LIQVGGLGFMT ATLFA +L +RISL++RL+LQEA+NQ S+EGIVRL+++ Sbjct: 69 TLFGQLVILALIQVGGLGFMTFATLFAFLLGKRISLKERLLLQEALNQLSIEGIVRLVKR 128 Query: 137 VLIYSLVIEAGAAVILSMRWAFDMPFGRAVYFGIFHAVSMFNNAGFDIFGDYRSLTGYVS 196 +L++++VIE ++L++R++FDMPF +AVYFG FHA+S FNNAGFD+ G++RSLTGYV Sbjct: 129 ILVFTVVIEGIGGLLLAIRFSFDMPFWKAVYFGFFHAISNFNNAGFDLMGEFRSLTGYVD 188 Query: 197 DPIVNIVVMFLIVSGGIGFIVMSDLVDYRRRTRRLSLHSKVVLSMTAALIVIGTVVIFVF 256 DP+V++VV LI+ GGIGFIVM+++ +Y R+T RLSLH+KVV TA L+V +I + Sbjct: 189 DPVVSLVVPILIILGGIGFIVMNEVYEY-RQTSRLSLHTKVVAITTAWLLVASMALILLL 247 Query: 257 EFTNTRTLGPLNFGGKIWAAFFQSVTPRTAGANTVDIAGLRQASQFFIVILMFIGASPGS 316 E+ N +T+GPL+ GK +AF+Q+VTPRTAG+NT++I L Q + F IV LMF+GASPGS Sbjct: 248 EWNNPKTMGPLSLSGKFLSAFYQAVTPRTAGSNTLNIPDLTQPTLFLIVFLMFVGASPGS 307 Query: 317 TGGGIKTTTFTLMIGAVISMLRGREDIVLFRYRLAQERVFKXXXXXXXXXXXXXXXSMAL 376 TGGGIKTTTFT ++GAV S +RG+ED++LFR R+ + V+K +M L Sbjct: 308 TGGGIKTTTFTTLLGAVWSQIRGKEDVILFRKRIVYDTVYKSLTVTMSGLFIVMFVTMVL 367 Query: 377 STTED-RAFLMILFETTSAFATVGLSMGLTPELSQAGKILICLTMFAGRLGLLTLAYAIG 435 + TE + FLMILFE TSAF TVGLSMGLTPELS GK +I LTMFAGR+G LT+AYA+ Sbjct: 368 TITESGKDFLMILFEATSAFGTVGLSMGLTPELSPLGKAVIILTMFAGRVGPLTIAYAVT 427 Query: 436 PKQGKQLYKYPEGKMIIG 453 ++ ++YP+GK++IG Sbjct: 428 LRRQPDPFRYPKGKIMIG 445 >C5D5C4.1 C5D5C4_9BACI Potassium uptake protein, TrkH family EC=3.6.3.14 Length = 446 Score = 518 bits (1333), Expect = e-145, Method: Compositional matrix adjust. Identities = 254/447 (56%), Positives = 345/447 (77%), Gaps = 2/447 (0%) Query: 8 INNFRFLKLSPPQILVLGFAAVILIGTLLLMLPVSSTSGESLNFIDALFTATSATCVTGL 67 +N R L PP+ILVLGF+ +ILIG LL LP ++ G+ L ++DALFTATSATCVTGL Sbjct: 1 MNKKRMFYLDPPKILVLGFSFIILIGAFLLTLPAATVDGKGLPWLDALFTATSATCVTGL 60 Query: 68 VVVDTGTYFTVFGQTVIMVLIQVGGLGFMTMATLFALVLKRRISLRDRLILQEAMNQSSM 127 VVVDTGT FT+FGQ VI+ LIQ+GGLGFM ATLFAL+L +RISL++RL+++E++N S+ Sbjct: 61 VVVDTGTTFTLFGQLVILALIQIGGLGFMAFATLFALILGKRISLKERLLIKESLNNLSI 120 Query: 128 EGIVRLIRKVLIYSLVIEAGAAVILSMRWAFDMPFGRAVYFGIFHAVSMFNNAGFDIFGD 187 +G+VRL++++LI++ VIE ++L++R++FDMP +A+YFG FHA+S FNNAGFD+ GD Sbjct: 121 DGVVRLVKRILIFTAVIELIGGILLAIRFSFDMPLPKAIYFGFFHAISNFNNAGFDLMGD 180 Query: 188 YRSLTGYVSDPIVNIVVMFLIVSGGIGFIVMSDLVDYRRRTRRLSLHSKVVLSMTAALIV 247 +RSLTGYV DP+V +VV LI GGIGFIVM+++ +Y R+TRR SLH+K+V M++ L+V Sbjct: 181 FRSLTGYVDDPLVTLVVCTLITLGGIGFIVMNEVYEY-RQTRRFSLHTKIVFVMSSILVV 239 Query: 248 IGTVVIFVFEFTNTRTLGPLNFGGKIWAAFFQSVTPRTAGANTVDIAGLRQASQFFIVIL 307 GT++IF+ E+ N +TL PL+ GK A+ +Q+VTPRTAG+NT++I L Q + F I+ L Sbjct: 240 FGTILIFILEYHNPKTLQPLSPLGKFLASLYQAVTPRTAGSNTLNIPDLTQPTLFLIIFL 299 Query: 308 MFIGASPGSTGGGIKTTTFTLMIGAVISMLRGREDIVLFRYRLAQERVFKXXXXXXXXXX 367 MFIGASPGSTGGGIKTTTF ++GAV S ++G+ED++ FR R+ + ++K Sbjct: 300 MFIGASPGSTGGGIKTTTFATLLGAVWSNIKGKEDVIFFRNRILYDTIYKALTVTVSGLF 359 Query: 368 XXXXXSMALSTTE-DRAFLMILFETTSAFATVGLSMGLTPELSQAGKILICLTMFAGRLG 426 +M L+ TE ++ FLMILFE TSAFATVGLSMGLTPELS GKILI LTMFAGR+G Sbjct: 360 VVMMITMLLTITEPNKDFLMILFEATSAFATVGLSMGLTPELSPVGKILIILTMFAGRVG 419 Query: 427 LLTLAYAIGPKQGKQLYKYPEGKMIIG 453 LT+AYA+ + +K+P+GK++IG Sbjct: 420 PLTIAYAVTLHRKPDPFKFPKGKIMIG 446 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.329 0.143 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,270,153,704 Number of extensions: 84965273 Number of successful extensions: 312502 Number of sequences better than 1.0e-05: 1184 Number of HSP's gapped: 306565 Number of HSP's successfully gapped: 1199 Length of query: 453 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 314 Effective length of database: 1,751,175,118 Effective search space: 549868987052 Effective search space used: 549868987052 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 133 (55.8 bits)