BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0434.0011.p (272 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KFC5.1 B3KFC5_9BACI 2,5-didehydrogluconate reductase EC=1.1.1.274 464 e-129 C4C5F0.1 C4C5F0_9FUSO Aldo/keto reductase, diketogulonate reduct... 392 e-107 B1DF75.1 B1DF75_9BACL 2,5-didehydrogluconate reductase EC=1.1.1.274 386 e-105 B3CCU7.1 B3CCU7_9BACE Putative uncharacterized protein 385 e-105 B2V3I3.1 B2V3I3_CLOBA Morphine 6-dehydrogenase EC=1.1.1.218 379 e-103 >B3KFC5.1 B3KFC5_9BACI 2,5-didehydrogluconate reductase EC=1.1.1.274 Length = 283 Score = 464 bits (1193), Expect = e-129, Method: Compositional matrix adjust. Identities = 220/272 (80%), Positives = 236/272 (86%) Query: 1 MPIIGFGVYQVPDAEECENAVYEALMAGYRLIDTAAGYLNEEAVGRAIKRSGVPREELFI 60 MPI+GFGVYQ+PD +ECENAVYEAL GYRLIDTAAGYLNEEAVGRAIKRSGVPREELFI Sbjct: 12 MPILGFGVYQIPDTDECENAVYEALSVGYRLIDTAAGYLNEEAVGRAIKRSGVPREELFI 71 Query: 61 TTKLWVQDAGYESXXXXXXXXXXXXXXXXXXXXXIHQPFGDYYGAWRAMEDLYREGKIKA 120 TTK+WVQDAGYES IHQPFGDYYG WRAME+LYR+GKIKA Sbjct: 72 TTKIWVQDAGYESAKLAFAKSLKKLQLDYLDLYLIHQPFGDYYGTWRAMEELYRDGKIKA 131 Query: 121 IGVSNFLPDRLMDLIVHNEIVPAVNQIETHPFYQQTESAAFMKEQGVQHQSWAPFAEGRN 180 IGVSNF+PDRLMDLIVHNEIVPAVNQIETHPFYQQ ESAAF+KEQGVQHQSWAPFAEGRN Sbjct: 132 IGVSNFMPDRLMDLIVHNEIVPAVNQIETHPFYQQIESAAFVKEQGVQHQSWAPFAEGRN 191 Query: 181 NMFGNEVLASIAEKYNKSIAQVILRWLVQREVVVIPKSVRKERIVENFNIFDFELSADDI 240 NMFGNEVL SIA K+NKS+AQ++LRWLVQREVVVIPKSVRKERIVENFNIFDFEL ADD+ Sbjct: 192 NMFGNEVLTSIAGKHNKSVAQIVLRWLVQREVVVIPKSVRKERIVENFNIFDFELGADDM 251 Query: 241 EQISALDTRESLFLSYHDPKFAKMLGTLRVDL 272 E IS LDT+ESLFLSY DP AKM+G RV+L Sbjct: 252 EHISTLDTQESLFLSYRDPGVAKMMGNWRVEL 283 >C4C5F0.1 C4C5F0_9FUSO Aldo/keto reductase, diketogulonate reductase EC=1.1.1.274 Length = 283 Score = 392 bits (1006), Expect = e-107, Method: Compositional matrix adjust. Identities = 179/271 (66%), Positives = 217/271 (80%) Query: 1 MPIIGFGVYQVPDAEECENAVYEALMAGYRLIDTAAGYLNEEAVGRAIKRSGVPREELFI 60 MP++GFGVYQ+ D ECE AVY+ALMAGYRLIDTAA Y NEEAVG+A+ +SG+PR+ELFI Sbjct: 12 MPVLGFGVYQITDPAECEQAVYDALMAGYRLIDTAAAYKNEEAVGKAVIKSGIPRDELFI 71 Query: 61 TTKLWVQDAGYESXXXXXXXXXXXXXXXXXXXXXIHQPFGDYYGAWRAMEDLYREGKIKA 120 TTKLW+QDAGY+S IHQPFGD Y +WRAMEDLY EGKI+A Sbjct: 72 TTKLWIQDAGYDSTKRAFEESLKRLQTDYLDLYLIHQPFGDIYSSWRAMEDLYNEGKIRA 131 Query: 121 IGVSNFLPDRLMDLIVHNEIVPAVNQIETHPFYQQTESAAFMKEQGVQHQSWAPFAEGRN 180 IGVSNFLPDRL+DLI+HN+I PAVNQ+ETHPF QQ E+A FMKE VQ +SWAPFAEG+N Sbjct: 132 IGVSNFLPDRLLDLILHNKITPAVNQVETHPFLQQIENAEFMKENNVQIESWAPFAEGKN 191 Query: 181 NMFGNEVLASIAEKYNKSIAQVILRWLVQREVVVIPKSVRKERIVENFNIFDFELSADDI 240 N+F NE+L SIA KYNKS+AQ+ILRWL QR++VVIPKSVRKERI+ENF+IFDFELS +D+ Sbjct: 192 NIFQNEILLSIAGKYNKSVAQIILRWLTQRDIVVIPKSVRKERIIENFDIFDFELSKEDM 251 Query: 241 EQISALDTRESLFLSYHDPKFAKMLGTLRVD 271 E+I L+T +S F S+ DP+ + L ++R D Sbjct: 252 EKIVTLNTDQSSFFSHRDPEMVRFLSSIRYD 282 >B1DF75.1 B1DF75_9BACL 2,5-didehydrogluconate reductase EC=1.1.1.274 Length = 284 Score = 386 bits (991), Expect = e-105, Method: Compositional matrix adjust. Identities = 176/272 (64%), Positives = 219/272 (80%) Query: 1 MPIIGFGVYQVPDAEECENAVYEALMAGYRLIDTAAGYLNEEAVGRAIKRSGVPREELFI 60 MPI+GFGV+Q+PD CE +VY+A++AGYRLIDTAA YLNEEAVG+ IKRSGV R++LFI Sbjct: 13 MPILGFGVFQIPDPSVCEQSVYDAIIAGYRLIDTAASYLNEEAVGKGIKRSGVARKDLFI 72 Query: 61 TTKLWVQDAGYESXXXXXXXXXXXXXXXXXXXXXIHQPFGDYYGAWRAMEDLYREGKIKA 120 TTKLWVQD GYE IHQP+GD +G+WRAME+LY EGK++A Sbjct: 73 TTKLWVQDTGYERTKKAFENSLNRLQLDYLDLYLIHQPYGDVHGSWRAMEELYHEGKVRA 132 Query: 121 IGVSNFLPDRLMDLIVHNEIVPAVNQIETHPFYQQTESAAFMKEQGVQHQSWAPFAEGRN 180 IGVSNF DRL+DLI+HNE+VPAVNQ+ETHPF+QQ E+A FMKE VQ +SWAPFAEG+N Sbjct: 133 IGVSNFHEDRLIDLILHNEVVPAVNQVETHPFHQQVENATFMKEHHVQIESWAPFAEGKN 192 Query: 181 NMFGNEVLASIAEKYNKSIAQVILRWLVQREVVVIPKSVRKERIVENFNIFDFELSADDI 240 N+F NE L +IA K+NKS+AQV+LRWL QREVVVIPKSVRKERI+EN +IFDFEL+ +D+ Sbjct: 193 NLFQNEALVAIAAKHNKSVAQVVLRWLTQREVVVIPKSVRKERIIENLHIFDFELNREDM 252 Query: 241 EQISALDTRESLFLSYHDPKFAKMLGTLRVDL 272 E I++LDT++SLF S+ DP+ K LGT ++D+ Sbjct: 253 ELIASLDTKQSLFFSHRDPEMVKWLGTRKLDI 284 >B3CCU7.1 B3CCU7_9BACE Putative uncharacterized protein Length = 280 Score = 385 bits (990), Expect = e-105, Method: Compositional matrix adjust. Identities = 178/268 (66%), Positives = 213/268 (79%) Query: 1 MPIIGFGVYQVPDAEECENAVYEALMAGYRLIDTAAGYLNEEAVGRAIKRSGVPREELFI 60 MPI+GFGVYQ+ D CE VY+A+ AGYR IDTAA Y NEEAVGRAIKRSG+PRE+LFI Sbjct: 12 MPILGFGVYQIEDQALCEQCVYDAIEAGYRSIDTAAAYGNEEAVGRAIKRSGIPREDLFI 71 Query: 61 TTKLWVQDAGYESXXXXXXXXXXXXXXXXXXXXXIHQPFGDYYGAWRAMEDLYREGKIKA 120 TTKLW+ DAGY+ IHQPF DYYGAWRAME+LY+EG+I++ Sbjct: 72 TTKLWISDAGYDKAKKAFEASMKRLQLDYLDLYLIHQPFNDYYGAWRAMEELYKEGRIRS 131 Query: 121 IGVSNFLPDRLMDLIVHNEIVPAVNQIETHPFYQQTESAAFMKEQGVQHQSWAPFAEGRN 180 IGVSNF+PDRL+DLI+HNEI PAVNQ+ET+PFYQQ E+ AFM+E+GVQ +SWAPFAEG+N Sbjct: 132 IGVSNFMPDRLVDLILHNEITPAVNQVETNPFYQQIEAEAFMQEKGVQVESWAPFAEGKN 191 Query: 181 NMFGNEVLASIAEKYNKSIAQVILRWLVQREVVVIPKSVRKERIVENFNIFDFELSADDI 240 NMF NE LASIA KY KSIAQVILRWL+QR +VVIPKS+RKERI+ENFN+FDFEL +D+ Sbjct: 192 NMFQNETLASIAGKYGKSIAQVILRWLIQRNIVVIPKSIRKERIIENFNVFDFELKKEDM 251 Query: 241 EQISALDTRESLFLSYHDPKFAKMLGTL 268 ++I+ALDT S F S+ DPK + L L Sbjct: 252 KKIAALDTNTSCFFSHRDPKMVEWLSGL 279 >B2V3I3.1 B2V3I3_CLOBA Morphine 6-dehydrogenase EC=1.1.1.218 Length = 283 Score = 379 bits (972), Expect = e-103, Method: Compositional matrix adjust. Identities = 170/269 (63%), Positives = 214/269 (79%) Query: 1 MPIIGFGVYQVPDAEECENAVYEALMAGYRLIDTAAGYLNEEAVGRAIKRSGVPREELFI 60 MPI+GFGVYQ+ D +CE AVY+ALM GYR IDTA+ Y+NE+AVG AIKRSG+PR+ELFI Sbjct: 12 MPILGFGVYQIEDTIQCEQAVYDALMVGYRSIDTASVYMNEKAVGHAIKRSGIPRKELFI 71 Query: 61 TTKLWVQDAGYESXXXXXXXXXXXXXXXXXXXXXIHQPFGDYYGAWRAMEDLYREGKIKA 120 TTKLW+QDAGY + IHQPFGD YG+WRAME+LY EGKI+A Sbjct: 72 TTKLWIQDAGYNNTKKAFSQSLKHLQLDYIDLYLIHQPFGDIYGSWRAMEELYEEGKIRA 131 Query: 121 IGVSNFLPDRLMDLIVHNEIVPAVNQIETHPFYQQTESAAFMKEQGVQHQSWAPFAEGRN 180 IGVSNF PDRL+DLI+HN I+PA+NQ+ET+PF QQ ESA MK+ VQ +SWAPFAEG N Sbjct: 132 IGVSNFQPDRLVDLIIHNRIIPAINQVETNPFCQQVESAKIMKDINVQIESWAPFAEGHN 191 Query: 181 NMFGNEVLASIAEKYNKSIAQVILRWLVQREVVVIPKSVRKERIVENFNIFDFELSADDI 240 N+F N++L ++AEK+NKS+AQ+ILRWL+QR+VV IPKSV KERI+ENFNIF+FELS++D+ Sbjct: 192 NIFNNDILVTLAEKHNKSVAQIILRWLIQRDVVAIPKSVHKERIIENFNIFNFELSSNDM 251 Query: 241 EQISALDTRESLFLSYHDPKFAKMLGTLR 269 ++IS LDT+ES F S+ DPK K+ ++ Sbjct: 252 KKISILDTKESCFFSHRDPKMVKLFSEVK 280 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.137 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,499,264,436 Number of extensions: 54183236 Number of successful extensions: 146201 Number of sequences better than 1.0e-05: 3670 Number of HSP's gapped: 139910 Number of HSP's successfully gapped: 4127 Length of query: 272 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 139 Effective length of database: 1,807,701,208 Effective search space: 251270467912 Effective search space used: 251270467912 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)