BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0434.0005.p (514 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KDF3.1 B3KDF3_9BACI Replication initiation and membrane attach... 653 0.0 B1DJ00.1 B1DJ00_9BACL Replication initiation and membrane attach... 483 e-134 B7GGV0.1 B7GGV0_ANOFW Replication initiation/membrane attachment... 75 1e-11 C3BQU3.1 C3BQU3_9BACI Replication initiation and membrane attach... 74 3e-11 A7Z7I5.1 A7Z7I5_BACA2 DnaB 70 4e-10 >B3KDF3.1 B3KDF3_9BACI Replication initiation and membrane attachment family protein Length = 501 Score = 653 bits (1684), Expect = 0.0, Method: Compositional matrix adjust. Identities = 318/489 (65%), Positives = 382/489 (78%), Gaps = 15/489 (3%) Query: 1 MSNLHHFTEHHRYCVSREFGLSSVDARMLGSVYQPMVGAFAISLYRLLFEHIPAEAIGYS 60 M+N HFTE+HRYCV R+F LS +D +MLGS+YQPMVGAFA+SLYRLL H+P E +GYS Sbjct: 3 MTNFLHFTENHRYCVYRDFCLSGLDDKMLGSIYQPMVGAFAVSLYRLLVSHVPLEKVGYS 62 Query: 61 PVEQQRRLFMTLGVEPNEKGRKYIVDQASLLEAVGLLQTCRIFVAETDDYMYEYELLPPL 120 +EQQRRLF+TLG+EP+EKGR+Y+++Q S LEAVGLLQT R+FV ETDD++YEYEL PL Sbjct: 63 AIEQQRRLFLTLGLEPSEKGRRYLIEQTSKLEAVGLLQTSRMFVPETDDHVYEYELQAPL 122 Query: 121 SPADFFATQHLTLLLRDKIGKFAVLSLREQFWNREPEEWSRXXXSIGKENISLPFYDIFQ 180 SPA+FF TQHLTLLLRDK+GKFAVLSLRE+ W++EP EWS S+ KENIS+PFYDIF+ Sbjct: 123 SPAEFFNTQHLTLLLRDKVGKFAVLSLREELWSKEPLEWS--GISLNKENISVPFYDIFE 180 Query: 181 LNTHVIDYELEQALAEVATVKQPGM--RETGEPAIGYSDIILRFPRESANRVHVEKLRFD 238 LNTHVIDYELEQAL EVA ++ + GE + Y+DIILRFPRES NRV VE+LR+D Sbjct: 181 LNTHVIDYELEQALEEVAVSRKSVLPPNPQGE-GLNYADIILRFPRESRNRVFVERLRYD 239 Query: 239 HAQMGMINYVAHKYELSPQDMSRLLDEDGVFTQEGEVILDTLQYKASQHFRQDMKRQEKR 298 H QMG++NYV KY+LS QD+ RLLDEDG+FT+ GEV+LD LQ+KAS HFRQ MK QEKR Sbjct: 240 HEQMGIVNYVVRKYDLSTQDLCRLLDEDGIFTEHGEVVLDDLQHKASLHFRQGMKMQEKR 299 Query: 299 EIXXXXXXXXXXXXXXEGDDSTPPMEQPVEMEYYVEVPPQFLSKCDIHQYNMMLRNEPYT 358 +I + D+ P E V+MEYYVEVP QF SKCDIHQYNMMLRNEPYT Sbjct: 300 QITAGKVVALHQEMTADPDE--PAQEHAVQMEYYVEVPAQFQSKCDIHQYNMMLRNEPYT 357 Query: 359 RLLQTFFPGAVPTQLMDTFEKIDLNYKLSGEVINVLIHYLMTMVASAGDQRMNRNFVEAI 418 RLLQTFFPG+VP Q +D FEKIDL+YKL GEVINVLIHYLM+++A+ G+QR+NRNFVEAI Sbjct: 358 RLLQTFFPGSVPAQFLDIFEKIDLSYKLPGEVINVLIHYLMSLIAAGGEQRINRNFVEAI 417 Query: 419 ASNMLVKQVNTYEKAVRYIRDQTKVXXXXXXXXXXXXXXXRQRSYSKTGGRSGKAEIPIV 478 ASNML+KQVN+YEKAV+YIRDQ K+ R R+Y+K G K EIP+ Sbjct: 418 ASNMLLKQVNSYEKAVQYIRDQAKM------KGKQKAASQRTRTYAKGG--KAKPEIPVA 469 Query: 479 LDDGSAGTV 487 L+ A V Sbjct: 470 LETDQAEAV 478 >B1DJ00.1 B1DJ00_9BACL Replication initiation and membrane attachment family protein Length = 506 Score = 483 bits (1243), Expect = e-134, Method: Compositional matrix adjust. Identities = 251/492 (51%), Positives = 331/492 (67%), Gaps = 13/492 (2%) Query: 1 MSNLHHFTEHHRYCVSREFGLSSVDARMLGSVYQPMVGAFAISLYRLLFEHIPAEAIGYS 60 + NL +TEHHRY + R+F LSS+D +ML +YQPM+GAFAI+LY+ L+ I GYS Sbjct: 3 IGNLLQYTEHHRYYIFRDFSLSSLDYKMLSLIYQPMLGAFAIALYQQLYHGIAEGTSGYS 62 Query: 61 PVEQQRRLFMTLGVEPNEKGRKYIVDQASLLEAVGLLQTCRIFVAETDDYMYEYELLPPL 120 +E QR+LF+ LG++ NE+GRKY++DQAS LEAVGLLQT R+ + + D MYEYEL PL Sbjct: 63 AIESQRKLFLGLGLDMNERGRKYLIDQASRLEAVGLLQTSRVGLPDQADVMYEYELSQPL 122 Query: 121 SPADFFATQHLTLLLRDKIGKFAVLSLREQFWNREPEEWSRXXXSIGKENISLPFYDIFQ 180 SP +FF QHLTLLLRDKIGK+AV+SLRE F +EP+E ++ + KENIS+PFY++FQ Sbjct: 123 SPGEFFRNQHLTLLLRDKIGKYAVISLRESFGAQEPDELAQ--KQLTKENISVPFYELFQ 180 Query: 181 LNTHVIDYELEQALAEVATVKQPGMRETGEPA-IGYSDIILRFPRESANRVHVEKLRFDH 239 LNT +D ELEQAL EVA +Q R E A I Y +IILRFPR SANR +VE++R + Sbjct: 181 LNTQSVDQELEQALTEVAPTRQAAPRAQLETAGIPYGEIILRFPRNSANRSYVERMRGNS 240 Query: 240 AQMGMINYVAHKYELSPQDMSRLLDEDGVFTQEGEVILDTLQYKASQHFRQDMKRQEKRE 299 Q+ +NYVA+KY LS D+ RLLDEDG+F GE+I+D +Q +A+Q +RQD KR+ +R+ Sbjct: 241 EQLAQVNYVAYKYNLSVADICRLLDEDGIFASNGELIVDEIQLRANQLYRQDKKREGERQ 300 Query: 300 IXXXXXXXXXXXXXXEGDDSTPPMEQPVEMEYYVEVPPQFLSKCDIHQYNMMLRNEPYTR 359 + +E V+ EYY+ VP Q +CD+ QYNM++RNEP+TR Sbjct: 301 ---RTLARVQAAAVQPNETEEELVEFGVQEEYYMLVPSQLAGRCDVQQYNMLMRNEPHTR 357 Query: 360 LLQTFFPGAVPTQLMDTFEKIDLNYKLSGEVINVLIHYLMTMVASAGDQRMNRNFVEAIA 419 LQ FFPGAVP + +FE+IDLNYKL VIN+LIHY++ M + QR+ + FVEA+ Sbjct: 358 FLQRFFPGAVPDWIERSFERIDLNYKLPAPVINILIHYVIGMNDA---QRVTKTFVEAVV 414 Query: 420 SNMLVKQVNTYEKAVRYIRDQTKVXXXXXXXXXXXXX---XXRQRSY-SKTGGRSGKAEI 475 SNMLVKQV+T+EKAV Y+R+Q +V R S S GG S K I Sbjct: 415 SNMLVKQVDTFEKAVLYVREQAEVEADKERRNARAAAGLPGTRGTSRNSSRGGTSRKPSI 474 Query: 476 PIVLDDGSAGTV 487 PIV D+ + T Sbjct: 475 PIVQDEEAGDTA 486 >B7GGV0.1 B7GGV0_ANOFW Replication initiation/membrane attachment protein Length = 471 Score = 75.5 bits (184), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 113/450 (25%), Positives = 186/450 (41%), Gaps = 77/450 (17%) Query: 12 RYCVSREFGLSSVDARMLGSVYQPMVGAFAISLYRLLFEHIPAEAIGYSPVEQQRRLFMT 71 RY V LS +D ++L +YQP++GA A SLY L +S +EQ R Sbjct: 19 RYIVHANGVLSELDRKVLTLLYQPLIGAQAYSLYMTL----------WSELEQAR----- 63 Query: 72 LGVEPNEKGRKYIVDQASL---------LEAVGLLQTCRIFVAETDDYMYEYELLPPLSP 122 L E + Q SL LE +GLL T D+ ++ YEL PL+P Sbjct: 64 LWSEETTHHSLMAIMQCSLPTIYAERLKLEGIGLLNTYMKRTGADDERVFIYELRLPLTP 123 Query: 123 ADFFATQHLTLLLRDKIGKFAVLSLREQFWNREPEEWSRXXXSIGKENISLPFYDIF-QL 181 FF L + L D++GK L++ F +R + S ++ F+D+F + Sbjct: 124 EQFFTDGVLNVFLYDRVGKTKFQKLKKFFSDRAIDHTSFV-------PVTRSFHDVFSSI 176 Query: 182 NTHVIDYELEQALAEVATVKQPGMRETGEPAIGYS--DIILRFPRESANRVH--VEKLRF 237 + ++L + T+ Q G + G+ Y+ D I F A H V + Sbjct: 177 QPEKMIHKLSDEAKQEVTL-QDGHQYIGKEQAMYTMRDDIFDFDAFYAGLSHHLVPRRAI 235 Query: 238 DHAQMGMINYVAHKYELSPQDMSRLLDEDGVFTQEGEVILDTLQYKASQHFRQDMKRQEK 297 I +A Y + P DM +++ GV ++ ++ L+ KA+Q + Q Sbjct: 236 TEKVKEAIKKLAFLYGIGPLDMQKIV--MGVIDPAEQIDIEALR-KAAQEWYQ------- 285 Query: 298 REIXXXXXXXXXXXXXXEGDDSTPPME---QPVEMEYYVEVPPQFLSKCDIHQYNM-MLR 353 E ++ P + QP+ V P+ + +Y M L Sbjct: 286 ----------------FERGEALPRLYDRVQPLAERTMAHVEPK-----NEEEYLMKQLE 324 Query: 354 NEPYTRLLQTFFPGAVPTQL-MDTFEKIDLNYKLSGEVINVLIHYLMTMVASAGDQRMNR 412 +LL F GA P + E++ KL V+NVLI+Y+M D ++ + Sbjct: 325 TVSPRQLLMDFSGGAEPALADLQLIEEMMFKQKLPPGVMNVLIYYVMLRT----DMKLPK 380 Query: 413 NFVEAIASNMLVKQVNTYEKAVRYIRDQTK 442 +VE IAS+ K+V T ++A+ + + + Sbjct: 381 KYVEKIASSWARKKVRTVKQAMELAKKEAR 410 >C3BQU3.1 C3BQU3_9BACI Replication initiation and membrane attachment protein Length = 468 Score = 74.3 bits (181), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 107/449 (23%), Positives = 185/449 (41%), Gaps = 82/449 (18%) Query: 12 RYCVSREFGLSSVDARMLGSVYQPMVGAFAISLYRLLFEHIPAEAIGYSPVEQQRRLFMT 71 RY VS + L S D ++L +YQP+VG+ A SLY L+ + + + + L +T Sbjct: 14 RYKVSAKGLLHSYDRKVLTMLYQPLVGSKAFSLYMTLWGELEQDRV-FGKENTHHSLMVT 72 Query: 72 LGVEPNEKGRKYIVDQASLLEAVGLLQTCRIFVAETDDYMYEYELLPPLSPADFFATQHL 131 + ++ E + ++ LEA+GLL E D M+ YEL PPLSP FF L Sbjct: 73 MQMQLPE-----VYEERMKLEAIGLLNVY--IKKEKDIRMFIYELQPPLSPKQFFDDIVL 125 Query: 132 TLLLRDKIGKFAVLSLREQFWNREPEEWSRXXXSIGKENISLPFYDIF-QLNTHVIDYEL 190 ++ L +++ + +++ F E + S EN++ F D+F N ++ Sbjct: 126 SIFLYNRLSRAKYNQVKQYFLEEEFDFASY-------ENVTHSFNDVFGSFNPGQFEHAQ 178 Query: 191 EQALAEVATVKQPGMRETGEPAI------------GYSDIILRFPRESANRVHVEKLRFD 238 E+ + V PG + P + G S ++ R R V L + Sbjct: 179 EELRIPSSAV-MPGNEKGTAPQLWNDFFDFSLFVEGLSTLVPRKAITDQVRECVVTLSYV 237 Query: 239 HA--QMGMINYV-AHKYELSPQDMSRLLDEDGVFTQ-EGEVILDTLQYKASQHFRQDMKR 294 + + M N V EL DM RL + Q E L L + H + MK Sbjct: 238 YGVDVLSMQNIVLGAMTELQTIDMERLRKGARDWYQFENGQALPVLSERVQPHAARTMKE 297 Query: 295 QEKREIXXXXXXXXXXXXXXEGDDSTPPMEQPVEMEYYVEVPPQFLSKCDIHQYNMMLRN 354 +E P ++ + ++ E+ P+ Sbjct: 298 KE------------------------PSTQEEMLIKQLEEISPK---------------- 317 Query: 355 EPYTRLLQTFFPGAVPTQL-MDTFEKIDLNYKLSGEVINVLIHYLMTMVASAGDQRMNRN 413 +LL+ GA T+ + E++ +N KL+ V+NVLI+Y+M D ++ + Sbjct: 318 ----QLLKEISGGAEATKADLQIVEEVMMNQKLTPGVVNVLIYYVML----RSDMKLAKT 369 Query: 414 FVEAIASNMLVKQVNTYEKAVRYIRDQTK 442 +VE IA + K+V T +A+ +++ + Sbjct: 370 YVEKIAGHWARKKVGTVSEAMALAKEENR 398 >A7Z7I5.1 A7Z7I5_BACA2 DnaB Length = 469 Score = 70.5 bits (171), Expect = 4e-10, Method: Compositional matrix adjust. Identities = 60/198 (30%), Positives = 93/198 (46%), Gaps = 23/198 (11%) Query: 13 YCVSREFGLSSVDARMLGSVYQPMVGAFAISLYRLLFEHIPAEAIGYSPVEQQRRLFMTL 72 Y V L VD ++L +YQP++G+FA SLY L+ + + + E R M + Sbjct: 14 YTVKSRSLLQDVDRQVLTHLYQPLIGSFAFSLYMTLWGELEQNRLWGA--ESTHRQLMGM 71 Query: 73 GVEPNEKGRKYIVDQASLLEAVGLLQTCRIFVAETDDYMYEYELLPPLSPADFFATQHLT 132 + K I + LE +GLL+ I +E + M+ YELLPPL P DFF L Sbjct: 72 ----MQSNLKKIHHEQGKLEGIGLLKVY-IRESEQKERMFIYELLPPLRPNDFFEDGMLN 126 Query: 133 LLLRDKIGKFAVLSLREQFWNREPEEWSRXXXSIGKENISLPF---YDIFQLNTHVIDYE 189 + L +++GK LR QF ++ G I+ PF + FQ + + + Sbjct: 127 VFLYNRVGKTKYQQLR-QF-------YTHPSVGAGAREITRPFNHAFASFQPSEWKMTPD 178 Query: 190 LEQALA-----EVATVKQ 202 +E+A+ E A+V Q Sbjct: 179 MEEAMKLPEDYETASVGQ 196 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.136 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,675,196,722 Number of extensions: 102721422 Number of successful extensions: 211868 Number of sequences better than 1.0e-05: 121 Number of HSP's gapped: 211860 Number of HSP's successfully gapped: 121 Length of query: 514 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 374 Effective length of database: 1,741,754,103 Effective search space: 651416034522 Effective search space used: 651416034522 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)