BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0434.0001.p (424 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KEH3.1 B3KEH3_9BACI DEAD/DEAH box helicase domain protein 497 e-139 B1D7A8.1 B1D7A8_9BACL DEAD/DEAH box helicase domain protein 331 1e-88 C0ZEQ4.1 C0ZEQ4_BREBN Putative ATP-dependent RNA helicase 293 3e-77 A6TX49.1 A6TX49_ALKMQ DEAD/DEAH box helicase domain protein 288 5e-76 B1KS82.1 B1KS82_CLOBM ATP-dependent RNA helicase, DEAD/DEAH box ... 288 8e-76 >B3KEH3.1 B3KEH3_9BACI DEAD/DEAH box helicase domain protein Length = 448 Score = 497 bits (1280), Expect = e-139, Method: Compositional matrix adjust. Identities = 253/381 (66%), Positives = 304/381 (79%) Query: 1 MNKASFAAIGIQEDFVARLSEFGIQDPSPVQKETIPLLLEGRDVLAASQTGTGKTLAYLL 60 M +F +GI ED +RL+EFGI PSPVQ E IP +LEG+DVLA SQTGTGKTLAYLL Sbjct: 1 MTGTTFHTLGIAEDLSSRLAEFGIATPSPVQAEAIPPILEGKDVLATSQTGTGKTLAYLL 60 Query: 61 PLLQGINPEQKVVQKLVLAPTQELAMQILREAERYGAHRGIKVLGLIGGAAIKRQIDKLR 120 P+LQGI+PE K QKL+LAPTQELA+QI+RE+ERYG RGI VLGLIGGAA KRQI+KLR Sbjct: 61 PVLQGIDPEIKGAQKLILAPTQELAVQIVRESERYGEARGIGVLGLIGGAAAKRQIEKLR 120 Query: 121 EHPQLVVGTPGRIRELIGLRKLKMHEVTTIVIDEADQMFQLSGAGEVAKIVSSALRTRQL 180 HPQL+VGTPGR+RELI +RKLKMH+VTTIV+DE DQ+F L GAG+V +I+ SALR RQL Sbjct: 121 LHPQLIVGTPGRVRELIEIRKLKMHQVTTIVVDEVDQVFNLGGAGDVDRILRSALRDRQL 180 Query: 181 VMLSATIGPETKALAAREMKNPAEIGIDPGIMTAQSLEHHYVVTEERNKIDMLRRVIRHY 240 V LSAT+ PET L +EM P EIGIDP T +LEH+Y V+EER+K+DMLRRV+RHY Sbjct: 181 VFLSATVSPETAELVKKEMDQPVEIGIDPERRTPSALEHYYFVSEERDKLDMLRRVVRHY 240 Query: 241 NPERAIVFVNATDDIAEVTAKLNYLGLTAAALYGDADKVTRSNVLARFRDGRFRVLVASD 300 NP+RAIVFVNAT+D+AEV AKLNYLGL AAALYGDADK+TRS VL FR+GR ++LVASD Sbjct: 241 NPDRAIVFVNATEDLAEVEAKLNYLGLNAAALYGDADKMTRSRVLNAFREGRTKLLVASD 300 Query: 301 VAARGLDIENLTLVVSFDPAFDSEHYVHXXXXXXXXXXXXLSVTIVTEQQTFIMRKFAKE 360 VAARGLDIE L +V+++DP D+EHY H +++T+VT++QTFIMRKF +E Sbjct: 301 VAARGLDIEGLGMVINYDPPIDAEHYTHRAGRTGRMGRSGMAITLVTDRQTFIMRKFNRE 360 Query: 361 LDIRLEEREMVGGKALTAEEA 381 L I +EER + GGK T A Sbjct: 361 LGISIEERALYGGKVRTPRSA 381 >B1D7A8.1 B1D7A8_9BACL DEAD/DEAH box helicase domain protein Length = 472 Score = 331 bits (848), Expect = 1e-88, Method: Compositional matrix adjust. Identities = 178/374 (47%), Positives = 246/374 (65%) Query: 1 MNKASFAAIGIQEDFVARLSEFGIQDPSPVQKETIPLLLEGRDVLAASQTGTGKTLAYLL 60 M++ ++ +GI + +L I+ PS +Q E IP LLEGRDV A SQTG+GKTLAYLL Sbjct: 1 MSQKTWNDLGINKALGDKLEANNIKVPSAIQAEAIPTLLEGRDVSARSQTGSGKTLAYLL 60 Query: 61 PLLQGINPEQKVVQKLVLAPTQELAMQILREAERYGAHRGIKVLGLIGGAAIKRQIDKLR 120 P+LQ + P +Q ++LAPTQELAMQI+R AE Y G++V LIGGAA+KRQI+KL+ Sbjct: 61 PMLQQLKPSSGAIQAIILAPTQELAMQIVRVAEGYAEPLGLRVQQLIGGAAMKRQIEKLK 120 Query: 121 EHPQLVVGTPGRIRELIGLRKLKMHEVTTIVIDEADQMFQLSGAGEVAKIVSSALRTRQL 180 PQ+V+GTPGRI EL+ LRKLK+ +V IVIDEADQ+F+L EV I+ + + RQL Sbjct: 121 LRPQIVIGTPGRIHELLKLRKLKLSDVRHIVIDEADQVFELGSTREVETILFAMSQERQL 180 Query: 181 VMLSATIGPETKALAAREMKNPAEIGIDPGIMTAQSLEHHYVVTEERNKIDMLRRVIRHY 240 SAT R MK+ I I P ++++ + YVV ++R+K D RR++R Sbjct: 181 AFFSATYPDVMARFEGRWMKDTVRIQIAPEQRVSETINNFYVVCDKRDKPDAARRIVRML 240 Query: 241 NPERAIVFVNATDDIAEVTAKLNYLGLTAAALYGDADKVTRSNVLARFRDGRFRVLVASD 300 PE A++F+N T++IA AKL+Y G + LYGD+DK R+ LARFR+G+ ++L+A+D Sbjct: 241 KPESALLFLNVTENIANWEAKLSYEGFSVETLYGDSDKQRRAATLARFREGKTQLLLATD 300 Query: 301 VAARGLDIENLTLVVSFDPAFDSEHYVHXXXXXXXXXXXXLSVTIVTEQQTFIMRKFAKE 360 VAARGLDIE L LV++ DP D++HYVH ++++T Q+ FIM KF K Sbjct: 301 VAARGLDIEGLQLVINLDPPIDADHYVHRAGRTGRMGRKGTVISLITPQERFIMDKFRKS 360 Query: 361 LDIRLEEREMVGGK 374 L I L E+ M G+ Sbjct: 361 LRIELPEKAMYKGE 374 >C0ZEQ4.1 C0ZEQ4_BREBN Putative ATP-dependent RNA helicase Length = 414 Score = 293 bits (749), Expect = 3e-77, Method: Compositional matrix adjust. Identities = 162/376 (43%), Positives = 229/376 (60%), Gaps = 15/376 (3%) Query: 5 SFAAIGIQEDFVARLSEFGIQDPSPVQKETIPLLLEGRDVLAASQTGTGKTLAYLLPLLQ 64 +FA + I + L E ++ P+PVQ E IPL++ GRD L S TGTGKT AYLLP+L Sbjct: 4 TFADLQISAPLLTILRERKMETPTPVQSEAIPLIMAGRDALVESPTGTGKTFAYLLPILS 63 Query: 65 GINPEQKVVQKLVLAPTQELAMQILREAERYGAHRGIKVLGLIGGAAIKRQIDKLREHPQ 124 +N EQK VQ +VLAPT EL +QI REAE + V+ +IGG +KRQI+KL++ P Sbjct: 64 KVNMEQKDVQAIVLAPTHELVVQIAREAESLLPGKDTAVMSIIGGVDVKRQIEKLKKKPV 123 Query: 125 LVVGTPGRIRELIGLRKLKMHEVTTIVIDEADQMFQLSGAGEVAKIVSSALRTRQLVMLS 184 L+V TPGR+ ELI RKLK+HEV T+V+DEAD+M V +++ LR Q ++ S Sbjct: 124 LIVATPGRLLELIEQRKLKVHEVKTVVVDEADRMLDAGFGKPVQEVMKKTLRDTQRLLFS 183 Query: 185 ATIGPETKALAAREMKNPAEIGI-DPGIMTAQSLE------HHYVVTEERNKIDMLRRVI 237 ATI A E+ + AEI D ++ A + E H Y+VT+ R K+D LRR++ Sbjct: 184 ATI--------AEEVVHTAEIFTKDAAVIRAAAPEGAAGVAHMYLVTDPRKKVDTLRRLL 235 Query: 238 RHYNPERAIVFVNATDDIAEVTAKLNYLGLTAAALYGDADKVTRSNVLARFRDGRFRVLV 297 R N +IVFVN + + E+ +KLNY L L+ DA K R+ L +FRDG F VL+ Sbjct: 236 RLVNVRSSIVFVNQIEKVDEIVSKLNYHNLPCRLLHRDATKEERARTLQQFRDGVFPVLI 295 Query: 298 ASDVAARGLDIENLTLVVSFDPAFDSEHYVHXXXXXXXXXXXXLSVTIVTEQQTFIMRKF 357 +DV+ARG+DI + +V +DPA D++ Y+H L +IVT Q+ FI++KF Sbjct: 296 TTDVSARGIDIPGVECIVHYDPASDADTYIHRSGRTGRMGRAGLVFSIVTPQEQFIIQKF 355 Query: 358 AKELDIRLEEREMVGG 373 +K+ + + E+ M G Sbjct: 356 SKQTGLPIAEKFMTHG 371 >A6TX49.1 A6TX49_ALKMQ DEAD/DEAH box helicase domain protein Length = 387 Score = 288 bits (738), Expect = 5e-76, Method: Compositional matrix adjust. Identities = 152/357 (42%), Positives = 220/357 (61%), Gaps = 4/357 (1%) Query: 23 GIQDPSPVQKETIPLLLEGRDVLAASQTGTGKTLAYLLPLLQGINPEQKVVQKLVLAPTQ 82 G P+P+Q+E IPL+LEG+D++A S TGTGKTLAYL+P+L I+PE K VQ ++LAP+ Sbjct: 29 GFTAPTPIQEEAIPLILEGKDLIAESPTGTGKTLAYLIPILHRIDPESKAVQAVILAPSH 88 Query: 83 ELAMQILREAERYGAHRGIKVLGLIGGAAIKRQIDKLREHPQLVVGTPGRIRELIGLRKL 142 ELAMQI + E++ I LIGGA IKRQI+ L++ PQ++V T GR+ E+I L+K+ Sbjct: 89 ELAMQIHQTIEKWTKDNNISSEPLIGGANIKRQIENLKKRPQIIVATTGRLLEVIKLKKI 148 Query: 143 KMHEVTTIVIDEADQMFQLSGAGEVAKIVSSALRTRQLVMLSATIGPETKALAAREMKNP 202 KMHEV TIV+DE D + A + I+ + L+ RQ+V SATI T+ + MK P Sbjct: 149 KMHEVKTIVVDEFDILIAEEHAENLKHIIKTTLKERQIVCFSATISENTEQIGMELMKEP 208 Query: 203 AEIGIDPG---IMTAQSLEHHYVVTEERNKIDMLRRVIRHYNPERAIVFVNATDDIAEVT 259 I I G + T +++H Y++ E R K ++LR++IR N +A+VF N I E+ Sbjct: 209 KIIQIQKGQSSLSTLSNIQHTYIICEHREKFEVLRKIIRSGNV-KALVFTNDVRKIEEIQ 267 Query: 260 AKLNYLGLTAAALYGDADKVTRSNVLARFRDGRFRVLVASDVAARGLDIENLTLVVSFDP 319 KL+Y G+ L G ++K R L FR G+F +LVA+DVAARGLDIE LT V+++D Sbjct: 268 VKLSYNGIDLGVLMGGSNKTQRKEALNGFRMGKFPLLVATDVAARGLDIEGLTHVINWDV 327 Query: 320 AFDSEHYVHXXXXXXXXXXXXLSVTIVTEQQTFIMRKFAKELDIRLEEREMVGGKAL 376 ++ Y H V+IV +++ + RK EL+I E+ M G+ + Sbjct: 328 PLTADQYTHRSGRTGRMGALGTVVSIVNKREESMFRKITNELEISATEKMMYKGEMI 384 >B1KS82.1 B1KS82_CLOBM ATP-dependent RNA helicase, DEAD/DEAH box family Length = 421 Score = 288 bits (737), Expect = 8e-76, Method: Compositional matrix adjust. Identities = 143/380 (37%), Positives = 236/380 (62%), Gaps = 2/380 (0%) Query: 5 SFAAIGIQEDFVARLSEFGIQDPSPVQKETIPLLLEGRDVLAASQTGTGKTLAYLLPLLQ 64 SF + + V L + I P+ +Q E IPL +E +D++ S+TGTGKTLAYLLP+ + Sbjct: 4 SFDTLNLNPKLVQGLKKLNINIPTEIQAEAIPLAMENKDIIGQSETGTGKTLAYLLPIFE 63 Query: 65 GINPEQKVVQKLVLAPTQELAMQILREAERYGAHRGI--KVLGLIGGAAIKRQIDKLREH 122 I E++ +Q ++LAPT ELAMQI E + ++ I K + +IG A IKRQI+KL+E Sbjct: 64 KITTEKREMQAIILAPTHELAMQINNEIKNISSNSNIDVKSMAIIGEANIKRQIEKLKEK 123 Query: 123 PQLVVGTPGRIRELIGLRKLKMHEVTTIVIDEADQMFQLSGAGEVAKIVSSALRTRQLVM 182 P ++VG+PGRI ELI +K+ H V TIVIDE D++ + V +++ + LR RQL++ Sbjct: 124 PHIIVGSPGRILELIKKKKITAHTVKTIVIDEGDKLLDKNNLKVVKEVIKTTLRERQLML 183 Query: 183 LSATIGPETKALAAREMKNPAEIGIDPGIMTAQSLEHHYVVTEERNKIDMLRRVIRHYNP 242 SATI T +A MK+ + + ++++H Y+ E R +I++LR++I NP Sbjct: 184 FSATITESTLNIAKDLMKDSEVVKVKEKNTVNENIKHLYITGEHRERIEILRKLIAATNP 243 Query: 243 ERAIVFVNATDDIAEVTAKLNYLGLTAAALYGDADKVTRSNVLARFRDGRFRVLVASDVA 302 +RAIVF+N ++I T KL Y + A +YG A+K R L FR G+ ++LV+SD++ Sbjct: 244 KRAIVFINRNEEIELTTLKLQYHKIKAYGIYGAAEKEQRKKALEDFRSGKVQILVSSDLS 303 Query: 303 ARGLDIENLTLVVSFDPAFDSEHYVHXXXXXXXXXXXXLSVTIVTEQQTFIMRKFAKELD 362 ARGLD++++T + + D + + Y+H ++++I+TE++ ++RK+ KE + Sbjct: 304 ARGLDVKDVTHIFNLDLPENPKEYLHRVGRTGRASKEGIAISIITEKEKALIRKYEKEFN 363 Query: 363 IRLEEREMVGGKALTAEEAR 382 I +EE+++ G ++++ R Sbjct: 364 INIEEKKIYKGTLISSKGER 383 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.136 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,082,353,701 Number of extensions: 77357856 Number of successful extensions: 289143 Number of sequences better than 1.0e-05: 10292 Number of HSP's gapped: 270939 Number of HSP's successfully gapped: 10466 Length of query: 424 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 286 Effective length of database: 1,760,596,133 Effective search space: 503530494038 Effective search space used: 503530494038 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)