BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0427.0026.p (295 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K9B6.1 B3K9B6_9BACI S-layer domain protein 89 7e-16 B1DGS5.1 B1DGS5_9BACL S-layer domain protein 83 4e-14 B1D624.1 B1D624_9BACL S-layer domain protein 77 3e-12 B1D8H3.1 B1D8H3_9BACL NEAr transporter 73 4e-11 B1HR66.1 B1HR66_LYSSC S-layer-like region 68 1e-09 >B3K9B6.1 B3K9B6_9BACI S-layer domain protein Length = 349 Score = 88.6 bits (218), Expect = 7e-16, Method: Compositional matrix adjust. Identities = 73/290 (25%), Positives = 131/290 (45%), Gaps = 36/290 (12%) Query: 15 CVLGISLVIHANIALAEQSSKFKDVPDHFWGQEYIERAIQHKIVEGYPDGTFKPDAKVSQ 74 +L +SL + A A A F+D+ +H W + IE + IV GY +G F P+ V++ Sbjct: 11 ILLAVSLFVGAGSAFA--GVTFRDIDNH-WAKTTIEWGVDKGIVNGYANGMFMPNQNVTE 67 Query: 75 SEFIAMLIRSYQPSDFSSSQKSQNWAEPYLSYIH-----KLGWTELQPSEQAALNRGNVA 129 +EFI ML+ D + ++ W++ Y ++H G+ + Q + R +VA Sbjct: 68 AEFIRMLVVGITGKDLQENYLTELWSDKYYHFLHFKNYPVHGFADKTKRSQ-YITRAHVA 126 Query: 130 RYLASAAGKNFTVDDSIQYLLDIGIAKG--KTERSLQGFNKNEPVTRTEALAFIERLKYR 187 ++SA G N+ + +IQY+L A+G K E +++G+ + +TR E L I+ L R Sbjct: 127 ELISSADGVNYGGNQAIQYVLGKKYAQGRIKGENTIEGYVGSGTITRAEVLQLIKNLTDR 186 Query: 188 -----FDELKS------IPKTTEKYKSDLAQKDVYTIPEQNISIQFPQQWKNKYEVVTDK 236 +D + +PK + D+ + ++Y + Q + FP+ + Y + D Sbjct: 187 GMSNLYDRPTAPSAEAMLPKLPTPW--DVYRDEMYLVIRQKV---FPKY--SGYRIYDDG 239 Query: 237 ENYLLFLEVLEEPILPHLNDRQDYLHMACFELSVITFGATDCSANSGKRQ 286 N ++ + DY FE ++ F S + K Q Sbjct: 240 SNKIVMTSTKQ-------TGETDYAVSVQFEPQIMQFSGIALSNAADKTQ 282 >B1DGS5.1 B1DGS5_9BACL S-layer domain protein Length = 343 Score = 82.8 bits (203), Expect = 4e-14, Method: Compositional matrix adjust. Identities = 36/89 (40%), Positives = 54/89 (60%), Gaps = 8/89 (8%) Query: 32 QSSKFKDVPDHFWGQEYIERAIQHKIVEGYPDGTFKPDAKVSQSEFIAMLIRSYQPS--- 88 S FKDVP+ FW + IERA IV GYPDG+FKP+ V+++EF+AML+R+ + + Sbjct: 160 SSISFKDVPEGFWAKSAIERAASMGIVTGYPDGSFKPNLAVTRAEFVAMLVRALKGNAGA 219 Query: 89 -----DFSSSQKSQNWAEPYLSYIHKLGW 112 +F+ + WA+PY++ GW Sbjct: 220 IQSELNFADNGSIGGWAKPYIATAASEGW 248 >B1D624.1 B1D624_9BACL S-layer domain protein Length = 348 Score = 76.6 bits (187), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 35/87 (40%), Positives = 51/87 (58%), Gaps = 7/87 (8%) Query: 33 SSKFKDVPDHFWGQEYIERAIQHKIVEGYPDGTFKPDAKVSQSEFIAMLIRSY------- 85 S +F DV FW ++ I RA Q V GYPDGTFKP +V+++EF+ ML R+ Sbjct: 168 SREFTDVAAGFWAKDAITRAAQMGFVNGYPDGTFKPLKQVTRAEFVTMLARALELPVAEQ 227 Query: 86 QPSDFSSSQKSQNWAEPYLSYIHKLGW 112 Q +DF +++ NWA+P ++ GW Sbjct: 228 QGTDFKDNERIGNWAKPSIASGVAAGW 254 >B1D8H3.1 B1D8H3_9BACL NEAr transporter Length = 1149 Score = 72.8 bits (177), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 56/180 (31%), Positives = 83/180 (46%), Gaps = 39/180 (21%) Query: 35 KFKDVPDHFWGQEYIERAIQHKIVEGYPDGTFKPDAKVSQSEFIAMLIRSYQ------PS 88 KF D+ H W + IE A++ IV G+ DG+F+PD KVS+ EF M+ R+ Q + Sbjct: 971 KFSDINTH-WAKANIEAAVKLGIVNGFTDGSFRPDNKVSRGEFAVMISRALQLEGEDTET 1029 Query: 89 DFSSSQKSQNWAEPYLSYIHKLGWTE------LQPSEQAALNRGNVARYLASAAGKNFTV 142 F NWA+ +++ G + + SEQ L+R +A +A AA N + Sbjct: 1030 VFQDGSTIPNWAKSHIARAVAAGLMQGFADDTFRISEQ--LSRAQLAVIIARAA--NLKL 1085 Query: 143 DDS------------------IQYLLDIGIAKGKTERSLQGFNKNEPVTRTEALAFIERL 184 DD+ + +D G+ +GK R F NE TR EA+ I RL Sbjct: 1086 DDNGSASFSDSKDIPAWASKEVAAAVDAGLIQGKDNR----FAPNETATRAEAVTMIIRL 1141 >B1HR66.1 B1HR66_LYSSC S-layer-like region Length = 342 Score = 67.8 bits (164), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 59/197 (29%), Positives = 96/197 (48%), Gaps = 23/197 (11%) Query: 9 MRKLLSCVLGISLVIHANIALAEQSSKFKDVPDHFWGQEYIERAIQHKIVEGYPDGTFKP 68 M+KL+ +L L++ + A Q++ FKDV + + E I A ++ IV G+ DGTFKP Sbjct: 1 MKKLIGILLFSILIVGS--ASNVQAAGFKDVSEDHYAYEAILWAEEYDIVNGFADGTFKP 58 Query: 69 DAKVSQSEFIAMLIRSYQ---PSDFSSSQK-SQNWAEPYLSYIHKLGW-------TELQP 117 +A +++ + +L Y+ P D Q S W++ + + + G L+ Sbjct: 59 NATITEQQLAKLLAIFYELESPIDELKKQTPSAMWSDEFYNQLASYGVPLNGYFDNRLRA 118 Query: 118 SEQAALNRGNVARYLASAAGKNFTVDDSIQYLLDIGIAKGKT----ERSLQGFNKNEPVT 173 A + RG VA+ + A +D SIQ+LLD I+ G+ +R+LQ F VT Sbjct: 119 ---ATVKRGVVAQAITHLASGKKGLDSSIQFLLDYYISSGQNPQYEDRNLQKF---YGVT 172 Query: 174 RTEALAFIERLKYRFDE 190 A + + YR DE Sbjct: 173 NNMTRAQVVTMLYRMDE 189 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.133 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,752,540,073 Number of extensions: 71576614 Number of successful extensions: 174466 Number of sequences better than 1.0e-05: 136 Number of HSP's gapped: 174597 Number of HSP's successfully gapped: 136 Length of query: 295 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 161 Effective length of database: 1,798,280,193 Effective search space: 289523111073 Effective search space used: 289523111073 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)