BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0427.0008.p (411 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KFE1.1 B3KFE1_9BACI Cyanate transporter 499 e-139 C0Z4P7.1 C0Z4P7_BREBN Conserved hypothetical membrane protein 498 e-139 A7GF62.1 A7GF62_CLOBL Transporter, major facilitator family 447 e-124 B1KVD2.1 B1KVD2_CLOBM Transporter, major facilitator family 447 e-123 B1IG39.1 B1IG39_CLOBK Transporter, major facilitator family 444 e-123 >B3KFE1.1 B3KFE1_9BACI Cyanate transporter Length = 412 Score = 499 bits (1286), Expect = e-139, Method: Compositional matrix adjust. Identities = 267/377 (70%), Positives = 306/377 (81%), Gaps = 3/377 (0%) Query: 35 IAANLRAPLTSVGPLVGLIRENVHISNTLAGLITTVPLIAFALLSPFVPKLGRRYGVERI 94 IAANLR PLTSVGPLV I+++VHISNTLAGLITTVPL+AFALLSP VPKLGRRYGVERI Sbjct: 22 IAANLRTPLTSVGPLVSFIKDDVHISNTLAGLITTVPLLAFALLSPLVPKLGRRYGVERI 81 Query: 95 MLISLILLVIGIAVRSLSGAVTLFVGTAVLGFAITICNVLLPSLIKREFPLQMGTMTGVY 154 +LI+LI L +GI +RSLSGAV LF+GTAVLGFAI ICNVLLPS+IKR+FP ++G+MTGVY Sbjct: 82 ILIALIFLTVGIVIRSLSGAVNLFIGTAVLGFAIAICNVLLPSIIKRDFPDKIGSMTGVY 141 Query: 155 SVSMNLSGAIASGISVPLAVGAGLNWQGALGVWGILSVISILFWAPQLK-XXXXXXXXXX 213 S+SMNL GAIASGISVPLAV AGL WQGALG+WGILS +SIL W PQL+ Sbjct: 142 SISMNLCGAIASGISVPLAVNAGLKWQGALGIWGILSFVSILCWLPQLRNQANQTAKTSQ 201 Query: 214 XXXGNDHHVNIWRSPLAWQVTLFMGLQSTIFYVLVAWLPEILKDQGISSSQSGWFLSIMI 273 ND VN+WRSPLAWQVTLFMG+QS +FYVL+AWLPEILK QGI S+QSGWFLSIM Sbjct: 202 KMVSND--VNVWRSPLAWQVTLFMGIQSMVFYVLIAWLPEILKQQGIDSNQSGWFLSIMQ 259 Query: 274 IASLPLSFIVPVIAGRMRNQRXXXXXXXXXXXXXXXXXXYGSIHLVLLWTIILGIGSGFA 333 +A LP +FIVPVIAGRM +QR YGS +++LLW IILGIG GFA Sbjct: 260 LAMLPFTFIVPVIAGRMSSQRLLVVITTVLLLTGTLGLLYGSSNVILLWIIILGIGGGFA 319 Query: 334 FGLSMMFFGLRTRSAHQAAELSGMAQSIGYLLAAMGPALIGYLHDATHSWSVPFLILACA 393 F L+MMFFGLRT +AHQAAELSGMAQS+GYLLAA+GPALIGYLHDAT+SW++P IL A Sbjct: 320 FSLAMMFFGLRTDNAHQAAELSGMAQSVGYLLAAIGPALIGYLHDATNSWNLPLFILLGA 379 Query: 394 SALLGIIGLGAARNRFV 410 S LL ++G+GAA NR V Sbjct: 380 SVLLFLVGIGAASNRVV 396 >C0Z4P7.1 C0Z4P7_BREBN Conserved hypothetical membrane protein Length = 417 Score = 498 bits (1281), Expect = e-139, Method: Compositional matrix adjust. Identities = 264/401 (65%), Positives = 311/401 (77%), Gaps = 2/401 (0%) Query: 8 LKKQDAISTTGIRKQARIWFMIAGIIFIAANLRAPLTSVGPLVGLIRENVHISNTLAGLI 67 + Q+ S + + A +W +I GIIFIAANLRAPLTSVGPLVGLIR++VHISNTLAG+I Sbjct: 1 MSTQEMKSKPNVARSA-VWVVIVGIIFIAANLRAPLTSVGPLVGLIRDDVHISNTLAGMI 59 Query: 68 TTVPLIAFALLSPFVPKLGRRYGVERIMLISLILLVIGIAVRSLSGAVTLFVGTAVLGFA 127 TT+PL+AFAL SP VPKLGRR+GVE I+LIS+ILL G+ +RS SG TL++GTAVLG A Sbjct: 60 TTLPLLAFALFSPIVPKLGRRFGVEGIILISIILLTFGVILRSFSGIGTLYIGTAVLGLA 119 Query: 128 ITICNVLLPSLIKREFPLQMGTMTGVYSVSMNLSGAIASGISVPLAVGAGLNWQGALGVW 187 I++ NVLLPSLIKREFP Q+G MTGVYS+SMNL GAIASGISVP+AVG G+ W+GALG+W Sbjct: 120 ISVSNVLLPSLIKREFPRQIGLMTGVYSISMNLLGAIASGISVPIAVGLGVGWRGALGIW 179 Query: 188 GILSVISILFWAPQLKXXXXXXXXXXXXXGNDHHVNIWRSPLAWQVTLFMGLQSTIFYVL 247 ILS++S+LFW PQ+K D VNIWRS LAWQVTLFMGLQST FYVL Sbjct: 180 AILSLVSVLFWLPQMKRRNRETATIQNQSA-DSSVNIWRSALAWQVTLFMGLQSTFFYVL 238 Query: 248 VAWLPEILKDQGISSSQSGWFLSIMIIASLPLSFIVPVIAGRMRNQRXXXXXXXXXXXXX 307 + WLPEILK QGISS Q+GWFLS+M +A LP +FIVP+IAGRM +QR Sbjct: 239 ITWLPEILKQQGISSDQAGWFLSVMQLAVLPFTFIVPIIAGRMSSQRLLVTITAILLLMG 298 Query: 308 XXXXXYGSIHLVLLWTIILGIGSGFAFGLSMMFFGLRTRSAHQAAELSGMAQSIGYLLAA 367 YGS + ++ W I+LGIG GFAF LSMMFFGLRT +A QAAELSGMAQS+GYLLAA Sbjct: 299 TLGLLYGSANFIVWWIIMLGIGGGFAFSLSMMFFGLRTENAQQAAELSGMAQSVGYLLAA 358 Query: 368 MGPALIGYLHDATHSWSVPFLILACASALLGIIGLGAARNR 408 +GP L GYLHDAT SW+VP L+L CAS LL + GLGAARNR Sbjct: 359 IGPTLFGYLHDATDSWTVPLLLLVCASILLFVFGLGAARNR 399 >A7GF62.1 A7GF62_CLOBL Transporter, major facilitator family Length = 414 Score = 447 bits (1150), Expect = e-124, Method: Compositional matrix adjust. Identities = 238/410 (58%), Positives = 300/410 (73%), Gaps = 4/410 (0%) Query: 1 MSSSEQLLKKQDAISTTGIRKQARIWFMIAGIIFIAANLRAPLTSVGPLVGLIRENVHIS 60 M S + KKQ+ I +RK A + +I GIIFIAANLRAPLTSVGPLV IR+N+HIS Sbjct: 1 MMSLNKSDKKQNNIDFK-VRKSASL-MLIIGIIFIAANLRAPLTSVGPLVKFIRDNLHIS 58 Query: 61 NTLAGLITTVPLIAFALLSPFVPKLGRRYGVERIMLISLILLVIGIAVRSLSGAVTLFVG 120 NTLAG+ITT+PL AFA+ SP P+LGR++GVE ++L+S+I L +GI +RSLSG LF+G Sbjct: 59 NTLAGMITTLPLFAFAIFSPVAPRLGRKFGVELVLLLSVIFLTVGIILRSLSGVAGLFIG 118 Query: 121 TAVLGFAITICNVLLPSLIKREFPLQMGTMTGVYSVSMNLSGAIASGISVPLAVGAGLNW 180 TA++G AI++ NVL+PSLIKR+FP ++G MTGVYS+SMN GAIASGIS+P+A G G W Sbjct: 119 TAIIGLAISVANVLIPSLIKRDFPEKIGVMTGVYSISMNTFGAIASGISIPIATGLGFKW 178 Query: 181 QGALGVWGILSVISILFWAPQLKXXXXXXXXXXXXXGNDHHVNIWRSPLAWQVTLFMGLQ 240 GALG+WGILS ISI+ W PQ+K + +V++W+S LAWQVTLFMGLQ Sbjct: 179 SGALGIWGILSFISIILWLPQVK--RNDGEASKGHKADSSNVSLWKSSLAWQVTLFMGLQ 236 Query: 241 STIFYVLVAWLPEILKDQGISSSQSGWFLSIMIIASLPLSFIVPVIAGRMRNQRXXXXXX 300 S +FY +VAW+PEIL +G+SS+++GW LS+M +A +P +FIV VIAGR NQR Sbjct: 237 SLVFYSMVAWMPEILIQKGMSSNKAGWMLSLMQLALIPFTFIVSVIAGRRSNQRSLVISG 296 Query: 301 XXXXXXXXXXXXYGSIHLVLLWTIILGIGSGFAFGLSMMFFGLRTRSAHQAAELSGMAQS 360 YGS V LW IILGIG GFAF LSMMFF LRT ++++AA+LSGMAQS Sbjct: 297 CSFILIGILGLLYGSSQFVFLWIIILGIGGGFAFSLSMMFFSLRTENSNEAAQLSGMAQS 356 Query: 361 IGYLLAAMGPALIGYLHDATHSWSVPFLILACASALLGIIGLGAARNRFV 410 +GYLLAA GP L G+LHDAT+SW VP L + LL I GLGA+RNR+V Sbjct: 357 LGYLLAAFGPILFGFLHDATNSWKVPLQTLIGITVLLFIFGLGASRNRYV 406 >B1KVD2.1 B1KVD2_CLOBM Transporter, major facilitator family Length = 414 Score = 447 bits (1149), Expect = e-123, Method: Compositional matrix adjust. Identities = 237/410 (57%), Positives = 300/410 (73%), Gaps = 4/410 (0%) Query: 1 MSSSEQLLKKQDAISTTGIRKQARIWFMIAGIIFIAANLRAPLTSVGPLVGLIRENVHIS 60 M S + KKQ+ + +RK A + +I GIIFIAANLRAPLTSVGPLV IR+N+HIS Sbjct: 1 MMSLNKSDKKQNNMDFK-VRKSASL-MLIIGIIFIAANLRAPLTSVGPLVKFIRDNLHIS 58 Query: 61 NTLAGLITTVPLIAFALLSPFVPKLGRRYGVERIMLISLILLVIGIAVRSLSGAVTLFVG 120 NTLAG+ITT+PL AFAL SP P+LGR++GVE ++L+S+I L +GI +RSLSG LF+G Sbjct: 59 NTLAGMITTLPLFAFALFSPVAPRLGRKFGVELVLLLSVIFLTVGIILRSLSGVAGLFIG 118 Query: 121 TAVLGFAITICNVLLPSLIKREFPLQMGTMTGVYSVSMNLSGAIASGISVPLAVGAGLNW 180 TA++G AI++ NVL+PSLIKR+FP ++G MTGVYS+SMN GAIASGIS+P+A G G W Sbjct: 119 TAIIGLAISVANVLIPSLIKRDFPEKIGVMTGVYSISMNTFGAIASGISIPIATGLGFKW 178 Query: 181 QGALGVWGILSVISILFWAPQLKXXXXXXXXXXXXXGNDHHVNIWRSPLAWQVTLFMGLQ 240 GALG+WGILS ISI+ W PQ+K + +V++W+S LAWQVTLFMGLQ Sbjct: 179 SGALGIWGILSFISIILWLPQVK--RNDGEASKGHKADSSNVSLWKSSLAWQVTLFMGLQ 236 Query: 241 STIFYVLVAWLPEILKDQGISSSQSGWFLSIMIIASLPLSFIVPVIAGRMRNQRXXXXXX 300 S +FY +VAW+PEIL +G+SS+++GW LS+M +A +P +FIV V+AGR NQR Sbjct: 237 SLVFYSMVAWMPEILMQKGMSSNKAGWMLSLMQLALIPFTFIVSVLAGRRSNQRSLVISG 296 Query: 301 XXXXXXXXXXXXYGSIHLVLLWTIILGIGSGFAFGLSMMFFGLRTRSAHQAAELSGMAQS 360 YGS V LW IILGIG GFAF LSMMFF LRT ++++AA+LSGMAQS Sbjct: 297 CLFILIGILGLLYGSSQFVFLWIIILGIGGGFAFSLSMMFFSLRTENSNEAAQLSGMAQS 356 Query: 361 IGYLLAAMGPALIGYLHDATHSWSVPFLILACASALLGIIGLGAARNRFV 410 +GYLLAA GP L G+LHDAT+SW VP L + LL I GLGA+RNR+V Sbjct: 357 LGYLLAAFGPILFGFLHDATNSWKVPLQTLIGITVLLFIFGLGASRNRYV 406 >B1IG39.1 B1IG39_CLOBK Transporter, major facilitator family Length = 414 Score = 444 bits (1143), Expect = e-123, Method: Compositional matrix adjust. Identities = 237/410 (57%), Positives = 300/410 (73%), Gaps = 4/410 (0%) Query: 1 MSSSEQLLKKQDAISTTGIRKQARIWFMIAGIIFIAANLRAPLTSVGPLVGLIRENVHIS 60 M S + KKQ+ + +RK A + +I GIIFIAANLRAPLTSVGPLV IR+N+HIS Sbjct: 1 MMSLNKSDKKQNNMDFK-VRKSASL-MLIIGIIFIAANLRAPLTSVGPLVKFIRDNLHIS 58 Query: 61 NTLAGLITTVPLIAFALLSPFVPKLGRRYGVERIMLISLILLVIGIAVRSLSGAVTLFVG 120 NTLAG+ITT+PL AFA+ SP P+LGR++GVE ++L+S+I L +GI +RSLSG LF+G Sbjct: 59 NTLAGMITTLPLFAFAIFSPIAPRLGRKFGVELVLLLSVIFLTVGIILRSLSGVAGLFIG 118 Query: 121 TAVLGFAITICNVLLPSLIKREFPLQMGTMTGVYSVSMNLSGAIASGISVPLAVGAGLNW 180 TA++G AI++ NVL+PSLIKR+FP ++G MTGVYS+SMN GAIASGISVP+A GL W Sbjct: 119 TAIIGLAISVANVLIPSLIKRDFPEKIGVMTGVYSISMNTFGAIASGISVPIATRLGLKW 178 Query: 181 QGALGVWGILSVISILFWAPQLKXXXXXXXXXXXXXGNDHHVNIWRSPLAWQVTLFMGLQ 240 GALG+WGILS ISI+ W PQ+K + +V++W+S LAWQVTLFMGLQ Sbjct: 179 SGALGIWGILSFISIILWLPQVK--RNDGEVSKGHKADSSNVSLWKSSLAWQVTLFMGLQ 236 Query: 241 STIFYVLVAWLPEILKDQGISSSQSGWFLSIMIIASLPLSFIVPVIAGRMRNQRXXXXXX 300 S +FY +VAW+PEIL +G+SS+++GW LS+M +A +P +FIV V+AGR NQR Sbjct: 237 SLVFYSMVAWMPEILMQKGMSSNKAGWMLSLMQLALIPFTFIVSVLAGRRSNQRSLVISG 296 Query: 301 XXXXXXXXXXXXYGSIHLVLLWTIILGIGSGFAFGLSMMFFGLRTRSAHQAAELSGMAQS 360 YGS V LW IILGIG GFAF LSMMFF LRT ++++AA+LSGMAQS Sbjct: 297 CSFILIGILGLLYGSSQFVFLWIIILGIGGGFAFSLSMMFFSLRTENSNEAAQLSGMAQS 356 Query: 361 IGYLLAAMGPALIGYLHDATHSWSVPFLILACASALLGIIGLGAARNRFV 410 +GYLLAA GP L G+LHDAT+SW VP L + LL I GLGA+RNR+V Sbjct: 357 LGYLLAAFGPILFGFLHDATNSWKVPLQTLIGITVLLFIFGLGASRNRYV 406 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.327 0.141 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,166,524,427 Number of extensions: 84195733 Number of successful extensions: 414372 Number of sequences better than 1.0e-05: 681 Number of HSP's gapped: 413083 Number of HSP's successfully gapped: 712 Length of query: 411 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 273 Effective length of database: 1,760,596,133 Effective search space: 480642744309 Effective search space used: 480642744309 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)