BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0427.0005.p (521 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KH90.1 B3KH90_9BACI Extracellular solute-binding protein family 1 346 4e-93 B1D6I7.1 B1D6I7_9BACL Extracellular solute-binding protein family 1 169 8e-40 B3K759.1 B3K759_9BACI Extracellular solute-binding protein family 1 147 2e-33 Q71Y14.1 Q71Y14_LISMF ABC transporter, substrate-binding protein 132 8e-29 C1KWV1.1 C1KWV1_LISMC Putative sugar-binding lipoprotein 132 8e-29 >B3KH90.1 B3KH90_9BACI Extracellular solute-binding protein family 1 Length = 505 Score = 346 bits (887), Expect = 4e-93, Method: Compositional matrix adjust. Identities = 190/519 (36%), Positives = 288/519 (55%), Gaps = 20/519 (3%) Query: 2 KKSYVILLAVVLLFSTLIGCSQGSNSTNTADKEDNSSAAKPENNGKPAETVKIKYLVPGT 61 +K+ + LA + + S + GCS G D + +A EN G + + ++YL+PG+ Sbjct: 4 RKALALWLAAIFMVSVIAGCSGG------GDAGSENGSASGENGG--GDKLPVRYLLPGS 55 Query: 62 EPKDYKEVFQKVNEKLAADGVGVEVEKIYIPWDAWDQKLNLMLSTGEDFDLFHVMQDRTP 121 P+D + +NEKL ADG+ + E YI WD WDQK NLM+STGE+F+L +M D Sbjct: 56 APQDLDAAVKAINEKLEADGLNLTYEPTYIAWDVWDQKTNLMMSTGEEFELIAIMHDTKG 115 Query: 122 FSSYYNRGALADITDAIEKYGQNLKKHIPDDIFDGAKIDGKYYAAPSYWVEMA-SEGQFD 180 + G + I D +++YG LK +PD I++ +KI+GK P++W + A ++G F Sbjct: 116 PTVLAGSGGIIPIDDLLDQYGTELKASMPDWIWESSKINGKITYVPNFWADTAYNDGMFT 175 Query: 181 IRRDILRENNLKEPTTPAELISAWETVMKNWKGSNKPYLGTRADFDPIFLHTSILHRTYD 240 IR D+L +N LK PT+P EL++A ET+ KNW NK + FLHT TYD Sbjct: 176 IRTDLLEKNGLKPPTSPEELLNAAETLQKNWPQDNKNVYIKMLEEPAYFLHT-----TYD 230 Query: 241 TFPFTVKDKFFYVNQNGEVKSWIETEEFKKDASFMRELYTKGITNPDILVMKQEQVDAQL 300 T+PFTV D Y++Q G VKSW ETEEFKKD FM E Y +G+ D+L E ++ + Sbjct: 231 TYPFTVADNMIYIDQQGNVKSWFETEEFKKDVKFMNEAYKRGLIMSDLLTTPTEVINQEE 290 Query: 301 DSGDWFVRXXXXXXXXXXKKYNPEATVNDIGVVWFNPEKEYLRPLTFKNGNAVPVNSKHP 360 +G + R + P+A ++ +++ ++ R +N N + S HP Sbjct: 291 LAGRYLYRPGDVGLSDAILQTFPDAKLD----IYYLADQPKFRSYAIRNSNGISATSPHP 346 Query: 361 EAAVKFMDWMLASQENYDLVQYGIEGKHYTKDGEKGYKPIKDXXXXXXXGYRGSDSQNGN 420 EA ++F++W+ +SQEN DLVQ G++ H+ KD K K Y G+ Sbjct: 347 EAGIQFLNWVYSSQENMDLVQSGVKDVHW-KDTGKNTKDYLAKNENGSPAYELPYWLLGH 405 Query: 421 VNFMRFDLENSIPDNNKVLFEPNPNAVNSIAANFIFDPTNVRAEYTNIMSEASASITPIY 480 V R+ N+ P + + +AVNSI F FDPTNV ++Y N ++E S+ P+ Sbjct: 406 VEMNRYP-TNTDPARLERRTTVSEDAVNSITIGFTFDPTNVASQYANCIAELKTSVYPVM 464 Query: 481 MGVLEYDKAFPAALEKMRKAGLDKVVAEYQKQFQEYQAS 519 GV++Y+ A+ L M+ AGLD+VV EY++QF E++A Sbjct: 465 HGVVKYEDAYDKMLAAMKSAGLDEVVDEYKRQFDEWRAG 503 >B1D6I7.1 B1D6I7_9BACL Extracellular solute-binding protein family 1 Length = 514 Score = 169 bits (427), Expect = 8e-40, Method: Compositional matrix adjust. Identities = 151/529 (28%), Positives = 257/529 (48%), Gaps = 51/529 (9%) Query: 6 VILLAVVLLFSTL-----IGCSQGSNSTNTADKEDNSSAAKPENNGKPAETVKIKYLVPG 60 V +L++ L+F+ +G S+ N DN+ PE+ V +K ++PG Sbjct: 17 VAILSLSLVFALFSQYINLGSSKSPGPHNPG-TTDNAGKDAPES------FVTLKVIMPG 69 Query: 61 TEPKDYKE-VFQKVNEKLAADGVGVEVEKIYIPWDAWDQKLNLMLSTGEDFDLFHVMQDR 119 + + V ++N++L D + + +E YIPW + QKL L LSTGED+DLF Sbjct: 70 DQSSRMGDFVENELNDRLKKD-LNMNLELTYIPWSNYQQKLELALSTGEDYDLFWY---G 125 Query: 120 TPFSS-YYNRGALADITDAIEKYGQNLKKHIPDDIFDGAKIDGKYYAAPSYWVEMASEGQ 178 PF S YY++G + + + + +YGQ+L KHIP+D F +IDGK +A PS S G+ Sbjct: 126 APFVSVYYSKGYIQPLDELLGQYGQDLIKHIPEDNFKQNRIDGKLWAIPSQ--AFTSAGK 183 Query: 179 FD---IRRDILRENNLKEPTTPAELISAWETVMKNWKGSNKPYLGTRADFDPIFLHTSIL 235 F +R+D+L + + + T A+L ++ M S YL T D L + Sbjct: 184 FSSVMVRQDLLEKVGMTDIKTIADLEQFYQR-MHAIDSSYYGYLET--DRGHELLWRELT 240 Query: 236 HRTYDTFPFTVKDKFFYVNQN-GEVKSWIETEEFKKDASFMRELYTKGITNPDILVMKQE 294 + Y + + F V+++ GE+ S++E++ +KK A KGI + +++ Sbjct: 241 DQNYTSLD---ERMLFMVDEDSGELLSYVESDLYKKVARLRESWVNKGIIDMNLVGNPAA 297 Query: 295 QVDAQLDSGDWFVR----XXXXXXXXXXKKYNPEATVNDIGVVWFNPEKEYLRPLTFKNG 350 ++D Q ++G R + +PEA + + + KE + +T + Sbjct: 298 KID-QENAGKLLFRVGAVSRAMENLQTARNADPEARLRE-----YYLAKEKPKYITTPSN 351 Query: 351 NAVPVNS--KHPEAAVKFMDWMLASQENYDLVQYGIEGKHYTKDGEKGYKPIKDXXXXXX 408 A + S K+PE A+ FM+W+L S+ENY+ + YG+EGK Y + K K + + Sbjct: 352 EAFMITSASKNPERAMMFMNWILQSKENYNFIIYGVEGKDYVLENGK-IKQLTNDQLMYE 410 Query: 409 XGYRGSDSQNGNVNFMRFDLENSIPDNNKVLFEPNPNAVNSIAANFIFDPTNVRAEYTNI 468 +R D N +E+ + ++ + + N+ S + F F+ V+ E + Sbjct: 411 WMWRNKDYFIAPAN-----VEDDVIED---MLANDENSRISKLSGFHFNEGPVKNEIAQV 462 Query: 469 MSEASASITPIYMGVLEYDKAFPAALEKMRKAGLDKVVAEYQKQFQEYQ 517 M+ PI G+ YD+ + AL ++KAG D V AE +KQF E+Q Sbjct: 463 MAVYKEKFEPINFGIASYDQYYDDALAALKKAGFDIVFAELKKQFSEFQ 511 >B3K759.1 B3K759_9BACI Extracellular solute-binding protein family 1 Length = 485 Score = 147 bits (372), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 147/546 (26%), Positives = 245/546 (44%), Gaps = 93/546 (17%) Query: 2 KKSYVILLAVVLLFSTLIGCSQGSNSTNTADKEDNSSAAKPENNGKPAETVKIKYLVPGT 61 KK ++L+ ++ S + GCS K++ ++A ETV I Y PG Sbjct: 4 KKMLILLVLLIASVSVMAGCS---------GKKEGAAAL---------ETVTILY--PGE 43 Query: 62 EPKDYKEVFQ-KVNEKLAADGVGVEVEKIYIPW-DAWDQKLNLMLSTGEDFDLFHVMQDR 119 E + EK+AAD +G++VE IY+PW W+QK ++ML+ E DL+ D Sbjct: 44 RSDRMNEFLNNEFAEKMAAD-LGLKVEVIYVPWAQYWEQK-DIMLAANEPIDLYW---DG 98 Query: 120 TP-FSSYYNRGALADITDAIEKYGQNLKKHIPDDIFDGAKIDGKYYAAPSYWVEMASEGQ 178 P S+ N+ + D I++YGQ++ K +P + GA IDGK + PS + ++ Q Sbjct: 99 LPDLSTIVNKKQAMVLDDLIQEYGQDMLKVLPMEQLQGATIDGKIHGIPSAYAPSSAMYQ 158 Query: 179 F-DIRRDILRENNLKEPTTPAELISAWETVMKNWKGSNKPYLGTRAD--FDPIFLHTSIL 235 +R+DIL + + TP +L + T K N P + AD F P+ + + Sbjct: 159 LVAVRQDILEAVGMTDVKTPDDL-KEFATKAKE----NFPEMKGPADIIFKPLTRYFTDE 213 Query: 236 HRTYDTFPFTVKDKFFYVNQNGEVKSWIETEEFKKDASFMRELYTKGITNPDILVMKQEQ 295 + V+D + + + S+ ETE F++ A F R +Y G+ D L +K + Sbjct: 214 QYNW----IAVEDLVVFGEDSKKAYSYYETEAFQEVAKFNRGMYEAGLYTED-LTIKYNE 268 Query: 296 VDAQLDSGDWF----VRXXXXXXXXXXKKYNPEATVNDIGVVWFNPEKEYLRPLTFKNGN 351 D+++ +G + K P+A V + P R +T G Sbjct: 269 RDSRMQTGLYLWVEGSLGKEMEIVDAIKANAPDAEVKTYLLAEDKP-----RYVTATGGE 323 Query: 352 --AVPVNSKHPEAAVKFMDWMLASQENYDLVQYGIEGKHYTKDGEKGYKPIKDXXXXXXX 409 +PVN+ +PE A+KF++W+ S++NY YG+EGK Y Sbjct: 324 VLGIPVNAPNPEGAMKFVNWIYKSKDNYQFALYGVEGKDY-------------------- 363 Query: 410 GYRGSDSQNGNVNFM---RFDLENSIPDNNKVLFEPN-------------PNAVNSIAAN 453 + +G +N + F E + N LF PN AV S + Sbjct: 364 -----EIVDGRINKLTPSEFFYEWMFRNQNYQLFGPNVAQESIDQYKSWDDQAVRSESLG 418 Query: 454 FIFDPTNVRAEYTNIMSEASASITPIYMGVLEYDKAFPAALEKMRKAGLDKVVAEYQKQF 513 F F+ V+ T++ A + I G ++++ +P A++K++ AG+D+ VAE Q+Q Sbjct: 419 FRFNNEKVKLIETSLKEVAGKDLAAIRSGFVDFETEYPKAIQKLKAAGIDEYVAEIQRQL 478 Query: 514 QEYQAS 519 E+ A Sbjct: 479 DEFLAG 484 >Q71Y14.1 Q71Y14_LISMF ABC transporter, substrate-binding protein Length = 485 Score = 132 bits (333), Expect = 8e-29, Method: Compositional matrix adjust. Identities = 148/539 (27%), Positives = 236/539 (43%), Gaps = 76/539 (14%) Query: 1 MKKSYVILLAVVLLFSTLIGCSQGSNSTNTADKEDNSSAAKPENNGKPAETVKIKYLVPG 60 MKK + +++A + L L C GS+ A+ E +K+ + G Sbjct: 1 MKKKWGVIIASLCLLLGLSAC--GSDEKADAN-----------------EVPTLKWYMIG 41 Query: 61 TEPKDYKEVFQKVNEKLAADGVGVEVEKIYIPWDAWDQKLNLMLSTGEDFDLFHVMQDRT 120 T KD +EV ++VN K + +GV+VE I W + +++ ++++GE+FD+ + Sbjct: 42 TPQKDTEEVMKEVN-KYTEEKIGVKVEMTQIDWGDYGKRMQTVINSGENFDIAYSAAGE- 99 Query: 121 PFSSYYNRGALADITDAIEKYGQNLKKHIPDDIFDGAKIDGKYYAAPSYWVEMASEGQFD 180 F SY +GA + ++K G+N+K + D +++GA IDG Y PS E+ + + Sbjct: 100 -FVSYSQKGAFLPLNKYLDKEGKNMKAELNDVLWEGATIDGNIYGVPSN-KEVGEQQVYV 157 Query: 181 IRRDILRENNLKEPTTPAELISAWETVMKNWKGSNKPY---LGTRADFDPIFLHTSILHR 237 ++++ + K T + S E ++K K N+P +G DF P F + ++ Sbjct: 158 FNKNLV--DKYKMDVTKVKNFSDLEPMLKTIK-ENEPSITPIGGNKDFKPAFPYDYLIDN 214 Query: 238 TYDTFPFTVKDKFFYVNQNGEVKSWIETEEFKKDASFMRELYTKGITNPDILVMKQEQVD 297 PF V G++ ++ E E + Y KG DI Sbjct: 215 AV-PLPFAVNQ----YEDTGKIVNFYEQPETLDILKSLHSFYQKGYVTKDIATSTDPW-- 267 Query: 298 AQLDSGDWFVRXXXXXXXXXXKKYNPEA-------TVNDIGVVWFNPEKEYLRPLTFKNG 350 D +WFVR +KY P A T VV P E P+ N Sbjct: 268 -SYDKENWFVR---------IEKYQPYAENIWDKNTGGKYKVV-TQPIAE--TPIIKNNS 314 Query: 351 -----NAVPVNSKHPEAAVKFMDWMLASQENYDLVQYGIEGKHYTK--DGEKGYKPIKDX 403 AV V S+HPE AVKF+D + + +LV GIEG HY + DG+ P + Sbjct: 315 VTGAIQAVSVTSQHPEEAVKFLDLLNTDEYLRNLVDKGIEGTHYKELDDGKIQDLPAR-- 372 Query: 404 XXXXXXGYRGSDSQNGNVNFMRFDLENSIPDNNKVLFEPNPNAVNSIAANFIFDPTNVRA 463 Y GN +F+ E+ D E N +V S F FD + VR Sbjct: 373 ----VERYSMPTYALGN-HFILKLYEDEPADKWDQFKEFNRKSVASPGLGFYFDSSKVRT 427 Query: 464 E---YTNIMSEASASITPIYMGVLEYDKAFPAALEKMRKAGLDKVVAEYQKQFQEYQAS 519 E TN+ +E + ++ + +EY + F A K+ AG+ KV+ E QKQ+ E++AS Sbjct: 428 EIASITNVCNEFAPALLTGTVDPVEYTEKFKA---KLNDAGMKKVMKEMQKQYDEFRAS 483 >C1KWV1.1 C1KWV1_LISMC Putative sugar-binding lipoprotein Length = 485 Score = 132 bits (333), Expect = 8e-29, Method: Compositional matrix adjust. Identities = 148/539 (27%), Positives = 236/539 (43%), Gaps = 76/539 (14%) Query: 1 MKKSYVILLAVVLLFSTLIGCSQGSNSTNTADKEDNSSAAKPENNGKPAETVKIKYLVPG 60 MKK + +++A + L L C GS+ A+ E +K+ + G Sbjct: 1 MKKKWGVIIASLCLLLGLSAC--GSDEKADAN-----------------EVPTLKWYMIG 41 Query: 61 TEPKDYKEVFQKVNEKLAADGVGVEVEKIYIPWDAWDQKLNLMLSTGEDFDLFHVMQDRT 120 T KD +EV ++VN K + +GV+VE I W + +++ ++++GE+FD+ + Sbjct: 42 TPQKDTEEVMKEVN-KYTEEKIGVKVEMTQIDWGDYGKRMQTVINSGENFDIAYSAAGE- 99 Query: 121 PFSSYYNRGALADITDAIEKYGQNLKKHIPDDIFDGAKIDGKYYAAPSYWVEMASEGQFD 180 F SY +GA + ++K G+N+K + D +++GA IDG Y PS E+ + + Sbjct: 100 -FVSYSQKGAFLPLNKYLDKEGKNMKAELNDVLWEGATIDGNIYGVPSN-KEVGEQQVYV 157 Query: 181 IRRDILRENNLKEPTTPAELISAWETVMKNWKGSNKPY---LGTRADFDPIFLHTSILHR 237 ++++ + K T + S E ++K K N+P +G DF P F + ++ Sbjct: 158 FNKNLV--DKYKMDVTKVKNFSDLEPMLKTIK-ENEPSITPIGGNKDFKPAFPYDYLIDN 214 Query: 238 TYDTFPFTVKDKFFYVNQNGEVKSWIETEEFKKDASFMRELYTKGITNPDILVMKQEQVD 297 PF V G++ ++ E E + Y KG DI Sbjct: 215 AV-PLPFAVNQ----YEDTGKIVNFYEQPETLDILKSLHSFYQKGYVTKDIATSTDPW-- 267 Query: 298 AQLDSGDWFVRXXXXXXXXXXKKYNPEA-------TVNDIGVVWFNPEKEYLRPLTFKNG 350 D +WFVR +KY P A T VV P E P+ N Sbjct: 268 -SYDKENWFVR---------IEKYQPYAENIWDKNTGGKYKVV-TQPIAE--TPIIKNNS 314 Query: 351 -----NAVPVNSKHPEAAVKFMDWMLASQENYDLVQYGIEGKHYTK--DGEKGYKPIKDX 403 AV V S+HPE AVKF+D + + +LV GIEG HY + DG+ P + Sbjct: 315 VTGAIQAVSVTSQHPEEAVKFLDLLNTDEYLRNLVDKGIEGTHYKELDDGKIQDLPAR-- 372 Query: 404 XXXXXXGYRGSDSQNGNVNFMRFDLENSIPDNNKVLFEPNPNAVNSIAANFIFDPTNVRA 463 Y GN +F+ E+ D E N +V S F FD + VR Sbjct: 373 ----VERYSMPTYALGN-HFILKLYEDEPADKWDQFKEFNRKSVASPGLGFYFDSSKVRT 427 Query: 464 E---YTNIMSEASASITPIYMGVLEYDKAFPAALEKMRKAGLDKVVAEYQKQFQEYQAS 519 E TN+ +E + ++ + +EY + F A K+ AG+ KV+ E QKQ+ E++AS Sbjct: 428 EIASITNVCNEFAPALLTGTVDPVEYTEKFKA---KLNDAGMKKVMKEMQKQYDEFRAS 483 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.133 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,221,029,336 Number of extensions: 140786245 Number of successful extensions: 272485 Number of sequences better than 1.0e-05: 223 Number of HSP's gapped: 272218 Number of HSP's successfully gapped: 285 Length of query: 521 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 381 Effective length of database: 1,741,754,103 Effective search space: 663608313243 Effective search space used: 663608313243 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 134 (56.2 bits)