BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0427.0004.p (290 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KH93.1 B3KH93_9BACI Binding-protein-dependent transport system... 309 2e-82 A4XN07.1 A4XN07_CALS8 Binding-protein-dependent transport system... 239 2e-61 B1D9U6.1 B1D9U6_9BACL Binding-protein-dependent transport system... 233 1e-59 B1D7R3.1 B1D7R3_9BACL Binding-protein-dependent transport system... 229 3e-58 B3K760.1 B3K760_9BACI Binding-protein-dependent transport system... 226 2e-57 >B3KH93.1 B3KH93_9BACI Binding-protein-dependent transport systems inner membrane component Length = 292 Score = 309 bits (792), Expect = 2e-82, Method: Compositional matrix adjust. Identities = 152/258 (58%), Positives = 197/258 (76%), Gaps = 5/258 (1%) Query: 35 TDEDAILKNGYSLFPEKFSLYAYKLIFTGGSQVMQSYGISIFVTIVGTALAILITSMAGY 94 TDE +I + GY LFP + SL AYKL+ G + + +SY ISI VT+VGT LA+ ITSMAGY Sbjct: 36 TDEASIKQFGYQLFPSELSLDAYKLLLFGSTPLYRSYMISIIVTVVGTILAVTITSMAGY 95 Query: 95 TLANKNVKYRNLLALYFFITMIFSAGIVPWYLMNRSLGLTNNILALIIXXXXXXXXXXXX 154 TLA K+++YRN L L+FFITM+F+ G+VPWYL+N +LG+ N I ALII Sbjct: 96 TLAAKHIRYRNGLGLFFFITMVFNTGLVPWYLINLNLGMNNTIWALIIPSLVFNPFNLFL 155 Query: 155 VRNFMNGVPDSLRESATIDGASDITIAFRIYLPLCKPVLATIALFYGLDYWNNWWNAIML 214 VRN+M+ +P SL E+A IDGASD IAFRIY PLC PVLATI LFYG+ YWNNW+NAIML Sbjct: 156 VRNYMSQIPPSLTEAAKIDGASDFYIAFRIYFPLCMPVLATITLFYGIAYWNNWFNAIML 215 Query: 215 IDSKELYPLQFMLLQMQSEISMLNEMT---MLAGSSDITLPSESVKMATAVVTIGPIIFL 271 +D+++LYPLQ++L ++ SEISM+ +M + GS TLP ESVKMATA++TIGP++ Sbjct: 216 VDNEKLYPLQYLLFKLNSEISMIQQMQSSGAILGSQ--TLPQESVKMATAILTIGPVVLF 273 Query: 272 YPYLQKYFVKGLVIGSVK 289 YP+LQ+YF+KGL+IGS+K Sbjct: 274 YPFLQRYFIKGLLIGSLK 291 >A4XN07.1 A4XN07_CALS8 Binding-protein-dependent transport systems inner membrane component Length = 294 Score = 239 bits (610), Expect = 2e-61, Method: Compositional matrix adjust. Identities = 125/259 (48%), Positives = 178/259 (68%), Gaps = 10/259 (3%) Query: 37 EDAILKNGYSLFPEKFSLYAYKLIFTGGSQVMQSYGISIFVTIVGTALAILITSMAGYTL 96 E + NG++L P++ S AYK++F G + V+ +Y ++IFVT+VGT L++++TS+ Y L Sbjct: 41 EADLAANGFTLIPKRISFDAYKIVFKG-NVVLNAYKVTIFVTVVGTVLSMIVTSLMAYPL 99 Query: 97 ANKNVKYRNLLALYFFITMIFSAGIVPWY-LMNRSLGLTNNILALIIXXXXXXXXXXXXV 155 + KN+KYRN +A+Y +ITM+FS G+VP Y L+ R L L N I II + Sbjct: 100 SVKNLKYRNYIAMYVYITMLFSGGLVPSYILITRYLHLDNTIWVYIIPSLINPWNLFV-L 158 Query: 156 RNFMNGVPDSLRESATIDGASDITIAFRIYLPLCKPVLATIALFYGLDYWNNWWNAIMLI 215 RNF +PDSL ESA IDGAS++ I ++I LPL KP +AT++LFY L YWN W+ ++ I Sbjct: 159 RNFFGTIPDSLAESARIDGASEVYILWKIILPLSKPAIATLSLFYALGYWNEWFKCVLYI 218 Query: 216 DSKELYPLQFMLLQMQSEISML----NEMTMLAGSSDITLPSESVKMATAVVTIGPIIFL 271 ++LY LQ++++ + +I ML EM M + LP ++V+MAT VVTIGPIIFL Sbjct: 219 TDQKLYTLQYLIMAIMRDIQMLTQYAQEMNM---PVNYILPQQTVRMATTVVTIGPIIFL 275 Query: 272 YPYLQKYFVKGLVIGSVKG 290 YP+LQKYF+KGLVIG+VKG Sbjct: 276 YPFLQKYFIKGLVIGAVKG 294 >B1D9U6.1 B1D9U6_9BACL Binding-protein-dependent transport systems inner membrane component Length = 295 Score = 233 bits (594), Expect = 1e-59, Method: Compositional matrix adjust. Identities = 117/259 (45%), Positives = 173/259 (66%), Gaps = 6/259 (2%) Query: 35 TDEDAILKNGYSLFPEKFSLYAYKLIFTGGSQVMQSYGISIFVTIVGTALAILITSMAGY 94 T E I+ G+SL PE FSL AYK F+ Q+++ Y ++IF+T+ GTA+ + +TSMAG+ Sbjct: 40 TPEIQIISKGFSLIPETFSLDAYKAAFSNPKQILEGYKVTIFITVAGTAIGLFLTSMAGF 99 Query: 95 TLANKNVKYRNLLALYFFITMIFSAGIVPWY-LMNRSLGLTNNILALIIXXXXXXXXXXX 153 L+ K+ KYR+ L+ + + T +FS G++PWY L+ + L L + +ALI+ Sbjct: 100 VLSRKDFKYRDQLSFFIYFTTLFSGGLIPWYILITKHLDLKDTYMALIV-PSLLSAWNII 158 Query: 154 XVRNFMNGVPDSLRESATIDGASDITIAFRIYLPLCKPVLATIALFYGLDYWNNWWNAIM 213 ++NFM +PDS+ ESA IDGA + TI R+ LPL P LATI LF L YWN+W+ A + Sbjct: 159 LMKNFMKSIPDSIVESAKIDGAGEFTIYLRLILPLSGPGLATIGLFMALGYWNDWFMANL 218 Query: 214 LIDSKELYPLQFMLLQMQSEISMLNEMTMLAGSSDITL--PSESVKMATAVVTIGPIIFL 271 I S+ YPLQF+L ++ + ++L T +AG+ T P+E++KMA AV+ GPI+FL Sbjct: 219 FITSESHYPLQFLLYKILASAAVLK--TNVAGNLSPTFQPPAETLKMAAAVIATGPIVFL 276 Query: 272 YPYLQKYFVKGLVIGSVKG 290 YP++Q++FVKGL IG+VKG Sbjct: 277 YPFVQRFFVKGLTIGAVKG 295 >B1D7R3.1 B1D7R3_9BACL Binding-protein-dependent transport systems inner membrane component Length = 293 Score = 229 bits (583), Expect = 3e-58, Method: Compositional matrix adjust. Identities = 115/257 (44%), Positives = 176/257 (68%), Gaps = 3/257 (1%) Query: 35 TDEDAILKNGYSLFPEKFSLYAYKLIFTGGSQVMQSYGISIFVTIVGTALAILITSMAGY 94 T E +IL++GYS+ P+ FS+ AYKLIF + + +SYG++IFVT+VGT LA+L+T Y Sbjct: 39 TTESSILRDGYSILPKDFSMEAYKLIFRDDT-IYRSYGVTIFVTLVGTLLAMLVTCALAY 97 Query: 95 TLANKNVKYRNLLALYFFITMIFSAGIVPWYLM-NRSLGLTNNILALIIXXXXXXXXXXX 153 L+ + +KY++ + Y + TM+F G+V YL+ ++ LG+ ++I LII Sbjct: 98 PLSIRTLKYKSQVNFYIYFTMLFHGGLVANYLLISKYLGMKDSIWVLIIPALVSPWNMFL 157 Query: 154 XVRNFMNGVPDSLRESATIDGASDITIAFRIYLPLCKPVLATIALFYGLDYWNNWWNAIM 213 +RNF V +SL ESA IDGA+DI I FRI LP+ P LAT+ LFY + +WN W+ A++ Sbjct: 158 -LRNFFKSVDESLSESAKIDGANDIYIFFRIILPISMPALATVGLFYSMGFWNKWFEAML 216 Query: 214 LIDSKELYPLQFMLLQMQSEISMLNEMTMLAGSSDITLPSESVKMATAVVTIGPIIFLYP 273 I ++L+PLQ++++++ S N+++ + +P+ + ++AT VVTIGPIIFLYP Sbjct: 217 YISDEKLFPLQYLVMRIMSNAEFANQLSTQISLPNYVVPTLTTRLATTVVTIGPIIFLYP 276 Query: 274 YLQKYFVKGLVIGSVKG 290 +LQKYFVKGL++G+VKG Sbjct: 277 FLQKYFVKGLMVGAVKG 293 >B3K760.1 B3K760_9BACI Binding-protein-dependent transport systems inner membrane component Length = 298 Score = 226 bits (575), Expect = 2e-57, Method: Compositional matrix adjust. Identities = 119/258 (46%), Positives = 178/258 (68%), Gaps = 4/258 (1%) Query: 35 TDEDAILKNGYSLFPEKFSLYAYKLIFT-GGSQVMQSYGISIFVTIVGTALAILITSMAG 93 + E A+ +NGYS+ P SL YK IF G ++++SYG+++FVT VGT A+LITSM Sbjct: 41 SSEQAVARNGYSIIPLSPSLDTYKYIFVNSGMKLLKSYGVTLFVTTVGTFGALLITSMIA 100 Query: 94 YTLANKNVKYRNLLALYFFITMIFSAGIVPWYLMNRSL-GLTNNILALIIXXXXXXXXXX 152 ++L+ K +KYRN+LA T+IFSAG++PWY+++ + GL N+ILALI+ Sbjct: 101 FSLSIKKLKYRNVLAFISNFTIIFSAGLIPWYMVSVNYYGLKNSILALILPSIFSVWNMF 160 Query: 153 XXVRNFMNGVPDSLRESATIDGASDITIAFRIYLPLCKPVLATIALFYGLDYWNNWWNAI 212 +R + + SL E+A +DGA+ TI +I LPL K L T+ L Y L YWN+WW+A+ Sbjct: 161 L-MRTYFASISPSLYEAAEMDGANYFTIYVKIALPLSKTALLTVGLMYALQYWNDWWHAL 219 Query: 213 MLIDSKELYPLQFMLLQMQSEISMLNEMTMLAGSS-DITLPSESVKMATAVVTIGPIIFL 271 + I+ ++L+PLQ+ L + S ++ ++ + +G+S +ITLP+E+VKMA V+TIGPIIFL Sbjct: 220 IFINERDLFPLQYFLYNILSNVNAISSGRIPSGASGNITLPAETVKMAITVITIGPIIFL 279 Query: 272 YPYLQKYFVKGLVIGSVK 289 YPY+QKYFV G++ G+VK Sbjct: 280 YPYIQKYFVHGIMAGAVK 297 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.325 0.139 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,328,688,659 Number of extensions: 46599037 Number of successful extensions: 186524 Number of sequences better than 1.0e-05: 4820 Number of HSP's gapped: 180253 Number of HSP's successfully gapped: 4821 Length of query: 290 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 156 Effective length of database: 1,798,280,193 Effective search space: 280531710108 Effective search space used: 280531710108 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 130 (54.7 bits)