BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0427.0003.p (325 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KH92.1 B3KH92_9BACI Binding-protein-dependent transport system... 390 e-107 B1D7R4.1 B1D7R4_9BACL Binding-protein-dependent transport system... 315 3e-84 B3K3M4.1 B3K3M4_9BACI Binding-protein-dependent transport system... 305 6e-81 A4XN08.1 A4XN08_CALS8 Binding-protein-dependent transport system... 290 1e-76 B3KBX7.1 B3KBX7_9BACI Binding-protein-dependent transport system... 285 5e-75 >B3KH92.1 B3KH92_9BACI Binding-protein-dependent transport systems inner membrane component Length = 308 Score = 390 bits (1002), Expect = e-107, Method: Compositional matrix adjust. Identities = 184/309 (59%), Positives = 238/309 (77%), Gaps = 2/309 (0%) Query: 17 KKRRRGFGMLSDIRRNGTSYLLVLPAMAYTFIFGYMTYPYMLIAFQRFNYSKGIFHSEWI 76 KK R FG L +IR NG++YLLV+PA YT +FGY T PYMLIAFQ+FN+ G+F+S W+ Sbjct: 2 KKSR--FGFLREIRSNGSAYLLVVPAAIYTLVFGYFTLPYMLIAFQKFNFKTGLFNSAWV 59 Query: 77 GLKNFEFFFRSNKAFTVTFNTIYLNLLFIIFGTLTALAISLILNELRKKLFVKISQSMML 136 G NF+FFF S +A+ VTFNT+ LN LFII GTL A+A++++ NELR + F +I+QS +L Sbjct: 60 GFDNFKFFFSSPRAWEVTFNTLKLNFLFIIVGTLAAMALAILFNELRSRWFSRITQSTIL 119 Query: 137 FPNFISWIVISYVLYAFFSMDMGIVNKLLNQLGIAPVNWYTEQQAWPAILTIMHVWKGAG 196 FP+F+SW+++SY++Y+ S D GI+N++L I PVNWY Q W ILT VWK G Sbjct: 120 FPHFLSWVIVSYIIYSLLSTDYGILNRILAFFHIDPVNWYASPQYWTWILTTAAVWKDLG 179 Query: 197 MSAVIYLATITGIDETLYEAAEIDGASRWQMCMRITVPLMMPTVVILMLLSVGKIMYGDF 256 M+ VIYLA ITGID++ YEA IDGA+RWQ+ IT+PLM+PT+ IL LL++GKIMYG F Sbjct: 180 MNLVIYLAAITGIDDSYYEAGRIDGATRWQLIRHITIPLMLPTISILTLLALGKIMYGSF 239 Query: 257 GMIYALIGDNGTLYGTTDIIDTYVFRSLRQIGNPSEAMAVGLFQSVVGFILVFGTNAITR 316 MIYA++ DNG LY T D+IDTYVFRSLR IGNP++AMAVGL+QSVVGFILV+G+N I + Sbjct: 240 DMIYAIVKDNGLLYPTVDVIDTYVFRSLRTIGNPAQAMAVGLYQSVVGFILVWGSNKIVK 299 Query: 317 KFFKDGALY 325 K D AL+ Sbjct: 300 KVNPDHALF 308 >B1D7R4.1 B1D7R4_9BACL Binding-protein-dependent transport systems inner membrane component Length = 325 Score = 315 bits (808), Expect = 3e-84, Method: Compositional matrix adjust. Identities = 152/309 (49%), Positives = 218/309 (70%) Query: 17 KKRRRGFGMLSDIRRNGTSYLLVLPAMAYTFIFGYMTYPYMLIAFQRFNYSKGIFHSEWI 76 + R + L ++RRNG YLL+LP + F+F Y+ +++AFQ FN KGI SE++ Sbjct: 17 QARVKPKSFLMEMRRNGLLYLLMLPGILLVFVFAYLPMGGIVVAFQNFNAIKGITGSEFV 76 Query: 77 GLKNFEFFFRSNKAFTVTFNTIYLNLLFIIFGTLTALAISLILNELRKKLFVKISQSMML 136 GLKNFEFF +S + V FNT++LN LFI+FG L +++I+++ +EL K F +I Q+++ Sbjct: 77 GLKNFEFFVQSKEVLHVIFNTLFLNALFILFGALASVSIAVMFSELGGKWFKRIVQTIVT 136 Query: 137 FPNFISWIVISYVLYAFFSMDMGIVNKLLNQLGIAPVNWYTEQQAWPAILTIMHVWKGAG 196 PNF+SW VI+ + A S D G++NK L I P+ ++ E AWP IL ++ +W+GAG Sbjct: 137 LPNFLSWTVIAMFVTALLSSDTGMLNKWLAHFHIGPIAFFNEPAAWPFILVLIKIWQGAG 196 Query: 197 MSAVIYLATITGIDETLYEAAEIDGASRWQMCMRITVPLMMPTVVILMLLSVGKIMYGDF 256 A++YLATITGI+ +YE+A IDGASRW+ IT+PL+ PT++++MLL++G I YGDF Sbjct: 197 FGAIVYLATITGINPEIYESASIDGASRWKKIWYITLPLLKPTIILMMLLAIGGIFYGDF 256 Query: 257 GMIYALIGDNGTLYGTTDIIDTYVFRSLRQIGNPSEAMAVGLFQSVVGFILVFGTNAITR 316 GMIYAL+G N LY TTD+IDTYV+R+L +G+ + AVG+FQSV+GFILV N + R Sbjct: 257 GMIYALVGRNVLLYPTTDVIDTYVYRALMDLGDMGMSAAVGVFQSVIGFILVVTVNQLAR 316 Query: 317 KFFKDGALY 325 KF D AL+ Sbjct: 317 KFSPDSALF 325 >B3K3M4.1 B3K3M4_9BACI Binding-protein-dependent transport systems inner membrane component Length = 311 Score = 305 bits (780), Expect = 6e-81, Method: Compositional matrix adjust. Identities = 150/311 (48%), Positives = 209/311 (67%) Query: 15 LEKKRRRGFGMLSDIRRNGTSYLLVLPAMAYTFIFGYMTYPYMLIAFQRFNYSKGIFHSE 74 +E++RR G + ++ RN YLL LP +A+ +F YM L+AFQ + S GI+ SE Sbjct: 1 MERRRRFGGSFVRELSRNRYLYLLALPGIAFLAVFAYMPMAGHLLAFQNYRLSDGIWGSE 60 Query: 75 WIGLKNFEFFFRSNKAFTVTFNTIYLNLLFIIFGTLTALAISLILNELRKKLFVKISQSM 134 W+G KNFEFFF VT NT++LN LF++ G +A+ +++ LNE+R +LF +I+QS Sbjct: 61 WVGFKNFEFFFNGKDWLRVTLNTVFLNALFLVTGLGSAVVLAIFLNEIRNRLFKRITQSF 120 Query: 135 MLFPNFISWIVISYVLYAFFSMDMGIVNKLLNQLGIAPVNWYTEQQAWPAILTIMHVWKG 194 + P FISW+V+S + FS G++N+ L LG V+WY WP ILT++ WK Sbjct: 121 IFLPYFISWLVVSMMTLTLFSTSEGLINRALAALGYEGVDWYLTPGVWPYILTVISAWKI 180 Query: 195 AGMSAVIYLATITGIDETLYEAAEIDGASRWQMCMRITVPLMMPTVVILMLLSVGKIMYG 254 AG +++I+LA ITGI YE+A I+GASR Q + IT+PLM PTV+IL LL +G+I YG Sbjct: 181 AGYNSIIFLAVITGISSEYYESARIEGASRMQQMLYITLPLMRPTVMILALLGIGRIFYG 240 Query: 255 DFGMIYALIGDNGTLYGTTDIIDTYVFRSLRQIGNPSEAMAVGLFQSVVGFILVFGTNAI 314 DFGMIYA+IGDNG L+ TTD+IDTY FR+LRQ+GN S + AV ++QS +G + + NAI Sbjct: 241 DFGMIYAIIGDNGILFPTTDVIDTYAFRALRQLGNFSMSAAVVVYQSSMGLVTILIFNAI 300 Query: 315 TRKFFKDGALY 325 RK D L+ Sbjct: 301 ARKVDADSRLF 311 >A4XN08.1 A4XN08_CALS8 Binding-protein-dependent transport systems inner membrane component Length = 307 Score = 290 bits (743), Expect = 1e-76, Method: Compositional matrix adjust. Identities = 142/307 (46%), Positives = 215/307 (70%), Gaps = 1/307 (0%) Query: 19 RRRGFGMLSDIRRNGTSYLLVLPAMAYTFIFGYMTYPYMLIAFQRFNYSKGIFHSEWIGL 78 R++G ++ ++ +N YL+ LP + + FIF Y+TY ++IAFQ F+YSKGIF S+++GL Sbjct: 2 RKKG-SVIYELTKNKALYLMTLPGIIFFFIFDYITYIGIIIAFQDFDYSKGIFGSKFVGL 60 Query: 79 KNFEFFFRSNKAFTVTFNTIYLNLLFIIFGTLTALAISLILNELRKKLFVKISQSMMLFP 138 +N F S + FNT+YLNLLFI G + ++ I+++LNE+ ++ K+SQS+ + P Sbjct: 61 RNIRFVIESGDLPRIVFNTLYLNLLFIFTGLIFSIGIAVLLNEITNPIYRKVSQSVSILP 120 Query: 139 NFISWIVISYVLYAFFSMDMGIVNKLLNQLGIAPVNWYTEQQAWPAILTIMHVWKGAGMS 198 +F+SW V++ L S + G++NK+L +G+ P+N+Y + + WP +LTI+ +WKGAG Sbjct: 121 HFLSWTVVAMFLLTLCSPESGLINKVLKSMGLEPINFYGDPRPWPLLLTILRIWKGAGWG 180 Query: 199 AVIYLATITGIDETLYEAAEIDGASRWQMCMRITVPLMMPTVVILMLLSVGKIMYGDFGM 258 ++IYLATITGID L+EAA IDGASR Q IT+P + ++L L+ +G I GDFGM Sbjct: 181 SIIYLATITGIDPELFEAARIDGASRLQCIRYITLPELKQPAILLTLMGLGNIFKGDFGM 240 Query: 259 IYALIGDNGTLYGTTDIIDTYVFRSLRQIGNPSEAMAVGLFQSVVGFILVFGTNAITRKF 318 IYAL+GDN L+ TTD+IDTYVFR+LRQ+ + + AVGL QS+VGFILV N + ++ Sbjct: 241 IYALVGDNSLLFPTTDVIDTYVFRALRQLNDVGMSAAVGLMQSIVGFILVVIVNTLVKRI 300 Query: 319 FKDGALY 325 ++ A++ Sbjct: 301 DEEAAIF 307 >B3KBX7.1 B3KBX7_9BACI Binding-protein-dependent transport systems inner membrane component Length = 307 Score = 285 bits (728), Expect = 5e-75, Method: Compositional matrix adjust. Identities = 139/308 (45%), Positives = 208/308 (67%), Gaps = 4/308 (1%) Query: 19 RRRGFGMLSDIRRNGTSYLLVLPAMAYTFIFGYMTYPYMLIAFQRFNYSKGIFHSEWIGL 78 RR G L R+ YLL+LP +AY I Y M IAF+ N+S GIF S+WIG Sbjct: 3 RRSG---LKRFRKYTPLYLLMLPGIAYLIINNYAPMFGMFIAFKNINFSAGIFESDWIGF 59 Query: 79 KNFEFFFRSNKAFTVTFNTIYLNLLFIIFGTLTALAISLILNELRKKLFVKISQSMMLFP 138 KNFE+ F++ AF +T NTI N FI+ GT+ ++A++++LNE+R K+ + QS+++ P Sbjct: 60 KNFEYLFKTTDAFIITRNTILYNAAFIVIGTVLSIAVAILLNEIRVKVMSRFYQSVIVLP 119 Query: 139 NFISWIVISYVLYAFFSMDMGIVNK-LLNQLGIAPVNWYTEQQAWPAILTIMHVWKGAGM 197 + IS++V+ Y++YA S++ G +NK +L GI ++WYTE + WP ILT++ +WK G Sbjct: 120 HLISFVVVGYLVYAALSLETGFMNKTILPLFGIESISWYTEPKYWPVILTVVQLWKSVGF 179 Query: 198 SAVIYLATITGIDETLYEAAEIDGASRWQMCMRITVPLMMPTVVILMLLSVGKIMYGDFG 257 S +I+LA+I ID YEAA +DGAS+W+ IT+PL+ P +V+L LLS+G++ Y DFG Sbjct: 180 SCIIFLASIISIDPEYYEAARLDGASKWRQIQSITIPLITPVIVMLTLLSIGRMFYSDFG 239 Query: 258 MIYALIGDNGTLYGTTDIIDTYVFRSLRQIGNPSEAMAVGLFQSVVGFILVFGTNAITRK 317 + Y + ++G L TT++IDTYV+RSL +G+ A A G++QS+VGF+LV N I RK Sbjct: 240 LFYQVPMNSGMLNDTTNVIDTYVYRSLMIMGDIGMASAAGVYQSIVGFVLVLSANYIVRK 299 Query: 318 FFKDGALY 325 F ++ AL+ Sbjct: 300 FNRENALF 307 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.329 0.143 0.436 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,929,665,620 Number of extensions: 80039626 Number of successful extensions: 280328 Number of sequences better than 1.0e-05: 5522 Number of HSP's gapped: 276655 Number of HSP's successfully gapped: 5522 Length of query: 325 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 189 Effective length of database: 1,779,438,163 Effective search space: 336313812807 Effective search space used: 336313812807 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 131 (55.1 bits)