BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0422.0018.p (384 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KL02.1 B3KL02_9BACI 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosp... 595 e-168 B1DDU0.1 B1DDU0_9BACL 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosp... 582 e-164 A3IBE7.1 A3IBE7_9BACI 4-hydroxy-3-methylbut-2-en-1-yl diphosphat... 508 e-142 B1HSS4.1 ISPG_LYSSC 4-hydroxy-3-methylbut-2-en-1-yl diphosphate ... 507 e-142 A9VH61.1 ISPG_BACWK 4-hydroxy-3-methylbut-2-en-1-yl diphosphate ... 506 e-141 >B3KL02.1 B3KL02_9BACI 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase EC=1.17.4.3 Length = 369 Score = 595 bits (1535), Expect = e-168, Method: Compositional matrix adjust. Identities = 296/365 (81%), Positives = 315/365 (86%) Query: 6 MFLRHQTRPXXXXXXXXXXXXEVAIQSMCTTKTADVEATVAEILRLEEAGCQLVRVTVNN 65 MFLR TRP EV IQSMCTTKTADVEATVAEILRLEEAGCQ+VRVTVNN Sbjct: 1 MFLRQDTRPVKVGNLTIGGKNEVIIQSMCTTKTADVEATVAEILRLEEAGCQIVRVTVNN 60 Query: 66 XXXXXXXXXXXXRIHIPLVADIHFNYKLALLAIENGIDKVRINPGNIGRRDXXXXXXXXX 125 RIHIPLVADIHFNYKLAL+AIENGIDKVRINPGNIG+R+ Sbjct: 61 EEAAAAIKEIKKRIHIPLVADIHFNYKLALMAIENGIDKVRINPGNIGKREKVEAVVKAC 120 Query: 126 XXXGIPIRIGVNAGSLESHLLEKYGYPTPEAMVESALYHIGILEELDFHDIIVSLKASDV 185 GIPIRIGVNAGSLE+HLLEKYGYPTPEAMVESALYHIGILEELDFHDIIVSLKASDV Sbjct: 121 KEKGIPIRIGVNAGSLENHLLEKYGYPTPEAMVESALYHIGILEELDFHDIIVSLKASDV 180 Query: 186 PMAIEAYRKAAEVIPYPLHLGITESGTLFAGTVKSSAGIGALLSMGIGSTVRISLSADPV 245 PMAI AY KAAEVIPYPLHLGITE+GTLF+GTVKS+AG+GALLS+GIGST+RISLSADPV Sbjct: 181 PMAIAAYTKAAEVIPYPLHLGITEAGTLFSGTVKSAAGLGALLSLGIGSTMRISLSADPV 240 Query: 246 EEVKVARELLKTFGLISNAPTLISCPTCGRLDIDLFSIANEVEEYISNLKVPIKVSVLGC 305 EE+KV RELLK+FGLIS+A TL+SCPTCGRLDIDLFSIANEVEEYIS +KVPIKVSVLGC Sbjct: 241 EEIKVGRELLKSFGLISDAATLVSCPTCGRLDIDLFSIANEVEEYISKIKVPIKVSVLGC 300 Query: 306 AVNGPGEAREADIGIAGARGEGLLFRYGQMIRKVPEAELVSELKKEIDAIVVHFEATGEI 365 AVNGPGEAREADIGIAGARGEGLLFRYG+MIRKVPEA +V ELKKEID IV +E TGEI Sbjct: 301 AVNGPGEAREADIGIAGARGEGLLFRYGEMIRKVPEATMVEELKKEIDHIVKVYEETGEI 360 Query: 366 PGRKH 370 PGRKH Sbjct: 361 PGRKH 365 >B1DDU0.1 B1DDU0_9BACL 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase EC=1.17.4.3 Length = 369 Score = 582 bits (1499), Expect = e-164, Method: Compositional matrix adjust. Identities = 294/365 (80%), Positives = 312/365 (85%) Query: 6 MFLRHQTRPXXXXXXXXXXXXEVAIQSMCTTKTADVEATVAEILRLEEAGCQLVRVTVNN 65 M+LR T P EV IQSMCTTKTADVEATVAEILRLEEAGCQ+VRVTVNN Sbjct: 1 MYLRKDTVPVKVGNLTIGGSNEVIIQSMCTTKTADVEATVAEILRLEEAGCQIVRVTVNN 60 Query: 66 XXXXXXXXXXXXRIHIPLVADIHFNYKLALLAIENGIDKVRINPGNIGRRDXXXXXXXXX 125 RIHIPLVADIHF+Y+LAL AIENGIDKVRINPGNIGRR+ Sbjct: 61 KEAADAIKEIKKRIHIPLVADIHFDYRLALQAIENGIDKVRINPGNIGRREKIEAVVKAC 120 Query: 126 XXXGIPIRIGVNAGSLESHLLEKYGYPTPEAMVESALYHIGILEELDFHDIIVSLKASDV 185 GIPIRIGVNAGSLE+HLLEKYGYPTPEAMVESAL+HIGILEELDFHDIIVSLKASDV Sbjct: 121 KERGIPIRIGVNAGSLENHLLEKYGYPTPEAMVESALFHIGILEELDFHDIIVSLKASDV 180 Query: 186 PMAIEAYRKAAEVIPYPLHLGITESGTLFAGTVKSSAGIGALLSMGIGSTVRISLSADPV 245 PMAI AY AAE I YPLHLGITE+GTL +GT+KSSAGIGALL+MGIG+TVRISLSADPV Sbjct: 181 PMAIAAYSMAAEKIKYPLHLGITEAGTLHSGTIKSSAGIGALLAMGIGNTVRISLSADPV 240 Query: 246 EEVKVARELLKTFGLISNAPTLISCPTCGRLDIDLFSIANEVEEYISNLKVPIKVSVLGC 305 EEVKVARELLKTFGLI+NA TLISCPTCGRLDIDLFSIANEVEEYIS LKVPIKVSVLGC Sbjct: 241 EEVKVARELLKTFGLITNAATLISCPTCGRLDIDLFSIANEVEEYISKLKVPIKVSVLGC 300 Query: 306 AVNGPGEAREADIGIAGARGEGLLFRYGQMIRKVPEAELVSELKKEIDAIVVHFEATGEI 365 AVNGPGEAREADIGIAGARGEG+LFRYG+MIRKVPEAEL++ELKKEID IV FEATGEI Sbjct: 301 AVNGPGEAREADIGIAGARGEGMLFRYGEMIRKVPEAELLNELKKEIDEIVRVFEATGEI 360 Query: 366 PGRKH 370 PGR + Sbjct: 361 PGRSN 365 >A3IBE7.1 A3IBE7_9BACI 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase Length = 371 Score = 508 bits (1308), Expect = e-142, Method: Compositional matrix adjust. Identities = 253/352 (71%), Positives = 283/352 (80%) Query: 9 RHQTRPXXXXXXXXXXXXEVAIQSMCTTKTADVEATVAEILRLEEAGCQLVRVTVNNXXX 68 R TRP E+ IQSMCTTKT DV+ATVAEILRLEEAGCQ+VRV V + Sbjct: 7 RTNTRPVRVGNLTIGGSNELFIQSMCTTKTHDVDATVAEILRLEEAGCQIVRVAVPDERA 66 Query: 69 XXXXXXXXXRIHIPLVADIHFNYKLALLAIENGIDKVRINPGNIGRRDXXXXXXXXXXXX 128 RIHIPLVADIHF+Y+LAL AIENGIDKVRINPGNIGR++ Sbjct: 67 ADAIAEIKKRIHIPLVADIHFDYRLALKAIENGIDKVRINPGNIGRKEKVEAVVNAAKAK 126 Query: 129 GIPIRIGVNAGSLESHLLEKYGYPTPEAMVESALYHIGILEELDFHDIIVSLKASDVPMA 188 GIPIRIGVNAGSLE H+LEKYGYPT + MVESAL+HI ILE+LDFHDIIVS+KASDV +A Sbjct: 127 GIPIRIGVNAGSLERHILEKYGYPTADGMVESALHHIKILEDLDFHDIIVSMKASDVNLA 186 Query: 189 IEAYRKAAEVIPYPLHLGITESGTLFAGTVKSSAGIGALLSMGIGSTVRISLSADPVEEV 248 IEAY KAA+ YPLHLGITESGTLFAGTVKS+AG+GA+LS GIG+T+RISLSADPVEE+ Sbjct: 187 IEAYEKAAKAFNYPLHLGITESGTLFAGTVKSAAGLGAILSKGIGNTLRISLSADPVEEI 246 Query: 249 KVARELLKTFGLISNAPTLISCPTCGRLDIDLFSIANEVEEYISNLKVPIKVSVLGCAVN 308 KVARELLK+FGL SN TLISCPTCGR++IDL SIANEVEEYIS LKVP+KV+VLGCAVN Sbjct: 247 KVARELLKSFGLASNMATLISCPTCGRIEIDLISIANEVEEYISKLKVPLKVAVLGCAVN 306 Query: 309 GPGEAREADIGIAGARGEGLLFRYGQMIRKVPEAELVSELKKEIDAIVVHFE 360 GPGEAREADIGIAGARGEGLLF G+ +RKVPE +V ELKKEID + E Sbjct: 307 GPGEAREADIGIAGARGEGLLFMKGKTVRKVPEETMVEELKKEIDKLAAEME 358 >B1HSS4.1 ISPG_LYSSC 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase EC=1.17.7.1 Length = 371 Score = 507 bits (1306), Expect = e-142, Method: Compositional matrix adjust. Identities = 253/352 (71%), Positives = 282/352 (80%) Query: 9 RHQTRPXXXXXXXXXXXXEVAIQSMCTTKTADVEATVAEILRLEEAGCQLVRVTVNNXXX 68 R TRP E+ IQSMCTTKT DVEATVAEILRLEEAGCQ+VRV V + Sbjct: 7 RSNTRPVRVGNLTIGGSNELFIQSMCTTKTHDVEATVAEILRLEEAGCQIVRVAVPDERA 66 Query: 69 XXXXXXXXXRIHIPLVADIHFNYKLALLAIENGIDKVRINPGNIGRRDXXXXXXXXXXXX 128 RIHIPLVADIHF+Y+LAL AIENGIDKVRINPGNIGR++ Sbjct: 67 ADAIAEIKKRIHIPLVADIHFDYRLALKAIENGIDKVRINPGNIGRKEKVEAVVNAAKAK 126 Query: 129 GIPIRIGVNAGSLESHLLEKYGYPTPEAMVESALYHIGILEELDFHDIIVSLKASDVPMA 188 GIPIRIGVNAGSLE H+LEKYGYPT + MVESAL+HI ILE+LDFHDIIVS+KASDV +A Sbjct: 127 GIPIRIGVNAGSLERHILEKYGYPTADGMVESALHHIKILEDLDFHDIIVSMKASDVNLA 186 Query: 189 IEAYRKAAEVIPYPLHLGITESGTLFAGTVKSSAGIGALLSMGIGSTVRISLSADPVEEV 248 IEAY KAA+ YPLHLGITESGTLFAGTVKS+AG+GA+LS GIG+T+RISLSADPVEE+ Sbjct: 187 IEAYEKAAKAFNYPLHLGITESGTLFAGTVKSAAGLGAILSKGIGNTLRISLSADPVEEI 246 Query: 249 KVARELLKTFGLISNAPTLISCPTCGRLDIDLFSIANEVEEYISNLKVPIKVSVLGCAVN 308 KVARELLK+FGL SN TLISCPTCGR++IDL SIANEVEEYIS L VP+KV+VLGCAVN Sbjct: 247 KVARELLKSFGLASNMATLISCPTCGRIEIDLISIANEVEEYISKLNVPLKVAVLGCAVN 306 Query: 309 GPGEAREADIGIAGARGEGLLFRYGQMIRKVPEAELVSELKKEIDAIVVHFE 360 GPGEAREADIGIAGARGEGLLF G+ +RKVPE +V ELKKEID + E Sbjct: 307 GPGEAREADIGIAGARGEGLLFMKGKTVRKVPEETMVEELKKEIDKLAAEME 358 >A9VH61.1 ISPG_BACWK 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase EC=1.17.7.1 Length = 365 Score = 506 bits (1304), Expect = e-141, Method: Compositional matrix adjust. Identities = 258/359 (71%), Positives = 284/359 (79%) Query: 6 MFLRHQTRPXXXXXXXXXXXXEVAIQSMCTTKTADVEATVAEILRLEEAGCQLVRVTVNN 65 M R +TRP E+ IQSM TTKT DVEATVAEI RLEEAGCQ+VRV V + Sbjct: 1 MTQRTKTRPVKVGNLTIGGNNELIIQSMTTTKTHDVEATVAEIKRLEEAGCQVVRVAVPD 60 Query: 66 XXXXXXXXXXXXRIHIPLVADIHFNYKLALLAIENGIDKVRINPGNIGRRDXXXXXXXXX 125 +I+IPLVADIHF+Y+LAL AIE GIDKVRINPGNIGRR Sbjct: 61 ERAANAIADIKKQINIPLVADIHFDYRLALKAIEGGIDKVRINPGNIGRRHKVEAVVNAA 120 Query: 126 XXXGIPIRIGVNAGSLESHLLEKYGYPTPEAMVESALYHIGILEELDFHDIIVSLKASDV 185 GIPIRIGVNAGSLE H+LEKYGYPT + MVESAL+HI ILE+LDFHDIIVS+KASDV Sbjct: 121 KERGIPIRIGVNAGSLERHILEKYGYPTADGMVESALHHIKILEDLDFHDIIVSMKASDV 180 Query: 186 PMAIEAYRKAAEVIPYPLHLGITESGTLFAGTVKSSAGIGALLSMGIGSTVRISLSADPV 245 +AIEAY KAA YPLHLGITESGTLFAGTVKS+AG+GA+LS GIG+T+RISLSADPV Sbjct: 181 NLAIEAYEKAARAFDYPLHLGITESGTLFAGTVKSAAGLGAILSKGIGNTLRISLSADPV 240 Query: 246 EEVKVARELLKTFGLISNAPTLISCPTCGRLDIDLFSIANEVEEYISNLKVPIKVSVLGC 305 EEVKVARELLK+FGL SNA TLISCPTCGR++IDL SIANEVEEYIS LKVPIKV+VLGC Sbjct: 241 EEVKVARELLKSFGLASNAATLISCPTCGRIEIDLISIANEVEEYISTLKVPIKVAVLGC 300 Query: 306 AVNGPGEAREADIGIAGARGEGLLFRYGQMIRKVPEAELVSELKKEIDAIVVHFEATGE 364 AVNGPGEAREADIGIAGARGEGLLFR GQ++RKVPE +V ELKKEID I A E Sbjct: 301 AVNGPGEAREADIGIAGARGEGLLFRKGQVVRKVPEETMVEELKKEIDVIAAEMAAERE 359 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.137 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,910,285,677 Number of extensions: 71547021 Number of successful extensions: 199660 Number of sequences better than 1.0e-05: 1287 Number of HSP's gapped: 196733 Number of HSP's successfully gapped: 1531 Length of query: 384 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 247 Effective length of database: 1,770,017,148 Effective search space: 437194235556 Effective search space used: 437194235556 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)