BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0422.0008.p (667 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1D9M1.1 B1D9M1_9BACL S-layer domain protein 357 2e-96 B1D995.1 B1D995_9BACL S-layer domain protein 143 7e-32 B1D5Y9.1 B1D5Y9_9BACL Coagulation factor 5/8 type domain protein 141 2e-31 Q2BFV1.1 Q2BFV1_9BACI Truncated lactocepin 141 2e-31 B1D599.1 B1D599_9BACL S-layer domain protein 140 5e-31 >B1D9M1.1 B1D9M1_9BACL S-layer domain protein Length = 709 Score = 357 bits (916), Expect = 2e-96, Method: Compositional matrix adjust. Identities = 182/324 (56%), Positives = 236/324 (72%), Gaps = 3/324 (0%) Query: 346 IEVPLSKVIIEAAKAKGIANIAVTFNGLTVTIPVS-QFSDAVTLTVTNEDDATVTSLTSL 404 +++ L+ +I+ A G+ I +T NGL+ +PV QF+ AV + N+ A ++ L Sbjct: 387 VKIGLNNALIQEAIKAGLGGINLTVNGLSAVLPVGGQFNGAVDFNI-NKSKADSSTTGEL 445 Query: 405 KLASKVYSFDLTVGGVKTSTFKQPLTIKLPLANTTGLDKELLSVSKVVYKTLEFHGGVVD 464 K AS VYSF LT+GG ++F QP+T+ +PL N G+DKELLS++K+V L F GG V+ Sbjct: 446 KAASDVYSFGLTIGGQSVTSFAQPVTVAIPLGNVDGVDKELLSLAKIVDGKLVFQGGTVE 505 Query: 465 GDYIVEPRDTFSTYAVVENKVNFNDVASVQAWAGRQIQVVAAKGAIEGVGASKFAPKSNV 524 G+ IVE RDTFS Y VVENKV+F D+ASVQ+WAGR I+V+AAKGAIEG A FAPK NV Sbjct: 506 GNLIVEHRDTFSQYVVVENKVSFTDIASVQSWAGRAIEVIAAKGAIEGKKAGVFAPKDNV 565 Query: 525 TRAEFSKMLIRALNLENSTATESFGDVASTAWYAPYVAVAAEKGIVTGRSASQFDPNATI 584 TRAEF+KML RAL+++NS+A E F DV T W APY+ AA+ GI+ GRSA++FDPNA I Sbjct: 566 TRAEFAKMLTRALDIDNSSAKEDFADVKDTDWSAPYIGAAAKLGIIQGRSATKFDPNAQI 625 Query: 585 TRAEMATMIARAVK-TVNPQATAGDASSLTKFADAAKIAASLKDGVAFAASHNLVIGNAG 643 TRAEMATMIARA+K T Q ++L F DA+ I+ASLK GVAFA+ + +VIGN G Sbjct: 626 TRAEMATMIARALKVTKGAQDVTDVTAALASFKDASSISASLKAGVAFASKNGIVIGNNG 685 Query: 644 KFNPNNTATRAEAAVIIYRTINFK 667 KF+PN +ATRAEAAV+IYR +NFK Sbjct: 686 KFSPNASATRAEAAVMIYRAVNFK 709 Score = 57.0 bits (136), Expect = 7e-06, Method: Compositional matrix adjust. Identities = 67/215 (31%), Positives = 101/215 (46%), Gaps = 20/215 (9%) Query: 1 VSLKKKLAVSTLAASMTAASFAGFPFSSTGLAQHLGLVGTAAAAEVGHQTVKDKITTIYK 60 +++KKK+ VST+AASM A +FAG P SS GLA+ LG TA AA + + + Sbjct: 1 MTIKKKIIVSTIAASMAATAFAGIPLSSKGLAEKLGFSNTAYAATSSTDQLIAEANKWHD 60 Query: 61 NLTVTEATYLKNYSDEVAVLSQPKFEEIFHPVLKNIKLTDTEKATSLKLFKXXXXXXYDV 120 L V+ LK D + L+ + + I PV +I AT L D+ Sbjct: 61 ALGVSGLATLKTLRDVIKGLTPAEVKAIVLPVTSSI-------ATKYSLSTDEKIAIEDL 113 Query: 121 YAN--------DFDVLKGIRYDEANVALFNSIADQAKVPNLSFDDILDFFFATNGVEAEL 172 Y N DF GI+ A + ++AD+A V L+F+D+ + V+ L Sbjct: 114 YINLLGFTYTADFSYFDGIQ-SSAGFDVLRTLADEADVSGLTFEDLAAYVLK---VKTNL 169 Query: 173 RTLLANKSGADLVNLA-DPVYQKTLVKDAVYKVLD 206 L S + + LA +P+ TLV++A+ V D Sbjct: 170 FASLDALSVSQVQALATNPLGVVTLVQNAIQAVPD 204 >B1D995.1 B1D995_9BACL S-layer domain protein Length = 543 Score = 143 bits (360), Expect = 7e-32, Method: Compositional matrix adjust. Identities = 122/351 (34%), Positives = 180/351 (51%), Gaps = 35/351 (9%) Query: 342 NAKVIEVPLSKVIIEAAK------AKGIANIAVTFNGLTVTIPVSQFSD----------- 384 + K IEVP S +I+ K NI +T ++IP F++ Sbjct: 199 SGKSIEVPASATLIKLPANTDELLGKDALNI-ITSGKAELSIPSGVFAELKKTLTAAQLQ 257 Query: 385 --AVTLTVTNEDDATVTSL-TSLKLASKVYSFDLT-VGGVKTS----TFKQPLTIKLPLA 436 ++ LTV + A +L T KL+ +Y L + TS FK+P+T++LP+ Sbjct: 258 GGSIALTVKPVEGAAKPTLGTDTKLSGPIYELKLQWIAADGTSYDLHQFKEPVTLRLPVD 317 Query: 437 NTTGLDKELLSVSKVV-YKTLEFHGGVVDGDYIVEPRDTFSTYAVVENKVNFNDVASVQA 495 +T + +L S+ + EF GG V FS+YA++E NF+DV S Sbjct: 318 AST--NPKLASIFYLPDSGAPEFVGGTYGNGVYVAQLSHFSSYAILEINKNFSDVPS-SY 374 Query: 496 WAGRQIQVVAAKGAIEGVGASKFAPKSNVTRAEFSKMLIRALNLENSTATESFGDVASTA 555 WA I+ + AK + G AS F P +VTRAEF+ +L+RAL L+ + T +F D+ + A Sbjct: 375 WASGVIKELFAKQIVTGTTASTFEPNRSVTRAEFTALLVRALGLK-AEGTPAFTDIPAGA 433 Query: 556 WYAPYVAVAAEKGIVTGRSASQFDPNATITRAEMATMIARAVKTVNPQATAGDASSLTKF 615 WYA VA A + GIV G S++ F P A+ITR EM T+ R + + DA++ F Sbjct: 434 WYAADVATAYKAGIVQGVSSTTFSPGASITRQEMVTLAMRGYELMT--GKPADATA-EPF 490 Query: 616 ADAAKIAASLKDGVAFAASHNLVIGNA-GKFNPNNTATRAEAAVIIYRTIN 665 D A I+A + V AA+ +V G A G+F P T+TRAEAA ++Y IN Sbjct: 491 KDEASISAWALNNVKKAAALGIVQGRAKGEFVPLGTSTRAEAAQMLYGIIN 541 >B1D5Y9.1 B1D5Y9_9BACL Coagulation factor 5/8 type domain protein Length = 2533 Score = 141 bits (356), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 98/254 (38%), Positives = 135/254 (53%), Gaps = 12/254 (4%) Query: 417 VGGVKTSTFKQPLTIKLPLANTTGLDKELLSVSKVVYKTLEFH--GGVVDGDYIVEPRDT 474 +G K + F P+ + L ++ +L + K+ + + G +G + E D Sbjct: 2285 LGLSKLTAFAHPVIVHL--KGDAAINPKLAGIYKLNANSEPVYIGGSYENGQWTFEANDP 2342 Query: 475 FSTYAVVENKVNFNDVASVQAWAGRQIQVVAAKGAIEGVGASKFAPKSNVTRAEFSKMLI 534 S YA++E N+NDV S WA IQ +AAK I G G FAP VTRAEF+ +L+ Sbjct: 2343 GS-YALIETVWNYNDVPS-GYWAKDVIQQLAAKHIINGTGGGSFAPGRAVTRAEFAALLV 2400 Query: 535 RALNLENSTATESFGDVASTAWYAPYVAVAAEKGIVTGRSASQFDPNATITRAEMATMIA 594 RAL LE T F DV+S AWYA VA+A+ GIVTG+ +FDPNA ITR EM TM+ Sbjct: 2401 RALKLEGDGGT-VFADVSSDAWYAEAVAIASNAGIVTGKGGGKFDPNAQITREEMVTMLM 2459 Query: 595 RAVKTVNPQATAGDASSLTKFADAAKIAASLKDGVAFAASHNLVIG-NAGKFNPNNTATR 653 RA DA+ F D I++ + V A+ L+ G +AG F+P ATR Sbjct: 2460 RAFLDKGTAEGNKDAT----FHDQQDISSWASEYVEQASELGLIKGRSAGIFDPRGIATR 2515 Query: 654 AEAAVIIYRTINFK 667 AE+A II+ + K Sbjct: 2516 AESAQIIFNLLGAK 2529 >Q2BFV1.1 Q2BFV1_9BACI Truncated lactocepin Length = 1457 Score = 141 bits (355), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 85/246 (34%), Positives = 136/246 (55%), Gaps = 7/246 (2%) Query: 425 FKQPLTIKLPLANTTGLDKELLSVSKVVYKT--LEFHGGVVDGDYIVEPRDTFSTYAVVE 482 F +P+++ L + DK + + + E+ GG +GD + + FS YAV+E Sbjct: 1211 FSEPVSVSLSIKGAELNDKRKAAAYYLNEQQNKWEYTGGKAEGDSVTFSVNHFSKYAVLE 1270 Query: 483 NKVNFNDVASVQAWAGRQIQVVAAKGAIEGVGASKFAPKSNVTRAEFSKMLIRALNLENS 542 N FND+ + WA +I+V+A++ G A +FAP +TRAEF+ +L+RALN+ Sbjct: 1271 NSKTFNDIKT--HWAKDEIEVLASRSITGGKTADRFAPGDKLTRAEFAVLLVRALNIPTE 1328 Query: 543 TATESFGDV-ASTAWYAPYVAVAAEKGIVTGRSASQFDPNATITRAEMATMIARAVKTVN 601 + F DV A +W ++ A+ GIV G +++P ITR +MA MI R+++ +N Sbjct: 1329 SYRGVFPDVTAKQSWSVLHIEAASRAGIVQGDLKGKYNPGEEITREQMAAMIVRSIRYLN 1388 Query: 602 PQATAGDASSLTKFADAAKIAASLKDGVAFAASHNLVIGNAGK-FNPNNTATRAEAAVII 660 G SS KF D + ++ L++ V+ AA+ +V G AG F P TRAEAAV++ Sbjct: 1389 EDLLDG-VSSEKKFKDQSTVSPGLREAVSQAAALGIVKGKAGGTFAPKADTTRAEAAVML 1447 Query: 661 YRTINF 666 YR ++ Sbjct: 1448 YRQLSL 1453 Score = 58.2 bits (139), Expect = 3e-06, Method: Compositional matrix adjust. Identities = 34/122 (27%), Positives = 63/122 (51%), Gaps = 7/122 (5%) Query: 487 FNDVASVQAWAGRQIQVVAAKGAIEGVGASKFAPKSNVTRAEFSKMLIRALN------LE 540 F DV + Q+W+ I+ + G ++G K+ P +TR + + M++R++ L+ Sbjct: 1334 FPDVTAKQSWSVLHIEAASRAGIVQGDLKGKYNPGEEITREQMAAMIVRSIRYLNEDLLD 1393 Query: 541 NSTATESFGDVAS-TAWYAPYVAVAAEKGIVTGRSASQFDPNATITRAEMATMIARAVKT 599 ++ + F D ++ + V+ AA GIV G++ F P A TRAE A M+ R + Sbjct: 1394 GVSSEKKFKDQSTVSPGLREAVSQAAALGIVKGKAGGTFAPKADTTRAEAAVMLYRQLSL 1453 Query: 600 VN 601 ++ Sbjct: 1454 LD 1455 >B1D599.1 B1D599_9BACL S-layer domain protein Length = 1682 Score = 140 bits (353), Expect = 5e-31, Method: Compositional matrix adjust. Identities = 91/247 (36%), Positives = 138/247 (55%), Gaps = 14/247 (5%) Query: 428 PLTIKLPLANTTGLDKELLSVSKVVYKTLEFHGG---VVDGDY------IVEPRDTFSTY 478 P+T+ +P T EL + +V ++ G V +G Y ++ FS Y Sbjct: 1440 PVTVSIPYTPTA---DELKAPEHIVVFYIDAEGNRIPVPNGHYDAKTGQVIFTTKHFSKY 1496 Query: 479 AVVENKVNFNDVASVQAWAGRQIQVVAAKGAIEGVGASKFAPKSNVTRAEFSKMLIRALN 538 AV N+V+FND++ V AWA I +AAKG I+G F P+S++TRAEF +L+RAL Sbjct: 1497 AVQFNQVSFNDLSGV-AWASEAIDALAAKGVIQGKAEGVFDPRSSITRAEFITLLMRALE 1555 Query: 539 LENSTATESFGDVASTAWYAPYVAVAAEKGIVTGRSASQFDPNATITRAEMATMIARAVK 598 L + A FGDV+ +A+Y +A A GIVTG ++F+PN ITR + M ARA+ Sbjct: 1556 LPQAAAAAGFGDVSESAYYYNALASAKAYGIVTGTGDNRFEPNKQITREDTFVMTARALD 1615 Query: 599 TVNPQATAGDASSLTKFADAAKIAASLKDGVAFAASHNLVIGNAGKFNPNNTATRAEAAV 658 ++ Q A L+ F DA I++ + ++ ++ +V G+A +P + TRAEAAV Sbjct: 1616 ALH-QLDASSNGDLSSFKDADAISSYAQASISALSAAGIVQGDATGIHPKSNLTRAEAAV 1674 Query: 659 IIYRTIN 665 +IYR +N Sbjct: 1675 MIYRVLN 1681 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.129 0.346 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,027,773,470 Number of extensions: 107897316 Number of successful extensions: 364051 Number of sequences better than 1.0e-05: 1487 Number of HSP's gapped: 360121 Number of HSP's successfully gapped: 1805 Length of query: 667 Length of database: 3,060,696,203 Length adjustment: 143 Effective length of query: 524 Effective length of database: 1,713,491,058 Effective search space: 897869314392 Effective search space used: 897869314392 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 135 (56.6 bits)