BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0420.0039.p (225 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q8ESM0.1 Q8ESM0_OCEIH Esterase 139 1e-31 Q2B519.1 Q2B519_9BACI Carboxylesterase 132 2e-29 A6CS98.1 A6CS98_9BACI Putative uncharacterized protein 131 4e-29 C5D4C8.1 C5D4C8_9BACI Carboxylesterase 127 5e-28 B4AIL3.1 B4AIL3_BACPU Carboxylesterase 125 2e-27 >Q8ESM0.1 Q8ESM0_OCEIH Esterase Length = 230 Score = 139 bits (351), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 85/229 (37%), Positives = 120/229 (52%), Gaps = 16/229 (6%) Query: 1 MESCLFIHGFTGGEYEISPLAQFL-ERHDYHARTFTLKGHGGSRNDLLQSDRHDWRQSAX 59 M +CL IHG+TGG YE+ PLA FL E D+H TL GHG + L + +W QSA Sbjct: 1 MIACLIIHGYTGGPYEVDPLADFLRENTDWHVEVPTLPGHG-EQLSLKNASYKNWIQSAE 59 Query: 60 XXXXXXXXXXXXVHLIGFSTGALIASYLSVQYQDCIKSLTLLSTPVFPLNL----LEIAR 115 V++IGFS G +IA+YL+ +YQ I L LL+ L+ L+IA Sbjct: 60 AALEPLQKKYEKVYVIGFSMGGMIAAYLAAKYQ--IDKLVLLAPSGKYLSFKQIGLDIAT 117 Query: 116 TLGSPV--------MVRNYLSKFGTTPAKATREFLRLVRESFNIYPQIKIPTLIVQGRRD 167 +G + + +Y K G P KA EF++LVR + ++ P LI G+ D Sbjct: 118 CIGDGIRGDLSKNKLYNHYKKKLGVVPLKANIEFMKLVRFTRRYLDKVNSPVLIAHGQLD 177 Query: 168 HLVRAKSAAYLQRTIPSGSKRVLMVERSGHMLCHGEHSGQMMNEVLQFM 216 +V K+A YL + I S K V++ ERS H++C GE + V QF+ Sbjct: 178 GMVPYKTAYYLDKEIHSEEKEVVVFERSRHLICLGEDKDTLNGMVYQFL 226 >Q2B519.1 Q2B519_9BACI Carboxylesterase Length = 230 Score = 132 bits (333), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 80/230 (34%), Positives = 117/230 (50%), Gaps = 16/230 (6%) Query: 1 MESCLFIHGFTGGEYEISPLAQFLERHD-YHARTFTLKGHGGSRNDLLQSDRHDWRQSAX 59 M CL IHGFTG +EI PL ++L++H + TL GHG R L + W A Sbjct: 1 MIGCLCIHGFTGAPFEIEPLTEYLKKHTAWEVAVPTLPGHG-DRLQLKGIEYKKWISHAE 59 Query: 60 XXXXXXXXXXXXVHLIGFSTGALIASYLSVQYQDCIKSLTLLSTPVFPLNLLEIARTLGS 119 V++IGFS G +IASYL+V Y +K L LLS + +N ++ + +G Sbjct: 60 DELQKLMEKCDEVYVIGFSMGGIIASYLAVHYP--VKKLILLSAAAYYVNPKQLVKDIGE 117 Query: 120 PV------------MVRNYLSKFGTTPAKATREFLRLVRESFNIYPQIKIPTLIVQGRRD 167 + M Y K TP AT +F RLV I QI+IPTLI QG D Sbjct: 118 MIKDTVKGNLFKNEMFLRYNRKIRETPIAATLQFRRLVSSVRPIINQIRIPTLIAQGEVD 177 Query: 168 HLVRAKSAAYLQRTIPSGSKRVLMVERSGHMLCHGEHSGQMMNEVLQFMR 217 +V +SA YL I + SK+++ ++ S H++CH + ++L+F++ Sbjct: 178 GVVPPRSATYLYENIGASSKKLIFLKESKHLICHSGECEGLFQDILEFLQ 227 >A6CS98.1 A6CS98_9BACI Putative uncharacterized protein Length = 248 Score = 131 bits (330), Expect = 4e-29, Method: Compositional matrix adjust. Identities = 81/229 (35%), Positives = 111/229 (48%), Gaps = 16/229 (6%) Query: 1 MESCLFIHGFTGGEYEISPLAQFLERH-DYHARTFTLKGHGGSRNDLLQSDRHDWRQSAX 59 M CL IHGFTGG YE+ PLA +L+ H D+ TL GHG S DL +W + A Sbjct: 19 MNGCLLIHGFTGGPYEVDPLAHYLKCHTDWKIVVPTLPGHGDSL-DLKGKKADEWVECAE 77 Query: 60 XXXXXXXXXXXXVHLIGFSTGALIASYLSVQYQDCIKSLTLLSTPVF---PLNLLEIART 116 V++IGFS G LIASYL+V+Y + L LLS + P LL R Sbjct: 78 KELEVLLEECDEVYVIGFSMGGLIASYLAVKYP--VNKLVLLSAAAYYVNPKQLLTDIRD 135 Query: 117 LGSPV---------MVRNYLSKFGTTPAKATREFLRLVRESFNIYPQIKIPTLIVQGRRD 167 + V + Y K TP AT +F R+V + + IK+P I QG++D Sbjct: 136 MIQDVFRGELSNNELYLRYKKKIKDTPISATYQFRRVVLMAKPLLKDIKVPVFIAQGKKD 195 Query: 168 HLVRAKSAAYLQRTIPSGSKRVLMVERSGHMLCHGEHSGQMMNEVLQFM 216 +V KSA +L I K E + H++CH + S + +V F+ Sbjct: 196 GIVPPKSADFLYEEIAYQEKEKYFSEEAKHLICHSDDSDNLFEKVKDFL 244 >C5D4C8.1 C5D4C8_9BACI Carboxylesterase Length = 238 Score = 127 bits (320), Expect = 5e-28, Method: Compositional matrix adjust. Identities = 83/233 (35%), Positives = 121/233 (51%), Gaps = 18/233 (7%) Query: 1 MESCLFIHGFTGGEYEISPLAQFLE-RHDYHARTFTLKGHGGSRNDLLQSDRHDWRQSAX 59 M CL IHGFTG E++PLA++L+ R D+ +T L GHG R L W Q+A Sbjct: 3 MIGCLCIHGFTGSPDEVAPLAEYLQKRTDWVIKTPILPGHG-KRLQLKGITYDQWFQAAE 61 Query: 60 XXXXXXXXXXXXVHLIGFSTGALIASYLSVQYQDCIKSLTLLSTPVFPLNLLEIARTL-- 117 V++IGFS G +IA YL+ +Y+ I L LLS + +N ++ + + Sbjct: 62 RELQALMEICETVYVIGFSMGGVIAVYLAEKYK--IDKLVLLSAAFYYVNPRQLIKDIHE 119 Query: 118 -----------GSPVMVRNYLSKFGTTPAKATREFLRLVRESFNIYPQIKIPTLIVQGRR 166 +P+ +R Y +K TP A EF +LV E P + IP LIVQG + Sbjct: 120 IVRDGWRRRLRENPLFIR-YKNKILATPLTAILEFRKLVNEVRPRLPNVHIPALIVQGEK 178 Query: 167 DHLVRAKSAAYLQRTIPSGSKRVLMVERSGHMLCHGEHSGQMMNEVLQFMRTS 219 D +V KSA YL I S K++L+++ S H +CHG + EV +F+R + Sbjct: 179 DGIVPVKSAHYLYNKIGSSQKKLLVLQESYHHVCHGPDRYLLFCEVEKFLREN 231 >B4AIL3.1 B4AIL3_BACPU Carboxylesterase Length = 230 Score = 125 bits (315), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 81/236 (34%), Positives = 122/236 (51%), Gaps = 24/236 (10%) Query: 1 MESCLFIHGFTGGEYEISPLAQFL-ERHDYHARTFTLKGHGGSRNDLLQSD---RHDWRQ 56 M CL IHGFTG YE+ PLAQ+L ++ D++ ++ TL GHG D LQ +W Sbjct: 1 MIGCLCIHGFTGAPYEVEPLAQYLKQKTDWNVQSVTLPGHG----DELQLKGILYQEWIA 56 Query: 57 SAXXXXXXXXXXXXXVHLIGFSTGALIASYLSVQYQDCIKSLTLLSTP---VFPLNLLEI 113 +A + LIGFS G +IASYL+ +Y + L LLS V P +++ Sbjct: 57 TAELELLSLYRTCDTIFLIGFSMGGMIASYLAAKYP--VARLVLLSAAAKYVNPKQMIQD 114 Query: 114 ARTL----------GSPVMVRNYLSKFGTTPAKATREFLRLVRESFNIYPQIKIPTLIVQ 163 R L +P+ R Y K +TP A EF++LV+++ + ++ +P LIVQ Sbjct: 115 TRQLVKESLSGLIDTNPLYDR-YRHKLTSTPLSAAVEFMKLVKQTKSSLEKLHLPVLIVQ 173 Query: 164 GRRDHLVRAKSAAYLQRTIPSGSKRVLMVERSGHMLCHGEHSGQMMNEVLQFMRTS 219 G D +V A SA ++ IPS K V + H +CH + Q+ +V QF++ + Sbjct: 174 GESDGIVPASSAQFIDHKIPSRQKEVFYLPDCKHHVCHEPCAQQLFEKVEQFLKKT 229 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.323 0.137 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,211,158,969 Number of extensions: 43352068 Number of successful extensions: 112369 Number of sequences better than 1.0e-05: 253 Number of HSP's gapped: 112171 Number of HSP's successfully gapped: 253 Length of query: 225 Length of database: 3,060,696,203 Length adjustment: 131 Effective length of query: 94 Effective length of database: 1,826,543,238 Effective search space: 171695064372 Effective search space used: 171695064372 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 129 (54.3 bits)