BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0420.0037.p (565 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q7DI93.1 Q7DI93_THEVU Alpha-amylase TVA II 518 e-145 Q08751.1 NEPU2_THEVU Neopullulanase 2 EC=3.2.1.135 518 e-145 Q5BLZ7.1 Q5BLZ7_THETP Cyclomaltodextrinase EC=3.2.1.54 501 e-140 B0K6K5.1 B0K6K5_THEPX Alpha amylase, catalytic region 435 e-120 P29964.2 CDAS_THEP3 Cyclomaltodextrinase EC=3.2.1.54 432 e-119 >Q7DI93.1 Q7DI93_THEVU Alpha-amylase TVA II Length = 585 Score = 518 bits (1333), Expect = e-145, Method: Compositional matrix adjust. Identities = 259/588 (44%), Positives = 361/588 (61%), Gaps = 39/588 (6%) Query: 6 LYHTAEDPFAFPVGSGLLKLRLFVQNGQQLSCTVIHSDRYDTPGSEIPLPME-RIGSAGA 64 ++H A+ +A+P+ L++RL + G + C V+++DRY +P E+ + + GS Sbjct: 6 IFHEAKGSYAYPISETQLRVRLRAKKGDVVRCEVLYADRYASPEEELAHALAGKAGSDER 65 Query: 65 YEIHEAVLQSATGKCRYLFHVENPAGDYVWYGERGLSENREHAGSFQYAYMHRPEAVKLP 124 ++ EA+L+ +T + +Y+F + P G+ V++GE G S R AG FQYAY+HR E P Sbjct: 66 FDYFEALLECSTKRVKYVFLLTGPQGEAVYFGETGFSAERSKAGVFQYAYIHRSEVFTTP 125 Query: 125 SWSSDAVVYQIFP--------------------------NSYNGGTLKGITEKIPYLHQL 158 W+ +AV+YQIFP +S+ GG LKG+ +++PYL +L Sbjct: 126 EWAKEAVIYQIFPERFANGDPSNDPPGTEQWAKDARPRHDSFYGGDLKGVIDRLPYLEEL 185 Query: 159 GITALYMTPIFDSPSAHKYNTSDYYKVDPAFGSTEDLKALVDEAHRHGIRVLLDAVFNHS 218 G+TALY TPIF SPS HKY+T+DY +DP FG + LVDEAHR GI+++LDAVFNH+ Sbjct: 186 GVTALYFTPIFASPSHHKYDTADYLAIDPQFGDLPTFRRLVDEAHRRGIKIILDAVFNHA 245 Query: 219 GDAFFAFKDVLEHGENSRYKDWFVIHSFPVVQTPSPNYETFAKAEAHMPKLNMDNPDVAD 278 GD FFAF+DVL+ GE SRYKDWF I FPV +T NYETFA MPKL +NP+V + Sbjct: 246 GDQFFAFRDVLQKGEQSRYKDWFFIEDFPVSKTSRTNYETFAVQVPAMPKLRTENPEVKE 305 Query: 279 YMMEVSKHWVREAGIDGWRLDVANEVSPRFWTRFRRELKAEFPELLLIGEIMHDSGPWLR 338 Y+ +V++ W+ E GIDGWRLDVANEV FW FRR +K+ P+ L++GEI HD+ WL Sbjct: 306 YLFDVARFWM-EQGIDGWRLDVANEVDHAFWREFRRLVKSLNPDALIVGEIWHDASGWLM 364 Query: 339 GDQFDGGMNYVLRDAVLEFFAEQYAGPVRFMEQLLHQEALCNDQANSAMFQLLGSHDTLR 398 GDQFD MNY+ R++V+ FFA RF +L L +QA ++ LL SHDT R Sbjct: 365 GDQFDSVMNYLFRESVIRFFATGEIHAERFDAELTRARMLYPEQAAQGLWNLLDSHDTER 424 Query: 399 FLTACKQGGRGWDHENTAVQRMRLAVFFQMTYIGMPMIYYGDEVGMEGATDPHCRLPMVW 458 FLT+C GG N A + RLAV FQMTY+G P+IYYGDE+GM GATDP CR PM+W Sbjct: 425 FLTSC--GG------NEA--KFRLAVLFQMTYLGTPLIYYGDEIGMAGATDPDCRRPMIW 474 Query: 459 KEQEQNTALHAWYTQLISLRKQYEVLRKGAFRPWITDEARNVLGYIRCSGQDQMGLLINN 518 +E+EQN L +Y +LI LR + L +G R W D+ N+ ++R +G+++NN Sbjct: 475 EEKEQNRGLFEFYKELIRLRHRLASLTRGNVRSWHADKQANLYAFVRTVQDQHVGVVLNN 534 Query: 519 SPNRYQLELSRFRSDANVLTDLLTGATFPGT-GDFLVEIEPFGCLMLY 565 + + L S D LTG G G + + P+ ++L+ Sbjct: 535 RGEKQTVLLQVPESGGKTWLDCLTGEEVHGKQGQLKLTLRPYQGMILW 582 >Q08751.1 NEPU2_THEVU Neopullulanase 2 EC=3.2.1.135 Length = 585 Score = 518 bits (1333), Expect = e-145, Method: Compositional matrix adjust. Identities = 259/588 (44%), Positives = 361/588 (61%), Gaps = 39/588 (6%) Query: 6 LYHTAEDPFAFPVGSGLLKLRLFVQNGQQLSCTVIHSDRYDTPGSEIPLPME-RIGSAGA 64 ++H A+ +A+P+ L++RL + G + C V+++DRY +P E+ + + GS Sbjct: 6 IFHEAKGSYAYPISETQLRVRLRAKKGDVVRCEVLYADRYASPEEELAHALAGKAGSDER 65 Query: 65 YEIHEAVLQSATGKCRYLFHVENPAGDYVWYGERGLSENREHAGSFQYAYMHRPEAVKLP 124 ++ EA+L+ +T + +Y+F + P G+ V++GE G S R AG FQYAY+HR E P Sbjct: 66 FDYFEALLECSTKRVKYVFLLTGPQGEAVYFGETGFSAERSKAGVFQYAYIHRSEVFTTP 125 Query: 125 SWSSDAVVYQIFP--------------------------NSYNGGTLKGITEKIPYLHQL 158 W+ +AV+YQIFP +S+ GG LKG+ +++PYL +L Sbjct: 126 EWAKEAVIYQIFPERFANGDPSNDPPGTEQWAKDARPRHDSFYGGDLKGVIDRLPYLEEL 185 Query: 159 GITALYMTPIFDSPSAHKYNTSDYYKVDPAFGSTEDLKALVDEAHRHGIRVLLDAVFNHS 218 G+TALY TPIF SPS HKY+T+DY +DP FG + LVDEAHR GI+++LDAVFNH+ Sbjct: 186 GVTALYFTPIFASPSHHKYDTADYLAIDPQFGDLPTFRRLVDEAHRRGIKIILDAVFNHA 245 Query: 219 GDAFFAFKDVLEHGENSRYKDWFVIHSFPVVQTPSPNYETFAKAEAHMPKLNMDNPDVAD 278 GD FFAF+DVL+ GE SRYKDWF I FPV +T NYETFA MPKL +NP+V + Sbjct: 246 GDQFFAFRDVLQKGEQSRYKDWFFIEDFPVSKTSRTNYETFAVQVPAMPKLRTENPEVKE 305 Query: 279 YMMEVSKHWVREAGIDGWRLDVANEVSPRFWTRFRRELKAEFPELLLIGEIMHDSGPWLR 338 Y+ +V++ W+ E GIDGWRLDVANEV FW FRR +K+ P+ L++GEI HD+ WL Sbjct: 306 YLFDVARFWM-EQGIDGWRLDVANEVDHAFWREFRRLVKSLNPDALIVGEIWHDASGWLM 364 Query: 339 GDQFDGGMNYVLRDAVLEFFAEQYAGPVRFMEQLLHQEALCNDQANSAMFQLLGSHDTLR 398 GDQFD MNY+ R++V+ FFA RF +L L +QA ++ LL SHDT R Sbjct: 365 GDQFDSVMNYLFRESVIRFFATGEIHAERFDAELTRARMLYPEQAAQGLWNLLDSHDTER 424 Query: 399 FLTACKQGGRGWDHENTAVQRMRLAVFFQMTYIGMPMIYYGDEVGMEGATDPHCRLPMVW 458 FLT+C GG N A + RLAV FQMTY+G P+IYYGDE+GM GATDP CR PM+W Sbjct: 425 FLTSC--GG------NEA--KFRLAVLFQMTYLGTPLIYYGDEIGMAGATDPDCRRPMIW 474 Query: 459 KEQEQNTALHAWYTQLISLRKQYEVLRKGAFRPWITDEARNVLGYIRCSGQDQMGLLINN 518 +E+EQN L +Y +LI LR + L +G R W D+ N+ ++R +G+++NN Sbjct: 475 EEKEQNRGLFEFYKELIRLRHRLASLTRGNVRSWHADKQANLYAFVRTVQDQHVGVVLNN 534 Query: 519 SPNRYQLELSRFRSDANVLTDLLTGATFPGT-GDFLVEIEPFGCLMLY 565 + + L S D LTG G G + + P+ ++L+ Sbjct: 535 RGEKQTVLLQVPESGGKTWLDCLTGEEVHGKQGQLKLTLRPYQGMILW 582 >Q5BLZ7.1 Q5BLZ7_THETP Cyclomaltodextrinase EC=3.2.1.54 Length = 582 Score = 501 bits (1290), Expect = e-140, Method: Compositional matrix adjust. Identities = 258/587 (43%), Positives = 352/587 (59%), Gaps = 39/587 (6%) Query: 6 LYHTAEDPFAFPVGSGLLKLRLFVQNGQQLSCTVIHSDRYDTPGSEIP-LPMERIGSAGA 64 L+H P+A P L++RL G+ C V+H+DRY+ S E + S Sbjct: 6 LFHETRVPYACPENLHSLRVRLRAGKGEIARCLVLHADRYELLDSCFQQTEAEWMASDQR 65 Query: 65 YEIHEAVLQSATGKCRYLFHVENPAGDYVWYGERGLSENREHAGSFQYAYMHRPEAVKLP 124 ++ EA+L+ + +Y+F +E G+ V++GE G+S+ R AG FQYAY+H P Sbjct: 66 FDYFEALLECTAKRLQYVFLLEGKKGERVYFGESGVSKERNRAGVFQYAYIHESALFVTP 125 Query: 125 SWSSDAVVYQIFPN--------------------------SYNGGTLKGITEKIPYLHQL 158 W+ DAVVYQIFP+ S+ GG L+G+ +PYL +L Sbjct: 126 EWARDAVVYQIFPDRFANGDKENDPPRTEDWSKDARPGRDSFYGGDLEGVIHSLPYLRKL 185 Query: 159 GITALYMTPIFDSPSAHKYNTSDYYKVDPAFGSTEDLKALVDEAHRHGIRVLLDAVFNHS 218 G+ ALY TPIF SPS HKY+T+DY ++DP FG+ + K L+ EAHRHG+R++LDAVFNHS Sbjct: 186 GVNALYFTPIFASPSNHKYDTADYLRIDPQFGNLDTFKRLIREAHRHGMRIILDAVFNHS 245 Query: 219 GDAFFAFKDVLEHGENSRYKDWFVIHSFPVVQTPSPNYETFAKAEAHMPKLNMDNPDVAD 278 GD FFAF+DVLE G +S+Y+DWF I FPVVQTP PNYETFA MPKL +N +V + Sbjct: 246 GDRFFAFRDVLEKGADSQYRDWFFIEQFPVVQTPQPNYETFAVNVPVMPKLRTENREVKE 305 Query: 279 YMMEVSKHWVREAGIDGWRLDVANEVSPRFWTRFRRELKAEFPELLLIGEIMHDSGPWLR 338 Y+ +V ++W E GIDGWRLDVANEV FW FR +K PE L+IGE+ H++ WL Sbjct: 306 YLFQVVRYWT-EMGIDGWRLDVANEVDHAFWRDFRSFVKMLNPEALIIGEVWHNAVKWLE 364 Query: 339 GDQFDGGMNYVLRDAVLEFFAEQYAGPVRFMEQLLHQEALCNDQANSAMFQLLGSHDTLR 398 GDQFD MNY+ RD V FFAEQ + F +L DQA + LL SHDT R Sbjct: 365 GDQFDSVMNYLFRDCVYRFFAEQTSDASEFDAELTTARISYPDQALQVAWNLLDSHDTER 424 Query: 399 FLTACKQGGRGWDHENTAVQRMRLAVFFQMTYIGMPMIYYGDEVGMEGATDPHCRLPMVW 458 FLT+C + +R RLAV FQMTY+G P+IYYGDE+GM G TDP CR PM+W Sbjct: 425 FLTSCGEKE----------ERFRLAVLFQMTYVGTPLIYYGDEIGMTGETDPDCRRPMLW 474 Query: 459 KEQEQNTALHAWYTQLISLRKQYEVLRKGAFRPWITDEARNVLGYIRCSGQDQMGLLINN 518 ++EQN AL ++Y +LI +R+ LR+G+FR W+ D V ++R + G+++NN Sbjct: 475 DKEEQNQALFSYYQRLIRIRRSCAPLRRGSFRTWLADPGSPVYAFVRSWQGENAGIVLNN 534 Query: 519 SPNRYQLELSRFRSDANVLTDLLTGATFPGTGDFL-VEIEPFGCLML 564 S + + L TDLLTG +G+ + + + P+ ++L Sbjct: 535 SDSWQNVSLPLDTWAKRNWTDLLTGEKGQASGNRMELILSPYQGIVL 581 >B0K6K5.1 B0K6K5_THEPX Alpha amylase, catalytic region Length = 574 Score = 435 bits (1118), Expect = e-120, Method: Compositional matrix adjust. Identities = 224/564 (39%), Positives = 325/564 (57%), Gaps = 40/564 (7%) Query: 1 MIRWFLYHTAEDPFAFPVGSGLLKLRLFVQNGQQLSCTVIHSDRYDTPGSEIPLPMERIG 60 MI+ ++H ++ P+A+P+ LK+ L V++ DRYD G PM Sbjct: 1 MIKEAIFHKSDMPYAYPLNENQLKIVLRTAVFDVDRVYVLYKDRYDWLGKFKIKPMVLTH 60 Query: 61 SAGAYEIHEAVLQSATGKCRYLFHVENPAGDYVWYGERGLSENREHA---GSFQYAYMHR 117 + ++ +E L+ K Y F++ + G+ ++Y E G + R G F Y Y+ Sbjct: 61 TNELFDYYETTLE-LNKKFVYFFYLVSDGGEKLYYTEAGFYKKRPENHFWGFFHYPYIGE 119 Query: 118 PEAVKLPSWSSDAVVYQIFPNSYN------------------------GGTLKGITEKIP 153 + P W+SD +VYQIFP +N GG L+GI +KI Sbjct: 120 KDVFFAPEWTSDCIVYQIFPERFNNGDKSNDPESVKPWGEKPTADSFFGGDLQGIIDKID 179 Query: 154 YLHQLGITALYMTPIFDSPSAHKYNTSDYYKVDPAFGSTEDLKALVDEAHRHGIRVLLDA 213 YL LGI A+Y+TPIF SPS HKY+T+DYY +DP FG T+ + LV + H +GI+V+ DA Sbjct: 180 YLKDLGINAIYLTPIFLSPSTHKYDTTDYYTIDPHFGDTQKARELVQKCHDNGIKVIFDA 239 Query: 214 VFNHSGDAFFAFKDVLEHGENSRYKDWFVIHSFPVVQTPSPNYETFAKAEAHMPKLNMDN 273 VFNH G FFAF+DV+++G+ S+Y +WF I+ P+ P P+YE FA MPKL N Sbjct: 240 VFNHCGYDFFAFQDVIKNGKKSKYWNWFNIYECPLKTHPKPSYEAFADTVWRMPKLMTKN 299 Query: 274 PDVADYMMEVSKHWVREAGIDGWRLDVANEVSPRFWTRFRRELKAEFPELLLIGEIMHDS 333 P+V Y++EV+++W++E IDGWRLDVANE+ FW +FR +KA PE +++GE+ HD+ Sbjct: 300 PEVQKYLLEVAEYWIKEVDIDGWRLDVANEIDHHFWRKFREVVKAAKPEAIIVGEVWHDA 359 Query: 334 GPWLRGDQFDGGMNYVLRDAVLEFFAEQYAGPVRFMEQLLHQEALCNDQANSAMFQLLGS 393 PWLRGDQFD MNY R+AV++FFAE+ RF + Q D N MF L+GS Sbjct: 360 SPWLRGDQFDSVMNYPFRNAVVDFFAERKISASRFNTMITEQLMRHMDSVNRVMFNLIGS 419 Query: 394 HDTLRFLTACKQGGRGWDHENTAVQRMRLAVFFQMTYIGMPMIYYGDEVGMEGATDPHCR 453 HDT RFLT N V RM+LA+ FQ T++G+P IYYGDEVGM G DP CR Sbjct: 420 HDTERFLTLA----------NGMVARMKLALVFQFTFVGIPYIYYGDEVGMVGGYDPDCR 469 Query: 454 LPMVWKEQEQNTALHAWYTQLISLRKQYEVLRKGAFRPWITDEARNVLGYIRCSGQDQMG 513 M+W+E++QN ++ +Y +LIS+R++ E L+ G+F V + R + Sbjct: 470 RCMIWEEEKQNKSIFNFYKKLISIRRENEELKYGSFCTLYA--IGRVFAFKREYKGKSII 527 Query: 514 LLINNSPNRYQLELSRFRSDANVL 537 +++NNS + + L+ ++L Sbjct: 528 VVLNNSSKQEVIFLNEVEGKEDIL 551 >P29964.2 CDAS_THEP3 Cyclomaltodextrinase EC=3.2.1.54 Length = 574 Score = 432 bits (1110), Expect = e-119, Method: Compositional matrix adjust. Identities = 223/564 (39%), Positives = 325/564 (57%), Gaps = 40/564 (7%) Query: 1 MIRWFLYHTAEDPFAFPVGSGLLKLRLFVQNGQQLSCTVIHSDRYDTPGSEIPLPMERIG 60 MI+ ++H ++ P+A+P+ LK+ L V++ DRYD G PM Sbjct: 1 MIKEAIFHKSDVPYAYPLNENQLKIVLRTAVFDVDRVYVLYKDRYDWLGKFKIKPMVLTH 60 Query: 61 SAGAYEIHEAVLQSATGKCRYLFHVENPAGDYVWYGERGLSENREHA---GSFQYAYMHR 117 + ++ +E L+ K Y F++ + G+ ++Y E G + R G F Y Y+ Sbjct: 61 TNELFDYYETTLE-LNKKFVYFFYLVSDGGEKLYYTEAGFYKKRPENHFWGFFHYPYIGE 119 Query: 118 PEAVKLPSWSSDAVVYQIFPNSYN------------------------GGTLKGITEKIP 153 + P W+SD +VYQIFP +N GG L+GI +KI Sbjct: 120 KDVFFAPEWTSDCMVYQIFPERFNNGDKSNDPENVKPWGEKPTADSFFGGDLQGIIDKID 179 Query: 154 YLHQLGITALYMTPIFDSPSAHKYNTSDYYKVDPAFGSTEDLKALVDEAHRHGIRVLLDA 213 YL LGI A+Y+TPIF S S HKY+T+DYY +DP FG T+ + LV + H +GI+V+ DA Sbjct: 180 YLKDLGINAIYLTPIFLSHSTHKYDTTDYYTIDPHFGDTQKARELVQKCHDNGIKVIFDA 239 Query: 214 VFNHSGDAFFAFKDVLEHGENSRYKDWFVIHSFPVVQTPSPNYETFAKAEAHMPKLNMDN 273 VFNH G FFAF+DV+++G+ S+Y DWF I+ +P+ P P+YE FA MPKL N Sbjct: 240 VFNHCGYDFFAFQDVIKNGKKSKYWDWFNIYEWPIKTHPKPSYEAFADTVWRMPKLMTKN 299 Query: 274 PDVADYMMEVSKHWVREAGIDGWRLDVANEVSPRFWTRFRRELKAEFPELLLIGEIMHDS 333 P+V Y++EV+++W++E IDGWRLDVANE+ FW +FR +KA PE +++GE+ HD+ Sbjct: 300 PEVQKYLLEVAEYWIKEVDIDGWRLDVANEIDHHFWRKFREVVKAAKPEAIIVGEVWHDA 359 Query: 334 GPWLRGDQFDGGMNYVLRDAVLEFFAEQYAGPVRFMEQLLHQEALCNDQANSAMFQLLGS 393 PWLRGDQFD MNY R+AV++FFA++ RF + Q D N MF L+GS Sbjct: 360 SPWLRGDQFDSVMNYPFRNAVVDFFAKRKISASRFNTMITEQLMRHMDSVNRVMFNLIGS 419 Query: 394 HDTLRFLTACKQGGRGWDHENTAVQRMRLAVFFQMTYIGMPMIYYGDEVGMEGATDPHCR 453 HDT RFLT N V RM+LA+ FQ T++G+P IYYGDEVGM G DP CR Sbjct: 420 HDTERFLTLA----------NGMVARMKLALVFQFTFVGIPYIYYGDEVGMVGDYDPDCR 469 Query: 454 LPMVWKEQEQNTALHAWYTQLISLRKQYEVLRKGAFRPWITDEARNVLGYIRCSGQDQMG 513 M+W+E++QN ++ +Y +LIS+R++ E L+ G+F V + R + Sbjct: 470 RCMIWEEEKQNKSIFNFYKKLISIRRENEELKYGSFCTLYA--IGRVFAFKREYKGKSII 527 Query: 514 LLINNSPNRYQLELSRFRSDANVL 537 +++NNS + + L+ ++L Sbjct: 528 VVLNNSSKQEVIFLNEVEGKEDIL 551 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.138 0.437 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,669,155,120 Number of extensions: 159426258 Number of successful extensions: 325941 Number of sequences better than 1.0e-05: 4862 Number of HSP's gapped: 315827 Number of HSP's successfully gapped: 5046 Length of query: 565 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 424 Effective length of database: 1,732,333,088 Effective search space: 734509229312 Effective search space used: 734509229312 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 134 (56.2 bits)