BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0420.0031.p (355 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DFD0.1 B1DFD0_9BACL Peptidase M23B 404 e-110 B3KKR5.1 B3KKR5_9BACI Peptidase M23B 400 e-109 Q65FB3.1 Q65FB3_BACLD YunA 340 2e-91 A7Z8E9.1 A7Z8E9_BACA2 YunA 332 4e-89 A8FH35.1 A8FH35_BACP2 M23B subfamily peptidase 332 5e-89 >B1DFD0.1 B1DFD0_9BACL Peptidase M23B Length = 356 Score = 404 bits (1037), Expect = e-110, Method: Compositional matrix adjust. Identities = 194/356 (54%), Positives = 243/356 (68%), Gaps = 8/356 (2%) Query: 1 MLALFKIAAIRKTVLCLSAAAMLWGDSDAVAGAPAGNKHAVSSEAASGKESLSVSRKQLY 60 M F IA L + + + + A AGN ++++ G+ SR+QLY Sbjct: 1 MKQTFSIALAAVVASMLISGQTGYANPNQNANTNAGNTKPAQTQSSEGEVDPLTSRQQLY 60 Query: 61 DQMSAATGIAWFRFAAIDQYERTIAKKKSGTAGQAQPRL---TGISFPPPVWSGPLNPNQ 117 D++S TGI W+R AA+DQYERT++K + +A+P+L GI W+GPLNP+ Sbjct: 61 DRLSTVTGIPWYRIAAVDQYERTLSKARP----KARPQLGGIVGIYIDEEQWTGPLNPDS 116 Query: 118 EDQSPESIRFFXXXXXXXXXXXXADPENDADVLYSMARHLMKYGDSS-SDFNIGVWEYYH 176 D P SI++F AD ND D+LYS+A L++YG S+ DF+IGVWEYYH Sbjct: 117 SDNVPASIQWFNGVGRDGDGDGFADRNNDLDLLYSVANRLLEYGGSTEDDFSIGVWEYYH 176 Query: 177 NGRATQRIEQFARLYEHFGRLDLSGSAFPLPVGKTYSYRSTWGTGRSWGGARIHEGTDLF 236 N RA QRI+QF+ +Y FGRLDL AFP+PVG YSYRSTWG R WGG R HEGTD+F Sbjct: 177 NSRAVQRIQQFSNIYATFGRLDLYEHAFPVPVGTNYSYRSTWGNARGWGGRRTHEGTDIF 236 Query: 237 TPYGMTVRSTCFGIVETKGWNRYGGWRIGIRDIENRYHYYAHLAGFEKSLSRGDIVAPGQ 296 +G+ VRSTCFG+VE KGWN YGGWRIGIRD+ N YHYYAHL+GF+K++ G IV PGQ Sbjct: 237 ANHGIPVRSTCFGVVEIKGWNPYGGWRIGIRDLNNYYHYYAHLSGFDKTIGIGTIVKPGQ 296 Query: 297 TIGWVGSSGYGNPGTQGKFPPHLHYGIYRDRGITEWAFDPYPLLKQWETQEYRAMR 352 TIGWVGSSGYG PGTQGKFPPHLHYG+YRDRG+ EWAFDPYPLL++WE E ++ Sbjct: 297 TIGWVGSSGYGKPGTQGKFPPHLHYGVYRDRGLIEWAFDPYPLLRRWEQDERNRLK 352 >B3KKR5.1 B3KKR5_9BACI Peptidase M23B Length = 360 Score = 400 bits (1028), Expect = e-109, Method: Compositional matrix adjust. Identities = 187/298 (62%), Positives = 218/298 (73%), Gaps = 1/298 (0%) Query: 56 RKQLYDQMSAATGIAWFRFAAIDQYERTIAKKKSGTAGQAQPRLTGISFPPPVWSGPLNP 115 RK LYDQ+S T I W R AAIDQYERT+ + R GI P W+GPLNP Sbjct: 61 RKSLYDQVSTVTQIPWHRLAAIDQYERTLTRVHPKDRKHPD-RTLGIFIPSTRWAGPLNP 119 Query: 116 NQEDQSPESIRFFXXXXXXXXXXXXADPENDADVLYSMARHLMKYGDSSSDFNIGVWEYY 175 NQED +P SI F ADP ND D+LY++A L YG ++ DF+IG+W YY Sbjct: 120 NQEDVNPTSISVFSGIGLDGDGDGKADPNNDIDLLYTVATRLSPYGHAADDFSIGLWSYY 179 Query: 176 HNGRATQRIEQFARLYEHFGRLDLSGSAFPLPVGKTYSYRSTWGTGRSWGGARIHEGTDL 235 N RA QRI Q+A+LYE FG+LDL AFPLPV YSYRST+G GR+WGG R+HEGTDL Sbjct: 180 QNTRAVQRIMQYAKLYETFGKLDLFDHAFPLPVKSNYSYRSTFGMGRNWGGKRVHEGTDL 239 Query: 236 FTPYGMTVRSTCFGIVETKGWNRYGGWRIGIRDIENRYHYYAHLAGFEKSLSRGDIVAPG 295 F YG+ VRSTC+G+VE KGWN+YGGWRIGIRD+ N YHYYAHL GF+K +S GD+V PG Sbjct: 240 FASYGVPVRSTCYGVVEIKGWNKYGGWRIGIRDLNNHYHYYAHLQGFDKGISLGDVVTPG 299 Query: 296 QTIGWVGSSGYGNPGTQGKFPPHLHYGIYRDRGITEWAFDPYPLLKQWETQEYRAMRK 353 QT+GWVGSSGYG PGTQGKFPPHLHYGIYRD G+TEWAFDPYPLLK WE +E R+ +K Sbjct: 300 QTLGWVGSSGYGKPGTQGKFPPHLHYGIYRDTGLTEWAFDPYPLLKHWELEEKRSKKK 357 >Q65FB3.1 Q65FB3_BACLD YunA Length = 326 Score = 340 bits (871), Expect = 2e-91, Method: Compositional matrix adjust. Identities = 167/308 (54%), Positives = 200/308 (64%), Gaps = 2/308 (0%) Query: 45 AASGKESLSVSRKQLYDQMSAATGIAWFRFAAIDQYERTIAKKKSGTAGQAQPRLTGISF 104 A KE R LY + AAT I W+ FAA+DQYE I K + Q TGI Sbjct: 21 AKEKKEPDLQKRMALYHKTEAATHIPWYVFAAVDQYEINIRKNRKDLPKQKG--HTGIYI 78 Query: 105 PPPVWSGPLNPNQEDQSPESIRFFXXXXXXXXXXXXADPENDADVLYSMARHLMKYGDSS 164 P VWSGP NPN +D SP SI+ F A+P+ D DVLY+ ++L+ YG Sbjct: 79 PQSVWSGPENPNPKDTSPLSIKVFDGIGMDGNGDGLAEPDQDEDVLYTFGQYLLTYGRDI 138 Query: 165 SDFNIGVWEYYHNGRATQRIEQFARLYEHFGRLDLSGSAFPLPVGKTYSYRSTWGTGRSW 224 + IG+WE+Y + I F RL++ +G +DL AFPLPV YSYRSTWG R + Sbjct: 139 ENIRIGLWEFYERDQTVGIISGFIRLFQKYGHIDLGKHAFPLPVRSDYSYRSTWGDARGF 198 Query: 225 GGARIHEGTDLFTPYGMTVRSTCFGIVETKGWNRYGGWRIGIRDIENRYHYYAHLAGFEK 284 GG RIHEGTDLF YG+ VRSTC+GIVE KGWNR+GGWRIGIRDI N YHY+AHL GF K Sbjct: 199 GGRRIHEGTDLFAHYGLPVRSTCYGIVEMKGWNRFGGWRIGIRDINNTYHYFAHLNGFTK 258 Query: 285 SLSRGDIVAPGQTIGWVGSSGYGNPGTQGKFPPHLHYGIYRDRGITEWAFDPYPLLKQWE 344 L G+IV PGQ IG VG++GYG PGT GKFPPHLHYG+Y+D G TEW+FDPYP LK WE Sbjct: 259 GLKVGEIVEPGQVIGSVGATGYGPPGTSGKFPPHLHYGMYKDNGRTEWSFDPYPHLKAWE 318 Query: 345 TQEYRAMR 352 +E R + Sbjct: 319 RKELRKKK 326 >A7Z8E9.1 A7Z8E9_BACA2 YunA Length = 325 Score = 332 bits (850), Expect = 4e-89, Method: Compositional matrix adjust. Identities = 163/312 (52%), Positives = 201/312 (64%), Gaps = 5/312 (1%) Query: 41 VSSEAASGKES---LSVSRKQLYDQMSAATGIAWFRFAAIDQYERTIAKKKSGTAGQAQP 97 V AASGKE + R QLY + A T I W+ AA+DQYE + + +A Sbjct: 13 VFCPAASGKEQSAPVYGERMQLYRKAEAVTQIPWYVLAAVDQYEENVRSNRKDLPEKAG- 71 Query: 98 RLTGISFPPPVWSGPLNPNQEDQSPESIRFFXXXXXXXXXXXXADPENDADVLYSMARHL 157 + I P +WSGP NPN +D +P SI+ F A+ ND D+LY+ A++L Sbjct: 72 -IISIYIPDHLWSGPENPNPKDDAPLSIKVFDGIGLDGDGDGKAEVSNDEDILYTFAQYL 130 Query: 158 MKYGDSSSDFNIGVWEYYHNGRATQRIEQFARLYEHFGRLDLSGSAFPLPVGKTYSYRST 217 +G IG+W YY + I +F +L++ FGRLDL AFPLPV YSYRST Sbjct: 131 AGFGTDLDHIRIGLWNYYRRDQTVGIITEFMKLFKTFGRLDLGEHAFPLPVKTDYSYRST 190 Query: 218 WGTGRSWGGARIHEGTDLFTPYGMTVRSTCFGIVETKGWNRYGGWRIGIRDIENRYHYYA 277 WG R +GG RIHEGTDLF YG+ VRSTC+GIVE KGWNR+GGWRIGIRDI N YHY+A Sbjct: 191 WGAARGFGGRRIHEGTDLFAHYGLPVRSTCYGIVEMKGWNRFGGWRIGIRDINNTYHYFA 250 Query: 278 HLAGFEKSLSRGDIVAPGQTIGWVGSSGYGNPGTQGKFPPHLHYGIYRDRGITEWAFDPY 337 HL GF K + +G IV PG+ IG VGSSGYG PGT GKFPPHLHYG+Y+D G +EW+FDPY Sbjct: 251 HLNGFAKGVHKGQIVKPGEVIGSVGSSGYGPPGTAGKFPPHLHYGMYKDNGRSEWSFDPY 310 Query: 338 PLLKQWETQEYR 349 P L+ WE +E R Sbjct: 311 PHLRAWERKEIR 322 >A8FH35.1 A8FH35_BACP2 M23B subfamily peptidase Length = 326 Score = 332 bits (850), Expect = 5e-89, Method: Compositional matrix adjust. Identities = 156/307 (50%), Positives = 200/307 (65%), Gaps = 2/307 (0%) Query: 41 VSSEAASGKESLSVSRKQLYDQMSAATGIAWFRFAAIDQYERTIAKKKSGTAGQAQPRLT 100 V ++ ++ + R LY++++ T + W+ AA+DQYE I K + Q + Sbjct: 18 VHAQGEGKQQDVLKQRMALYEKVAITTQVPWYVLAAVDQYEHNIRKSRRDLP--KQKGVI 75 Query: 101 GISFPPPVWSGPLNPNQEDQSPESIRFFXXXXXXXXXXXXADPENDADVLYSMARHLMKY 160 GI P +W GP NPN++D SP SI+ F A ++D DVL++ A++L+ Y Sbjct: 76 GIYIPREMWIGPENPNKQDTSPLSIKVFDGIGLDGNGDGKASSDDDEDVLFTFAQYLLHY 135 Query: 161 GDSSSDFNIGVWEYYHNGRATQRIEQFARLYEHFGRLDLSGSAFPLPVGKTYSYRSTWGT 220 G+S I +W+YY + I F +LY+H+G LDL AFPLPVG YSYRSTWG Sbjct: 136 GNSIDQLKIALWDYYGRDQTVGIISSFMKLYQHYGHLDLGKHAFPLPVGADYSYRSTWGD 195 Query: 221 GRSWGGARIHEGTDLFTPYGMTVRSTCFGIVETKGWNRYGGWRIGIRDIENRYHYYAHLA 280 R +GG RIHEGTDLF YG+ VR+T +G++E KGWNR+GGWRIGIRDI N YHY+AHL Sbjct: 196 ARGFGGRRIHEGTDLFAHYGLPVRATSYGVIEMKGWNRFGGWRIGIRDIHNHYHYFAHLN 255 Query: 281 GFEKSLSRGDIVAPGQTIGWVGSSGYGNPGTQGKFPPHLHYGIYRDRGITEWAFDPYPLL 340 GF K L G IV PGQ IG VGSSGYG PGT GKFPPHLHYG+Y+D G TEW+FDPYP L Sbjct: 256 GFAKGLKTGQIVEPGQVIGSVGSSGYGPPGTAGKFPPHLHYGMYKDNGRTEWSFDPYPHL 315 Query: 341 KQWETQE 347 + WE E Sbjct: 316 RAWERAE 322 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.135 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,317,540,823 Number of extensions: 101045007 Number of successful extensions: 213846 Number of sequences better than 1.0e-05: 410 Number of HSP's gapped: 214167 Number of HSP's successfully gapped: 410 Length of query: 355 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 218 Effective length of database: 1,770,017,148 Effective search space: 385863738264 Effective search space used: 385863738264 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)