BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0420.0030.p (365 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KKR4.1 B3KKR4_9BACI Lipoic acid synthetase 536 e-150 B1DFD1.1 B1DFD1_9BACL Lipoic acid synthetase 488 e-136 C0ZKV1.1 C0ZKV1_BREBN Lipoyl synthase EC=2.8.1.8 451 e-125 Q9K7C9.1 LIPA_BACHD Lipoyl synthase EC=2.8.1.8 420 e-116 B7DQZ4.1 B7DQZ4_9BACL Lipoic acid synthetase 419 e-115 >B3KKR4.1 B3KKR4_9BACI Lipoic acid synthetase Length = 297 Score = 536 bits (1382), Expect = e-150, Method: Compositional matrix adjust. Identities = 250/293 (85%), Positives = 273/293 (93%) Query: 72 KQPKPDWIKIKLTTGDNYQEIKGMMRSKTLHTVCEEARCPNIYECWANRTATFMILGDIC 131 KQPKPDWI+IKLTTGDNY+EIK MMRSKTLHTVCEEARCPNIYECWANRTATFMILGDIC Sbjct: 5 KQPKPDWIRIKLTTGDNYKEIKSMMRSKTLHTVCEEARCPNIYECWANRTATFMILGDIC 64 Query: 132 TRACRFCAVNTGLPTELDLQEPERVAEAAENMKLKHCVVTSVARDDLKDGGARIFAETVA 191 TRACRFCAVNTGLPTELDLQEPERVAEAAE M L+HCVVTSVARDDLKDGGA IFAETV Sbjct: 65 TRACRFCAVNTGLPTELDLQEPERVAEAAEQMNLRHCVVTSVARDDLKDGGAYIFAETVK 124 Query: 192 AIRKRLPLCSVEVLIPDFLGERDSLEIVMNSRPDILNHNIETVERMSDRVRARAKYRRSL 251 AIR+RLPLCSVEVLIPDF+G+ +SL+IVM+ +PDILNHNIETVERMSDRVRA+AKY RSL Sbjct: 125 AIRRRLPLCSVEVLIPDFMGDEESLKIVMDVKPDILNHNIETVERMSDRVRAKAKYSRSL 184 Query: 252 ELLSRAKEIKPDIPTKSSIMLGVGEEWDEILQAMDDLRAVDCDILTLGQYLQPSPKHLNV 311 +LL AK ++PDIPTKSSIMLGVGE+W EIL+AMDDLR+V CDI+T+GQYLQPSPKHLNV Sbjct: 185 QLLKNAKAMQPDIPTKSSIMLGVGEQWHEILEAMDDLRSVGCDIMTIGQYLQPSPKHLNV 244 Query: 312 EKFYNPEEFALLKEEGLKRGFSHVESGPLVRSSYHAHEQVKSAAAAREERTVS 364 EK+Y PEEFA+LKEEG+KRGFSHVESGPLVRSSYHAHEQVKSA +EE S Sbjct: 245 EKYYTPEEFAILKEEGMKRGFSHVESGPLVRSSYHAHEQVKSAERNKEETVQS 297 >B1DFD1.1 B1DFD1_9BACL Lipoic acid synthetase Length = 300 Score = 488 bits (1256), Expect = e-136, Method: Compositional matrix adjust. Identities = 231/293 (78%), Positives = 259/293 (88%), Gaps = 1/293 (0%) Query: 72 KQPKPDWIKIKLTTGDNYQEIKGMMRSKTLHTVCEEARCPNIYECWANRTATFMILGDIC 131 K KPDW+KIKLTT NY EIK MMR+KTLHTVCEEARCPNIYECWANRTATFMILGDIC Sbjct: 7 KLKKPDWLKIKLTTEGNYTEIKDMMRTKTLHTVCEEARCPNIYECWANRTATFMILGDIC 66 Query: 132 TRACRFCAVNTGLPTELDLQEPERVAEAAENMKLKHCVVTSVARDDLKDGGARIFAETVA 191 TRACRFCAV TGLPTELDLQEP+RVAEAAE M L+HCVVTSVARDDL DGGA IFA T+ Sbjct: 67 TRACRFCAVKTGLPTELDLQEPQRVAEAAEQMGLRHCVVTSVARDDLADGGASIFAGTIK 126 Query: 192 AIRKRLPLCSVEVLIPDFLGERDSLEIVMNSRPDILNHNIETVERMSDRVRARAKYRRSL 251 AIR+R+P VEVLIPDF+G +SL+IVM+++PDILNHNIETVERMS+RVRA+AKYRRSL Sbjct: 127 AIRERMPFTRVEVLIPDFMGNEESLKIVMDAKPDILNHNIETVERMSNRVRAKAKYRRSL 186 Query: 252 ELLSRAKEIKPDIPTKSSIMLGVGEEWDEILQAMDDLRAVDCDILTLGQYLQPSPKHLNV 311 ELL RAKE+ P IPTKSSIMLGVGE ++E+LQAMDDLRAVDC+ILTLGQYLQP+P H+ + Sbjct: 187 ELLRRAKEMNPKIPTKSSIMLGVGETYEEVLQAMDDLRAVDCNILTLGQYLQPTPNHMPI 246 Query: 312 EKFYNPEEFALLKEEGLKRGFSHVESGPLVRSSYHAHEQVKSAAAAREERTVS 364 E++ +P+EF LKEEG KRGF HVESGPLVRSSYHAHEQ SAA A EER +S Sbjct: 247 ERYVHPDEFKSLKEEGYKRGFRHVESGPLVRSSYHAHEQTDSAAEA-EERELS 298 >C0ZKV1.1 C0ZKV1_BREBN Lipoyl synthase EC=2.8.1.8 Length = 300 Score = 451 bits (1160), Expect = e-125, Method: Compositional matrix adjust. Identities = 209/290 (72%), Positives = 252/290 (86%), Gaps = 6/290 (2%) Query: 73 QPKPDWIKIKLTTGD---NYQEIKGMMRSKTLHTVCEEARCPNIYECWANRTATFMILGD 129 Q KP+W+KI L +G +++E+K MR+KTLHTVCEEA+CPNI+ECWA+ TATFMILGD Sbjct: 3 QRKPEWLKINLVSGSELASFKELKQTMRTKTLHTVCEEAKCPNIHECWASGTATFMILGD 62 Query: 130 ICTRACRFCAVNTGLPTELDLQEPERVAEAAENMKLKHCVVTSVARDDLKDGGARIFAET 189 ICTRACRFCAV TGLPTELD EPERVAEAAE M+LKH VVTSVARDDL DGGA+IFA+T Sbjct: 63 ICTRACRFCAVKTGLPTELDTAEPERVAEAAEQMRLKHVVVTSVARDDLADGGAQIFADT 122 Query: 190 VAAIRKRLPLCSVEVLIPDFLGERDSLEIVMNSRPDILNHNIETVERMSDRVRARAKYRR 249 + AIR+RLP SVEVLIPDF+G D+L++VM+++PD+LNHNIE V RMSDRVRARAKY R Sbjct: 123 IRAIRRRLPFASVEVLIPDFMGNWDALKVVMDAKPDVLNHNIEAVRRMSDRVRARAKYDR 182 Query: 250 SLELLSRAKEIKPDIPTKSSIMLGVGEEWDEILQAMDDLRAVDCDILTLGQYLQPSPKHL 309 +LELL +AKE +P IPTKSS+M+GVGE +EI++ MDDLR+VD +I+T+GQYLQP+ KHL Sbjct: 183 TLELLKKAKEFQPSIPTKSSLMIGVGETMEEIIETMDDLRSVDVNIMTIGQYLQPTKKHL 242 Query: 310 NVEKFYNPEEFALLKEEGLKRGFSHVESGPLVRSSYHAHEQVKSAAAARE 359 VEKFY+P+EFA LK+EG+ RGFSHVESGPLVRSSYHAHEQ A+AA+E Sbjct: 243 KVEKFYHPDEFARLKDEGMNRGFSHVESGPLVRSSYHAHEQ---ASAAKE 289 >Q9K7C9.1 LIPA_BACHD Lipoyl synthase EC=2.8.1.8 Length = 303 Score = 420 bits (1080), Expect = e-116, Method: Compositional matrix adjust. Identities = 196/291 (67%), Positives = 237/291 (81%), Gaps = 1/291 (0%) Query: 75 KPDWIKIKLTTGDNYQEIKGMMRSKTLHTVCEEARCPNIYECWANR-TATFMILGDICTR 133 KPDW+KIKL T + Y +K MMR K LHTVCEEA+CPNI+ECWA R TATFMILGD+CTR Sbjct: 10 KPDWLKIKLNTNETYTGLKKMMREKNLHTVCEEAKCPNIHECWAVRKTATFMILGDVCTR 69 Query: 134 ACRFCAVNTGLPTELDLQEPERVAEAAENMKLKHCVVTSVARDDLKDGGARIFAETVAAI 193 ACRFCAV TGLP ELDLQEPERVAE+ E M LKH V+T+VARDDLKDGGA +FAETV A+ Sbjct: 70 ACRFCAVKTGLPNELDLQEPERVAESVEIMGLKHAVITAVARDDLKDGGAGVFAETVRAV 129 Query: 194 RKRLPLCSVEVLIPDFLGERDSLEIVMNSRPDILNHNIETVERMSDRVRARAKYRRSLEL 253 R+R P C++EVL D +G D+L+ +M++RP+ILNHNIETV R++ RVRARA Y RSLE Sbjct: 130 RRRNPFCTIEVLPSDMMGNDDNLKTLMDARPNILNHNIETVRRLTPRVRARATYERSLEF 189 Query: 254 LSRAKEIKPDIPTKSSIMLGVGEEWDEILQAMDDLRAVDCDILTLGQYLQPSPKHLNVEK 313 L RAKE++PDIPTKSS+M+G+GE DEI++ MDDLRA + DI+T+GQYLQP+ KHL V+K Sbjct: 190 LRRAKEMQPDIPTKSSLMIGLGETKDEIIETMDDLRANNVDIMTIGQYLQPTKKHLKVQK 249 Query: 314 FYNPEEFALLKEEGLKRGFSHVESGPLVRSSYHAHEQVKSAAAAREERTVS 364 +Y+P+EFA LKE L +GFSH E+GPLVRSSYHA EQV A E R + Sbjct: 250 YYHPDEFAELKEIALSKGFSHCEAGPLVRSSYHADEQVNEAQVREEARKAA 300 >B7DQZ4.1 B7DQZ4_9BACL Lipoic acid synthetase Length = 308 Score = 419 bits (1077), Expect = e-115, Method: Compositional matrix adjust. Identities = 190/279 (68%), Positives = 237/279 (84%) Query: 73 QPKPDWIKIKLTTGDNYQEIKGMMRSKTLHTVCEEARCPNIYECWANRTATFMILGDICT 132 + +P+W+KI+ TG+NY+++K +MR+++LHTVCEEA CPN++ECW RTATFMILGDICT Sbjct: 17 KARPEWLKIRAKTGENYKQLKEIMRTQSLHTVCEEAHCPNLFECWEMRTATFMILGDICT 76 Query: 133 RACRFCAVNTGLPTELDLQEPERVAEAAENMKLKHCVVTSVARDDLKDGGARIFAETVAA 192 RACRFCAV+TG P ELDL+EPERVA+A M L+H V+TSVARDDL DGGA +FAET+ A Sbjct: 77 RACRFCAVHTGRPNELDLREPERVADAVVAMGLEHVVITSVARDDLADGGASVFAETIRA 136 Query: 193 IRKRLPLCSVEVLIPDFLGERDSLEIVMNSRPDILNHNIETVERMSDRVRARAKYRRSLE 252 +R+++P CSVEVLIPDF G D+L V+++RPD+LNHNIETV R+SD VR+RAKY R+LE Sbjct: 137 VRRKVPTCSVEVLIPDFDGNWDALAAVLDARPDVLNHNIETVRRLSDSVRSRAKYDRTLE 196 Query: 253 LLSRAKEIKPDIPTKSSIMLGVGEEWDEILQAMDDLRAVDCDILTLGQYLQPSPKHLNVE 312 LL R+KEI PDIPTKSSIM+G+GE ++EI + MDDLRAVD DILT+GQYLQP+ KHL V Sbjct: 197 LLRRSKEIAPDIPTKSSIMVGLGETFEEIYETMDDLRAVDVDILTIGQYLQPTAKHLPVV 256 Query: 313 KFYNPEEFALLKEEGLKRGFSHVESGPLVRSSYHAHEQV 351 ++Y P EF L+ EGLKRGF HVESGP+VRSSYHA +QV Sbjct: 257 RYYTPTEFLRLRGEGLKRGFKHVESGPMVRSSYHAKDQV 295 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,049,943,945 Number of extensions: 79820150 Number of successful extensions: 232610 Number of sequences better than 1.0e-05: 1401 Number of HSP's gapped: 230538 Number of HSP's successfully gapped: 1402 Length of query: 365 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 228 Effective length of database: 1,770,017,148 Effective search space: 403563909744 Effective search space used: 403563909744 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)