BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0420.0022.p (479 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q8CUR8.1 Q8CUR8_OCEIH Multidrug resistance protein (Efflux trans... 327 2e-87 A8VQE6.1 A8VQE6_9BACI Diguanylate cyclase 327 2e-87 Q65F10.1 Q65F10_BACLD Putative uncharacterized protein 325 8e-87 Q8EU23.1 Q8EU23_OCEIH Multidrug resistance protein 310 2e-82 Q65NE1.1 Q65NE1_BACLD YcnB 308 6e-82 >Q8CUR8.1 Q8CUR8_OCEIH Multidrug resistance protein (Efflux transporter) Length = 470 Score = 327 bits (838), Expect = 2e-87, Method: Compositional matrix adjust. Identities = 175/454 (38%), Positives = 277/454 (61%), Gaps = 5/454 (1%) Query: 8 HAMKRKPVIIVASLIVGNFLAQLMQTMLNTALPQMMSDLGIQANRAQWLITVYLLVSGIV 67 M R P+++V L+ G F+A L QT+L TALP +MSDL ++AN AQWL + ++LV+GI+ Sbjct: 11 EKMSRVPILVV--LLSGAFVAILNQTLLGTALPHIMSDLELEANTAQWLQSAFMLVNGIM 68 Query: 68 IPVTGFLIGKYSTRALFFASVGAFTTGTLIAGFSSEFAFILIGRIIQGVGAGLLIPVFLN 127 IP+T FLIG ++TR+LF A++ +F GTLI G + F +L GRI+Q GAG+++P+ Sbjct: 69 IPITAFLIGTFTTRSLFLAAMSSFAIGTLICGIAPNFELLLTGRILQAAGAGIIMPLMQT 128 Query: 128 TIIMVFPKEKIGAAMGLASLIVGLAPAMGPTISGFVVQDHSWRILFFGVAPIAIANFFVA 187 +++++P+E+ G+AMG+ LI+ APA+GP++SG++V+ WR LFF V PIAI N +A Sbjct: 129 IMMLIYPREQRGSAMGMFGLIISFAPAIGPSLSGYIVEHSPWRTLFFMVLPIAIINIGIA 188 Query: 188 YYCLENVGETNKTKLDRRSVLYSTLGFGSLLYGFSILGEQGRXXXXXXXXXXXGFIITFL 247 Y+ L+NV + +LD+ S++ STLGFG LLYGFSI G G G I Sbjct: 189 YFLLKNVTKRTFPQLDKLSIILSTLGFGGLLYGFSIAGSAGWLSNQVIISMIVGIIALAW 248 Query: 248 FVKRQFKLAVPLLDFNVFRYPRFTYASIIGVILFIVMAGVELLLPIYAQNVRGLMPRESX 307 F+ RQ L P+L+F VF+Y FT +I+G++ F+ M G ++LP+Y Q++ G ES Sbjct: 249 FILRQLNLKTPILEFRVFQYKIFTITTILGMVTFMTMIGAAVILPLYMQDMLGFSAFESG 308 Query: 308 XXXXXXXXXXXVSGVISGKIYDRFGGRHLIRTGFFWIAAVSSLLTMTLTVHASFSLLMGI 367 + ++G+++DR+G R+L G I + + M LT SF+ + I Sbjct: 309 LALLAGAVLQGIMNPVTGRLFDRYGSRYLAIIGLGLIVVTTFMFGM-LTAETSFTYIATI 367 Query: 368 YALLMTGIGFMMTPITALAMASLPNSIMKYASPMTTAIRTLSMSMGGALLITLMTITADY 427 +A+ M G+ M P+T + SLP+ + + + + +R +S ++G AL IT+M+ TA Sbjct: 368 HAIRMLGVAMAMMPVTTAGLNSLPDEFIAHGTAVNNTLRQVSGAIGTALPITIMS-TAAI 426 Query: 428 SSVSFPVNMLEGIRAAFWTLTVIAAGGFFLSFFV 461 S ++ G+ +F +I+ G L+FF+ Sbjct: 427 PSEGLQ-GLIHGVNVSFIVTAIISIVGLILAFFI 459 >A8VQE6.1 A8VQE6_9BACI Diguanylate cyclase Length = 471 Score = 327 bits (838), Expect = 2e-87, Method: Compositional matrix adjust. Identities = 180/463 (38%), Positives = 275/463 (59%), Gaps = 5/463 (1%) Query: 17 IVASLIVGNFLAQLMQTMLNTALPQMMSDLGIQANRAQWLITVYLLVSGIVIPVTGFLIG 76 IVA L+ G F+A L QT+L TA+P +M DLGI AN AQW+ T+++LV+GI+IP+T FLIG Sbjct: 10 IVAVLLTGTFVAILNQTLLVTAIPPIMRDLGINANMAQWVNTIFMLVNGIMIPITAFLIG 69 Query: 77 KYSTRALFFASVGAFTTGTLIAGFSSEFAFILIGRIIQGVGAGLLIPVFLNTIIMVFPKE 136 K++TR LF ++ F GTL+AG S F +++GRIIQ GAG++IP+ + M+FP E Sbjct: 70 KFTTRKLFMTAMSLFALGTLVAGLSPNFETLILGRIIQASGAGIMIPLMQTVLFMIFPVE 129 Query: 137 KIGAAMGLASLIVGLAPAMGPTISGFVVQDHSWRILFFGVAPIAIANFFVAYYCLENVGE 196 K G AMGL L++ APA+GPT+SGF+V + WR+LFF V PIA+ + VAY+ L+NV + Sbjct: 130 KRGQAMGLVGLVIAFAPAIGPTLSGFIVDQYPWRLLFFIVLPIALIDLVVAYFVLKNVTK 189 Query: 197 TNKTKLDRRSVLYSTLGFGSLLYGFSILGEQGRXXXXXXXXXXXGFIITFLFVKRQFKLA 256 +D +S++ ST GFG LL+ FS G G G + F+ RQ ++ Sbjct: 190 QTFPTIDIKSIMLSTFGFGGLLFAFSYAGTTGWTSWEVLVSTGVGGVTLTWFILRQLRIP 249 Query: 257 VPLLDFNVFRYPRFTYASIIGVILFIVMAGVELLLPIYAQNVRGLMPRESXXXXXXXXXX 316 P+L+F VFRY F +++IG+++F+ M G +LPIY Q++ G +S Sbjct: 250 EPILEFRVFRYSIFALSNVIGIVVFMSMIGAATILPIYMQDMLGFSAFQSGLMLLPGALL 309 Query: 317 XXVSGVISGKIYDRFGGRHLIRTGFFWIAAVSSLLTMTLTVHASFSLLMGIYALLMTGIG 376 + I+G+I+D+ G R L +G + ++ L T LT SF + ++A M + Sbjct: 310 MGLLNPITGRIFDKVGARWLSISGMALLTVMTFLFT-RLTEDTSFLYMTTVHAFRMMSLA 368 Query: 377 FMMTPITALAMASLPNSIMKYASPMTTAIRTLSMSMGGALLITLMTITADYSSVSFPVN- 435 +M PIT + LP ++ + + M +R + S+G A L+T+MT+ A +SS +N Sbjct: 369 MVMMPITTAGLNQLPERLIPHGTAMNNTLRQVGGSLGTAFLVTVMTVGAAFSSNGRTMNE 428 Query: 436 -MLEGIRAAFWTLTVIAAGGFFLSFFVPYAQGSAHPQVKESRQ 477 M++G+ +FW T +A GF ++ FV +V+ES Sbjct: 429 AMIQGVNFSFWVATFLAFIGFVIAMFV--KNPPKRQEVEESTN 469 >Q65F10.1 Q65F10_BACLD Putative uncharacterized protein Length = 483 Score = 325 bits (832), Expect = 8e-87, Method: Compositional matrix adjust. Identities = 186/474 (39%), Positives = 280/474 (59%), Gaps = 10/474 (2%) Query: 7 EHAMKRKPVIIVASLIVGNFLAQLMQTMLNTALPQMMSDLGIQANRAQWLITVYLLVSGI 66 +H + P IVA LI+G F+A L QT+L TALP +M DL I AQWL TV++LV+GI Sbjct: 5 QHKTNQLP--IVAVLIMGTFIAILNQTLLTTALPPIMRDLNITPGLAQWLTTVFMLVNGI 62 Query: 67 VIPVTGFLIGKYSTRALFFASVGAFTTGTLIAGFSSEFAFILIGRIIQGVGAGLLIPVFL 126 +IPVT FLI K++TR LF A++ FT GTLI S F ++ GR++Q GAG+++P+ Sbjct: 63 MIPVTAFLIEKFTTRKLFMAAMSLFTAGTLICALSFSFPALIAGRVVQAAGAGIMMPLMQ 122 Query: 127 NTIIMVFPKEKIGAAMGLASLIVGLAPAMGPTISGFVVQDHSWRILFFGVAPIAIANFFV 186 ++++FPKEK G+AMG+ L++ APA+GPT+SG++V + W +LF+ + P A+ + Sbjct: 123 TVLLLIFPKEKRGSAMGMVGLVIAFAPAVGPTLSGWIVDRYPWEVLFYLLLPFAVIDMIA 182 Query: 187 AYYCLENVGETNKTKLDRRSVLYSTLGFGSLLYGFSILGEQGRXXXXXXXXXXXGFIITF 246 A++ L+NV E K+D S++ S+LGFG +LYGFS G G G + F Sbjct: 183 AHFILKNVTEQTSPKMDVTSIILSSLGFGGILYGFSSAGVAGWTGADVLAGFAVGSVALF 242 Query: 247 LFVKRQFKLAVPLLDFNVFRYPRFTYASIIGVILFIVMAGVELLLPIYAQNVRGLMPRES 306 F+ RQ L P+L+F VF+YP FT A+IIG+++F+ M G ++P+Y Q++R ES Sbjct: 243 WFIWRQLHLEEPILEFRVFKYPMFTLATIIGMVVFMSMIGAATIVPMYMQDMRHFTAMES 302 Query: 307 XXXXXXXXXXXXVSGVISGKIYDRFGGRHLIRTGFFWIAAVSSLLTMTLTVHASFSLLMG 366 + I+G+I+D+ G R L TG I V++LL LT F+ L Sbjct: 303 GMMLFPGAVVMGLMSPITGRIFDKIGARILSITGLTAI-FVTTLLLTHLTKTTPFAYLSI 361 Query: 367 IYALLMTGIGFMMTPITALAMASLPNSIMKYASPMTTAIRTLSMSMGGALLITLMTITA- 425 +YA+ M G+G ++ P+T + LP ++ + + M +R +S S+G A+LIT+MT A Sbjct: 362 VYAIRMVGMGMVLMPVTTAGLNQLPRHLIPHGTAMNNTMRQMSGSIGTAVLITIMTQRAL 421 Query: 426 -DYSSVSFPVNMLEGIRAAFWTLTVIAAGGFFLSFFVPYAQ-----GSAHPQVK 473 D + S M+ G+ AF T ++ G LSFF+ + GS Q K Sbjct: 422 LDPAGPSSDTAMIHGVNLAFMTAAALSGIGLVLSFFIKQHRRGRLDGSKRKQAK 475 >Q8EU23.1 Q8EU23_OCEIH Multidrug resistance protein Length = 509 Score = 310 bits (794), Expect = 2e-82, Method: Compositional matrix adjust. Identities = 176/485 (36%), Positives = 280/485 (57%), Gaps = 20/485 (4%) Query: 10 MKRKPVIIVASLIVGNFLAQLMQTMLNTALPQMMSDLGIQANRAQWLITVYLLVSGIVIP 69 + + P+I V +++G+F+A L QT+LN ALP M+ D+ + AN+AQ L T+++LV+GI+IP Sbjct: 13 LPKVPIIFV--MVMGSFIAILNQTLLNVALPVMIVDMNMSANQAQSLTTIFMLVNGILIP 70 Query: 70 VTGFLIGKYSTRALFFASVGAFTTGTLIAGFSSEFAFILIGRIIQGVGAGLLIPVFLNTI 129 +T FL+ K++TR LF ++ F GTL+ G + + +L+GR+IQ GAG+++P+ +N I Sbjct: 71 ITAFLMEKFTTRQLFLTAMSLFALGTLVCGLAFGYPSLLVGRVIQAAGAGIMMPLLMNVI 130 Query: 130 IMVFPKEKIGAAMGLASLIVGLAPAMGPTISGFVVQDHSWRILFFGVAPIAIANFFVAYY 189 + +FP K G+AMG L + APA+GPT+SG VVQ++SWR+LFF V PIAI + +A++ Sbjct: 131 LSLFPINKRGSAMGYIGLAMMFAPAIGPTLSGLVVQNYSWRVLFFIVLPIAIIDIILAFF 190 Query: 190 CLENVGETNKTKLDRRSVLYSTLGFGSLLYGFSILGEQGRXXXXXXXXXXXGFIITFLFV 249 L NV + + K++ S+L ST+ FG LL GFS+ GE G G I+ F+ Sbjct: 191 FLRNVTKQSNPKVNYISILMSTVAFGGLLLGFSLAGEMGWKHVEVIAPLMIGAIVMVFFI 250 Query: 250 KRQFKLAVPLLDFNVFRYPRFTYASIIGVILFIVMAGVELLLPIYAQNVRGLMPRESXXX 309 + Q KL P+L+F VF++ F+ ++I V++ + M +L+PIY QN+RG P S Sbjct: 251 RMQLKLKNPILEFRVFKFSMFSLTTVISVVITMSMFAAMILVPIYLQNLRGFSPLASGLM 310 Query: 310 XXXXXXXXXVSGVISGKIYDRFGGRHLIRTGFFWIAAVSSLLTMTLTVHASFSLLMGIYA 369 I+GK++DR G R L TG I A+++ L L+ + LM +Y+ Sbjct: 311 LLPGALISAFMSPITGKLFDRIGARPLAITG-LGITAITTFLFSDLSNETGYYYLMFVYS 369 Query: 370 LLMTGIGFMMTPITALAMASLPNSIMKYASPMTTAIRTLSMSMGGALLITLMT------- 422 L M GI +M PI + LP + + + M +R +S ++G A L+T+M+ Sbjct: 370 LRMVGISMIMMPIMTAGLNQLPQRLYSHGTAMANTLRQMSGAIGTAFLVTVMSSQTVKHM 429 Query: 423 -------ITADYSSVSFPVNMLEGIRAAFWTLTVIAAGGFFLSFFVPY---AQGSAHPQV 472 ++ + V + +EGI +F T+ A GF LSFF+ + S Sbjct: 430 DEMTQTVVSEEQLKVIENLATIEGINDSFMIATLFAIFGFILSFFIKRRYPPEESVETVS 489 Query: 473 KESRQ 477 KE ++ Sbjct: 490 KEQKR 494 >Q65NE1.1 Q65NE1_BACLD YcnB Length = 473 Score = 308 bits (790), Expect = 6e-82, Method: Compositional matrix adjust. Identities = 172/459 (37%), Positives = 272/459 (59%), Gaps = 5/459 (1%) Query: 16 IIVASLIVGNFLAQLMQTMLNTALPQMMSDLGIQANRAQWLITVYLLVSGIVIPVTGFLI 75 IIV LI G F+A L QT+L TALP +M+DL I A +AQWL T ++L +GI+IP+T FLI Sbjct: 14 IIVGLLIAGAFIAILNQTLLVTALPHIMADLNIDATKAQWLTTAFMLTNGILIPITAFLI 73 Query: 76 GKYSTRALFFASVGAFTTGTLIAGFSSEFAFILIGRIIQGVGAGLLIPVFLNTIIMVFPK 135 K+S+RAL A++ F GT++ + F +L RIIQ GAG+++P+ + +FP Sbjct: 74 EKFSSRALVIAAMSIFAAGTVVGAVAPNFPVLLAARIIQAAGAGIMMPLMQTIFLTIFPV 133 Query: 136 EKIGAAMGLASLIVGLAPAMGPTISGFVVQDHSWRILFFGVAPIAIANFFVAYYCLENVG 195 EK GAAMG+ L++ APA+GPT+SG++V +WR LF+ + PIA+ + + ++NV Sbjct: 134 EKRGAAMGMVGLVIAFAPAIGPTLSGWIVDSFTWRYLFYIILPIALIDLVLVILLMKNVT 193 Query: 196 ETNKTKLDRRSVLYSTLGFGSLLYGFSILGEQGRXXXXXXXXXXXGFIITFLFVKRQFKL 255 + +T +D S++ S+LGFG LLYGFS G +G G + F F+ RQ K+ Sbjct: 194 KLRETSVDIISIILSSLGFGGLLYGFSNAGSEGWGSNTVLISFAVGAVALFFFISRQLKI 253 Query: 256 AVPLLDFNVFRYPRFTYASIIGVILFIVMAGVELLLPIYAQNVRGLMPRESXXXXXXXXX 315 P+L+F VF+ FT +++G+I+F +M G E +LP+Y QNVR + +S Sbjct: 254 KRPILEFRVFQSWTFTITTLLGMIVFALMIGTETILPLYTQNVRDVTAFDSGLMLLPGAI 313 Query: 316 XXXVSGVISGKIYDRFGGRHLIRTGFFWIAAVSSLLTMTLTVHASFSLLMGIYALLMTGI 375 + I G+I+D+ GG+ L GF + ++S+ L + S + ++ IY L M G Sbjct: 314 AMGIMSPIIGRIFDKIGGKGLAIIGFA-VLLLTSIPFADLQMDTSLTFIIVIYTLRMMGT 372 Query: 376 GFMMTPITALAMASLPNSIMKYASPMTTAIRTLSMSMGGALLITLMTITADYSSVSFPVN 435 +M P+T + +LP ++ + + M +R + S+G A+LI++M+ +A ++ S P+N Sbjct: 373 SMIMMPLTTAGINALPAHLIPHGTAMNNTMRQIGGSIGTAVLISVMSNSAKNAATSHPLN 432 Query: 436 -MLEGIRAAFWTLTVIAAGGFFLSFFV---PYAQGSAHP 470 ++ G+ +AF V+A GF LSF + P A P Sbjct: 433 ALIHGMNSAFIVAAVLALIGFILSFTLKKKPQADKQGQP 471 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.329 0.142 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,446,977,844 Number of extensions: 95756036 Number of successful extensions: 462836 Number of sequences better than 1.0e-05: 9862 Number of HSP's gapped: 450068 Number of HSP's successfully gapped: 9923 Length of query: 479 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 339 Effective length of database: 1,741,754,103 Effective search space: 590454640917 Effective search space used: 590454640917 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 133 (55.8 bits)