BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0420.0018.p (267 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KKR2.1 B3KKR2_9BACI NAD(+) kinase EC=2.7.1.23 468 e-130 B1DFD3.1 B1DFD3_9BACL NAD(+) kinase EC=2.7.1.23 367 e-100 B1YJB2.1 B1YJB2_EXIS2 NAD(+) kinase EC=2.7.1.23 290 1e-76 O31612.1 PPNK1_BACSU Probable inorganic polyphosphate/ATP-NAD ki... 285 4e-75 B7GLK4.1 B7GLK4_ANOFW NAD kinase 284 7e-75 >B3KKR2.1 B3KKR2_9BACI NAD(+) kinase EC=2.7.1.23 Length = 267 Score = 468 bits (1203), Expect = e-130, Method: Compositional matrix adjust. Identities = 216/266 (81%), Positives = 247/266 (92%) Query: 1 LRYYVLDRGDELSIKLAEQFHKLAAERNLELDAKSPEIVISIGGDGTMLHAFHTFIDQIP 60 +RYYVLDRGD LS++L +QFHKLA +R LDA+SPEIV+SIGGDGTMLHAFHTFID+IP Sbjct: 1 MRYYVLDRGDPLSVELTQQFHKLAEQRGFVLDAESPEIVVSIGGDGTMLHAFHTFIDRIP 60 Query: 61 NLAFVGVHTGHLGFYADWKADELPALADYMCGTGSAEPHKPRIVKYPLLELEIHKKSGSS 120 +LAFVGVHTGHLGFYADWKADEL L D+M G G KPR+VKYPL++LEIHKKSG+S Sbjct: 61 DLAFVGVHTGHLGFYADWKADELTELIDHMSGEGEHGNVKPRLVKYPLVQLEIHKKSGTS 120 Query: 121 SHIALNEFTLKGVDGTVVIQVDINDVTFEMFRGDGLCVSTPSGSTAYNKSLGGAMVHPTI 180 S+IALNEFTLKGVDGTVV Q+DINDVTFEMFRGDG+CVSTPSGSTAYNKS+GGAMVHP+I Sbjct: 121 SYIALNEFTLKGVDGTVVAQIDINDVTFEMFRGDGICVSTPSGSTAYNKSVGGAMVHPSI 180 Query: 181 EALQIAEIASINNRVFRTMGSPLLLPKHHHCDIFSRKDQRLLLTIDHNNIPVDDLISVRC 240 +ALQIAEIASINNR+FRT+GSPLLLPKHHHCDIFSRK+QRLLLTIDH NI +DDL+SVRC Sbjct: 181 DALQIAEIASINNRIFRTLGSPLLLPKHHHCDIFSRKEQRLLLTIDHVNISIDDLVSVRC 240 Query: 241 QVSDKKISFARYRPFPFWNRVREAFL 266 QV+++++SFARYRPFPFWNRVR+AFL Sbjct: 241 QVAEQQVSFARYRPFPFWNRVRDAFL 266 >B1DFD3.1 B1DFD3_9BACL NAD(+) kinase EC=2.7.1.23 Length = 262 Score = 367 bits (942), Expect = e-100, Method: Compositional matrix adjust. Identities = 164/266 (61%), Positives = 216/266 (81%), Gaps = 6/266 (2%) Query: 1 LRYYVLDRGDELSIKLAEQFHKLAAERNLELDAKSPEIVISIGGDGTMLHAFHTFIDQIP 60 ++Y V+DRGD+LS LAE+FH+LAAER L+ ++P+I++SIGGDGT+L AFH + +++ Sbjct: 1 MKYAVIDRGDQLSKSLAERFHQLAAERGLKRHDETPDIIVSIGGDGTLLQAFHKYTERVT 60 Query: 61 NLAFVGVHTGHLGFYADWKADELPALADYMCGTGSAEPHKPRIVKYPLLELEIHKKSGSS 120 +++FVG+HTGHLGFYADWKADEL L M P +V+YPL E+ + + Sbjct: 61 DVSFVGIHTGHLGFYADWKADELETLVTLMA------EETPHLVRYPLAEIAVETDEQNY 114 Query: 121 SHIALNEFTLKGVDGTVVIQVDINDVTFEMFRGDGLCVSTPSGSTAYNKSLGGAMVHPTI 180 ++ALNEFTLKGVD T+V+Q+ +ND +FEMFRGDG+ +STPSGSTAYNKS+GGA+VHP+I Sbjct: 115 YYLALNEFTLKGVDATLVVQLSVNDESFEMFRGDGIVISTPSGSTAYNKSVGGAIVHPSI 174 Query: 181 EALQIAEIASINNRVFRTMGSPLLLPKHHHCDIFSRKDQRLLLTIDHNNIPVDDLISVRC 240 E+LQIAEIASINNRV+RT+GS LLP+HHHCDI S+K+QRLLLTIDH ++ D+ S+RC Sbjct: 175 ESLQIAEIASINNRVYRTLGSSFLLPQHHHCDIISKKEQRLLLTIDHLSLQRTDIRSIRC 234 Query: 241 QVSDKKISFARYRPFPFWNRVREAFL 266 VS +K+SFARYRPFPFWNRVR+AF+ Sbjct: 235 SVSTRKVSFARYRPFPFWNRVRDAFV 260 >B1YJB2.1 B1YJB2_EXIS2 NAD(+) kinase EC=2.7.1.23 Length = 267 Score = 290 bits (741), Expect = 1e-76, Method: Compositional matrix adjust. Identities = 145/268 (54%), Positives = 186/268 (69%), Gaps = 8/268 (2%) Query: 1 LRYYVLDRGDELSIKLAEQFHKLAAERNLELDAKSPEIVISIGGDGTMLHAFHTFIDQIP 60 +R+ V RGDE S L EQ + ER D +PEIVISIGGDGTML AFH+++DQ+ Sbjct: 1 MRFAVTARGDERSHMLKEQLEQALIERGSHRDVVTPEIVISIGGDGTMLQAFHSYLDQVE 60 Query: 61 NLAFVGVHTGHLGFYADWKADELPALADYMCGTGSAEPHKPRIVKYPLLELEIHKKSGSS 120 + VG+HTGHLGFYADW+ +E+ L ++ A V+YPLLEL I GS+ Sbjct: 61 EITLVGIHTGHLGFYADWRPEEMDELIQHIADDNIA------TVEYPLLELSIDYADGST 114 Query: 121 SHI-ALNEFTLKGVDGTVVIQVDINDVTFEMFRGDGLCVSTPSGSTAYNKSLGGAMVHPT 179 + + ALNE T+K + T+V + I FE FRGDGLC+STPSGSTAYNK+LGGA+VHP Sbjct: 115 NKLLALNECTIKSFNQTLVCDLSIRGDYFETFRGDGLCISTPSGSTAYNKALGGAIVHPA 174 Query: 180 IEALQIAEIASINNRVFRTMGSPLLLPKHHHCDIFSRKDQRLLLTIDH-NNIPVDDLISV 238 +EA+QI E+ASINNRV+RT+GSP+LLPKHH +I LT DH +I ++ S+ Sbjct: 175 LEAIQITEMASINNRVYRTIGSPMLLPKHHDVEIRPVNPIDFQLTYDHYASIVHQNVKSI 234 Query: 239 RCQVSDKKISFARYRPFPFWNRVREAFL 266 RC+VSDKK+ FAR+R FPFW RVRE+FL Sbjct: 235 RCRVSDKKVKFARFRSFPFWQRVRESFL 262 >O31612.1 PPNK1_BACSU Probable inorganic polyphosphate/ATP-NAD kinase 1 EC=2.7.1.23 Length = 266 Score = 285 bits (728), Expect = 4e-75, Method: Compositional matrix adjust. Identities = 137/268 (51%), Positives = 190/268 (70%), Gaps = 8/268 (2%) Query: 1 LRYYVLDRGDELSIKLAEQFHKLAAERNLELDAKSPEIVISIGGDGTMLHAFHTFIDQIP 60 +++ V +GD++S L + + ++ELD PEIVIS+GGDGT+L+AFH + D++ Sbjct: 1 MKFAVSSKGDQVSDTLKSKIQAYLLDFDMELDENEPEIVISVGGDGTLLYAFHRYSDRLD 60 Query: 61 NLAFVGVHTGHLGFYADWKADELPALADYMCGTGSAEPHKPRIVKYPLLELEI--HKKSG 118 AFVGVHTGHLGFYADW E+ L + T P+ V+YPLLE+ + H+ Sbjct: 61 KTAFVGVHTGHLGFYADWVPHEIEKLVLAIAKT----PY--HTVEYPLLEVIVTYHENER 114 Query: 119 SSSHIALNEFTLKGVDGTVVIQVDINDVTFEMFRGDGLCVSTPSGSTAYNKSLGGAMVHP 178 ++ALNE T+K ++G++V V+I FE FRGDGLC+STPSGSTAYNK+LGGA++HP Sbjct: 115 EERYLALNECTIKSIEGSLVADVEIKGQLFETFRGDGLCLSTPSGSTAYNKALGGAIIHP 174 Query: 179 TIEALQIAEIASINNRVFRTMGSPLLLPKHHHCDIFSRKDQRLLLTIDHNNIPVDDLISV 238 +I A+Q+AE+ASINNRVFRT+GSPLLLP HH C I R + +TIDH + D+ S+ Sbjct: 175 SIRAIQLAEMASINNRVFRTVGSPLLLPSHHDCMIKPRNEVDFQVTIDHLTLLHKDVKSI 234 Query: 239 RCQVSDKKISFARYRPFPFWNRVREAFL 266 RCQV+ +K+ FAR+RPFPFW RV+++F+ Sbjct: 235 RCQVASEKVRFARFRPFPFWKRVQDSFI 262 >B7GLK4.1 B7GLK4_ANOFW NAD kinase Length = 263 Score = 284 bits (726), Expect = 7e-75, Method: Compositional matrix adjust. Identities = 136/268 (50%), Positives = 191/268 (71%), Gaps = 8/268 (2%) Query: 1 LRYYVLDRGDELSIKLAEQFHKLAAERNLELDAKSPEIVISIGGDGTMLHAFHTFIDQIP 60 +++ V+ +GD +S + + + L D P+IVIS+GGDGT+L+AFH + ++ Sbjct: 1 MKFAVISKGDAISNECMYKIRTYLTDFRLTYDEDEPDIVISVGGDGTLLYAFHRYRSRLE 60 Query: 61 NLAFVGVHTGHLGFYADWKADELPALADYMCGTGSAEPHKPRIVKYPLLELEIHKKSG-- 118 AFVGVHTGHLGFYADW DE+ L + T P++ +++YPLLE+ I G Sbjct: 61 QTAFVGVHTGHLGFYADWVPDEIEKLVIAIAKT----PYQ--VIEYPLLEVTIRYVGGGR 114 Query: 119 SSSHIALNEFTLKGVDGTVVIQVDINDVTFEMFRGDGLCVSTPSGSTAYNKSLGGAMVHP 178 + ++ALNE T+K V GT+V+ V+I FE FRGDGLC+STPSGSTAYNK+LGGA++HP Sbjct: 115 ETKYLALNECTVKSVSGTLVMDVEIRGDLFETFRGDGLCISTPSGSTAYNKALGGAILHP 174 Query: 179 TIEALQIAEIASINNRVFRTMGSPLLLPKHHHCDIFSRKDQRLLLTIDHNNIPVDDLISV 238 ++EA+Q+AE+ASINNRVFRT+GSPL+LP HH C + D +TIDH ++ D+ S+ Sbjct: 175 SLEAIQVAEMASINNRVFRTIGSPLILPAHHTCMLKPVNDVDFQITIDHLSLLHKDVKSI 234 Query: 239 RCQVSDKKISFARYRPFPFWNRVREAFL 266 +C+V+D+KI FAR+RPFPFW RVRE+F+ Sbjct: 235 QCRVADEKIRFARFRPFPFWKRVRESFI 262 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.323 0.139 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,616,490,601 Number of extensions: 67955199 Number of successful extensions: 147475 Number of sequences better than 1.0e-05: 1631 Number of HSP's gapped: 145014 Number of HSP's successfully gapped: 1631 Length of query: 267 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 134 Effective length of database: 1,807,701,208 Effective search space: 242231961872 Effective search space used: 242231961872 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)