BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0420.0017.p (413 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KKR1.1 B3KKR1_9BACI Sporulation integral membrane protein YlbJ 408 e-112 B1DFD4.1 B1DFD4_9BACL Sporulation integral membrane protein YlbJ 342 4e-92 C0ZFQ6.1 C0ZFQ6_BREBN Conserved hypothetical membrane protein 289 3e-76 C1P716.1 C1P716_BACCO Sporulation integral membrane protein YlbJ 279 4e-73 B7GGK1.1 B7GGK1_ANOFW Uncharacterized protein conserved in bacteria 278 6e-73 >B3KKR1.1 B3KKR1_9BACI Sporulation integral membrane protein YlbJ Length = 405 Score = 408 bits (1048), Expect = e-112, Method: Compositional matrix adjust. Identities = 205/326 (62%), Positives = 245/326 (75%), Gaps = 1/326 (0%) Query: 26 PASSLDAALRGLSVWWDVLFPSLFPFFVISEMMLGFGVVHLFGALLDPLMRPLFNIPGSG 85 P ++L +ALRGL++WWDVLFPSLFPFFVISE++LGFGVVHL G LLDP+MRPLF IPG+G Sbjct: 26 PEAALQSALRGLAIWWDVLFPSLFPFFVISEVLLGFGVVHLIGTLLDPMMRPLFRIPGAG 85 Query: 86 GFVAAMGYVSGYPVGARLTAKLREQGLLNRVEGERLVAFTTSSDPIFLLGAVSVGFFHDA 145 GFVAAMGYVSGYPVGA+LTAKL EQ ++NR EGERLVAFTTSSDPIFL+GAVS+GFFHD Sbjct: 86 GFVAAMGYVSGYPVGAKLTAKLWEQKMVNREEGERLVAFTTSSDPIFLIGAVSIGFFHDP 145 Query: 146 SLGLILALAHYGGGLIVGLLMSFHGRXXXXXXXXXXXXXXXERAGRLRSALSSMAEARRR 205 +GLILALAHYGGG +VGLLM FHG+ + RL+SA +M AR + Sbjct: 146 KIGLILALAHYGGGFLVGLLMRFHGK-RESNTSTDSLLLSQTKTSRLKSAFQAMHAAREQ 204 Query: 206 DGRSVGELLKGAVQSSLQXXXXXXXXXXXXNVLMEMMDRAGIMSALFSFTGRLLSLAGFP 265 DGR +G LL+ A+QSSLQ +V +E+ RAG+M AL LLSL G P Sbjct: 205 DGRDLGTLLRQAIQSSLQLIIVVGGLVVFFSVFLELWTRAGVMPALTEVISLLLSLVGMP 264 Query: 266 AELSTALVSGLFEVTLGTRSAGAAAASVPLEFKAAAGAWVLSWGGLSVHAQVASILNGTG 325 LSTA+V GLFEVTLG R AG A A++PL +KAAA A++LSWGGLSVHAQ+ SILN T Sbjct: 265 ESLSTAIVGGLFEVTLGARYAGEAGAAIPLPYKAAAAAFILSWGGLSVHAQIISILNSTN 324 Query: 326 LRYLPFLAARLVHAFLSTGLVLLLWK 351 LRY PF+ ARL+H ++ VLLLW+ Sbjct: 325 LRYFPFVVARLIHGIIAATAVLLLWR 350 >B1DFD4.1 B1DFD4_9BACL Sporulation integral membrane protein YlbJ Length = 352 Score = 342 bits (877), Expect = 4e-92, Method: Compositional matrix adjust. Identities = 187/325 (57%), Positives = 231/325 (71%), Gaps = 4/325 (1%) Query: 26 PASSLDAALRGLSVWWDVLFPSLFPFFVISEMMLGFGVVHLFGALLDPLMRPLFNIPGSG 85 P+ +L++ALRGLS+WW VLFP+LFPFFVISE++LGFG+VH FG LLDPLMRPLF +PG G Sbjct: 27 PSQTLESALRGLSIWWQVLFPALFPFFVISELLLGFGIVHFFGKLLDPLMRPLFRLPGIG 86 Query: 86 GFVAAMGYVSGYPVGARLTAKLREQGLLNRVEGERLVAFTTSSDPIFLLGAVSVGFFHDA 145 FV MGYVSGYPVGARLTA+L EQGL+ R EGERLVAFTT+SDPIFL+GAVSVGFFH+A Sbjct: 87 SFVVTMGYVSGYPVGARLTAQLWEQGLVKRAEGERLVAFTTTSDPIFLIGAVSVGFFHNA 146 Query: 146 SLGLILALAHYGGGLIVGLLMSFHGRXXXXXXXXXXXXXXXERAGRLRSALSSMAEARRR 205 +L +LA+AHYGG I+GLLM FH R L A +M EAR Sbjct: 147 ALAPVLAVAHYGGAFIIGLLMRFHDRHEPVTESLRRAPSRRSSR--LVEATKAMHEARLL 204 Query: 206 DGRSVGELLKGAVQSSLQXXXXXXXXXXXXNVLMEMMDRAGIMSALFSFTGRLLSLAGFP 265 DGRS+G+LL+ ++QS+L+ +V+ME++ AG++ AL L G P Sbjct: 205 DGRSLGKLLQDSIQSALRLMMVVGGLVVFFSVVMELLTHAGVVEALAEGLRLLFGAVGLP 264 Query: 266 AELSTALVSGLFEVTLGTRSAGAAAASVPLEFKAAAGAWVLSWGGLSVHAQVASILNGTG 325 A+L+ A+V GLFEVTLG R+AG A L +AA AW+LSWGGLSVHAQV S+L+ T Sbjct: 265 AKLADAIVFGLFEVTLGARAAGEAGTG--LMHQAAIAAWILSWGGLSVHAQVLSLLSRTD 322 Query: 326 LRYLPFLAARLVHAFLSTGLVLLLW 350 LRY+P + ARL HAF+S LV LLW Sbjct: 323 LRYMPLMLARLAHAFISVALVYLLW 347 >C0ZFQ6.1 C0ZFQ6_BREBN Conserved hypothetical membrane protein Length = 410 Score = 289 bits (740), Expect = 3e-76, Method: Compositional matrix adjust. Identities = 154/330 (46%), Positives = 209/330 (63%), Gaps = 5/330 (1%) Query: 29 SLDAALRGLSVWWDVLFPSLFPFFVISEMMLGFGVVHLFGALLDPLMRPLFNIPGSGGFV 88 S +AA+RGL +WW+V+FPS PF V+SE+++G GVVH G LL+PLMRPLFN+PG+GGFV Sbjct: 30 SFEAAVRGLKIWWEVVFPSTLPFIVLSEVLMGLGVVHFVGVLLEPLMRPLFNVPGTGGFV 89 Query: 89 AAMGYVSGYPVGARLTAKLREQGLLNRVEGERLVAFTTSSDPIFLLGAVSVGFFHDASLG 148 AMG+ SGYPV A+LT +LR QG + + EGERLV+FTT+ DP+F++GAV++GFFH +G Sbjct: 90 LAMGFSSGYPVAAKLTTRLRLQGNVTKAEGERLVSFTTTGDPLFVMGAVAIGFFHSEQMG 149 Query: 149 LILALAHYGGGLIVGLLMSFHGRXXXXXXXXXXXXXXXERAGRLRSALSSMAEARRRDGR 208 +ILAL HY +++G++ FH LR AL +M AR RD R Sbjct: 150 VILALTHYLSAVLMGVIYRFHAPFAITSAPLEKNSLPLP----LR-ALQAMHRARIRDRR 204 Query: 209 SVGELLKGAVQSSLQXXXXXXXXXXXXNVLMEMMDRAGIMSALFSFTGRLLSLAGFPAEL 268 G+L+ AVQS+L +VL+++ + + + L GFP Sbjct: 205 PFGKLMGEAVQSALNTLLMIGGFIIVFSVLIQLFSTIHLTQIISTLLSIFLGPFGFPPAF 264 Query: 269 STALVSGLFEVTLGTRSAGAAAASVPLEFKAAAGAWVLSWGGLSVHAQVASILNGTGLRY 328 S A+V+GLFEVTLG ++A V L +KAA + +LSWGGLSVHAQVASIL+ T +R Sbjct: 265 SQAIVAGLFEVTLGAQAASIVPNDVSLVWKAAIASAILSWGGLSVHAQVASILSETDIRV 324 Query: 329 LPFLAARLVHAFLSTGLVLLLWKPFAHSGS 358 P+L AR +HA L+ L +LW P A S Sbjct: 325 APYLIARSLHALLAAILTFVLWGPLATVSS 354 >C1P716.1 C1P716_BACCO Sporulation integral membrane protein YlbJ Length = 406 Score = 279 bits (714), Expect = 4e-73, Method: Compositional matrix adjust. Identities = 149/330 (45%), Positives = 210/330 (63%), Gaps = 7/330 (2%) Query: 23 IMNPASSLDAALRGLSVWWDVLFPSLFPFFVISEMMLGFGVVHLFGALLDPLMRPLFNIP 82 I+ P S A+++GL++WW+V+FPSLFPFFVISE+++GFGVV G LL+PLMRPLF +P Sbjct: 23 IVLPDESFKASIQGLNMWWEVVFPSLFPFFVISELLIGFGVVRFVGVLLEPLMRPLFRVP 82 Query: 83 GSGGFVAAMGYVSGYPVGARLTAKLREQGLLNRVEGERLVAFTTSSDPIFLLGAVSVGFF 142 G GGF AMG SG P GA+LTA+LR++ ++RVE ERLVAFT+SS P+F+ GAV+VGFF Sbjct: 83 GVGGFTWAMGLASGNPSGAKLTARLRQEKQIDRVEAERLVAFTSSSSPLFIFGAVAVGFF 142 Query: 143 HDASLGLILALAHYGGGLIVGLLMSFHGRXXXXXXXXXXXXXXXERAGRLRSALSSMAEA 202 H+ SLG++ A AHY G +IVGL+M F+G + L+ AL + Sbjct: 143 HNPSLGILFAAAHYAGNIIVGLVMRFYG------TGEDIRGPKKKHTFLLKEALLVLHAT 196 Query: 203 RRRDGRSVGELLKGAVQSSLQXXXXXXXXXXXXNVLMEMMDRAGIMSALFSFTGRLLSLA 262 R ++ + +G+LL AV S++Q +V +++ + S +LL LA Sbjct: 197 RIKNSKPIGKLLGDAVMSAIQTLLMIGGFIILFSVFNKLLLYMHVTSVFGMLVSQLLHLA 256 Query: 263 GFPAELSTALVSGLFEVTLGTRSAGAAAASVPLEFKAAAGAWVLSWGGLSVHAQVASILN 322 F +LS L+SGLFEVTLG + S L+ + A +++L +GG S+ AQVASIL Sbjct: 257 HFSEKLSIPLISGLFEVTLGIQMISEVQESSLLQ-QVIAASFILGFGGFSIQAQVASILA 315 Query: 323 GTGLRYLPFLAARLVHAFLSTGLVLLLWKP 352 T +R+ PF ARL+H FLS ++ L+W P Sbjct: 316 QTDIRFRPFFLARLLHGFLSAVIIRLVWNP 345 >B7GGK1.1 B7GGK1_ANOFW Uncharacterized protein conserved in bacteria Length = 369 Score = 278 bits (712), Expect = 6e-73, Method: Compositional matrix adjust. Identities = 142/319 (44%), Positives = 203/319 (63%), Gaps = 8/319 (2%) Query: 34 LRGLSVWWDVLFPSLFPFFVISEMMLGFGVVHLFGALLDPLMRPLFNIPGSGGFVAAMGY 93 +RGL +WWDV+FPSL PFF++SE+++GFGVVH G LL+P MRPLF +PG GGFV AMG Sbjct: 1 MRGLHMWWDVVFPSLLPFFIVSEILIGFGVVHFLGVLLEPFMRPLFKVPGIGGFVWAMGM 60 Query: 94 VSGYPVGARLTAKLREQGLLNRVEGERLVAFTTSSDPIFLLGAVSVGFFHDASLGLILAL 153 SGYP GA+LTA+LR+Q L+ +E ERLV+FT SS+P+F+ GA+SVGFFH+ ++G+ILAL Sbjct: 61 ASGYPAGAKLTARLRQQQQLSAIEAERLVSFTNSSNPLFIFGAISVGFFHNPNIGIILAL 120 Query: 154 AHYGGGLIVGLLMSFHGRXXXXXXXXXXXXXXXERAGRLRSALSSMAEARRRDGRSVGEL 213 +HY G + VGL+M F+G+ + LR A +M E R + +++G+L Sbjct: 121 SHYLGNICVGLMMRFYGK-------DENDAVQKQTKSLLRQAFRAMHETRLKHSQAIGKL 173 Query: 214 LKGAVQSSLQXXXXXXXXXXXXNVLMEMMDRAGIMSALFSFTGRLLSLAGFPAELSTALV 273 L AV+SS+Q +VL +++ I L + P+ELS + Sbjct: 174 LGDAVRSSIQTLFMIGGFIILFSVLNKLLYITNITKWLADIVQYAFAFFHLPSELSVPFI 233 Query: 274 SGLFEVTLGTRSAGAAAASVPLEFKAAAGAWVLSWGGLSVHAQVASILNGTGLRYLPFLA 333 +GLFE+TLG++ A A + L+ K +++L++GG SV AQVASIL +R+ PF Sbjct: 234 AGLFEITLGSQMASEATNATLLQ-KVIITSFILAFGGFSVQAQVASILAEAHIRFQPFFI 292 Query: 334 ARLVHAFLSTGLVLLLWKP 352 AR+ H F + +LWKP Sbjct: 293 ARIFHGFFAAFFAFILWKP 311 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.325 0.140 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,857,430,623 Number of extensions: 69351454 Number of successful extensions: 281959 Number of sequences better than 1.0e-05: 185 Number of HSP's gapped: 281707 Number of HSP's successfully gapped: 188 Length of query: 413 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 275 Effective length of database: 1,760,596,133 Effective search space: 484163936575 Effective search space used: 484163936575 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)