BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0420.0004.p (332 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DJ49.1 B1DJ49_9BACL Membrane-bound metal-dependent hydrolase 356 2e-96 Q2B352.1 Q2B352_9BACI Putative uncharacterized protein 246 2e-63 Q5WIF9.1 Q5WIF9_BACSK Putative uncharacterized protein 246 3e-63 B7GII8.1 B7GII8_ANOFW Membrane-bound metal-dependent hydrolase 246 3e-63 A8FB76.1 A8FB76_BACP2 Possible membrane bound metal dependent hy... 239 2e-61 >B1DJ49.1 B1DJ49_9BACL Membrane-bound metal-dependent hydrolase Length = 328 Score = 356 bits (913), Expect = 2e-96, Method: Compositional matrix adjust. Identities = 173/328 (52%), Positives = 225/328 (68%), Gaps = 2/328 (0%) Query: 1 MDTATHFVMGLGLAGLSFVDPVVASQPTLAGAVMVATVLASQAPDADTALRLKDNALYIR 60 MDT TH VMG+GLAGL+ +DPVVA+ ++ AV+V T++ SQAPD DT LRLK NA YIR Sbjct: 1 MDTGTHLVMGIGLAGLACIDPVVANNSSIFTAVLVGTIIGSQAPDFDTFLRLKGNASYIR 60 Query: 61 NHRGITHSLPFLLLWPALITLVIGPVFGFTELHDLSHIALWSFIGVAVHVFTDLFNTYGT 120 NHRGI+HSLP + +W LIT ++ +F L L H+ W + V +HVF+DLFN YGT Sbjct: 61 NHRGISHSLPAIAIWTILITGLLQLIFR-GNLPWL-HVGSWVLLAVCLHVFSDLFNAYGT 118 Query: 121 QAARPFTEKWIAWNIIHIFDPFIFGSHVAAIILWISGFVPPAPLFITLYACIALYYIWRT 180 QA RPFTEKWI+WNIIHIFDP IF +HV AI++W G P +F LY +YY WRT Sbjct: 119 QAMRPFTEKWISWNIIHIFDPVIFTTHVIAILIWAFGIASPVAIFPVLYLFTIIYYTWRT 178 Query: 181 LVHARLTRKLKTKDIHHSPGERYFVIPTISPARWNVVKAKPDGSYIIGLLNSGRLEWFKH 240 L H R+L + D + Y IPTIS + WN+VK DGS++IG L +L W + Sbjct: 179 LAHRSTLRRLHSLDPEPDGTQTYIAIPTISLSNWNIVKRASDGSFVIGDLRGNKLRWLDN 238 Query: 241 AVCSTHPAVEHSKSHPDIKAFLYFTSYAVAEVEELPSGYIVRWGDVRYLHRKQFPFVAVL 300 CS HPAV S++ I++FLYFT +A AEV+E P G+ VRW DVRY HRKQ+PFVAVL Sbjct: 239 VRCSDHPAVAKSRNDNAIRSFLYFTKFACAEVKEHPWGFEVRWSDVRYRHRKQYPFVAVL 298 Query: 301 VMDSQYHPLNTYVGWLSSEKLDERFAID 328 +M+ +Y + +YVGWLS ++L +R ++ Sbjct: 299 LMNKEYETVGSYVGWLSDDRLMKRLRMN 326 >Q2B352.1 Q2B352_9BACI Putative uncharacterized protein Length = 354 Score = 246 bits (628), Expect = 2e-63, Method: Compositional matrix adjust. Identities = 137/335 (40%), Positives = 198/335 (59%), Gaps = 14/335 (4%) Query: 1 MDTATHFVMGLGLAGLSFVDPVVASQPTLAGAVMVATVLASQAPDADTALRLKDNALYIR 60 +DT TH VMG L GL+ +DPVVA A +VM+ ++ SQAPD DT L+L++NA+YIR Sbjct: 29 LDTGTHVVMGFALGGLATLDPVVADSTATATSVMIGALIGSQAPDIDTVLKLRNNAVYIR 88 Query: 61 NHRGITHSLPFLLLWPALITLVIGPVFGFTELHDLSHIALWSFIGVAVHVFTDLFNTYGT 120 NHRGITHS+P ++LWP I VI P F +L H+ +W+F V +HVF D+FN+YGT Sbjct: 89 NHRGITHSIPAVILWPLAILAVIYPFF---PEANLLHLWIWTFGAVFLHVFVDIFNSYGT 145 Query: 121 QAARPFTEKWIAWNIIHIFDPFIFGSHVAAIILWISGFVPPAPLFITLYACIALYYIWRT 180 QA RPF+ KW+A II+ FDPFIFG HVA + +WI G P F+ +YA + YYI R Sbjct: 146 QALRPFSSKWVALGIINTFDPFIFGIHVAGLFIWILG-AHPGYTFLAIYAVLIAYYIIRF 204 Query: 181 LVHARLTRKLKTKDIHHSPGERYFVI-PTISPARWNVVKAKPDGSYIIGLLNSGR---LE 236 L + + +K P +I PT+ +W + + + +G N+G L+ Sbjct: 205 LAQRAVLKAVKAI----IPDSTEIIIAPTMKFHQWR-LAIMTEHQFFVGRANNGHIDILD 259 Query: 237 WFKHAVCSTHPAVEHSKSHPDIKAFLYFTSYAVAEVEELPSGYIVRWGDVRYLHRKQFPF 296 F P +E +K ++ AFL F+ EVE+L + VR+ D+RY +PF Sbjct: 260 QFNRVPVPQTPVLEAAKKDKNLSAFLSFSPVYRWEVEDLEESFEVRFIDLRYRSNGHYPF 319 Query: 297 VAVLVMDSQYHPLNTYVGWL-SSEKLDERFAIDPG 330 VAV+ +D+ + +++Y GW+ S EKL ++ I P Sbjct: 320 VAVVQLDNDLNIISSYTGWIFSEEKLRKKLDIIPN 354 >Q5WIF9.1 Q5WIF9_BACSK Putative uncharacterized protein Length = 326 Score = 246 bits (627), Expect = 3e-63, Method: Compositional matrix adjust. Identities = 135/327 (41%), Positives = 199/327 (60%), Gaps = 10/327 (3%) Query: 1 MDTATHFVMGLGLAGLSFVDPVVASQPTLAGAVMVATVLASQAPDADTALRLKDNALYIR 60 MDT+TH VMG GLAGL+ +D +AS P + AV+ T++AS APD DT L+LK+NA+YIR Sbjct: 1 MDTSTHIVMGAGLAGLATLDVQIASSPYMMTAVLATTLVASNAPDFDTVLKLKNNAIYIR 60 Query: 61 NHRGITHSLPFLLLWPALITLVIGPVFGFTELHDLSHIALWSFIGVAVHVFTDLFNTYGT 120 +HRG+THS+P LLLWP +I+ G + F + I LW+ I V +HVF D+FN YGT Sbjct: 61 HHRGMTHSIPALLLWPFIIS---GGMMLFFQDAYWPTILLWAAISVFLHVFVDIFNAYGT 117 Query: 121 QAARPFTEKWIAWNIIHIFDPFIFGSHVAAIILWISGFVPPAPLFITLYACIALYYIWRT 180 QA PF+ +WIA +++IFDP +FG+HV I+LW GF PP P F+ +Y + LYY WR Sbjct: 118 QALWPFSNRWIALGVMNIFDPVVFGAHVVGILLWALGF-PPGPSFVVIYVGLVLYYAWRI 176 Query: 181 LVHARLTRKLKTKDIHHSPGERYFVIPTISPARWNVVKAKPDGSYIIGLLNSGRLEWFKH 240 + ++ + HH FV PTI +W++V PD Y+ N + ++ Sbjct: 177 SEKRDVVKRARQ---HHPNATHIFVSPTIRWNKWHLVIRTPDMLYVAQSKNKKITFFERY 233 Query: 241 AV--CSTHPAVEHSKSHPDIKAFLYFTSYAVAEVEELPS-GYIVRWGDVRYLHRKQFPFV 297 A T P ++ + S ++ AFL F+ E+E GY VR+ D+RY + +PFV Sbjct: 234 AFDPIPTDPVMQAACSDKNLAAFLSFSPTYRWEMEATTDGGYEVRFFDLRYRSKGHYPFV 293 Query: 298 AVLVMDSQYHPLNTYVGWLSSEKLDER 324 A++ +D+ + + +Y GW+ SE+ +R Sbjct: 294 AIVQLDADLNIVTSYTGWVYSEEALQR 320 >B7GII8.1 B7GII8_ANOFW Membrane-bound metal-dependent hydrolase Length = 325 Score = 246 bits (627), Expect = 3e-63, Method: Compositional matrix adjust. Identities = 137/334 (41%), Positives = 200/334 (59%), Gaps = 13/334 (3%) Query: 1 MDTATHFVMGLGLAGLSFVDPVVASQPTLAGAVMVATVLASQAPDADTALRLKDNALYIR 60 MDT TH +MG L GL+ DP VA+ PTLA AV++ T+L SQAPD DT L+LK+NA YIR Sbjct: 1 MDTGTHVLMGFALGGLATFDPAVANDPTLAHAVLIGTLLGSQAPDIDTVLKLKNNAKYIR 60 Query: 61 NHRGITHSLPFLLLWPALITLVIGPVFGFTELHDLSHIALWSFIGVAVHVFTDLFNTYGT 120 NHRG+THS+P L +W LI++ + V+ + H+ALW++I V++HVF D+FN+YGT Sbjct: 61 NHRGMTHSIPALFVWTLLISVALFYVYDTS----FFHVALWTWIAVSLHVFVDIFNSYGT 116 Query: 121 QAARPFTEKWIAWNIIHIFDPFIFGSHVAAIILWISGFVPPAPLFITLYACIALYYIWRT 180 QA RPF++KW+A II+ FDPFIF H+A ++LW+SG F+++Y + YYI R Sbjct: 117 QALRPFSKKWVALGIINTFDPFIFIIHLAGVVLWLSGH-HAGYTFLSVYIVLIGYYIIRF 175 Query: 181 LVHARLTRKLKTKDIHHSPGERYFVIPTISPARWNVVKAKPDGSYIIGLLNSGR---LEW 237 + RKLK S E+ +PTI W++ Y + SG L+ Sbjct: 176 RQQQAIKRKLKQM---FSDVEQIITVPTIKWNEWHLAVTTSTHFY-VARAQSGAIDVLDV 231 Query: 238 FKHAVCSTHPAVEHSKSHPDIKAFLYFTSYAVAEVEELPSGYIVRWGDVRYLHRKQFPFV 297 F+ P +E +K ++ AFL F+ E+ E Y VR+ D+RY + +PFV Sbjct: 232 FEKCPVPNIPVIEKAKQDENLAAFLSFSPVYRWEIHEYDDHYEVRFIDLRYRSKDYYPFV 291 Query: 298 AVLVMDSQYHPLNTYVGWLSSE-KLDERFAIDPG 330 AV+ +D + +++Y GW+ SE KL ++ + P Sbjct: 292 AVVQLDRDLNIVSSYTGWIFSEKKLRKKLELIPS 325 >A8FB76.1 A8FB76_BACP2 Possible membrane bound metal dependent hydrolase Length = 326 Score = 239 bits (611), Expect = 2e-61, Method: Compositional matrix adjust. Identities = 139/331 (41%), Positives = 199/331 (60%), Gaps = 12/331 (3%) Query: 1 MDTATHFVMGLGLAGLSFVDPVVASQPTLAGAVMVATVLASQAPDADTALRLKDNALYIR 60 MDT TH VMG+ L GL+ +DP V++ P +A AVM+AT+ SQAPD DT L+LK+NA+YIR Sbjct: 1 MDTGTHVVMGISLGGLALLDPAVSADPHMAHAVMIATIAGSQAPDIDTVLKLKNNAVYIR 60 Query: 61 NHRGITHSLPFLLLWPALITLVIGPVFGFTELHDLSHIALWSFIGVAVHVFTDLFNTYGT 120 NHRG THS+P +L W LI VI P F DL H+ LW+ + V +HVF D+FN YGT Sbjct: 61 NHRGFTHSIPAVLFWSLLIPAVIFP---FVPGADLLHLWLWTLLAVVLHVFVDIFNAYGT 117 Query: 121 QAARPFTEKWIAWNIIHIFDPFIFGSHVAAIILWISGFVPPAPLFITLYACIALYYIWRT 180 QA RPF++KW+A II+ FDPFIF +H+ AI++W G P FITLYA I YYI R Sbjct: 118 QAIRPFSKKWVALGIINTFDPFIFFTHLVAIVVWAIG-GHPGYTFITLYAVIFCYYIVRV 176 Query: 181 LVHARLTRKLKTKDIHHSPGERYFVIPTISPARWNVVKAKPDGSYIIGLLNSGR---LEW 237 ++ ++ R+L+ + E + PT+ +W + K S+ +G +G L+ Sbjct: 177 IMQLQIKRQLRAR---FDEIEDIIISPTMKFRQWRIA-VKCKSSFHVGRSMNGEIIILDT 232 Query: 238 FKHAVCSTHPAVEHSKSHPDIKAFLYFTSYAVAEVEELPSGYIVRWGDVRYLHRKQFPFV 297 F ++ +K +I AFL F+ EV++ Y VR+ D+RY + +PFV Sbjct: 233 FNRVPVPDTEIMKIAKQDENISAFLSFSPVYRWEVDKYKDHYEVRFIDLRYRSKGHYPFV 292 Query: 298 AVLVMDSQYHPLNTYVGWL-SSEKLDERFAI 327 A+ +D + N+Y GW+ S EKL ++ + Sbjct: 293 AICHIDMDHQIKNSYTGWIFSEEKLQKKLRL 323 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.326 0.140 0.454 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,176,343,221 Number of extensions: 92605263 Number of successful extensions: 276569 Number of sequences better than 1.0e-05: 189 Number of HSP's gapped: 276205 Number of HSP's successfully gapped: 189 Length of query: 332 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 196 Effective length of database: 1,779,438,163 Effective search space: 348769879948 Effective search space used: 348769879948 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 131 (55.1 bits)