BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0417.0017.p (299 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DAY6.1 B1DAY6_9BACL RarD protein, DMT superfamily transporter 408 e-112 B3KEW9.1 B3KEW9_9BACI RarD protein, DMT superfamily transporter 391 e-107 Q2B3D0.1 Q2B3D0_9BACI Chloramphenicol-sensitive protein rarD 204 9e-51 C0ZGJ2.1 C0ZGJ2_BREBN Conserved hypothetical membrane protein 204 1e-50 C1PFQ9.1 C1PFQ9_BACCO RarD protein, DMT superfamily transporter 193 1e-47 >B1DAY6.1 B1DAY6_9BACL RarD protein, DMT superfamily transporter Length = 292 Score = 408 bits (1049), Expect = e-112, Method: Compositional matrix adjust. Identities = 214/292 (73%), Positives = 231/292 (79%) Query: 8 MNNGLLNAIIAYIMWGVLPLYWKLFDNVPAGEILSHRVVWSFVFMGILITIQRRWGDMKR 67 MNNGL+NAIIAYIMWGVLPLYWKLF+NVPAGEILSHRVVWSFVFMGIL+ +QRRWGDMKR Sbjct: 1 MNNGLVNAIIAYIMWGVLPLYWKLFNNVPAGEILSHRVVWSFVFMGILVAVQRRWGDMKR 60 Query: 68 IAANRTHLQSLAASGLLIAVNWLIFIWAVNNGHVVETSXXXXXXXXXXXXXXXXXXHEKP 127 IA +R L LAASGLLIA NWLIFIWAVNNGHVVETS EKP Sbjct: 61 IATSRALLLPLAASGLLIAANWLIFIWAVNNGHVVETSLGYYLNPLLNVLLAVVFLREKP 120 Query: 128 NRGQWXXXXXXXXXXXXXXXDYGRFPWIAISLASSFGLYGLAKKKTVQVASVGLLSETAV 187 NRGQW DYGRFPW+AISLA+SFGLYG AKKK +Q ASVGLLSETAV Sbjct: 121 NRGQWLAIAIAGSAVLIIAIDYGRFPWVAISLAASFGLYGFAKKKIMQDASVGLLSETAV 180 Query: 188 VLPIALGYWIYLAAAGQSTAWTLPPSFFVELLLSGVVTXXXXXXXXXXXXXMSLSTLGFV 247 VLP+ALGYWIYLAAAG++TAWTLP S FVELLLSGVVT M+LS LGFV Sbjct: 181 VLPVALGYWIYLAAAGKTTAWTLPASTFVELLLSGVVTALPLLFFARAAARMALSALGFV 240 Query: 248 QYIGPTIMLLLSVFVFKETVSPVLLVGFALIWTALVVYAVSSVRGTKLAKAS 299 QYIGPTIML LS+FVFKE+VSPVLLVGFALIWT+LVVYAV+SVRGT+LAKAS Sbjct: 241 QYIGPTIMLFLSIFVFKESVSPVLLVGFALIWTSLVVYAVASVRGTRLAKAS 292 >B3KEW9.1 B3KEW9_9BACI RarD protein, DMT superfamily transporter Length = 292 Score = 391 bits (1005), Expect = e-107, Method: Compositional matrix adjust. Identities = 212/292 (72%), Positives = 232/292 (79%) Query: 8 MNNGLLNAIIAYIMWGVLPLYWKLFDNVPAGEILSHRVVWSFVFMGILITIQRRWGDMKR 67 MNNGL+NAIIAYIMWGVLPLYWKLF+NVPAGEILSHRVVW+FVFMGIL+ +QRRWGDMKR Sbjct: 1 MNNGLVNAIIAYIMWGVLPLYWKLFENVPAGEILSHRVVWTFVFMGILVAVQRRWGDMKR 60 Query: 68 IAANRTHLQSLAASGLLIAVNWLIFIWAVNNGHVVETSXXXXXXXXXXXXXXXXXXHEKP 127 +A +R+ L SLAASGLLIA NWLIFIWAVNNGHVVETS EKP Sbjct: 61 VATSRSLLLSLAASGLLIAANWLIFIWAVNNGHVVETSLGYYLNPLLNVLLAVVFLREKP 120 Query: 128 NRGQWXXXXXXXXXXXXXXXDYGRFPWIAISLASSFGLYGLAKKKTVQVASVGLLSETAV 187 +RGQW DYGRFPW+AISLA SFGLYGLAKKK VQ ASVGLLSETAV Sbjct: 121 SRGQWLGIAIAGAAVLIIAIDYGRFPWVAISLAVSFGLYGLAKKKIVQDASVGLLSETAV 180 Query: 188 VLPIALGYWIYLAAAGQSTAWTLPPSFFVELLLSGVVTXXXXXXXXXXXXXMSLSTLGFV 247 +LP+A+GYWIYLAA G++TAWTLP FVELLLSGVVT M+LSTLGFV Sbjct: 181 ILPVAIGYWIYLAAVGKTTAWTLPAPVFVELLLSGVVTALPLLFFARAAARMALSTLGFV 240 Query: 248 QYIGPTIMLLLSVFVFKETVSPVLLVGFALIWTALVVYAVSSVRGTKLAKAS 299 QYIGPTIMLLLS+FVFKE+VSPVLL+GFALIWTALVVYAV+SVR TKLAKAS Sbjct: 241 QYIGPTIMLLLSIFVFKESVSPVLLIGFALIWTALVVYAVASVRSTKLAKAS 292 >Q2B3D0.1 Q2B3D0_9BACI Chloramphenicol-sensitive protein rarD Length = 308 Score = 204 bits (519), Expect = 9e-51, Method: Compositional matrix adjust. Identities = 113/291 (38%), Positives = 163/291 (56%), Gaps = 4/291 (1%) Query: 5 ESKMNNGLLNAIIAYIMWGVLPLYWKLFDNVPAGEILSHRVVWSFVFMGILITIQRRWGD 64 ++++ G L A ++Y++WG+LP+YWKL ++V A EIL+ RV WSF+FM ++ + R+W D Sbjct: 3 DTEVKQGALYAGLSYLIWGILPIYWKLLNHVSADEILASRVFWSFIFMLAVLVLTRKWPD 62 Query: 65 MKR----IAANRTHLQSLAASGLLIAVNWLIFIWAVNNGHVVETSXXXXXXXXXXXXXXX 120 R I N+ + +L + LLI+ NW I+IWAVN+G ++E S Sbjct: 63 FVRTLRGIRNNKKQMLALTIASLLISCNWFIYIWAVNSGQMIEASLGYYINPLVSVLLGI 122 Query: 121 XXXHEKPNRGQWXXXXXXXXXXXXXXXDYGRFPWIAISLASSFGLYGLAKKKTVQVASVG 180 EK + Q YGRFPWIA+ LA SFGLYGLAKK +++G Sbjct: 123 VVLKEKLSSVQAVSFLLAAAGVLIMTLSYGRFPWIAVVLALSFGLYGLAKKLIKVESAIG 182 Query: 181 LLSETAVVLPIALGYWIYLAAAGQSTAWTLPPSFFVELLLSGVVTXXXXXXXXXXXXXMS 240 L ET VV+P+ALGY IYL AAG+ + + + L+ +G T + Sbjct: 183 LTLETMVVMPLALGYLIYLFAAGKQSFLAISTGTDLLLIGAGAATAIPLLYFAKGAQKIP 242 Query: 241 LSTLGFVQYIGPTIMLLLSVFVFKETVSPVLLVGFALIWTALVVYAVSSVR 291 LS LG++QYI PT+ LLL +FV+ E + LV F IW AL VY++S + Sbjct: 243 LSMLGYLQYIAPTLTLLLGIFVYGEEFTKTHLVAFLFIWLALTVYSLSRTK 293 >C0ZGJ2.1 C0ZGJ2_BREBN Conserved hypothetical membrane protein Length = 302 Score = 204 bits (518), Expect = 1e-50, Method: Compositional matrix adjust. Identities = 116/284 (40%), Positives = 153/284 (53%) Query: 8 MNNGLLNAIIAYIMWGVLPLYWKLFDNVPAGEILSHRVVWSFVFMGILITIQRRWGDMKR 67 M G++ AI+AY+ WG+LPLYWKLF + A EIL+HR+VWS +F+ I+I + +RW M Sbjct: 1 MRQGIIYAIVAYLAWGLLPLYWKLFQAMGAWEILAHRIVWSVIFVAIIIQVTKRWRSMWG 60 Query: 68 IAANRTHLQSLAASGLLIAVNWLIFIWAVNNGHVVETSXXXXXXXXXXXXXXXXXXHEKP 127 +LA LLI+ NWLIFIWAVNN HV++TS EK Sbjct: 61 AVTGFKMFGALAICSLLISANWLIFIWAVNNNHVMQTSLGYYMNPLITVLLGVLFLKEKM 120 Query: 128 NRGQWXXXXXXXXXXXXXXXDYGRFPWIAISLASSFGLYGLAKKKTVQVASVGLLSETAV 187 + GQW YG PW+++SLA +F Y LAKK A VGL ET Sbjct: 121 HAGQWFALILAAFGVLFITFQYGEIPWVSLSLALTFAFYSLAKKLVRLEAMVGLAWETLF 180 Query: 188 VLPIALGYWIYLAAAGQSTAWTLPPSFFVELLLSGVVTXXXXXXXXXXXXXMSLSTLGFV 247 V PIA GY + L G T +L + L L+GV T + LS +GF+ Sbjct: 181 VAPIAFGYLLMLQVNGTETMTSLAWWQLLLLTLAGVATAMPLYWFAQAATRLPLSVVGFI 240 Query: 248 QYIGPTIMLLLSVFVFKETVSPVLLVGFALIWTALVVYAVSSVR 291 QY+ PTI LL +VF+F E + + F IW+AL+V+ VSSVR Sbjct: 241 QYLAPTISLLSAVFLFGEPFTQTHFISFGFIWSALIVFTVSSVR 284 >C1PFQ9.1 C1PFQ9_BACCO RarD protein, DMT superfamily transporter Length = 317 Score = 193 bits (491), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 112/293 (38%), Positives = 155/293 (52%), Gaps = 4/293 (1%) Query: 6 SKMNNGLLNAIIAYIMWGVLPLYWKLFDNVPAGEILSHRVVWSFVFMGILITIQRRWGDM 65 + +G+L AY +WG+ P+YWK+ + VPA EIL+HR+ WSF+FM IL+ R+ GD+ Sbjct: 17 NNQKSGVLYTFFAYFLWGLFPIYWKMIEQVPAIEILAHRIFWSFIFMIILLAFLRKMGDL 76 Query: 66 K-RIAANRTHLQSLAASGLLIAV---NWLIFIWAVNNGHVVETSXXXXXXXXXXXXXXXX 121 K I H ++ L + NW I+IWAVNNGHV+ETS Sbjct: 77 KTSIKEGVRHPRTPLLLLLASVLVSGNWFIYIWAVNNGHVIETSMGYYINPLVSILLGMV 136 Query: 122 XXHEKPNRGQWXXXXXXXXXXXXXXXDYGRFPWIAISLASSFGLYGLAKKKTVQVASVGL 181 EK NR Q YG PWIAI LA +FG YGL KKK A +GL Sbjct: 137 FLKEKLNRWQCLAFLLAACGVAIQTFSYGHIPWIAIGLALTFGFYGLVKKKVDIDAFIGL 196 Query: 182 LSETAVVLPIALGYWIYLAAAGQSTAWTLPPSFFVELLLSGVVTXXXXXXXXXXXXXMSL 241 ET V+P+AL Y + + G ++ T+ P V L+ +GVVT +SL Sbjct: 197 TFETFFVMPVALLYLLVIQVKGTASFATVSPGLTVLLIGTGVVTAIPLICFAQGARSISL 256 Query: 242 STLGFVQYIGPTIMLLLSVFVFKETVSPVLLVGFALIWTALVVYAVSSVRGTK 294 + +GF QY+ PT+ L++ + FKE + V L+ FA IW AL+V++VS K Sbjct: 257 TMIGFFQYLTPTMTLIMGIAFFKEPFTHVQLLSFAFIWLALIVFSVSGFLPKK 309 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.325 0.138 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,425,088,509 Number of extensions: 47481502 Number of successful extensions: 181456 Number of sequences better than 1.0e-05: 793 Number of HSP's gapped: 179604 Number of HSP's successfully gapped: 820 Length of query: 299 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 164 Effective length of database: 1,788,859,178 Effective search space: 293372905192 Effective search space used: 293372905192 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 131 (55.1 bits)