BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0416.0008.p (182 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A0LFU3.1 A0LFU3_SYNFM Glutamine amidotransferase class-I 199 1e-49 Q0W1E4.1 Q0W1E4_UNCMA Glutamine amidotransferase (Class I) 184 2e-45 C1TA20.1 C1TA20_9FIRM GMP synthase family protein 181 2e-44 C0QPT3.1 C0QPT3_PERMH Glutamine amidotransferase class-I 178 2e-43 Q8PRW0.1 Q8PRW0_METMA Conserved protein 178 2e-43 >A0LFU3.1 A0LFU3_SYNFM Glutamine amidotransferase class-I Length = 233 Score = 199 bits (506), Expect = 1e-49, Method: Compositional matrix adjust. Identities = 94/177 (53%), Positives = 120/177 (67%), Gaps = 1/177 (0%) Query: 1 MGVYDEEKFPWLVREKTFIKETIRQRKMVLGICLGAQLIAEALGGKVYRNQYKEIGWHPV 60 M +Y+E+++PWLV EK FI E I + K+VLGICLGAQLI++ LGGKVYRN KEIGW PV Sbjct: 55 MNIYEEDRYPWLVEEKKFIAEAIEKNKIVLGICLGAQLISDVLGGKVYRNGEKEIGWFPV 114 Query: 61 KMTEESRSSVFFKRFPGEYVPFHWHGDTFALPDGVKTAAISLGCANQAFEYN-GHVIGLQ 119 +T E+ SV F P + FHWHGDTF L G A S CA QAFE N G V+GLQ Sbjct: 115 SLTPEAAESVVFSALPPRFTAFHWHGDTFHLAPGATRTARSDACAVQAFEANGGRVVGLQ 174 Query: 120 FHLESSNDSILKLIEHCGDEIEPGTYVQLPDQMMDQTSFLAHSNTILVHLLDTLDKN 176 FHLESS +SI LI++CGDE+ P Y+Q +++ Q +L +T + LLD + +N Sbjct: 175 FHLESSAESIRLLIDNCGDELAPAPYIQRKHEIVSQEHYLGEISTTMTTLLDAVKRN 231 >Q0W1E4.1 Q0W1E4_UNCMA Glutamine amidotransferase (Class I) Length = 234 Score = 184 bits (468), Expect = 2e-45, Method: Compositional matrix adjust. Identities = 89/179 (49%), Positives = 119/179 (66%), Gaps = 1/179 (0%) Query: 1 MGVYDEEKFPWLVREKTFIKETIRQRKMVLGICLGAQLIAEALGGKVYRNQYKEIGWHPV 60 M +Y+E+ +PWLVREK FIKE I K+VLG+CLGAQ+IA+ LG KV N+ KEIGW+ V Sbjct: 55 MNIYEEKDYPWLVREKKFIKEAIDAGKLVLGVCLGAQMIADVLGAKVTPNKEKEIGWYEV 114 Query: 61 KMTEESRSSVFFKRFPGEYVPFHWHGDTFALPDGVKTAAISLGCANQAFEYN-GHVIGLQ 119 T E ++S F P ++ FHWHGDTF +P G A S C NQAFEYN G V+GLQ Sbjct: 115 VKTPEGKASPVFGALPDRFIAFHWHGDTFGIPAGATRLAYSEACENQAFEYNGGKVLGLQ 174 Query: 120 FHLESSNDSILKLIEHCGDEIEPGTYVQLPDQMMDQTSFLAHSNTILVHLLDTLDKNNQ 178 FHLESS+DSI +L ++CGDE+ G ++Q +++ Q L + LLD ++K + Sbjct: 175 FHLESSDDSINRLAKNCGDELVDGKHIQKKAELIGQDEKLRTIYCHMCALLDAMEKAKK 233 >C1TA20.1 C1TA20_9FIRM GMP synthase family protein Length = 235 Score = 181 bits (460), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 89/174 (51%), Positives = 113/174 (64%), Gaps = 1/174 (0%) Query: 1 MGVYDEEKFPWLVREKTFIKETIRQRKMVLGICLGAQLIAEALGGKVYRNQYKEIGWHPV 60 M +Y+EE +PWL EK FI + I K VLGICLGAQLIA LG K+Y N+ KEIGW+PV Sbjct: 55 MNIYEEEIYPWLAAEKKFIAQAIAAGKQVLGICLGAQLIAAVLGAKIYGNENKEIGWYPV 114 Query: 61 KMTEESRSSVFFKRFPGEYVPFHWHGDTFALPDGVKTAAISLGCANQAFEYN-GHVIGLQ 119 +++E + F P + FHWHGDTF LP G AA S GC NQAF YN G VIGLQ Sbjct: 115 RISEGVQKLPVFSGLPQTFTAFHWHGDTFELPAGSVRAAHSEGCINQAFAYNDGKVIGLQ 174 Query: 120 FHLESSNDSILKLIEHCGDEIEPGTYVQLPDQMMDQTSFLAHSNTILVHLLDTL 173 FHLESS +S+ KL+++CGDE+ G Y+Q + D + + I+ LLD + Sbjct: 175 FHLESSLESVAKLVDNCGDELVGGKYIQSAAVIQDLDNNWSGIREIMARLLDNI 228 >C0QPT3.1 C0QPT3_PERMH Glutamine amidotransferase class-I Length = 230 Score = 178 bits (452), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 84/175 (48%), Positives = 113/175 (64%), Gaps = 1/175 (0%) Query: 1 MGVYDEEKFPWLVREKTFIKETIRQRKMVLGICLGAQLIAEALGGKVYRNQYKEIGWHPV 60 MGVYDE+++PWL EK FI+ +I+ K +LGICLGAQLIA+ LGGKVY+N+YKEIGW V Sbjct: 55 MGVYDEDEYPWLKDEKKFIERSIKSGKKILGICLGAQLIADVLGGKVYKNRYKEIGWFDV 114 Query: 61 KMTEESRSSVFFKRFPGEYVPFHWHGDTFALPDGVKTAAISLGCANQAFEYNGHVIGLQF 120 ++T E + S FF P + FHWHGDTF +PDG A S C NQAF Y V+GLQF Sbjct: 115 QLTSEGKKSKFFSDLPERFTAFHWHGDTFEIPDGAVHTARSEACENQAFVYEDRVVGLQF 174 Query: 121 HLESSNDSILKLIEHCGDEI-EPGTYVQLPDQMMDQTSFLAHSNTILVHLLDTLD 174 HLE+ + LIE+ +E+ E G Y+Q P+ ++ + +L +D + Sbjct: 175 HLETDLQTAKGLIENSEEELKEKGIYIQSPEYILSREDNFNTIEKLLYRFMDRFE 229 >Q8PRW0.1 Q8PRW0_METMA Conserved protein Length = 234 Score = 178 bits (451), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 80/176 (45%), Positives = 115/176 (65%), Gaps = 1/176 (0%) Query: 1 MGVYDEEKFPWLVREKTFIKETIRQRKMVLGICLGAQLIAEALGGKVYRNQYKEIGWHPV 60 M +Y+ +K+PWL EK F+++ + + K VLGICLGAQL+A+ L KV++N YKEIGW PV Sbjct: 55 MNIYEYDKYPWLKEEKKFLEKAVSEGKAVLGICLGAQLLADVLKAKVFKNDYKEIGWFPV 114 Query: 61 KMTEESRSSV-FFKRFPGEYVPFHWHGDTFALPDGVKTAAISLGCANQAFEYNGHVIGLQ 119 + ++ +S + + P ++ FHWHGDTF LP+G K S C NQ F Y VIGLQ Sbjct: 115 SVVKDGKSELSILEGMPEKFTAFHWHGDTFGLPEGAKRLFESQACKNQGFIYGDRVIGLQ 174 Query: 120 FHLESSNDSILKLIEHCGDEIEPGTYVQLPDQMMDQTSFLAHSNTILVHLLDTLDK 175 FHLE S SI +IE+C DE G Y+Q ++M+D+ +L S ++ L+D ++K Sbjct: 175 FHLEMSEQSIRNVIENCRDEFVEGKYIQRENEMLDRKDYLTESKKLMFRLMDNIEK 230 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.138 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,089,418,412 Number of extensions: 42359808 Number of successful extensions: 101269 Number of sequences better than 1.0e-05: 610 Number of HSP's gapped: 101280 Number of HSP's successfully gapped: 610 Length of query: 182 Length of database: 3,060,696,203 Length adjustment: 127 Effective length of query: 55 Effective length of database: 1,864,227,298 Effective search space: 102532501390 Effective search space used: 102532501390 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 127 (53.5 bits)