BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0416.0004.p (250 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DEI6.1 B1DEI6_9BACL Serine hydroxymethyltransferase EC=2.1.2.1 351 4e-95 B3K7K7.1 B3K7K7_9BACI Serine hydroxymethyltransferase EC=2.1.2.1 350 8e-95 C0Z819.1 C0Z819_BREBN Serine hydroxymethyltransferase EC=2.1.2.1 348 3e-94 B7GMG4.1 GLYA_ANOFW Serine hydroxymethyltransferase EC=2.1.2.1 344 5e-93 C3H9C1.1 C3H9C1_BACTU Serine hydroxymethyltransferase 339 1e-91 >B1DEI6.1 B1DEI6_9BACL Serine hydroxymethyltransferase EC=2.1.2.1 Length = 415 Score = 351 bits (900), Expect = 4e-95, Method: Compositional matrix adjust. Identities = 160/249 (64%), Positives = 198/249 (79%) Query: 1 MIVAGASAYPRTIEFEPFAQIAAEVGALFFVDMAHIAGIVAAGFHPSPIPHAHFVTTTTH 60 +IVAGASAYPRTI+FE QIA +VGALF VDMAHIAG+VAAG HP+P+PHAHFVTTTTH Sbjct: 166 LIVAGASAYPRTIDFEKLGQIAQDVGALFMVDMAHIAGLVAAGLHPNPVPHAHFVTTTTH 225 Query: 61 KTLRGPRGGIIMCRKPWAQAMDKAIFPGVQGGPLMHAIAAKTVAFGEALQPEFKSYIEKV 120 KTLRGPRGG+I+CRKPWA A+DKA+FPG QGGPLMH IAAK VAFGEALQP FK Y +KV Sbjct: 226 KTLRGPRGGMILCRKPWAAAIDKAVFPGSQGGPLMHIIAAKAVAFGEALQPSFKEYAQKV 285 Query: 121 LVNAKVLAEALLNEGLTVVSGGTDNHITLLDLRPIGLNGKEAESILDEVGITANKNAIPH 180 + NAKVL+E L+ EG+ +VSGGTDNH+ L+D R + ++G++AE +LD +GITANKNAIP Sbjct: 286 VSNAKVLSETLIAEGINIVSGGTDNHLMLIDTRSLNISGRDAEHVLDSIGITANKNAIPF 345 Query: 181 DTASPLITSGIRFGTPAMTTRGMGPQEMKEIARLIGLALKKPESSVAKEQLRRSVQEITS 240 D SP +TSGIR GTPA T+RGM MK I +I + LK P+ + V+++T+ Sbjct: 346 DPTSPFVTSGIRLGTPAATSRGMDESAMKTIGEIIAMTLKSPKDEAVLAKATGMVRDLTA 405 Query: 241 QFPLYEGLQ 249 Q+PLY ++ Sbjct: 406 QYPLYPNMK 414 >B3K7K7.1 B3K7K7_9BACI Serine hydroxymethyltransferase EC=2.1.2.1 Length = 416 Score = 350 bits (897), Expect = 8e-95, Method: Compositional matrix adjust. Identities = 160/245 (65%), Positives = 196/245 (80%) Query: 1 MIVAGASAYPRTIEFEPFAQIAAEVGALFFVDMAHIAGIVAAGFHPSPIPHAHFVTTTTH 60 +IVAGASAYPR I+FE A IA +VGALF VDMAHIAG+VAAG HP+P+PHAHFVTTTTH Sbjct: 167 LIVAGASAYPRIIDFEKLAAIANDVGALFMVDMAHIAGLVAAGLHPNPVPHAHFVTTTTH 226 Query: 61 KTLRGPRGGIIMCRKPWAQAMDKAIFPGVQGGPLMHAIAAKTVAFGEALQPEFKSYIEKV 120 KTLRGPRGG+I+C+K WAQA+DKA+FPG QGGPLMH IA+K VA GEAL P FK+Y E V Sbjct: 227 KTLRGPRGGMILCKKAWAQAIDKAVFPGSQGGPLMHVIASKAVALGEALDPSFKTYAENV 286 Query: 121 LVNAKVLAEALLNEGLTVVSGGTDNHITLLDLRPIGLNGKEAESILDEVGITANKNAIPH 180 + NAKVLA+ L+ EGL +VSGGTDNH+ L+D R + + GK+AE +LD +GIT NKNAIP Sbjct: 287 VKNAKVLADTLIEEGLNIVSGGTDNHLMLVDTRNLDITGKDAEKVLDSIGITVNKNAIPF 346 Query: 181 DTASPLITSGIRFGTPAMTTRGMGPQEMKEIARLIGLALKKPESSVAKEQLRRSVQEITS 240 D SP +TSGIR GTPA+T+RGM Q M +IA++I + LK+P+ E+ R V E+T Sbjct: 347 DPTSPFVTSGIRIGTPAVTSRGMDEQAMVKIAKIIAMTLKQPKDEATLEKAGRLVAELTD 406 Query: 241 QFPLY 245 Q+PLY Sbjct: 407 QYPLY 411 >C0Z819.1 C0Z819_BREBN Serine hydroxymethyltransferase EC=2.1.2.1 Length = 416 Score = 348 bits (893), Expect = 3e-94, Method: Compositional matrix adjust. Identities = 158/249 (63%), Positives = 198/249 (79%) Query: 1 MIVAGASAYPRTIEFEPFAQIAAEVGALFFVDMAHIAGIVAAGFHPSPIPHAHFVTTTTH 60 +IVAGASAYPRTI+F F +IA EVGA F VDMAHIAG+VAAG HP+P+PHAHFVT+TTH Sbjct: 167 LIVAGASAYPRTIDFAKFREIADEVGAYFMVDMAHIAGLVAAGLHPNPVPHAHFVTSTTH 226 Query: 61 KTLRGPRGGIIMCRKPWAQAMDKAIFPGVQGGPLMHAIAAKTVAFGEALQPEFKSYIEKV 120 KTLRGPRGG+I+C++ +A+ +DK++FPGVQGGPLMH IAAK VAFGE LQPEFK Y ++ Sbjct: 227 KTLRGPRGGLILCKEEFAKGIDKSVFPGVQGGPLMHVIAAKAVAFGENLQPEFKDYAARI 286 Query: 121 LVNAKVLAEALLNEGLTVVSGGTDNHITLLDLRPIGLNGKEAESILDEVGITANKNAIPH 180 + NA+ AE+L EGLT+VSGGTDNH+ L+D+ IGL GK AE +LDEV IT NKN IP+ Sbjct: 287 IKNARAFAESLTAEGLTLVSGGTDNHLVLIDVSKIGLTGKVAEHLLDEVSITTNKNTIPY 346 Query: 181 DTASPLITSGIRFGTPAMTTRGMGPQEMKEIARLIGLALKKPESSVAKEQLRRSVQEITS 240 DT SP +TSG+R GTPA+T+RG + MKE+A +I L LK PE + E+ R+ V + Sbjct: 347 DTQSPFVTSGVRMGTPAVTSRGFDEEAMKEVAAIIALTLKNPEDAAKHEEARQRVAALCQ 406 Query: 241 QFPLYEGLQ 249 +FP+YEGL Sbjct: 407 RFPMYEGLN 415 >B7GMG4.1 GLYA_ANOFW Serine hydroxymethyltransferase EC=2.1.2.1 Length = 413 Score = 344 bits (882), Expect = 5e-93, Method: Compositional matrix adjust. Identities = 160/245 (65%), Positives = 198/245 (80%) Query: 1 MIVAGASAYPRTIEFEPFAQIAAEVGALFFVDMAHIAGIVAAGFHPSPIPHAHFVTTTTH 60 +IVAGASAYPRTI+F F +IA EVGA F VDMAHIAG+VAAG HP+P+P+AHFVTTTTH Sbjct: 168 LIVAGASAYPRTIDFAKFREIADEVGAYFMVDMAHIAGLVAAGLHPNPVPYAHFVTTTTH 227 Query: 61 KTLRGPRGGIIMCRKPWAQAMDKAIFPGVQGGPLMHAIAAKTVAFGEALQPEFKSYIEKV 120 KTLRGPRGG+I+C++ +A+ +DKAIFPG+QGGPLMH IAAK VA GEALQ +FK+Y + + Sbjct: 228 KTLRGPRGGMILCQEQFAKQIDKAIFPGIQGGPLMHVIAAKAVALGEALQDDFKTYAQNI 287 Query: 121 LVNAKVLAEALLNEGLTVVSGGTDNHITLLDLRPIGLNGKEAESILDEVGITANKNAIPH 180 + NAK LAEAL+ EG T+VSGGTDNH+ L+DLR IGL GK AE +LDE+GIT NKN IP+ Sbjct: 288 VNNAKRLAEALVAEGFTLVSGGTDNHLLLIDLRSIGLTGKVAEKVLDEIGITVNKNTIPY 347 Query: 181 DTASPLITSGIRFGTPAMTTRGMGPQEMKEIARLIGLALKKPESSVAKEQLRRSVQEITS 240 D SP +TSGIR GT A+T+RG G +EM EIAR+I +ALK + ++ RR V +T Sbjct: 348 DPESPFVTSGIRIGTAAVTSRGFGLEEMDEIARIISIALKHKDDEQKLDEARRRVAALTE 407 Query: 241 QFPLY 245 +FPLY Sbjct: 408 KFPLY 412 >C3H9C1.1 C3H9C1_BACTU Serine hydroxymethyltransferase Length = 413 Score = 339 bits (870), Expect = 1e-91, Method: Compositional matrix adjust. Identities = 157/248 (63%), Positives = 198/248 (79%) Query: 1 MIVAGASAYPRTIEFEPFAQIAAEVGALFFVDMAHIAGIVAAGFHPSPIPHAHFVTTTTH 60 +IVAGASAYPR I+F+ F +IA EVGA VDMAHIAG+VAAG HP+P+PHAHFVTTTTH Sbjct: 166 LIVAGASAYPRVIDFKRFREIADEVGAYLMVDMAHIAGLVAAGLHPNPVPHAHFVTTTTH 225 Query: 61 KTLRGPRGGIIMCRKPWAQAMDKAIFPGVQGGPLMHAIAAKTVAFGEALQPEFKSYIEKV 120 KTLRGPRGG+I+C + +A+ +DK+IFPG+QGGPLMH IAAK VAFGEALQ +FK+Y + + Sbjct: 226 KTLRGPRGGMILCEEQFAKQIDKSIFPGIQGGPLMHVIAAKAVAFGEALQDDFKTYAQNI 285 Query: 121 LVNAKVLAEALLNEGLTVVSGGTDNHITLLDLRPIGLNGKEAESILDEVGITANKNAIPH 180 + NA LAE L EGLT+VSGGTDNH+ L+D+R + + GK AE +LDEVGIT NKN IP Sbjct: 286 INNANRLAEGLQKEGLTLVSGGTDNHLILIDVRNLEITGKVAEHVLDEVGITVNKNTIPF 345 Query: 181 DTASPLITSGIRFGTPAMTTRGMGPQEMKEIARLIGLALKKPESSVAKEQLRRSVQEITS 240 +TASP +TSG+R GT A+T+RG G +EM EIA LI LK E+ A E++R+ V+ +TS Sbjct: 346 ETASPFVTSGVRIGTAAVTSRGFGLEEMDEIASLIAYTLKNHENEAALEEVRKRVEALTS 405 Query: 241 QFPLYEGL 248 +FP+Y L Sbjct: 406 KFPMYPNL 413 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.135 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,462,614,710 Number of extensions: 61008211 Number of successful extensions: 166002 Number of sequences better than 1.0e-05: 2231 Number of HSP's gapped: 163191 Number of HSP's successfully gapped: 2232 Length of query: 250 Length of database: 3,060,696,203 Length adjustment: 132 Effective length of query: 118 Effective length of database: 1,817,122,223 Effective search space: 214420422314 Effective search space used: 214420422314 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)