BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0413.0003.p (216 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KL79.1 B3KL79_9BACI Negative regulator of genetic competence 286 7e-76 B1DJ81.1 B1DJ81_9BACL Negative regulator of genetic competence 241 5e-62 C0ZC99.1 C0ZC99_BREBN Adapter protein MecA 213 1e-53 Q5WGW2.1 Q5WGW2_BACSK Negative regulator of genetic competence 167 6e-40 C3A344.1 C3A344_BACMY Adapter protein mecA 2 162 2e-38 >B3KL79.1 B3KL79_9BACI Negative regulator of genetic competence Length = 203 Score = 286 bits (733), Expect = 7e-76, Method: Compositional matrix adjust. Identities = 139/207 (67%), Positives = 165/207 (79%), Gaps = 4/207 (1%) Query: 10 MRIERLSQDKIRIFLTFDDLSERGIQKEDMWQEVPKVHDLFTEMMDQAYSELGFDATGPL 69 M+IERLSQDKIRIFLTFDDLSERGIQKE+MWQE+PKV+DLFTEMMDQAYSELGFDATGPL Sbjct: 1 MKIERLSQDKIRIFLTFDDLSERGIQKEEMWQEIPKVYDLFTEMMDQAYSELGFDATGPL 60 Query: 70 XXXAVEVFALPAQGMVVIVTRGKYDHHQYGASGXXXXXXXXXXXXVTLEQSDSIVYAFRD 129 AVEVFALPAQGMVVIVTRGKYDHH +G VTL+QSD+IVY F+D Sbjct: 61 ---AVEVFALPAQGMVVIVTRGKYDHH-FGGLSEEDMPDEIYEMEVTLDQSDTIVYVFQD 116 Query: 130 FEALVEAAHVLIGNITSQGKLYSYNDIWYLYFDPKEFEEAALSGLVGVLSEFGDSSPVTE 189 FE L+EA+H+L + GKLY Y D W+LYF+P+E + L ++ +L+EFG+SS VTE Sbjct: 117 FEVLIEASHMLKDLVGGAGKLYHYMDKWFLYFEPEELDSEKLPAIIAMLAEFGESSSVTE 176 Query: 190 AMLAEYGKAVMPENAIELICTHFKRQQ 216 A+L EYGK VMP+ A+E+ICTHF+RQ Sbjct: 177 AVLEEYGKKVMPQQAVEVICTHFQRQH 203 >B1DJ81.1 B1DJ81_9BACL Negative regulator of genetic competence Length = 204 Score = 241 bits (614), Expect = 5e-62, Method: Compositional matrix adjust. Identities = 120/205 (58%), Positives = 149/205 (72%), Gaps = 5/205 (2%) Query: 10 MRIERLSQDKIRIFLTFDDLSERGIQKEDMWQEVPKVHDLFTEMMDQAYSELGFDATGPL 69 M+IERLSQDKIRIFLTFDDL ERGIQKEDMW+E+PKVHDLF+EMM+QAYSELGFDA+GPL Sbjct: 1 MKIERLSQDKIRIFLTFDDLLERGIQKEDMWREIPKVHDLFSEMMEQAYSELGFDASGPL 60 Query: 70 XXXAVEVFALPAQGMVVIVTRGKYD-HHQYGASGXXXXXXXXXXXXVTLEQSDSIVYAFR 128 AVEVFALPAQGMVVIVTRGK + H A+ VT+EQSD ++Y+FR Sbjct: 61 ---AVEVFALPAQGMVVIVTRGKMNGHSDERATDDEDTEDEIYEMEVTMEQSDIVMYSFR 117 Query: 129 DFEALVEAAHVL-IGNITSQGKLYSYNDIWYLYFDPKEFEEAALSGLVGVLSEFGDSSPV 187 DFE ++ L ++T G+LYSYN W L +P E A + ++ +L+E+G+++ V Sbjct: 118 DFEDVINVCKQLQAASLTEDGRLYSYNGKWILALEPALIETARHNAVIALLAEYGEATSV 177 Query: 188 TEAMLAEYGKAVMPENAIELICTHF 212 T AML EYGK +MP A+ ICTHF Sbjct: 178 TYAMLEEYGKVIMPSQAVREICTHF 202 >C0ZC99.1 C0ZC99_BREBN Adapter protein MecA Length = 199 Score = 213 bits (542), Expect = 1e-53, Method: Compositional matrix adjust. Identities = 106/203 (52%), Positives = 143/203 (70%), Gaps = 5/203 (2%) Query: 10 MRIERLSQDKIRIFLTFDDLSERGIQKEDMWQEVPKVHDLFTEMMDQAYSELGFDATGPL 69 MR+ERL QDKIRIFLTFDDLSERGI+KEDMW+++PKVH+LF +MM+QAY ELGF+ +GP+ Sbjct: 1 MRVERLGQDKIRIFLTFDDLSERGIEKEDMWRDIPKVHELFNDMMEQAYHELGFEVSGPV 60 Query: 70 XXXAVEVFALPAQGMVVIVTRGKYDHHQYGASGXXXXXXXXXXXXVTLEQSDSIVYAFRD 129 AVEVFALPAQGMVVIVTRGK VTLE+SD I+YAFRD Sbjct: 61 ---AVEVFALPAQGMVVIVTRGKTGSKD--GKEEEFEDEDVYELEVTLEESDLIMYAFRD 115 Query: 130 FEALVEAAHVLIGNITSQGKLYSYNDIWYLYFDPKEFEEAALSGLVGVLSEFGDSSPVTE 189 FE +VEAAH + +T+ G Y Y ++L + + ++ L+ +LSE+G+++P+T Sbjct: 116 FEHMVEAAHRINALLTNGGAAYFYQGKYHLVLEEVDLDQERYHKLIAILSEYGEATPITI 175 Query: 190 AMLAEYGKAVMPENAIELICTHF 212 +L EYGK ++ ++A++ IC HF Sbjct: 176 YVLEEYGKVIVADDAVKEICRHF 198 >Q5WGW2.1 Q5WGW2_BACSK Negative regulator of genetic competence Length = 200 Score = 167 bits (423), Expect = 6e-40, Method: Compositional matrix adjust. Identities = 91/201 (45%), Positives = 124/201 (61%), Gaps = 15/201 (7%) Query: 10 MRIERLSQDKIRIFLTFDDLSERGIQKEDMWQEVPKVHDLFTEMMDQAYSELGFDATGPL 69 MR+ERL+ DK ++FLT+DD+ ERGI KED+WQ+VPKVHDLF +MM +A ELGF GP+ Sbjct: 1 MRLERLAIDKFKVFLTYDDMDERGITKEDLWQDVPKVHDLFRDMMLEADDELGFKIDGPI 60 Query: 70 XXXAVEVFALPAQGMVVIVTRG----KYDHHQYGASGXXXXXXXXXXXXVTLEQSDSIVY 125 AVEVFA+PAQGMV I+T+G +YD Y +TL+++D + Y Sbjct: 61 ---AVEVFAMPAQGMVFIITKGDPADEYDAEDYEEG--------FIEMQLTLDETDEVFY 109 Query: 126 AFRDFEALVEAAHVLIGNITSQGKLYSYNDIWYLYFDPKEFEEAALSGLVGVLSEFGDSS 185 F FE ++ A L G+L+SY +YL F+ E EE + L+ +LSEFG + Sbjct: 110 EFSSFEDVIGLASRLHAIDMLGGELHSYQTRFYLKFNEAELEEVNEAALIAILSEFGSPA 169 Query: 186 PVTEAMLAEYGKAVMPENAIE 206 + + EYGKA+MP AIE Sbjct: 170 TTSFHRICEYGKALMPAQAIE 190 >C3A344.1 C3A344_BACMY Adapter protein mecA 2 Length = 202 Score = 162 bits (410), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 86/207 (41%), Positives = 130/207 (62%), Gaps = 8/207 (3%) Query: 10 MRIERLSQDKIRIFLTFDDLSERGIQKEDMWQEVPKVHDLFTEMMDQAYSELGFDATGPL 69 MR+ERL+ +KI+IFLTFDDLSERG+ KED+W+ PKV LF +MM +A +ELGF+A GP+ Sbjct: 1 MRLERLNYNKIKIFLTFDDLSERGLTKEDLWRNAPKVQQLFRDMMQEANTELGFEADGPI 60 Query: 70 XXXAVEVFALPAQGMVVIVTRGKYDHHQYGASGXXXXXXXXXXXXVTLEQSDSIVYAFRD 129 AVEVF+L AQGMVVIVT+ Y+ VTL++S+ I+Y F Sbjct: 61 ---AVEVFSLQAQGMVVIVTKENYE-----VDTEDEFRDEFIEMQVTLDESEHILYEFAT 112 Query: 130 FEALVEAAHVLIGNITSQGKLYSYNDIWYLYFDPKEFEEAALSGLVGVLSEFGDSSPVTE 189 + ++ ++ L + GKLY+++D +YL+ + +E + + + +L+E+G+ S T Sbjct: 113 LDDVINLSNRLYNLGVTDGKLYTWDDRFYLWIEEEEQFQLLKADFIAILAEYGNPSTATI 172 Query: 190 AMLAEYGKAVMPENAIELICTHFKRQQ 216 + EYGK +M AIE I +F ++Q Sbjct: 173 YRVMEYGKELMDSQAIEQIYNYFVKKQ 199 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.136 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,134,306,741 Number of extensions: 40804675 Number of successful extensions: 90710 Number of sequences better than 1.0e-05: 228 Number of HSP's gapped: 90527 Number of HSP's successfully gapped: 276 Length of query: 216 Length of database: 3,060,696,203 Length adjustment: 130 Effective length of query: 86 Effective length of database: 1,835,964,253 Effective search space: 157892925758 Effective search space used: 157892925758 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)