BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0413.0001.p (322 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KL77.1 B3KL77_9BACI Polysaccharide deacetylase 306 2e-81 B1DJ83.1 B1DJ83_9BACL Polysaccharide deacetylase 254 6e-66 Q5WFB6.1 Q5WFB6_BACSK Polysaccharide deacetylase 136 3e-30 Q2B4X9.1 Q2B4X9_9BACI Polysaccharide deacetylase 133 2e-29 A8U8J6.1 A8U8J6_9LACT Polysaccharide deacetylase 130 2e-28 >B3KL77.1 B3KL77_9BACI Polysaccharide deacetylase Length = 308 Score = 306 bits (784), Expect = 2e-81, Method: Compositional matrix adjust. Identities = 154/221 (69%), Positives = 172/221 (77%), Gaps = 4/221 (1%) Query: 104 PAAEVPL--LYHMNKNYDIVPNAEGTNKKVVLLTFDDGPKEADMINSLIDTLDKHHAKAI 161 P AE P YHMNK Y IVPN E ++KVVLLTFDDGPK+ MIN +IDTLDKH AKAI Sbjct: 90 PEAEAPSEPKYHMNKVYRIVPNDESVHEKVVLLTFDDGPKDEKMINRMIDTLDKHEAKAI 149 Query: 162 FFVNGYRVKEHPELLKLIKDRGGIIGNHSWDHIVLKDKPYAEVKKQIEDVQKIVKEITGE 221 FFVNGYR KEHPELLKLI DRG IIGNHSWDHI LK + +V+KQI DVQKIVKE TG Sbjct: 150 FFVNGYRAKEHPELLKLIHDRGQIIGNHSWDHIDLKKESKQKVEKQIGDVQKIVKEATGA 209 Query: 222 APHFFRPPHGAGGDVGKKIAAENGLLYMTWSVGSLDWEMKEKDTNKTAKLITNVTDQLHS 281 P FFRPP G+GGD K++A E+ LL+MTWS GSLDWE K K NK +++NV +QLH Sbjct: 210 EPKFFRPPFGSGGDTVKEVALEHDLLFMTWSNGSLDWESKNK--NKPDAVVSNVLEQLHP 267 Query: 282 GSNILMHELPWTVEALDTLLTTLEGKGYSFADPRSIELKMR 322 GSNILMHELPWTVEALDTLLT LE KGY F DP+ IE K+R Sbjct: 268 GSNILMHELPWTVEALDTLLTKLEAKGYGFVDPQWIEPKIR 308 >B1DJ83.1 B1DJ83_9BACL Polysaccharide deacetylase Length = 288 Score = 254 bits (650), Expect = 6e-66, Method: Compositional matrix adjust. Identities = 134/229 (58%), Positives = 161/229 (70%), Gaps = 5/229 (2%) Query: 92 SAVSGEQPAGDTPAAEVPLLYHMNKNYDIVPNAEG-TNKKVVLLTFDDGPKEADMINSLI 150 SA +P+ +P + PL Y M Y +VP E T+KK VLLTFDD P ++INSL+ Sbjct: 62 SATPAAEPS-PSPKVKAPL-YKMTTVYRLVPIDETKTSKKAVLLTFDDAPHNEEVINSLL 119 Query: 151 DTLDKHHAKAIFFVNGYRVKEHPELLKLIKDRGGIIGNHSWDHIVLKDKPYAEVKKQIED 210 DTLDKH AKAIFFVNGYRVK+HPELLKLI DRG IGNHSWDHI LK + +V+ QI D Sbjct: 120 DTLDKHKAKAIFFVNGYRVKQHPELLKLINDRGQTIGNHSWDHIDLKKEEPGKVESQIAD 179 Query: 211 VQKIVKEITGEAPHFFRPPHGAGGDVGKKIAAENGLLYMTWSVGSLDWEMKEKDTNKTAK 270 VQ VK + G+ P FFRPP+G+GGD K A +G+L+MTWS GSLDWE +K K Sbjct: 180 VQAEVKALIGKEPIFFRPPYGSGGDTVKAAAKNHGMLFMTWSNGSLDWE--QKAPGDPDK 237 Query: 271 LITNVTDQLHSGSNILMHELPWTVEALDTLLTTLEGKGYSFADPRSIEL 319 +I NV +QLH G+NILMHEL WT ALD LLT L KGYSF DP +I++ Sbjct: 238 VIGNVLEQLHPGANILMHELKWTAAALDGLLTKLGEKGYSFIDPETIKV 286 >Q5WFB6.1 Q5WFB6_BACSK Polysaccharide deacetylase Length = 275 Score = 136 bits (342), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 79/211 (37%), Positives = 119/211 (56%), Gaps = 8/211 (3%) Query: 111 LYHMNKNYDIVPNAEGT--NKKVVLLTFDDGPKEADMINSLIDTLDKHHAKAIFFVNGYR 168 LY +N N V EGT N+KVVLLT DD P + + TL++ + AIFFVNG+ Sbjct: 68 LYEINSNSWTVEPIEGTDANEKVVLLTIDDAPDAYGL--EMAQTLEELNVPAIFFVNGHF 125 Query: 169 V--KEHPELLKLIKDRGGIIGNHSWDHIVLKDKPYAEVKKQIEDVQKIVKEITGEAPHFF 226 + +E + LK I D G IGNH+ H L D +++I ++ ++++ GE P FF Sbjct: 126 IGDEEGADELKQIHDMGFAIGNHTMSHQKLDDVGEEVQQQEIVELNDRIEDVIGERPRFF 185 Query: 227 RPPHGAGGDVGKKIAAENGLLYMTWSVGSLDWEMKEKDTNKTAKLITNVTDQLHSGSNIL 286 R PHG + + + E G+L M W+ G DWE + + A ++ + T+ L +G+N+L Sbjct: 186 RAPHGINTEYSRSLVEEEGMLNMNWTYG-YDWESEYQSEEAIADIMVH-TELLTNGANLL 243 Query: 287 MHELPWTVEALDTLLTTLEGKGYSFADPRSI 317 MH+ WT AL+ ++T L KGY F DP + Sbjct: 244 MHDREWTAGALEEIVTGLRDKGYDFVDPAQL 274 >Q2B4X9.1 Q2B4X9_9BACI Polysaccharide deacetylase Length = 289 Score = 133 bits (335), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 89/228 (39%), Positives = 128/228 (56%), Gaps = 11/228 (4%) Query: 97 EQPAGDTPAAEVPLLYHMNKN-YDIVPNAEGTNKKVVLLTFDDGPKEADMINSLIDTLDK 155 EQPA +T E LY +N+N + I P A+ NKKVVLLT DD P + + + TL + Sbjct: 70 EQPA-ETEGKEP--LYRINENNWTIEPLADA-NKKVVLLTIDDAPDKHAV--EMAKTLHQ 123 Query: 156 HHAKAIFFVNGYRVK--EHPELLKLIKDRGGIIGNHSWDHIVLKDKPYAEVKKQIEDVQK 213 AIFFVNG+ + E E LK I + G IGNH++ H LK+ A+ K+++ + K Sbjct: 124 LGVSAIFFVNGHFLDTPEEEEKLKEIHNLGFPIGNHTYSHSNLKEITPAQQKEEVLKLNK 183 Query: 214 IVKEITGEAPHFFRPPHGAGGDVGKKIAAENGLLYMTWSVGSLDWEMKEKDTNKTAKLIT 273 V+EI GE P FFR P G D ++ AA G+ M W+ G DWE ++ ++ Sbjct: 184 RVEEIIGEKPRFFRAPFGINTDASREAAAAEGMELMNWTYG-YDWEKDYQNKEALTDIMV 242 Query: 274 NVTDQLHSGSNILMHELPWTVEALDTLLTTLEGKGYSFADPRSIELKM 321 N + L G+N+LMH+ WT AL+ ++ L+ KGY ADP I+ + Sbjct: 243 N-SPYLTDGANLLMHDRDWTNAALEGIVKGLQAKGYEIADPDRIQTAL 289 >A8U8J6.1 A8U8J6_9LACT Polysaccharide deacetylase Length = 282 Score = 130 bits (326), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 83/222 (37%), Positives = 116/222 (52%), Gaps = 7/222 (3%) Query: 104 PAAEVPLLYHMNKNYDIV-PNAEGTNKKVVLLTFDDGPKEADMINSLIDTLDKHHAKAIF 162 P + Y +N +V P TN KV LLTFDD P + + + L +A AIF Sbjct: 63 PTEQEAYRYKINPTTSVVEPIDSETNPKVALLTFDDSPDQHAV--DIAKQLKTINAPAIF 120 Query: 163 FVNGYRVK--EHPELLKLIKDRGGIIGNHSWDHIVLKDKPYAEVKKQIEDVQKIVKEITG 220 FVNG ++ E + LK I D G IGNH+ H L E +++I +++ E+TG Sbjct: 121 FVNGMYIESDEGKDKLKQIYDMGFEIGNHTQTHPNLSTISSEEQREEILKTNELIYEVTG 180 Query: 221 EAPHFFRPPHGAGGDVGKKIAAENGLLYMTWSVGSLDWEMKEKDTNKTAKLITNVTDQLH 280 E PHFFR PHG + I AE G++ M W+ G DWE + T++ I T+ L+ Sbjct: 181 EKPHFFRAPHGVTTETSDAIMAEEGMVSMNWTYG-YDWE-ADYQTSEALTDIMLTTEYLN 238 Query: 281 SGSNILMHELPWTVEALDTLLTTLEGKGYSFADPRSIELKMR 322 G+N+LMH+ WT EA+ + L KGY DP+ IE R Sbjct: 239 DGANLLMHDRSWTNEAVLPIAEGLVEKGYILVDPKLIETPER 280 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.314 0.134 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,596,824,930 Number of extensions: 68406285 Number of successful extensions: 136073 Number of sequences better than 1.0e-05: 2221 Number of HSP's gapped: 133186 Number of HSP's successfully gapped: 2223 Length of query: 322 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 186 Effective length of database: 1,779,438,163 Effective search space: 330975498318 Effective search space used: 330975498318 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 131 (55.1 bits)