BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0412.0015.p (449 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DHC6.1 B1DHC6_9BACL Beta-galactosidase EC=3.2.1.23 618 e-175 C4D8M8.1 C4D8M8_9SPHI Beta-galactosidase 577 e-163 B7B1R8.1 B7B1R8_9FIRM Putative uncharacterized protein 471 e-131 A6THC9.1 A6THC9_KLEP7 Putative glycoside hydrolase, family 42 464 e-129 B5Y2Z1.1 B5Y2Z1_KLEP3 Glycosyl hydrolase, family 42 464 e-128 >B1DHC6.1 B1DHC6_9BACL Beta-galactosidase EC=3.2.1.23 Length = 668 Score = 618 bits (1593), Expect = e-175, Method: Compositional matrix adjust. Identities = 293/442 (66%), Positives = 337/442 (76%), Gaps = 2/442 (0%) Query: 1 VNEYKQPGQFVTQNFDFEWRGHSFGVQPSVDHFAAAQAFDIAGVDIYHPSQHELTGTEIA 60 VNEYK+PGQFVT NFDFEWRG+SFGVQP VDHF AA+A DIAGVDIYHPSQ +LTG EI+ Sbjct: 226 VNEYKRPGQFVTHNFDFEWRGYSFGVQPDVDHFEAAKALDIAGVDIYHPSQDDLTGKEIS 285 Query: 61 FGGDIARSLKRSNYLVLETQAQAFPHWTPYPGQLRLQAFSHLASGANMVAYWHWHSIHNS 120 FGGD RSLK NY VLETQAQAFP+WTPYPGQL QAFSHLASGANMV YWHWHSIHNS Sbjct: 286 FGGDSTRSLKGMNYFVLETQAQAFPNWTPYPGQLLQQAFSHLASGANMVEYWHWHSIHNS 345 Query: 121 IETYWKGLLSHDFQPNPVYNEAVTIGRDFARLSGSLYGLRKKNKAAVMISNEALTALEWF 180 ETYWKGLLSHD PNP YNEA +GR FARLS L L+K N+AAV++SNEALT+L+WF Sbjct: 346 FETYWKGLLSHDLGPNPTYNEARIVGRQFARLSDKLVNLKKTNRAAVLVSNEALTSLDWF 405 Query: 181 KLPDGK-NYNDVVRWLYDGLYRMNIECDFIQPGD-EGLSGYSLIIVPALYAVSDEALERL 238 KLP G+ YND+VR +YD LY MNI CD + PG E L Y LI+VPALYA S E LE L Sbjct: 406 KLPGGEVRYNDIVRLMYDRLYEMNIGCDILHPGSIELLGNYDLIVVPALYAASGELLEAL 465 Query: 239 NAYVKQGGHAVYTFKSGFADETVKVRTAVQPGIISEACGISYSLFVTPHEVRLKSERAAI 298 N YV+ GGH VYTFKSGFA+E +KVRT QPG+I +ACGI YS F P +V LK + Sbjct: 466 NRYVENGGHVVYTFKSGFANEHIKVRTGAQPGLIEKACGIRYSQFAEPSKVSLKGNPFGV 525 Query: 299 SAEDNKVESWMELITPATAEVLLRYEHPHWGEYAAVTRNRYGQGSATYIGCMTSREMAAS 358 DN VE WMEL+TP TAEVL Y+HPHWG+YAA+T NR+G+G ATYIGC+ S A Sbjct: 526 GESDNNVEVWMELLTPTTAEVLAWYDHPHWGQYAAITTNRHGEGVATYIGCLPSEAAARK 585 Query: 359 ILKAAVQEAGLWGPDQELAFPLIVKTGVNPQNRKVRYYFNYSDSLQSLVYPHGPAKELLS 418 +L+ A +EAG+WG DQEL FP+IVKTG N + +RYYFNYSD QS VYPHG ELL Sbjct: 586 VLEGACREAGIWGVDQELQFPVIVKTGTNESGKTIRYYFNYSDEPQSFVYPHGDGAELLG 645 Query: 419 GRTVAPKDGLELSAWGLVILEE 440 + + +EL WG+ I+EE Sbjct: 646 DLGLRSGETIELERWGVAIVEE 667 >C4D8M8.1 C4D8M8_9SPHI Beta-galactosidase Length = 701 Score = 577 bits (1487), Expect = e-163, Method: Compositional matrix adjust. Identities = 274/447 (61%), Positives = 328/447 (73%), Gaps = 8/447 (1%) Query: 1 VNEYKQPGQFVTQNFDFEWRGHSFGVQPSVDHFAAAQAFDIAGVDIYHPSQHELTGTEIA 60 VNEYK+PGQFVTQNFD EWRG+SFG+QP VDHFAAA+ DIAG+DIYHP+Q+ LTG EIA Sbjct: 253 VNEYKRPGQFVTQNFDLEWRGYSFGIQPDVDHFAAAKPLDIAGIDIYHPTQNALTGIEIA 312 Query: 61 FGGDIARSLKRS------NYLVLETQAQAFPHWTPYPGQLRLQAFSHLASGANMVAYWHW 114 GGD+ARS+K NYLVLET+AQ F W PYPGQLRLQAFSHLASGANMV+YW W Sbjct: 313 LGGDLARSMKVDGSNRGRNYLVLETEAQGFAEWLPYPGQLRLQAFSHLASGANMVSYWPW 372 Query: 115 HSIHNSIETYWKGLLSHDFQPNPVYNEAVTIGRDFARLSGSLYGLRKKNKAAVMISNEAL 174 HS+HN+IETYWKGLLSHDFQPNP ++EA TIG+DF RLS L L+K N+ AV+ SNEAL Sbjct: 373 HSVHNAIETYWKGLLSHDFQPNPTFDEAKTIGKDFERLSPQLVNLKKTNQVAVLFSNEAL 432 Query: 175 TALEWFKLP--DGKNYNDVVRWLYDGLYRMNIECDFIQPGDEGLSGYSLIIVPALYAVSD 232 TA F+ + YND++R +YD LYRMN+ DF+ P L Y LI+VP LYA SD Sbjct: 433 TAFNAFRFGWYNRDTYNDILRPMYDALYRMNVGVDFVDPSSTNLQQYKLIVVPVLYAASD 492 Query: 233 EALERLNAYVKQGGHAVYTFKSGFADETVKVRTAVQPGIISEACGISYSLFVTPHEVRLK 292 E L RLN YVK GGH VYTFKSGF D+ VKVRT VQP II EA GI+YS F TP V LK Sbjct: 493 ELLHRLNNYVKNGGHIVYTFKSGFTDQNVKVRTQVQPAIIGEALGITYSQFTTPQNVTLK 552 Query: 293 SERAAISAEDNKVESWMELITPATAEVLLRYEHPHWGEYAAVTRNRYGQGSATYIGCMTS 352 + + AE NKV++WMELITP TA+V+ Y+HP WG+YAAVT+N +G+G ATYIGC T Sbjct: 553 GDPYGVGAEHNKVKTWMELITPTTAKVMAYYDHPVWGKYAAVTQNAFGKGLATYIGCWTD 612 Query: 353 REMAASILKAAVQEAGLWGPDQELAFPLIVKTGVNPQNRKVRYYFNYSDSLQSLVYPHGP 412 + IL AV++A LWG Q LAFP+I + GVN Q + ++Y FNYS S+ YP Sbjct: 613 DAITEKILADAVKKANLWGDAQSLAFPIITRQGVNQQGKTIQYVFNYSAKPVSVTYPFAN 672 Query: 413 AKELLSGRTVAPKDGLELSAWGLVILE 439 +ELL G++V LEL WG+ I+E Sbjct: 673 GRELLQGQSVLKNGKLELEPWGIKIIE 699 >B7B1R8.1 B7B1R8_9FIRM Putative uncharacterized protein Length = 685 Score = 471 bits (1213), Expect = e-131, Method: Compositional matrix adjust. Identities = 235/446 (52%), Positives = 294/446 (65%), Gaps = 8/446 (1%) Query: 1 VNEYKQPGQFVTQNFDFEWRGHSFGVQPSVDHFAAAQAFDIAGVDIYHPSQHELTGTEIA 60 V EY + GQF+TQNFD+ WRG SFG+QP VDHF A+A +AGVDIYHP+Q LTGTEIA Sbjct: 242 VREYLREGQFITQNFDYAWRGWSFGLQPQVDHFQCAEAVSLAGVDIYHPTQSALTGTEIA 301 Query: 61 FGGDIARSLKRSNYLVLETQAQAFPHWTPYPGQLRLQAFSHLASGANMVAYWHWHSIHNS 120 FGGDI+RSLK+ NYLV+ET+AQ FP WTPYPGQLRLQAFSHLASGA V YWHWHS+HNS Sbjct: 302 FGGDISRSLKQKNYLVVETEAQGFPQWTPYPGQLRLQAFSHLASGACGVMYWHWHSLHNS 361 Query: 121 IETYWKGLLSHDFQPNPVYNEAVTIGRDFARLSGSLYGLRKKNKAAVMISNEALTALEWF 180 ETYWKG+LSHD +P +Y E +GR+ ARLS L GLRKKN+ A+++SNEAL+ALE Sbjct: 362 WETYWKGVLSHDLKPGRIYREVQEVGREMARLSTHLQGLRKKNRVAMLVSNEALSALEHH 421 Query: 181 KLPD-----GKNYNDVVRWLYDGLYRMNIECDFIQPGDEGLSGYSLIIVPALYAVSDEAL 235 + D YNDV+RWLYD YR NIECDF+ P Y L++VPALY + L Sbjct: 422 PIGDVLRKRDFKYNDVMRWLYDAFYRSNIECDFLPPTTRDFQAYDLVLVPALYTAEEALL 481 Query: 236 ERLNAYVKQGGHAVYTFKSGFADETVKVRTAVQPGIISEACGISYSLFVTPHEVRLKSER 295 + L YV GGH + TFK+GF DE VKV QP ++E G+ Y F ++RL E Sbjct: 482 DALKQYVADGGHLLATFKTGFTDENVKVWHDDQPHHLTEVFGLRYQEFTDTPDMRLWGEN 541 Query: 296 AAISAEDNKVESWMELITPATAEVLLRYEHPHWGEYAAVTRNRYGQGSATYIGCMTSREM 355 + A +W EL+ P TA+VL Y++P W YAAVT N YG+G A Y+GC S Sbjct: 542 SLSGA---TCSAWEELLMPTTAKVLASYDNPAWQPYAAVTENVYGKGKAWYLGCYFSPVH 598 Query: 356 AASILKAAVQEAGLWGPDQELAFPLIVKTGVNPQNRKVRYYFNYSDSLQSLVYPHGPAKE 415 ++A + +A + FP I++TG N ++ YY NYSD+ QS+ Y AKE Sbjct: 599 LEQFVRAVIAKALPAIRPYDAVFPRIIRTGYNAAGHRLTYYLNYSDTEQSIAYAGRAAKE 658 Query: 416 LLSGRTVAPKDGLELSAWGLVILEED 441 L +GR V + L L WG+ I+EED Sbjct: 659 LTTGRRVEDGNALTLRPWGVRIVEED 684 >A6THC9.1 A6THC9_KLEP7 Putative glycoside hydrolase, family 42 Length = 669 Score = 464 bits (1194), Expect = e-129, Method: Compositional matrix adjust. Identities = 239/444 (53%), Positives = 286/444 (64%), Gaps = 6/444 (1%) Query: 1 VNEYKQPGQFVTQNFDFEWRGHSFGVQPSVDHFAAAQAFDIAGVDIYHPSQHELTGTEIA 60 V EY QP QFVT NFDFEWRG+S+GVQP VDHFAAAQA DIAGVDIYHPSQ LTG EIA Sbjct: 226 VREYAQPHQFVTHNFDFEWRGYSYGVQPRVDHFAAAQALDIAGVDIYHPSQAHLTGREIA 285 Query: 61 FGGDIARSLKR-SNYLVLETQAQAFPHWTPYPGQLRLQAFSHLASGANMVAYWHWHSIHN 119 FGG I RSLK NY VLETQAQ F WTP+PGQLRLQAFSH+ASGA MV+YWHWHSIHN Sbjct: 286 FGGAITRSLKPGQNYFVLETQAQGFAQWTPFPGQLRLQAFSHIASGAAMVSYWHWHSIHN 345 Query: 120 SIETYWKGLLSHDFQPNPVYNEAVTIGRDFARLSGSLYGLRKKNKAAVMISNEALTALEW 179 + ETYWKGLLSHDF NP + EA TIG DFARLS L L+ +N A++ISNEA+ AL Sbjct: 346 AFETYWKGLLSHDFSRNPTWQEATTIGADFARLSPQLAELKAENDVALLISNEAMDALNH 405 Query: 180 FKLPD--GKNYNDVVRWLYDGLYRMNIECDFIQPGDEGLSGYSLIIVPALYAVSDEALER 237 F D G YND+ R +D LY NI D I +E S Y ++IVP LYA + L R Sbjct: 406 FLPGDARGNIYNDIFRRFHDALYDHNISLDIIHDVNEETSRYRVLIVPGLYAADEGLLTR 465 Query: 238 LNAYVKQGGHAVYTFKSGFADETVKVRTAVQPGIISEACGISYSLFVTPHEVRLKSERAA 297 +N Y+ +GG A+ FKSGF+DE VKVR++ QPG++ ++CG+SYS F P E + S A Sbjct: 466 INDYIARGGRALIGFKSGFSDENVKVRSSAQPGVLRQSCGVSYSQFTLPEETTVSSCCAE 525 Query: 298 ISAE-DNKVESWMELITPAT-AEVLLRYEHPHWGEYAAVTRNRYGQGSATYIGCMTSREM 355 I DN+ E WMEL+TP LLRY+HP WGEYAA T YGQG A Y+G + + + Sbjct: 526 IDCRNDNQAELWMELLTPDDGTRTLLRYQHPAWGEYAAATEADYGQGRALYVGFLPQKGL 585 Query: 356 AASILKAAVQEAGLWGPDQELAFPLIVKTGVNPQNRKVRYYFNYSDSLQSLVYPHGPAKE 415 + + + L +PL+VK N + + FNYS +V Sbjct: 586 ISQLFDVLTADLTLNSRTSAYRYPLVVKKMRNRAGNNIHFLFNYSGEAMDVV-SETLGTA 644 Query: 416 LLSGRTVAPKDGLELSAWGLVILE 439 LLSG TV L L AW I+E Sbjct: 645 LLSGETVYVGQPLRLRAWEFTIIE 668 >B5Y2Z1.1 B5Y2Z1_KLEP3 Glycosyl hydrolase, family 42 Length = 669 Score = 464 bits (1193), Expect = e-128, Method: Compositional matrix adjust. Identities = 239/444 (53%), Positives = 284/444 (63%), Gaps = 6/444 (1%) Query: 1 VNEYKQPGQFVTQNFDFEWRGHSFGVQPSVDHFAAAQAFDIAGVDIYHPSQHELTGTEIA 60 V EY QP QFVT NFDFEWRG+S+GVQP VDHFAAAQA DIAGVDIYHPSQ LTG EIA Sbjct: 226 VREYAQPHQFVTHNFDFEWRGYSYGVQPRVDHFAAAQALDIAGVDIYHPSQAHLTGREIA 285 Query: 61 FGGDIARSLKR-SNYLVLETQAQAFPHWTPYPGQLRLQAFSHLASGANMVAYWHWHSIHN 119 FGG I RSLK NY VLETQAQ F WTP+PGQLRLQAFSH+ASGA MV+YWHWHSIHN Sbjct: 286 FGGAITRSLKPGQNYFVLETQAQGFAQWTPFPGQLRLQAFSHIASGAAMVSYWHWHSIHN 345 Query: 120 SIETYWKGLLSHDFQPNPVYNEAVTIGRDFARLSGSLYGLRKKNKAAVMISNEALTALEW 179 + ETYWKGLLSHDF NP + EA TIG DFARLS L L+ +N A++ISNEA+ AL Sbjct: 346 AFETYWKGLLSHDFSRNPTWQEATTIGADFARLSPQLAELKAENDVALLISNEAMDALNH 405 Query: 180 FKLPD--GKNYNDVVRWLYDGLYRMNIECDFIQPGDEGLSGYSLIIVPALYAVSDEALER 237 F+ D G YND+ R +D LY NI D I +E S Y ++IVP LYA D L R Sbjct: 406 FRPGDAEGNIYNDIFRRFHDALYDHNISLDIIHDVNEETSRYRVLIVPGLYAADDGLLTR 465 Query: 238 LNAYVKQGGHAVYTFKSGFADETVKVRTAVQPGIISEACGISYSLFVTPHEVRLKSERAA 297 +N Y+ +GG A+ FKSGF+DE VKVR + QPGI+ ++CG+SYS F P + S A Sbjct: 466 INDYIARGGRALIGFKSGFSDENVKVRNSAQPGILRQSCGVSYSQFTLPEGTTVSSCCAE 525 Query: 298 ISAE-DNKVESWMELITP-ATAEVLLRYEHPHWGEYAAVTRNRYGQGSATYIGCMTSREM 355 I DN+ E WMEL+TP LLRY+HP WGEYAA T YGQG A Y+G + + + Sbjct: 526 IDCRNDNQAELWMELLTPDGGTRTLLRYQHPAWGEYAAATAADYGQGRALYVGFLPHKRL 585 Query: 356 AASILKAAVQEAGLWGPDQELAFPLIVKTGVNPQNRKVRYYFNYSDSLQSLVYPHGPAKE 415 + + + L +PL+VK N + + FNYS +V Sbjct: 586 ISQLFDVLTADLTLNSRTSAYRYPLVVKKMRNRAGNTIHFLFNYSGEALDVV-SETAGTA 644 Query: 416 LLSGRTVAPKDGLELSAWGLVILE 439 LLSG V L L AW I+E Sbjct: 645 LLSGEAVDVGQPLRLRAWEFTIIE 668 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.134 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,774,711,437 Number of extensions: 114419876 Number of successful extensions: 252856 Number of sequences better than 1.0e-05: 359 Number of HSP's gapped: 252147 Number of HSP's successfully gapped: 360 Length of query: 449 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 310 Effective length of database: 1,751,175,118 Effective search space: 542864286580 Effective search space used: 542864286580 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)