BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0412.0006.p (313 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KAD7.1 B3KAD7_9BACI Alcohol dehydrogenase zinc-binding domain ... 389 e-106 B2IUU7.1 B2IUU7_NOSP7 Alcohol dehydrogenase, zinc-binding domain... 248 6e-64 A8ZMF3.1 A8ZMF3_ACAM1 Oxidoreductase, zinc-binding dehydrogenase... 182 3e-44 A4ZHT4.1 A4ZHT4_MYCS2 Putative oxydoreductase 180 2e-43 C2A7I3.1 C2A7I3_THECU Zn-dependent oxidoreductase, NADPH:quinone... 171 8e-41 >B3KAD7.1 B3KAD7_9BACI Alcohol dehydrogenase zinc-binding domain protein Length = 313 Score = 389 bits (999), Expect = e-106, Method: Compositional matrix adjust. Identities = 195/312 (62%), Positives = 228/312 (73%) Query: 1 MIRAIVSDPNSPSNLTIQEVAPPQAQPWESIVEVHAISLNRGEIKDAVNQRTSSRLGWDF 60 MIRAIV DP +PS+L +EV PQAQPWE++V+V +SLNRGE+KD Q S R GWDF Sbjct: 1 MIRAIVVDPLAPSHLAFKEVEAPQAQPWEALVQVKTVSLNRGEVKDVKEQEASRRPGWDF 60 Query: 61 AGVVAKSAEMGNGPKAGARVVGLLPMGAWGERIAVPSSALAEIPDNVRFAEAATLPVAGL 120 AG+V + A+ G GP+ GARVVGLLPMGAW E++A P S LA+IPD + F EAATLPVAGL Sbjct: 61 AGIVIEPAKNGAGPQKGARVVGLLPMGAWSEQVAAPVSMLAQIPDKLTFTEAATLPVAGL 120 Query: 121 TALNAXXXXXXXXXXXVLITGSTGGVGLFAHQLAKLSGAFNVGIASTEEKAELVLEAGAN 180 TAL A + ITGSTGGVGLFAHQLA SGA+ VG ASTEEKAELV EAGA Sbjct: 121 TALYALRKGGMLLGKRIFITGSTGGVGLFAHQLAAQSGAYIVGTASTEEKAELVREAGAE 180 Query: 181 EVLVGASVPSHVSQFGPYDLIIDSVGGDTXXXXXXXXXXXGSCIAVGFSSSPSSTLDMSN 240 EV++G S S QFGPYDLIIDSVGGDT G C+A+GFSSS +T+DMSN Sbjct: 181 EVIIGYSAISSAHQFGPYDLIIDSVGGDTLAALLPQLAPQGICVALGFSSSNIATIDMSN 240 Query: 241 FITNGGRTLYGFFLGEELTRHPAASDLAILTNLVSTGCLKPKIAVEAPWTEISSVAQQLL 300 +T GGRTLY FFLG+EL R AA DL++L LV+ G L P+I VE PWTEI SVA+ L+ Sbjct: 241 LVTRGGRTLYSFFLGDELNRQSAADDLSLLAGLVADGRLTPRIEVETPWTEIDSVARSLM 300 Query: 301 DRNISGKAVLHL 312 +R SGKAVLHL Sbjct: 301 ERKFSGKAVLHL 312 >B2IUU7.1 B2IUU7_NOSP7 Alcohol dehydrogenase, zinc-binding domain protein EC=1.6.5.5 Length = 315 Score = 248 bits (632), Expect = 6e-64, Method: Compositional matrix adjust. Identities = 146/311 (46%), Positives = 183/311 (58%), Gaps = 1/311 (0%) Query: 2 IRAIVSDPNSPSNLTIQEVAPPQAQPWESIVEVHAISLNRGEIKDAVNQRTSSRLGWDFA 61 IRA++ DPN P L + EV P+ P E++V V AISLNRGEIK + + GWD A Sbjct: 4 IRAVIVDPNVPGRLALSEVNAPKPAPDEALVRVAAISLNRGEIKRSTTAEAGWQPGWDLA 63 Query: 62 GVVAKSAEMGNGPKAGARVVGLLPMGAWGERIAVPSSALAEIPDNVRFAEAATLPVAGLT 121 GVV A +GP GARVVGL GAW E ++VP++ALAE+ +V FA+AATLPVAGLT Sbjct: 64 GVVEIPAADESGPPQGARVVGLRRAGAWAELVSVPTNALAELTPSVSFAQAATLPVAGLT 123 Query: 122 ALNAXXXXXXXXXXXVLITGSTGGVGLFAHQLAKLSGAFNVGIASTEEKAELVLEAGANE 181 A +A VL+TG++GGVG FA QLA+LSGA LV EAGA Sbjct: 124 AYHAVLKGGSLLGRPVLVTGASGGVGYFAIQLAQLSGAQVTAHIRQAGYETLVREAGAQS 183 Query: 182 VLVGASVPSHVSQFGPYDLIIDSVGGDTXXXXXXXXXXXGSCIAVGFSSSPSSTLDMSNF 241 V++G + S S++GPY LII+SVGG T G + G S T + + F Sbjct: 184 VVIGEDL-SPASEYGPYHLIIESVGGKTLGVALGLLAQDGKTVLYGVSGGAEVTFNAAQF 242 Query: 242 ITNGGRTLYGFFLGEELTRHPAASDLAILTNLVSTGCLKPKIAVEAPWTEISSVAQQLLD 301 G +LYG L +EL AA L L +LV G L P I +EA WT+I+ VAQQLLD Sbjct: 243 FGTGSVSLYGLRLFDELRFESAAVGLKRLLSLVEVGQLHPHIDLEASWTQIADVAQQLLD 302 Query: 302 RNISGKAVLHL 312 R GKAVLH+ Sbjct: 303 RRFPGKAVLHI 313 >A8ZMF3.1 A8ZMF3_ACAM1 Oxidoreductase, zinc-binding dehydrogenase family protein Length = 314 Score = 182 bits (462), Expect = 3e-44, Method: Compositional matrix adjust. Identities = 122/315 (38%), Positives = 166/315 (52%), Gaps = 5/315 (1%) Query: 2 IRAIVSDPNSPSNLTIQEVAPPQAQPWESIVEVHAISLNRGEIKDAVNQRTSSR-LGWDF 60 ++ I+ + + L+ E Q P +S+++V A SLNRGEI A + + R +GWDF Sbjct: 1 MQRIIMNSDQEGGLSFVETDSLQPLPHQSLIKVTAFSLNRGEINIAQSPTSGGRSIGWDF 60 Query: 61 AGVVAKSAEMGNGPKAGARVVGLLP-MGAWGERIAVPSSALAEIPDNVRFAEAATLPVAG 119 GV+ SA G G K G RVVG P M A+ E + +A IPD V +AATLPVAG Sbjct: 61 VGVIENSAADGTGLKKGTRVVGWRPEMDAFAEYVIGEHQFIAPIPDAVSDVQAATLPVAG 120 Query: 120 LTALNAXXXXXXXXXXXVLITGSTGGVGLFAHQLAKLSGAFNVGIASTEEKAELVLEAGA 179 LTAL A L+TG TGGVG FA QLAKLSGA V E+A GA Sbjct: 121 LTALAAVDQGNRLVGNSALVTGVTGGVGYFALQLAKLSGARTVAQVRRSEQATFASNIGA 180 Query: 180 NEVLVGASVPSHVSQFGPYDLIIDSVGGDTXXXXXXXXXXXGSCIAVGFSSSPSSTLDM- 238 + V+V S + GPY L++D VGG G+ ++ G S S ST+++ Sbjct: 181 DAVVVTED-GSGLEGEGPYHLVVDGVGGQLFSSLLSVTAKGGTLVSYGSSESHESTVNLY 239 Query: 239 -SNFITNGGRTLYGFFLGEELTRHPAASDLAILTNLVSTGCLKPKIAVEAPWTEISSVAQ 297 + F G R +YG L E+ + P+++ L L NLV LK + + W+E +A+ Sbjct: 240 PALFGNGGQRRIYGLTLYSEIEKEPSSAALTRLLNLVERDLLKLPETITSDWSETPHLAE 299 Query: 298 QLLDRNISGKAVLHL 312 LL R +GKAVL L Sbjct: 300 DLLARKFTGKAVLTL 314 >A4ZHT4.1 A4ZHT4_MYCS2 Putative oxydoreductase Length = 304 Score = 180 bits (456), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 121/312 (38%), Positives = 168/312 (53%), Gaps = 10/312 (3%) Query: 2 IRAIVSDPNSPSNLTIQEVAPPQAQPWESIVEVHAISLNRGEIKDAVNQRTSSRL-GWDF 60 +RAIV DP +P+NL EV P E++V+V AI+LN GE+ + R + GWD Sbjct: 1 MRAIVYDPLAPANLRFAEVDEPAVAAHEALVDVQAIALNFGELHFIDHARKPGEIPGWDT 60 Query: 61 AGVVAKSAEMGNGPKAGARVVGLLPMGAWGERIAVPSSALAEIPDNVRFAEAATLPVAGL 120 AG+VA++A G+GP GARVVGL G W +R A+ + LA +P+ V F EAA LPVAG+ Sbjct: 61 AGIVAQAAADGSGPPVGARVVGLNFGGGWAQRRAIATENLAVLPEFVEFEEAAALPVAGV 120 Query: 121 TALNAXXXXXXXXXXXVLITGSTGGVGLFAHQLAKLSGAFNVGIASTEEKAELVLEAGAN 180 TAL A VLITG++GGVG FA QLA +GA + + + + E GA Sbjct: 121 TALQALRALGPVVGRRVLITGASGGVGRFAVQLAARAGAHVIAAVGSPARGAGLEELGAA 180 Query: 181 EVLVGASVPSHVSQFGPYDLIIDSVGGDTXXXXXXXXXXXGSCIAVGFSSSPSSTLDMSN 240 EV VG S P ++D+VGG GS ++G +S+ +T++ Sbjct: 181 EVTVGLDEISE-----PVFGVLDNVGGPLLAQAFRLVGDGGSVQSIGMASNEPTTINFEE 235 Query: 241 FITNGGRTLYGFFLGEELTRHPAASDLAILTNLVSTGCLKPKIAVEAPWTEISSVAQQLL 300 G R F + R P A DL+ L L++ G L P+I + W IS+ A LL Sbjct: 236 ERRLGYRKRLEPF----VIRTPIAGDLSYLLTLLADGELDPQIGLRDSWDNISTAAAALL 291 Query: 301 DRNISGKAVLHL 312 R ++GKAVL + Sbjct: 292 GRTVAGKAVLRV 303 >C2A7I3.1 C2A7I3_THECU Zn-dependent oxidoreductase, NADPH:quinone reductase Length = 325 Score = 171 bits (433), Expect = 8e-41, Method: Compositional matrix adjust. Identities = 110/312 (35%), Positives = 166/312 (53%), Gaps = 13/312 (4%) Query: 2 IRAIVSDPNSPSNLTIQEVAPPQAQPWESIVEVHAISLNRGEIKDAVNQRTSSRLGWDFA 61 +RA++ DP++P+ L V P P + ++EV ISLNRGE+ A + + G+D A Sbjct: 3 MRALIVDPDAPTALRFGTVPEPDPAPGQLVLEVRHISLNRGEVTFAAQRPAGTVHGYDAA 62 Query: 62 GVVAKSAEMGNGPKAGARVVGLLPMGAWGERIAVPSSALAEIPDNVRFAEAATLPVAGLT 121 G+V ++A G GP GARV L GAW +R+AV ++A+AE+PD V A+AA LPVAG+T Sbjct: 63 GIVVRAAADGTGPSVGARVAA-LGTGAWAQRMAVDTTAVAEVPDGVDLADAAALPVAGVT 121 Query: 122 ALNAXXXXXXXXXXXVLITGSTGGVGLFAHQLAKLSGAFNVGIASTEEKAELVLEAGANE 181 AL A VL+TG+ GGVG +A QLA L GA + + + + + GA + Sbjct: 122 ALRA-LRTRPILGRRVLVTGAAGGVGRYAVQLAALGGAHVIASVGSAARTAGLADLGARQ 180 Query: 182 VLVGASVPSHVSQFGPYDLIIDSVGGDTXXXXXXXXXXXGSCIAVGFSSSPSSTLDMSNF 241 V++G P DLI+D+VGG G ++G++S + + + Sbjct: 181 VVIGLDGIDR-----PVDLILDTVGGPQLAAAWRLLAPGGDVQSIGWASGEPAVFEPYSL 235 Query: 242 ITNG-GRTLYGFFLGEELTRHPAASDLAILTNLVSTGCLKPKIAVEAPWTEISSVAQQLL 300 + G +T+ F +E DLA+L V+ G L P+I W A+ LL Sbjct: 236 YSPGPAKTISTFGDADE-----PGPDLAVLLGFVADGGLSPEIGWRGSWERFDEAARALL 290 Query: 301 DRNISGKAVLHL 312 +R ++GKAVL + Sbjct: 291 ERRVAGKAVLDV 302 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.314 0.131 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,661,341,848 Number of extensions: 62786544 Number of successful extensions: 148375 Number of sequences better than 1.0e-05: 4234 Number of HSP's gapped: 145722 Number of HSP's successfully gapped: 4247 Length of query: 313 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 178 Effective length of database: 1,788,859,178 Effective search space: 318416933684 Effective search space used: 318416933684 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 131 (55.1 bits)