BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0407.0012.p (294 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A7Z8Z1.1 A7Z8Z1_BACA2 YvbV 280 1e-73 O32256.1 YVBV_BACSU Uncharacterized transporter yvbV 273 1e-71 B1D7Y7.1 B1D7Y7_9BACL Putative uncharacterized protein 273 2e-71 B9IXC4.1 B9IXC4_BACCQ Transporter, drug/metabolite exporter family 266 1e-69 Q739Y8.1 Q739Y8_BACC1 Transporter, EamA family 266 2e-69 >A7Z8Z1.1 A7Z8Z1_BACA2 YvbV Length = 305 Score = 280 bits (716), Expect = 1e-73, Method: Compositional matrix adjust. Identities = 140/268 (52%), Positives = 180/268 (67%), Gaps = 2/268 (0%) Query: 1 MQQQRK--TIALLIFLVIVWGINWPLSKIALHYAPPLLFAGIRTXXXXXXXXXXXXPKLK 58 M+Q K T +L FLV VWGINWPL+K AL Y+PPLLFAGIRT P+ K Sbjct: 1 MKQLSKIQTALMLAFLVTVWGINWPLTKAALAYSPPLLFAGIRTLIGGLLLLMVALPRFK 60 Query: 59 ELQFRRLWPVYLVSAFLSIVFYYGFQTIGLQYVPSGLFSAIVFLQPVLLGIFAWLWLGES 118 +L+F+ WP+YL+SA L+I +YG QT+GL Y+P+GLFSAIVF QPVL+G+F+WLWLGES Sbjct: 61 KLRFKETWPIYLISAILNITLFYGLQTVGLNYLPAGLFSAIVFFQPVLMGVFSWLWLGES 120 Query: 119 MYXXXXXXXXXXXXXXXXXXIGGFTGSISVPGIVXXXXXXXXWAFATVYTKRNAARVDML 178 MY GF G IS+ GI+ WA TVY KR R D + Sbjct: 121 MYPLKIAGLILGFAGVAVISAAGFGGHISIAGILLALGSAVSWALGTVYVKRTGGRADAI 180 Query: 179 WMTAMQIMIGGLVLLAAGSATESWSDITWNAAFIVNTLFISVFVIALGWMVYFSLIKEGE 238 WM A+Q++IGG++L+++G +ES+S I W A FI++ LFISVFVIALGW+ +F+L+ GE Sbjct: 181 WMVALQLLIGGVLLVSSGHFSESFSAIQWKAPFILSLLFISVFVIALGWLAFFTLVGSGE 240 Query: 239 AGKVGSFTFLIPLISIGSSVVLLHERIT 266 A KV S+TFLIPLISI S + LHE +T Sbjct: 241 ASKVASYTFLIPLISITVSSIFLHEPLT 268 >O32256.1 YVBV_BACSU Uncharacterized transporter yvbV Length = 305 Score = 273 bits (698), Expect = 1e-71, Method: Compositional matrix adjust. Identities = 140/265 (52%), Positives = 178/265 (67%), Gaps = 2/265 (0%) Query: 1 MQQ--QRKTIALLIFLVIVWGINWPLSKIALHYAPPLLFAGIRTXXXXXXXXXXXXPKLK 58 M+Q + +T LL FLVI+WG+NWPLSK AL Y+PPLLFAGIRT P++ Sbjct: 1 MKQLPKTRTALLLAFLVIMWGVNWPLSKAALAYSPPLLFAGIRTLIGGLLLVIVALPRIH 60 Query: 59 ELQFRRLWPVYLVSAFLSIVFYYGFQTIGLQYVPSGLFSAIVFLQPVLLGIFAWLWLGES 118 +L+ + WP+YLVSA L+I +YG QTIGL Y+P+GLFSAIVF QPVL+G+F+WLWLGES Sbjct: 61 KLRLKETWPIYLVSALLNITLFYGLQTIGLNYLPAGLFSAIVFFQPVLMGVFSWLWLGES 120 Query: 119 MYXXXXXXXXXXXXXXXXXXIGGFTGSISVPGIVXXXXXXXXWAFATVYTKRNAARVDML 178 M+ GF G ISV G++ WA TVY K+ +RVD + Sbjct: 121 MFVMKVIGLILGFAGVAVISAAGFGGHISVIGVLLALGSAVSWALGTVYMKKTGSRVDSI 180 Query: 179 WMTAMQIMIGGLVLLAAGSATESWSDITWNAAFIVNTLFISVFVIALGWMVYFSLIKEGE 238 WM A+Q+ IG + LL +G TES+S I W A FI + LFISVFVIALGW+V+F+L+ GE Sbjct: 181 WMVALQLTIGSVFLLISGFWTESFSAIQWTAPFITSLLFISVFVIALGWLVFFTLVGSGE 240 Query: 239 AGKVGSFTFLIPLISIGSSVVLLHE 263 A KV S+TFLIPLISI +S + LHE Sbjct: 241 ASKVASYTFLIPLISIVASSIFLHE 265 >B1D7Y7.1 B1D7Y7_9BACL Putative uncharacterized protein Length = 298 Score = 273 bits (697), Expect = 2e-71, Method: Compositional matrix adjust. Identities = 134/266 (50%), Positives = 180/266 (67%), Gaps = 2/266 (0%) Query: 3 QQRKTIALLIFLVIVWGINWPLSKIALHYAPPLLFAGIRTXXXXXXXXXXXXPKLKELQF 62 +R+T+ L+ FLV+VWGINWPLSK ALHY PP+LF+G+RT P+ + LQF Sbjct: 5 SKRQTVLLITFLVLVWGINWPLSKYALHYMPPILFSGVRTLLGGVLLLAAAVPRYRRLQF 64 Query: 63 RRLWPVYLVSAFLSIVFYYGFQTIGLQYVPSGLFSAIVFLQPVLLGIFAWLWLGESMYXX 122 R+ WP+YL+SA +++V YYG QTIG+QY+P+GLFSAIVFLQPVL+G+F+WLWLGE M Sbjct: 65 RKTWPIYLLSAVVNVVLYYGLQTIGIQYLPAGLFSAIVFLQPVLVGLFSWLWLGELMNGL 124 Query: 123 XXXXXXXXXXXXXXXXIG--GFTGSISVPGIVXXXXXXXXWAFATVYTKRNAARVDMLWM 180 G G TG ISV GI+ WA T+Y K+++ +VD +W+ Sbjct: 125 KVIGLVLGFAGVGVISSGASGATGHISVAGILLALGSALSWALGTIYVKKSSGKVDNIWL 184 Query: 181 TAMQIMIGGLVLLAAGSATESWSDITWNAAFIVNTLFISVFVIALGWMVYFSLIKEGEAG 240 MQ++IGG+ + GS TESWS++ W+ FI + LFIS+FVIALGW+V++ L+ GEA Sbjct: 185 VTMQLIIGGVFMTGLGSVTESWSEVEWSGRFIFSLLFISIFVIALGWLVFYKLMDSGEAS 244 Query: 241 KVGSFTFLIPLISIGSSVVLLHERIT 266 KV SFTFLIPL++I + LHE T Sbjct: 245 KVASFTFLIPLVAILIGTMFLHEPFT 270 >B9IXC4.1 B9IXC4_BACCQ Transporter, drug/metabolite exporter family Length = 301 Score = 266 bits (681), Expect = 1e-69, Method: Compositional matrix adjust. Identities = 134/264 (50%), Positives = 172/264 (65%) Query: 3 QQRKTIALLIFLVIVWGINWPLSKIALHYAPPLLFAGIRTXXXXXXXXXXXXPKLKELQF 62 + KT +LIFLV +WGINWPLSK ALHY PP+LFAG+RT PK KEL Sbjct: 5 SRTKTAIILIFLVFMWGINWPLSKFALHYTPPVLFAGVRTLIGGFILLLFALPKYKELHL 64 Query: 63 RRLWPVYLVSAFLSIVFYYGFQTIGLQYVPSGLFSAIVFLQPVLLGIFAWLWLGESMYXX 122 + W +Y++S+ L+I+ +YG QT+GLQY+P+GLFSAIVFLQPVLLGIF+W+WL ESMY Sbjct: 65 KETWHLYVISSLLNIIIFYGLQTVGLQYMPAGLFSAIVFLQPVLLGIFSWIWLEESMYGL 124 Query: 123 XXXXXXXXXXXXXXXXIGGFTGSISVPGIVXXXXXXXXWAFATVYTKRNAARVDMLWMTA 182 TG IS+ GI+ WA TV+ K+ RV+ +WM Sbjct: 125 KIFGLILGFIGVGVISSSSLTGHISIIGILLAIGCAIGWALGTVFIKKTGHRVNSIWMVT 184 Query: 183 MQIMIGGLVLLAAGSATESWSDITWNAAFIVNTLFISVFVIALGWMVYFSLIKEGEAGKV 242 +Q++IGGL L+ GS ESWS I W+ F+ LFIS FVIA+GW+ YF+L+ GEA KV Sbjct: 185 LQLIIGGLCLIGFGSEFESWSSIAWSMPFVSVLLFISFFVIAMGWLAYFTLVGAGEASKV 244 Query: 243 GSFTFLIPLISIGSSVVLLHERIT 266 G++TFLIPLI+I S + LHE IT Sbjct: 245 GAYTFLIPLIAIIVSSIFLHEAIT 268 >Q739Y8.1 Q739Y8_BACC1 Transporter, EamA family Length = 301 Score = 266 bits (680), Expect = 2e-69, Method: Compositional matrix adjust. Identities = 135/264 (51%), Positives = 171/264 (64%) Query: 3 QQRKTIALLIFLVIVWGINWPLSKIALHYAPPLLFAGIRTXXXXXXXXXXXXPKLKELQF 62 + KT +L FLV +WGINWPLSK ALHY PP+LFAGIRT PK KEL Sbjct: 5 SRTKTAIILTFLVFMWGINWPLSKFALHYTPPVLFAGIRTLIGGFILLLFALPKYKELHL 64 Query: 63 RRLWPVYLVSAFLSIVFYYGFQTIGLQYVPSGLFSAIVFLQPVLLGIFAWLWLGESMYXX 122 + W +Y++S+ L+I+ +YG QT+GLQY+P+GLFSAIVFLQPVLLGIF+W+WL ESMY Sbjct: 65 KETWHLYVISSLLNIIIFYGLQTVGLQYMPAGLFSAIVFLQPVLLGIFSWIWLEESMYGL 124 Query: 123 XXXXXXXXXXXXXXXXIGGFTGSISVPGIVXXXXXXXXWAFATVYTKRNAARVDMLWMTA 182 TG ISV GI+ WA TV+ K+ RV+ +WM Sbjct: 125 KIFGLVLGFIGVGVISSSSLTGHISVIGILLALGCAIGWALGTVFIKKTGHRVNAIWMVT 184 Query: 183 MQIMIGGLVLLAAGSATESWSDITWNAAFIVNTLFISVFVIALGWMVYFSLIKEGEAGKV 242 +Q++IGGL L+ GS ESWS I W+ F+ LFIS FVIA+GW+ YF+L+ GEA KV Sbjct: 185 IQLLIGGLCLVGFGSEFESWSSIAWSMPFVSVLLFISFFVIAMGWLAYFTLVGAGEASKV 244 Query: 243 GSFTFLIPLISIGSSVVLLHERIT 266 G++TFLIPLI+I S + LHE IT Sbjct: 245 GAYTFLIPLIAIIVSSIFLHEAIT 268 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.330 0.142 0.455 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,359,610,955 Number of extensions: 46429627 Number of successful extensions: 182885 Number of sequences better than 1.0e-05: 262 Number of HSP's gapped: 183280 Number of HSP's successfully gapped: 262 Length of query: 294 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 160 Effective length of database: 1,798,280,193 Effective search space: 287724830880 Effective search space used: 287724830880 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 130 (54.7 bits)