BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0405.0010.p (543 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KLN1.1 B3KLN1_9BACI Drug resistance transporter, EmrB/QacA sub... 593 e-167 B8I5X8.1 B8I5X8_CLOCE Drug resistance transporter, EmrB/QacA sub... 581 e-164 C0Z4L4.1 C0Z4L4_BREBN Putative multidrug resistance protein 568 e-160 A6CHD2.1 A6CHD2_9BACI Major facilitator family transporter 521 e-146 Q2B8D0.1 Q2B8D0_9BACI Major facilitator family transporter 506 e-141 >B3KLN1.1 B3KLN1_9BACI Drug resistance transporter, EmrB/QacA subfamily Length = 549 Score = 593 bits (1528), Expect = e-167, Method: Compositional matrix adjust. Identities = 298/545 (54%), Positives = 394/545 (72%), Gaps = 5/545 (0%) Query: 2 EHLTQKKKVTXXXXXXXXXXXXXXNQTIVGTAMPRIIAMLGGIEHYTWVITIYMLTSTIA 61 EHLT K+KV+ NQTI TAMPRIIA+L G+++YTW I IY+LTSTIA Sbjct: 2 EHLTLKRKVSIMIAIMAAMFFAAINQTITSTAMPRIIAILDGMDYYTWTINIYLLTSTIA 61 Query: 62 TVLVGKLSDIYGRKPFLLAGIIIFMIGAFLSGLSANIFQMIAYRGIQGVGGGILMSATVT 121 T+LVGKLSD++GRKPF+L GI+ FM+GAFL+G S +++Q I YRGIQG+G GI+ S Sbjct: 62 TILVGKLSDMFGRKPFILIGILFFMLGAFLTGTSTDVYQFIIYRGIQGIGAGIIQSTAFM 121 Query: 122 AVGDLFAPRERAKWTGIMMAMFGFSSVIGPTLGGFMIDHMDWKWIFWIFLPLGLVAFVMI 181 +VGDLF PRER KW G+M A+FGFSSV+GPTLGG+++D+++W+W+FWIFLPLG+VAF+MI Sbjct: 122 SVGDLFPPRERGKWMGLMTAVFGFSSVLGPTLGGYLVDNIEWRWLFWIFLPLGVVAFIMI 181 Query: 182 WRMFPKTTRAASESIDYPGSVFLSLSIVALLLGFSWAGTQYDWGSPQXXXXXXXXXXXXX 241 W +FPK R +SIDY GS+FL+ SIV LLL FSWAGT+Y WGSPQ Sbjct: 182 WTLFPKVERKPGQSIDYLGSLFLTTSIVPLLLAFSWAGTEYAWGSPQIIGLIAASVVSAL 241 Query: 242 XXXXXERKAKSPVLPLSLFKNSIISLSMVIGFLMNAGMMGAMIYLPFFVQGVEGVSPTNS 301 E KAK PVLPLSLFKNS++++S +IGF MN G+MGAMIY+ F+VQGV G+SPT + Sbjct: 242 IFILVEMKAKEPVLPLSLFKNSVVTVSNLIGFFMNFGLMGAMIYISFYVQGVLGISPTYA 301 Query: 302 GFINMPMSLMMIVLSTLVGRWISKSGKYKRYALIGMPFMVAAMLIMAFMNSIAMAVASMI 361 G++ +PMS+ M+ S + G+ I+KSGKYKRYAL+G+P M+ M IM FM++I + V S I Sbjct: 302 GYVTIPMSIFMMGTSAIAGQLIAKSGKYKRYALLGVPIMMVGMGIMVFMDNIWLTVVSTI 361 Query: 362 IFGIGLGLAMPVFTLTVQNAVAPAMLGVATATSTLFRNLGGTIGIAIMGTVMNTTLTHKL 421 IFGIGLGL MPVF+L QNAV LGVATA+S LFRNLGGTIGIA+MGTVM+ LT L Sbjct: 362 IFGIGLGLGMPVFSLATQNAVPHNELGVATASSQLFRNLGGTIGIAVMGTVMSNNLTKNL 421 Query: 422 ENAVHSGQGPDMSKLDAESARQLSAFMNPQMLLDQPKLKELHATLPEQIQPLFNQLIEML 481 + A+ S PD S +D + A+Q+ +F NPQ L+++P +++ A+LP +QPLF Q+I+ + Sbjct: 422 KEALQSTNAPDFSSVDPQLAQQMLSFANPQTLMNKPLIEQTQASLPADVQPLFAQMIDSI 481 Query: 482 RSALSDSLTVVFLSGTXXXXXXXXXXXXXKEIPLRS----SEARTEAKTGEK-GVDSKLK 536 R AL ++L++VFL+GT KEIPLR+ S ++E G+ G ++ K Sbjct: 482 RDALGNTLSMVFLTGTLVLVVALLLVFFLKEIPLRTTNQASAPKSEDSEGKSAGAGTRAK 541 Query: 537 EQAPA 541 E + A Sbjct: 542 EVSVA 546 >B8I5X8.1 B8I5X8_CLOCE Drug resistance transporter, EmrB/QacA subfamily Length = 531 Score = 581 bits (1498), Expect = e-164, Method: Compositional matrix adjust. Identities = 284/495 (57%), Positives = 377/495 (76%) Query: 2 EHLTQKKKVTXXXXXXXXXXXXXXNQTIVGTAMPRIIAMLGGIEHYTWVITIYMLTSTIA 61 LTQK+K T NQTI+ A+P++IA LGG+++Y+WVIT YMLTSTI+ Sbjct: 2 HQLTQKRKTTVMIALMATMFFAAINQTIISAALPKVIAQLGGMDYYSWVITGYMLTSTIS 61 Query: 62 TVLVGKLSDIYGRKPFLLAGIIIFMIGAFLSGLSANIFQMIAYRGIQGVGGGILMSATVT 121 TV+ GKLSDIYGRK FLL+GIIIF+IG+FL+GLSANIFQMIA+RGIQG+GGGILMS ++T Sbjct: 62 TVIFGKLSDIYGRKIFLLSGIIIFLIGSFLTGLSANIFQMIAFRGIQGIGGGILMSISLT 121 Query: 122 AVGDLFAPRERAKWTGIMMAMFGFSSVIGPTLGGFMIDHMDWKWIFWIFLPLGLVAFVMI 181 AVGDL+ P+ERAKWTG+MM++FG SS++GP LGG M+D++ WKW+FWIFLPLG++A +MI Sbjct: 122 AVGDLYPPQERAKWTGVMMSIFGISSILGPVLGGVMVDYVAWKWLFWIFLPLGVIALIMI 181 Query: 182 WRMFPKTTRAASESIDYPGSVFLSLSIVALLLGFSWAGTQYDWGSPQXXXXXXXXXXXXX 241 W +FPK + +SESIDY GS+FL+LSI +LLLGFSWAGT+Y W PQ Sbjct: 182 WSLFPKLSGNSSESIDYLGSIFLALSITSLLLGFSWAGTKYAWALPQSLGLFAATLVGIL 241 Query: 242 XXXXXERKAKSPVLPLSLFKNSIISLSMVIGFLMNAGMMGAMIYLPFFVQGVEGVSPTNS 301 ER AKSPV+PLS+FKN +++S V F+M+ GMMGAM Y+PFF+QGV+G S T S Sbjct: 242 VLILIERNAKSPVMPLSMFKNKGVTISFVASFVMSGGMMGAMSYIPFFIQGVKGHSATTS 301 Query: 302 GFINMPMSLMMIVLSTLVGRWISKSGKYKRYALIGMPFMVAAMLIMAFMNSIAMAVASMI 361 G +N+PMSLMMI+ STLVGRW+SK G Y+ +A++G+ M A+M+IMA MN+I A S+I Sbjct: 302 GLLNIPMSLMMIIFSTLVGRWMSKYGNYRLFAVLGLGIMSASMIIMANMNNIVWAAISLI 361 Query: 362 IFGIGLGLAMPVFTLTVQNAVAPAMLGVATATSTLFRNLGGTIGIAIMGTVMNTTLTHKL 421 IFG+GLG M VFT T QN+ + LGV TA+ TLFRNLGGTI IAI G++MNT++ + Sbjct: 362 IFGVGLGFGMTVFTTTAQNSAPTSQLGVVTASCTLFRNLGGTIFIAIFGSIMNTSMARNM 421 Query: 422 ENAVHSGQGPDMSKLDAESARQLSAFMNPQMLLDQPKLKELHATLPEQIQPLFNQLIEML 481 E + SG G D++KLD E+A + A NPQ+LLD KL+ + A++PEQ+Q LF+++++++ Sbjct: 422 EKVMSSGSGIDLTKLDVETAEKFKAMANPQLLLDNTKLEAIQASMPEQVQLLFSKVMDLV 481 Query: 482 RSALSDSLTVVFLSG 496 R+ + D+LT VF G Sbjct: 482 RTVMGDALTSVFYIG 496 >C0Z4L4.1 C0Z4L4_BREBN Putative multidrug resistance protein Length = 538 Score = 568 bits (1464), Expect = e-160, Method: Compositional matrix adjust. Identities = 286/536 (53%), Positives = 379/536 (70%), Gaps = 9/536 (1%) Query: 2 EHLTQKKKVTXXXXXXXXXXXXXXNQTIVGTAMPRIIAMLGGIEHYTWVITIYMLTSTIA 61 + LT+KKKVT NQTI+G AMPRII+ LGG+++Y+W ITIY+LTST+A Sbjct: 2 QQLTEKKKVTIMIAIITAMFFAAINQTIIGVAMPRIISKLGGMDYYSWAITIYLLTSTVA 61 Query: 62 TVLVGKLSDIYGRKPFLLAGIIIFMIGAFLSGLSANIFQMIAYRGIQGVGGGILMSATVT 121 +VLVGKLSDIYGRKPF+L GI +F IGA LSG S +IFQ+I YRGIQG G GI+MS T Sbjct: 62 SVLVGKLSDIYGRKPFILTGIALFSIGALLSGFSTDIFQLITYRGIQGAGAGIIMSTAFT 121 Query: 122 AVGDLFAPRERAKWTGIMMAMFGFSSVIGPTLGGFMIDHMDWKWIFWIFLPLGLVAFVMI 181 A+GDL+ PRERAKW GIM A+FG SSV+GP +GG+++DH+DW W+FWIFLP+G++AF++I Sbjct: 122 AMGDLYEPRERAKWGGIMSAVFGVSSVLGPLMGGYIVDHLDWHWVFWIFLPIGVIAFLLI 181 Query: 182 WRMFPKTTRAASESIDYPGSVFLSLSIVALLLGFSWAGT---QYDWGSPQXXXXXXXXXX 238 FPK + ES+DY GS+FL+L+IV +LL FSWAG ++ WGS Q Sbjct: 182 MIHFPKVPKQEGESVDYFGSLFLTLTIVPMLLAFSWAGNGPGKHAWGSWQIIGLFASTII 241 Query: 239 XXXXXXXXERKAKSPVLPLSLFKNSIISLSMVIGFLMNAGMMGAMIYLPFFVQGVEGVSP 298 ERK K+PVLPL LFKNSI ++S ++GF +NAGMMGA+IY+PFFVQGV+G+SP Sbjct: 242 ALIVFIMIERKVKTPVLPLGLFKNSIFTVSNLVGFFLNAGMMGAIIYVPFFVQGVKGISP 301 Query: 299 TNSGFINMPMSLMMIVLSTLVGRWISKSGKYKRYALIGMPFMVAAMLIMAFMN---SIAM 355 T +G++ MPMS+ M+ S + G+ ++K+GKYK+ AL G+ M M++M FM+ I + Sbjct: 302 TMAGYVAMPMSIAMLGTSAIAGQIMTKTGKYKKMALGGLLVMTFGMVLMYFMSPTTPIYL 361 Query: 356 AVASMIIFGIGLGLAMPVFTLTVQNAVAPAMLGVATATSTLFRNLGGTIGIAIMGTVMNT 415 V MII G+G+G+AMPVF+LTVQNAVAP LGVATATS LFRNLGGTIGIA+MGTVM+ Sbjct: 362 LVIYMIILGLGIGIAMPVFSLTVQNAVAPQQLGVATATSQLFRNLGGTIGIAVMGTVMSA 421 Query: 416 TLTHKLENAVHS-GQGPDMSKLDAESARQLSAFMNPQMLLDQPKLKELHATLPEQIQPLF 474 ++T K+ + GQ + + D A +LS F NPQ LLDQPK++ A+LP +QPLF Sbjct: 422 SMTAKMTQLSGAMGQVNNPAAADPALAEKLSLFTNPQNLLDQPKIEAALASLPPDLQPLF 481 Query: 475 NQLIEMLRSALSDSLTVVFLSGTXXXXXXXXXXXXXKEIPLRSSE--ARTEAKTGE 528 +++M+R A+S +T FL+G KEIPLRS + + E + G+ Sbjct: 482 THMLDMIREAMSYGITTTFLTGAIVAGMAVIIALFLKEIPLRSGKMGQKPEGEAGK 537 >A6CHD2.1 A6CHD2_9BACI Major facilitator family transporter Length = 539 Score = 521 bits (1343), Expect = e-146, Method: Compositional matrix adjust. Identities = 258/535 (48%), Positives = 363/535 (67%), Gaps = 7/535 (1%) Query: 1 VEHLTQKKKVTXXXXXXXXXXXXXXNQTIVGTAMPRIIAMLGGIEHYTWVITIYMLTSTI 60 +EHL +KK+ NQTIVGTA+P II+ LGGIE+Y+WV TI+MLTS++ Sbjct: 1 MEHLEPRKKILIMLAIMSAMLFAALNQTIVGTALPTIISDLGGIEYYSWVFTIFMLTSSV 60 Query: 61 ATVLVGKLSDIYGRKPFLLAGIIIFMIGAFLSGLSANIFQMIAYRGIQGVGGGILMSATV 120 +LVGKLSD+YGRKPF+L GI +F +G+FL+GLS +I ++IAYRGIQG G G++MS + Sbjct: 61 TAILVGKLSDMYGRKPFILIGIGVFTLGSFLNGLSGSIIELIAYRGIQGFGAGMIMSTSF 120 Query: 121 TAVGDLFAPRERAKWTGIMMAMFGFSSVIGPTLGGFMIDHMDWKWIFWIFLPLGLVAFVM 180 TAVGDLFAPRER +W G+M +FG +SV GPTLGG+++D+ +W W+FW+FLP GL+AF+M Sbjct: 121 TAVGDLFAPRERGRWQGLMSGIFGLASVFGPTLGGWIVDNAEWHWVFWVFLPFGLLAFIM 180 Query: 181 IWRMFPKTTRAASESIDYPGSVFLSLSIVALLLGFSWAGTQYDWGSPQXXXXXXXXXXXX 240 IW++FP + +DY GS+ LSL+I+ +LL F+W G ++W S Q Sbjct: 181 IWKLFPSQQVQEKKKVDYLGSLMLSLTIIPMLLAFTWGGNDFEWASFQIIGLFVLTGASL 240 Query: 241 XXXXXXERKAKSPVLPLSLFKNSIISLSMVIGFLMNAGMMGAMIYLPFFVQGVEGVSPTN 300 E+KA +PVLPL LFKN I +LS +IGF++ AGM GA++Y+PFF+QGV G S T Sbjct: 241 IFFIVTEKKAANPVLPLHLFKNDIFTLSNIIGFILGAGMFGAIMYMPFFIQGVIGTSATK 300 Query: 301 SGFINMPMSLMMIVLSTLVGRWISKSGKYKRYALIGMPFMVAAMLIMAFMNS---IAMAV 357 SGF+ MPM+L M+ S + G+ I+K+GKYK AL+G+ M + + ++ M+S AV Sbjct: 301 SGFVMMPMTLSMVFASAIGGQLITKTGKYKLIALLGLFIMASGLASLSMMDSNTTNTTAV 360 Query: 358 ASMIIFGIGLGLAMPVFTLTVQNAVAPAMLGVATATSTLFRNLGGTIGIAIMGTVMNTTL 417 +MII G GLGL+ PVFTLTVQNAV LGV+TA + LFR +GGT+G+A+MGTVMN ++ Sbjct: 361 INMIIVGTGLGLSFPVFTLTVQNAVQHQYLGVSTAATQLFRQIGGTVGVAVMGTVMNNSM 420 Query: 418 THKL-ENAVHSGQGPDMSKLDAESARQLSAFMNPQMLLDQPKLKELHATLPEQIQPLFNQ 476 + K+ E + D+ L E+A QL A +PQ L++ KL+E+ A+LP ++Q +F + Sbjct: 421 SSKMNEEMSKLKENTDLEALPPETAAQLQALQSPQSLMNGEKLQEIQASLPPEMQAVFGK 480 Query: 477 LIEMLRSALSDSLTVVFLSGTXXXXXXXXXXXXXKEIPLRSS---EARTEAKTGE 528 +++++R +LS SLT VFL G +EIPLR + E + E K E Sbjct: 481 MMDIVRDSLSYSLTNVFLIGAIIMSAGFLLTFFLREIPLRKTNKDEPKAEYKKAE 535 >Q2B8D0.1 Q2B8D0_9BACI Major facilitator family transporter Length = 532 Score = 506 bits (1302), Expect = e-141, Method: Compositional matrix adjust. Identities = 268/531 (50%), Positives = 358/531 (67%), Gaps = 7/531 (1%) Query: 2 EHLTQKKKVTXXXXXXXXXXXXXXNQTIVGTAMPRIIAMLGGIEHYTWVITIYMLTSTIA 61 EHL+QK+K+T NQTIVG A+P+II+ LGG+++Y+WV TIYMLTS I Sbjct: 2 EHLSQKEKITIMIAIIAAMFFAAVNQTIVGNALPKIISDLGGLDYYSWVFTIYMLTSAIT 61 Query: 62 TVLVGKLSDIYGRKPFLLAGIIIFMIGAFLSGLSANIFQMIAYRGIQGVGGGILMSATVT 121 T+LVGKLSDIYGRKPF+L GIIIF IGAFLSG S++IF +I YRGIQG G G++MS T Sbjct: 62 TILVGKLSDIYGRKPFILIGIIIFSIGAFLSGTSSDIFDLITYRGIQGFGAGMIMSTAFT 121 Query: 122 AVGDLFAPRERAKWTGIMMAMFGFSSVIGPTLGGFMIDHMDWKWIFWIFLPLGLVAFVMI 181 AVGDLFAPRER KW G M A+FG SSV GPTLGG+++D+++WKW+FW+FLPLG+VA +I Sbjct: 122 AVGDLFAPRERGKWQGAMSAVFGISSVFGPTLGGYIVDNLEWKWVFWVFLPLGIVALALI 181 Query: 182 WRMFPKTTRAASESIDYPGSVFLSLSIVALLLGFSWAGTQYDWGSPQXXXXXXXXXXXXX 241 W++FP + ES+DY G+VFL+ +IV LLGFSWAGT+YDWGS Q Sbjct: 182 WKLFPSQEKKKGESVDYVGAVFLTATIVLTLLGFSWAGTKYDWGSSQIIMLFGGAVIALI 241 Query: 242 XXXXXERKAKSPVLPLSLFKNSIISLSMVIGFLMNAGMMGAMIYLPFFVQGVEGVSPTNS 301 ERK K+P+LPL LFKN I ++S +IGF++ M G ++Y+P+F+QGV G S T+S Sbjct: 242 LFILIERKVKTPILPLYLFKNGIFTISNIIGFVIGVSMFGGVMYVPYFIQGVLGYSATHS 301 Query: 302 GFINMPMSLMMIVLSTLVGRWISKSGKYKRYALIGMPFMVAAMLIMAFMN---SIAMAVA 358 F+ M M+L ++ S L G+ ISK+GKYK A+IG+ + +++ M+ S V Sbjct: 302 SFLTMAMTLGLVFASALGGQIISKTGKYKLQAIIGLCISAVGLYLLSRMDADTSQYALVG 361 Query: 359 SMIIFGIGLGLAMPVFTLTVQNAVAPAMLGVATATSTLFRNLGGTIGIAIMGTVMNTTLT 418 +I+ G G+G+ M VFTLTVQNAV LGVATATS LFR++GGT+G+AIMGT++N + Sbjct: 362 FLILVGFGIGIGMTVFTLTVQNAVEQKFLGVATATSQLFRSVGGTVGVAIMGTLLNNRME 421 Query: 419 HKLENAVHSGQGPDMSKLDAESARQLSAFMNPQMLLDQPKLKELHATLPEQIQPLFNQLI 478 K+ + +M+ E A +L + NPQ+LLD KL ++ ++LP + LF +I Sbjct: 422 DKMTTMSQNQNTNNMAA-SPELAGKLESLKNPQLLLDHEKLAQVKSSLPAEAASLFEHII 480 Query: 479 EMLRSALSDSLTVVFLSGTXXXXXXXXXXXXXKEIPLRS---SEARTEAKT 526 MLR ALS +L+ VFL T KEIPLRS S+A AK+ Sbjct: 481 SMLREALSYALSGVFLFVTITMLVAVILTFFLKEIPLRSAADSKAPEPAKS 531 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.324 0.137 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,687,693,492 Number of extensions: 102655208 Number of successful extensions: 588379 Number of sequences better than 1.0e-05: 14164 Number of HSP's gapped: 569723 Number of HSP's successfully gapped: 14548 Length of query: 543 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 402 Effective length of database: 1,732,333,088 Effective search space: 696397901376 Effective search space used: 696397901376 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 134 (56.2 bits)