BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0402.0041.p (412 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q65HQ2.1 Q65HQ2_BACLD YqjV 456 e-126 A8FEV6.1 A8FEV6_BACP2 MFS family major facilitator transporter, ... 442 e-122 B4ALB9.1 B4ALB9_BACPU MFS family major facilitator transporter, ... 442 e-122 P54559.2 YQJV_BACSU Uncharacterized MFS-type transporter yqjV 436 e-120 Q733L7.1 Q733L7_BACC1 Conserved domain protein 381 e-104 >Q65HQ2.1 Q65HQ2_BACLD YqjV Length = 412 Score = 456 bits (1174), Expect = e-126, Method: Compositional matrix adjust. Identities = 220/401 (54%), Positives = 290/401 (72%), Gaps = 2/401 (0%) Query: 1 MKQHLQQIHPLAWTIIIGTMFGRLVTSMSIPFLSIYLTQVLGASHSQTGITVAVSSLAGV 60 MKQ Q IHPL+WTII+GT+FGR+ T+MSIPFL+IYLTQV GAS G +A SSL G+ Sbjct: 1 MKQKWQNIHPLSWTIIVGTIFGRMGTAMSIPFLAIYLTQVKGASSGFAGAVIAASSLIGI 60 Query: 61 LISFYGGYISDVFGRKIVMLVSVFGWACVFFGFSSAQHIWVFFLVNTLNGLCRAVFEPTS 120 SFYGGY+SD FGRK +MLVS+FGW VF GF+ A H+W FF++N LNGLCRA+FEPTS Sbjct: 61 AASFYGGYLSDRFGRKTIMLVSIFGWTFVFAGFALADHVWTFFIMNALNGLCRALFEPTS 120 Query: 121 RALLSDITPPDHKLLVFNLRYAAVNLGVVFGPIIGLQLGSAKSTFPFVIAGVVYIAYGIV 180 RALLSD++ P+ +L VFNLRY A+NLGVVFGP++GL LGS+K+T PF IA +Y Y V Sbjct: 121 RALLSDVSKPETRLFVFNLRYTAINLGVVFGPLLGLYLGSSKTTLPFFIAAFIYFIYAAV 180 Query: 181 LFMQFSAHRASLPVRSEARAPKLREALAVTGRDRVFLPVLVGTVFCVLGYGHFSSTLAQY 240 L +QF H ++P + K+R+AL VT DRVF L+G++ C+ GY HF+STLAQY Sbjct: 181 LVIQFKHH--TVPAAERNKRIKVRDALEVTKTDRVFTITLIGSILCLFGYSHFTSTLAQY 238 Query: 241 LAVDTHFANGSQVFSYMLSLNAVTVLVVQYPIVRTASKFPPLVPLILGNICVALSLVLFG 300 +A +G++VF ML+LNA+ VL VQYPIV A +F P++ LI GN+ ++ SL Sbjct: 239 MAASPFIEDGTKVFGMMLTLNALAVLAVQYPIVHIAKRFSPILSLIAGNVLISGSLFSLS 298 Query: 301 VAGGVALLMFSVVLFTIGEVLLFTMMDMLIDRIAKPEWKGTYFGTIGFNNLGNVMAPVLG 360 + ++ +++FTIGEVLLF+MMD+L+DRIA+PE KGTYFG +GF LG+V+ P G Sbjct: 299 FFHDLWSIVLIIIVFTIGEVLLFSMMDLLVDRIARPELKGTYFGAMGFTQLGSVVGPWAG 358 Query: 361 GLLLDQFGATNGPAVFVPLALSTALGLPFLITAHKRLHARE 401 G+LLD FGA F L+ +T G+PFL+ A+++L E Sbjct: 359 GMLLDTFGAEKPFFTFAILSAATICGVPFLVAAYRKLKMDE 399 >A8FEV6.1 A8FEV6_BACP2 MFS family major facilitator transporter, sugar:cation symporter Length = 411 Score = 442 bits (1136), Expect = e-122, Method: Compositional matrix adjust. Identities = 213/410 (51%), Positives = 297/410 (72%), Gaps = 4/410 (0%) Query: 1 MKQHLQQIHPLAWTIIIGTMFGRLVTSMSIPFLSIYLTQVLGASHSQTGITVAVSSLAGV 60 MK +L+ +HPL+WTIIIGT+FGR+ TSMSIPFL++YLTQV GAS S G+ +AVSSL G+ Sbjct: 1 MKNNLRTLHPLSWTIIIGTIFGRMATSMSIPFLAVYLTQVKGASASSAGLIIAVSSLIGI 60 Query: 61 LISFYGGYISDVFGRKIVMLVSVFGWACVFFGFSSAQHIWVFFLVNTLNGLCRAVFEPTS 120 + SFYGGYISD GRK VML+S+FGW VF GF+ A IWVFF++N LNGLCRA+FEPTS Sbjct: 61 VASFYGGYISDRIGRKKVMLLSIFGWTLVFVGFAFADAIWVFFIMNALNGLCRALFEPTS 120 Query: 121 RALLSDITPPDHKLLVFNLRYAAVNLGVVFGPIIGLQLGSAKSTFPFVIAGVVYIAYGIV 180 +ALLSD+TP +L VFNLRY A+N+GV+FGP++GL LGS+K+TFPF++A +Y+ YG+ Sbjct: 121 KALLSDVTPSSIRLFVFNLRYTAINIGVIFGPLLGLYLGSSKTTFPFLVAAFIYLLYGLT 180 Query: 181 LFMQFSAHRASLPVRSEARAPKLREALAVTGRDRVFLPVLVGTVFCVLGYGHFSSTLAQY 240 L QF H + P + +++AL++ +D VF +L+G C GY H SSTL QY Sbjct: 181 LAWQFQKHPVAAP--ESTKQIGVKKALSIIQKDTVFSIILIGVTLCSFGYSHLSSTLPQY 238 Query: 241 LAVDTHFANGSQVFSYMLSLNAVTVLVVQYPIVRTASKFPPLVPLILGNICVALSLVLFG 300 L+ +G ++F Y+LSLNAV V+VVQYP++ A ++ ++ L GN+ +A SL Sbjct: 239 LSASPMIEDGPKLFGYLLSLNAVVVIVVQYPLIMIAKRYSTILSLTTGNVLLAGSLFAIA 298 Query: 301 VAGGVALLMFSVVLFTIGEVLLFTMMDMLIDRIAKPEWKGTYFGTIGFNNLGNVMAPVLG 360 + + +L F +V+FTIGEVLLF MMD+ +D +AKPE KG+YFG +GF+ LG+V+ P +G Sbjct: 299 FSQSLGMLAFIIVVFTIGEVLLFAMMDLFVDNVAKPEVKGSYFGAMGFSQLGSVIGPFVG 358 Query: 361 GLLLDQFGATNGPAVFVPLALSTALGLPFLITAHKRLHARERAEATTASQ 410 GL +D FGA + ++F L++ T G+PFL+ + RL ++R+ AT A + Sbjct: 359 GLCIDYFGAAHPFSIFSVLSIITIAGVPFLLIGYYRL--KKRSAATVAVK 406 >B4ALB9.1 B4ALB9_BACPU MFS family major facilitator transporter, sugar:cation symporter Length = 411 Score = 442 bits (1136), Expect = e-122, Method: Compositional matrix adjust. Identities = 212/403 (52%), Positives = 291/403 (72%), Gaps = 2/403 (0%) Query: 1 MKQHLQQIHPLAWTIIIGTMFGRLVTSMSIPFLSIYLTQVLGASHSQTGITVAVSSLAGV 60 MK +L+ +HPL+WTIIIGT+FGR+ TSMSIPFL++YLTQV GAS S G+ +AVSSL G+ Sbjct: 1 MKNNLRTLHPLSWTIIIGTIFGRMATSMSIPFLAVYLTQVKGASASSAGLIIAVSSLIGI 60 Query: 61 LISFYGGYISDVFGRKIVMLVSVFGWACVFFGFSSAQHIWVFFLVNTLNGLCRAVFEPTS 120 + SFYGGYISD GRK VML+S+FGW VF GF+ A IWVFF++N LNGLCRA+FEPTS Sbjct: 61 VASFYGGYISDRIGRKKVMLLSIFGWTLVFVGFAFADAIWVFFIMNALNGLCRALFEPTS 120 Query: 121 RALLSDITPPDHKLLVFNLRYAAVNLGVVFGPIIGLQLGSAKSTFPFVIAGVVYIAYGIV 180 +ALLSD+TP +L VFNLRY A+N+GV+FGP++GL LGS+K+TFPF++A +Y+ YG+ Sbjct: 121 KALLSDVTPSSIRLFVFNLRYTAINIGVIFGPLLGLYLGSSKTTFPFLVAAFIYLLYGLT 180 Query: 181 LFMQFSAHRASLPVRSEARAPKLREALAVTGRDRVFLPVLVGTVFCVLGYGHFSSTLAQY 240 L QF H + P + +++AL++ +D VF +L+G C GY H SSTL QY Sbjct: 181 LAWQFQKHPVAAP--ENTKQIGVKKALSIIQKDTVFSIILIGVTLCSFGYSHLSSTLPQY 238 Query: 241 LAVDTHFANGSQVFSYMLSLNAVTVLVVQYPIVRTASKFPPLVPLILGNICVALSLVLFG 300 L+ +G ++F Y+LSLNAV V+VVQYP++ A ++ ++ L GNI +A SL Sbjct: 239 LSASPMIEDGPKLFGYLLSLNAVVVIVVQYPLIMIAKRYSTILSLTTGNILLAGSLFAIA 298 Query: 301 VAGGVALLMFSVVLFTIGEVLLFTMMDMLIDRIAKPEWKGTYFGTIGFNNLGNVMAPVLG 360 + + +L F +V+FTIGEVLLF MMD+ +D +AKPE KG+YFG +GF+ LG+V+ P +G Sbjct: 299 FSQSLGMLAFIIVVFTIGEVLLFAMMDLFVDNVAKPEVKGSYFGAMGFSQLGSVIGPFVG 358 Query: 361 GLLLDQFGATNGPAVFVPLALSTALGLPFLITAHKRLHARERA 403 GL +D FGA + ++F L++ T G+PFL+ + RL R A Sbjct: 359 GLCIDYFGAAHPFSIFSVLSIITIAGVPFLLIGYYRLKKRSAA 401 >P54559.2 YQJV_BACSU Uncharacterized MFS-type transporter yqjV Length = 410 Score = 436 bits (1120), Expect = e-120, Method: Compositional matrix adjust. Identities = 218/407 (53%), Positives = 292/407 (71%), Gaps = 6/407 (1%) Query: 3 QHLQQIHPLAWTIIIGTMFGRLVTSMSIPFLSIYLTQVLGASHSQTGITVAVSSLAGVLI 62 + + IHP++WTIIIGT+FGR+ TSMSIPFL+IYLT V GAS S G+ +A SS G+L Sbjct: 2 KKWKDIHPISWTIIIGTIFGRMATSMSIPFLAIYLTAVQGASASYAGLVIAASSSVGILA 61 Query: 63 SFYGGYISDVFGRKIVMLVSVFGWACVFFGFSSAQHIWVFFLVNTLNGLCRAVFEPTSRA 122 SFYGGYISD FGRK +MLVS+FGW VF GF++A ++WVFF+VN LNGLC+++FEP S+A Sbjct: 62 SFYGGYISDKFGRKNMMLVSIFGWMLVFAGFAAASNLWVFFVVNALNGLCKSLFEPASKA 121 Query: 123 LLSDITPPDHKLLVFNLRYAAVNLGVVFGPIIGLQLGSAKSTFPFVIAGVVYIAYGIVLF 182 LLSD+T +LLVFNLRYAA+N+GVVFGP++GL GS++ST PF++ V+Y YGIVL Sbjct: 122 LLSDMTEEKTRLLVFNLRYAAINIGVVFGPVLGLYFGSSQSTTPFLVPAVIYGLYGIVLA 181 Query: 183 MQFSAHRASLPVRSEARAPKLREALAVTGRDRVFLPVLVGTVFCVLGYGHFSSTLAQYLA 242 +QF H SL +++R +REA VT +D +F LVG C GY FSST QY+A Sbjct: 182 LQFKKH-PSLSAPAQSRNMSVREAFMVTQKDYLFTIALVGITLCTFGYSQFSSTFPQYMA 240 Query: 243 VDTHFANGSQVFSYMLSLNAVTVLVVQYPIVRTASKFPPLVPLILGNICVALSLVLFGVA 302 + NG++++ ML+LNA+ VL Q+PIV A +F PL L+LGN+ V++S+ +F V+ Sbjct: 241 QNPLIGNGTKLYGLMLTLNAIVVLATQFPIVHFAKRFSPLCSLMLGNVMVSISMAIFTVS 300 Query: 303 GGVALLMFSVVLFTIGEVLLFTMMDMLIDRIAKPEWKGTYFGTIGFNNLGNVMAPVLGGL 362 GV ++ V+ FTIGEVLLF+MMD+ +D+IAKP KGTYFG IGF+ LGNV+ P +GG+ Sbjct: 301 HGVPSIVMIVITFTIGEVLLFSMMDLYVDQIAKPGLKGTYFGAIGFSQLGNVIGPWVGGI 360 Query: 363 LLDQFGATNGPAVFVPLALSTALGLPFLITAHKRLHA-----RERAE 404 +D FGA +F L+ T LGLPFL A++++ R R E Sbjct: 361 CIDLFGAGRPIYIFSVLSGITLLGLPFLAFAYRQMKMETTKHRSRLE 407 >Q733L7.1 Q733L7_BACC1 Conserved domain protein Length = 412 Score = 381 bits (978), Expect = e-104, Method: Compositional matrix adjust. Identities = 195/406 (48%), Positives = 262/406 (64%) Query: 1 MKQHLQQIHPLAWTIIIGTMFGRLVTSMSIPFLSIYLTQVLGASHSQTGITVAVSSLAGV 60 MK LQ IHPL TIIIGT+F R TSMSIPFL+IYLT V G S TG + S+L GV Sbjct: 1 MKGKLQNIHPLGMTIIIGTLFARFATSMSIPFLAIYLTTVKGVSAGMTGAIIGTSALVGV 60 Query: 61 LISFYGGYISDVFGRKIVMLVSVFGWACVFFGFSSAQHIWVFFLVNTLNGLCRAVFEPTS 120 SF GG +SD FGRK++ML S+ W VF GFS A H+ FFL+N LNGLCR+ FEPTS Sbjct: 61 FASFIGGNLSDRFGRKMIMLWSMIVWMFVFIGFSLADHVLSFFLLNALNGLCRSFFEPTS 120 Query: 121 RALLSDITPPDHKLLVFNLRYAAVNLGVVFGPIIGLQLGSAKSTFPFVIAGVVYIAYGIV 180 RALLSD+T P+++LLV+NLRY A+N+GV GPI+GLQ+GSAKST PF++A VYI Y + Sbjct: 121 RALLSDLTKPEYRLLVYNLRYGAINVGVAIGPIVGLQIGSAKSTIPFLVAAGVYILYTAI 180 Query: 181 LFMQFSAHRASLPVRSEARAPKLREALAVTGRDRVFLPVLVGTVFCVLGYGHFSSTLAQY 240 L QF + S + + A+ + +D VFL LVG + G+ H ++T++QY Sbjct: 181 LAFQFKKYPMEKKKVSTEKPVTMLNAMRILRKDIVFLVALVGIILSNCGFSHLTTTVSQY 240 Query: 241 LAVDTHFANGSQVFSYMLSLNAVTVLVVQYPIVRTASKFPPLVPLILGNICVALSLVLFG 300 A F +G +VFSYML+LNA+ V+V+QYP+++ K+ PLV +++G + V+ L FG Sbjct: 241 FANAHIFQDGVKVFSYMLALNAIVVVVIQYPVIQICKKYTPLVSIMVGTLFVSGGLFGFG 300 Query: 301 VAGGVALLMFSVVLFTIGEVLLFTMMDMLIDRIAKPEWKGTYFGTIGFNNLGNVMAPVLG 360 + ++FTIGEVL+F+M D+ ID IA KGTYFG +GF+ +G V+ P G Sbjct: 301 IIESTLGAAICTIIFTIGEVLMFSMTDVFIDDIAVAHLKGTYFGAMGFSGIGAVIGPWFG 360 Query: 361 GLLLDQFGATNGPAVFVPLALSTALGLPFLITAHKRLHARERAEAT 406 G+LLD +G NG VF LA+ + + P L+ L R + Sbjct: 361 GVLLDYYGYQNGFVVFSALAIFSTVAFPVLLVTKSLLKKRYDGNSN 406 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.329 0.142 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,302,519,043 Number of extensions: 94020561 Number of successful extensions: 417209 Number of sequences better than 1.0e-05: 3115 Number of HSP's gapped: 414932 Number of HSP's successfully gapped: 3133 Length of query: 412 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 274 Effective length of database: 1,760,596,133 Effective search space: 482403340442 Effective search space used: 482403340442 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)