BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0402.0019.p (222 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K6K5.1 B3K6K5_9BACI PspA/IM30 family protein 326 9e-88 B1D4Q7.1 B1D4Q7_9BACL Phage shock protein A, PspA 307 5e-82 A7GU73.1 A7GU73_BACCN Phage shock protein A, PspA 288 3e-76 C3BRI8.1 C3BRI8_9BACI Phage shock protein A, PspA 287 4e-76 C3B9J6.1 C3B9J6_BACMY Phage shock protein A, PspA 287 4e-76 >B3K6K5.1 B3K6K5_9BACI PspA/IM30 family protein Length = 222 Score = 326 bits (835), Expect = 9e-88, Method: Compositional matrix adjust. Identities = 176/220 (80%), Positives = 190/220 (86%) Query: 1 MSIFKRLRDLTMSNVNAIIDKAEDPVKMTDQYIRDMTEDLEDAEKAVAAQIAIEKKFKQL 60 MSIFKRLRDLTMSN+NAIIDKAEDP+KMTDQYIRDMTEDLEDAEKAVAAQIAIEK+FK L Sbjct: 1 MSIFKRLRDLTMSNINAIIDKAEDPIKMTDQYIRDMTEDLEDAEKAVAAQIAIEKRFKAL 60 Query: 61 YEEQEALVSKRNQQAHTAAQAGNADXXXXXXXXXXXXXXXXVEYKASFDQNKASADNLRG 120 YEEQ ALV KR QQAHTAAQA N + EYKAS+DQNK +ADNLRG Sbjct: 61 YEEQAALVEKRTQQAHTAAQAQNVELARRALEEKKAAEAKRDEYKASYDQNKLAADNLRG 120 Query: 121 KLEEMRKQLTQMKNKRETLVARYNAAKAQTEINKAMSGFSSDSASAGLKRMEDKMLAAEA 180 KLEEMRKQLT MKNKRETLVARYNAAKAQTEINKAM+GF SD+ASAGLKRME+KMLA EA Sbjct: 121 KLEEMRKQLTAMKNKRETLVARYNAAKAQTEINKAMNGFGSDTASAGLKRMEEKMLAMEA 180 Query: 181 QAEASNEMSSGNKSLDDEFEKLGKDQAVEDELAALMKQYD 220 +AEASNEMS+ KSLD+EFE LGKD+AVEDELAALMKQY+ Sbjct: 181 RAEASNEMSTREKSLDEEFENLGKDKAVEDELAALMKQYE 220 >B1D4Q7.1 B1D4Q7_9BACL Phage shock protein A, PspA Length = 224 Score = 307 bits (786), Expect = 5e-82, Method: Compositional matrix adjust. Identities = 164/223 (73%), Positives = 184/223 (82%), Gaps = 1/223 (0%) Query: 1 MSIFKRLRDLTMSNVNAIIDKAEDPVKMTDQYIRDMTEDLEDAEKAVAAQIAIEKKFKQL 60 MSIFKRLRDLT+SNV A+I+KAEDP+K+TDQYIRDM EDLEDAEKAVAAQIAIEKKFKQL Sbjct: 1 MSIFKRLRDLTLSNVYALIEKAEDPIKLTDQYIRDMQEDLEDAEKAVAAQIAIEKKFKQL 60 Query: 61 YEEQEALVSKRNQQAHTAAQAGNADXXXXXXXXXXXXXXXXVEYKASFDQNKASADNLRG 120 YEEQ LV KR +QAH AAQA N D EYK SFD NKA ADNLR Sbjct: 61 YEEQAQLVEKRGEQAHIAAQAQNIDLARRALEEKKAAEAKAAEYKTSFDNNKAMADNLRA 120 Query: 121 KLEEMRKQLTQMKNKRETLVARYNAAKAQTEINKAMSGFSSDSASAGLKRMEDKMLAAEA 180 KL+EMRK LT+MKNKRETLVARY AAKAQ +INK+++GFSSDSASAGLKRME+KML AEA Sbjct: 121 KLDEMRKALTEMKNKRETLVARYQAAKAQEQINKSLAGFSSDSASAGLKRMEEKMLQAEA 180 Query: 181 QAEASNEMS-SGNKSLDDEFEKLGKDQAVEDELAALMKQYDKQ 222 +AEASNE++ S KSLDDEF LGKD+AVEDELAALM+QY+K+ Sbjct: 181 RAEASNELAQSKGKSLDDEFAALGKDKAVEDELAALMQQYEKK 223 >A7GU73.1 A7GU73_BACCN Phage shock protein A, PspA Length = 250 Score = 288 bits (736), Expect = 3e-76, Method: Compositional matrix adjust. Identities = 148/221 (66%), Positives = 175/221 (79%) Query: 1 MSIFKRLRDLTMSNVNAIIDKAEDPVKMTDQYIRDMTEDLEDAEKAVAAQIAIEKKFKQL 60 MS+FKRLRDLTMSNV A+I+KAEDPVKMTDQY+RDM ED+++AEK+VAAQIA+EKKFK L Sbjct: 30 MSLFKRLRDLTMSNVYALIEKAEDPVKMTDQYLRDMQEDVQEAEKSVAAQIALEKKFKLL 89 Query: 61 YEEQEALVSKRNQQAHTAAQAGNADXXXXXXXXXXXXXXXXVEYKASFDQNKASADNLRG 120 +EEQEALV KR +QAH A QA N D EYKAS++QNKA+ADNLR Sbjct: 90 FEEQEALVKKREEQAHMAVQANNIDLARRALEEKQSAEQKMNEYKASYEQNKAAADNLRA 149 Query: 121 KLEEMRKQLTQMKNKRETLVARYNAAKAQTEINKAMSGFSSDSASAGLKRMEDKMLAAEA 180 KLEEMRKQLT++KNKRETLVAR NAAKAQ IN+AMSGF +++A AGL RME+K L EA Sbjct: 150 KLEEMRKQLTELKNKRETLVARVNAAKAQKSINQAMSGFDANTAKAGLSRMEEKALQLEA 209 Query: 181 QAEASNEMSSGNKSLDDEFEKLGKDQAVEDELAALMKQYDK 221 +AEAS E+ KSLD+EF L K+ AVEDELA +MKQY+K Sbjct: 210 EAEASGEIYKKEKSLDEEFAALNKNSAVEDELARIMKQYEK 250 >C3BRI8.1 C3BRI8_9BACI Phage shock protein A, PspA Length = 221 Score = 287 bits (735), Expect = 4e-76, Method: Compositional matrix adjust. Identities = 147/221 (66%), Positives = 175/221 (79%) Query: 1 MSIFKRLRDLTMSNVNAIIDKAEDPVKMTDQYIRDMTEDLEDAEKAVAAQIAIEKKFKQL 60 MS+FKRLRDLTMSNV A+I+KAEDPVKMTDQY+RDM ED+++AEK+VAAQIA+EKKFK L Sbjct: 1 MSVFKRLRDLTMSNVYALIEKAEDPVKMTDQYLRDMQEDVQEAEKSVAAQIALEKKFKLL 60 Query: 61 YEEQEALVSKRNQQAHTAAQAGNADXXXXXXXXXXXXXXXXVEYKASFDQNKASADNLRG 120 +EEQEALV KR +QAH A QA N D EYKAS++QNKA+ADNLR Sbjct: 61 FEEQEALVKKREEQAHMAVQANNIDLARRALEEKQNAEQKMNEYKASYEQNKAAADNLRA 120 Query: 121 KLEEMRKQLTQMKNKRETLVARYNAAKAQTEINKAMSGFSSDSASAGLKRMEDKMLAAEA 180 KLEEMRKQLT+++NKRETLVAR NAAKAQ IN+AMSGF +++A AGL RME+K L EA Sbjct: 121 KLEEMRKQLTELRNKRETLVARVNAAKAQKNINQAMSGFDANTAKAGLSRMEEKALQLEA 180 Query: 181 QAEASNEMSSGNKSLDDEFEKLGKDQAVEDELAALMKQYDK 221 +AEAS E+ KSLDDEF L K+ AV+DELA +MKQY+K Sbjct: 181 EAEASGEIYKKEKSLDDEFAALNKNSAVDDELARIMKQYEK 221 >C3B9J6.1 C3B9J6_BACMY Phage shock protein A, PspA Length = 221 Score = 287 bits (735), Expect = 4e-76, Method: Compositional matrix adjust. Identities = 147/221 (66%), Positives = 175/221 (79%) Query: 1 MSIFKRLRDLTMSNVNAIIDKAEDPVKMTDQYIRDMTEDLEDAEKAVAAQIAIEKKFKQL 60 MS+FKRLRDLTMSNV A+I+KAEDPVKMTDQY+RDM ED+++AEK+VAAQIA+EKKFK L Sbjct: 1 MSVFKRLRDLTMSNVYALIEKAEDPVKMTDQYLRDMQEDVQEAEKSVAAQIALEKKFKLL 60 Query: 61 YEEQEALVSKRNQQAHTAAQAGNADXXXXXXXXXXXXXXXXVEYKASFDQNKASADNLRG 120 +EEQEALV KR +QAH A QA N D EYKAS++QNKA+ADNLR Sbjct: 61 FEEQEALVKKREEQAHMAVQANNIDLARRALEEKQNAEQKMNEYKASYEQNKAAADNLRA 120 Query: 121 KLEEMRKQLTQMKNKRETLVARYNAAKAQTEINKAMSGFSSDSASAGLKRMEDKMLAAEA 180 KLEEMRKQLT+++NKRETLVAR NAAKAQ IN+AMSGF +++A AGL RME+K L EA Sbjct: 121 KLEEMRKQLTELRNKRETLVARVNAAKAQKNINQAMSGFDANTAKAGLSRMEEKALQLEA 180 Query: 181 QAEASNEMSSGNKSLDDEFEKLGKDQAVEDELAALMKQYDK 221 +AEAS E+ KSLDDEF L K+ AV+DELA +MKQY+K Sbjct: 181 EAEASGEIYKKEKSLDDEFAALNKNSAVDDELARIMKQYEK 221 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.307 0.120 0.301 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 934,663,302 Number of extensions: 29222702 Number of successful extensions: 164743 Number of sequences better than 1.0e-05: 750 Number of HSP's gapped: 164021 Number of HSP's successfully gapped: 751 Length of query: 222 Length of database: 3,060,696,203 Length adjustment: 131 Effective length of query: 91 Effective length of database: 1,826,543,238 Effective search space: 166215434658 Effective search space used: 166215434658 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.6 bits) S2: 128 (53.9 bits)