BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0402.0005.p (583 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K6L2.1 B3K6L2_9BACI Alpha amylase catalytic region 880 0.0 B1DH60.1 B1DH60_9BACL Alpha amylase catalytic region 717 0.0 Q84AP9.1 Q84AP9_9BACL Cyclodextrinase enzyme 629 e-178 B3K4I1.1 B3K4I1_9BACI Alpha amylase catalytic region 625 e-177 B1D8P8.1 B1D8P8_9BACL Alpha amylase catalytic region 622 e-176 >B3K6L2.1 B3K6L2_9BACI Alpha amylase catalytic region Length = 582 Score = 880 bits (2273), Expect = 0.0, Method: Compositional matrix adjust. Identities = 426/582 (73%), Positives = 489/582 (84%), Gaps = 2/582 (0%) Query: 1 MLLEAMYHVPRDKWAYAYDAKTIHLRVRTKRDDVDCVVALTGDKYNWQDTSYDIMMDKAA 60 MLLEA+YHVPRDKWAYAYD TIHLR+RTK++D+D VVA+TGDKY+W T ++I M+KAA Sbjct: 1 MLLEALYHVPRDKWAYAYDTHTIHLRIRTKKNDIDSVVAMTGDKYDWDRTYFEITMEKAA 60 Query: 61 SDDKFDYWEAAVRPKYKRLSYTFRVSKGNESVYLLDNGIQTEYPAPPNHYYEFPYIHQID 120 SDD FDYWE VRPKYKRLSY FR+ G+E+VY++D+GI +YP PP Y+EFPYIH++D Sbjct: 61 SDDMFDYWECGVRPKYKRLSYGFRIHAGDETVYMVDSGIGWDYPYPPAGYFEFPYIHEVD 120 Query: 121 LFQVPNWAKDAVFYQIMTERFANGDPANDPEGTEPWGGTPKLDNFFGGDLQGVLDHLDDL 180 +F+VP WAK+AVFYQIMTERFANG+PA P+GTE WGG P++DNFFGGDLQGVLDHLDDL Sbjct: 121 VFKVPEWAKEAVFYQIMTERFANGNPAISPDGTEAWGGKPEVDNFFGGDLQGVLDHLDDL 180 Query: 181 QELGINAIYFTPLFVSPSNHKYDIVDYKRVDPHFGDNELLKTVIEECHLRGIRVMLDAVF 240 +LGINAIYFTPLF+SPSNHKYD VDYK+VDPHFGDN+LLK V++ CH +GIRV+LDAVF Sbjct: 181 VDLGINAIYFTPLFLSPSNHKYDTVDYKQVDPHFGDNDLLKKVVDTCHAKGIRVILDAVF 240 Query: 241 NHCSDKFPPFQDVLEKGEYSVYKDWFHVNSFPAEIVGGIPTYDTFGFFGNMPKFNTANHE 300 NHCS++FPPFQDVL+ GE S Y WFHVN FP EI GIPTYDTFGFFGNMPKFNTAN E Sbjct: 241 NHCSEEFPPFQDVLKHGEKSKYAGWFHVNRFPCEIKDGIPTYDTFGFFGNMPKFNTANPE 300 Query: 301 VKTYLLEVAEYWIREIKADGWRLDVANEVDHHXXXXXXXXXXXXXXNPEAYIVGEVWSDS 360 VK YLL VAEYWI+EIK DGWRLDVANE+DHH NPEAYIVGEVWSDS Sbjct: 301 VKAYLLGVAEYWIKEIKLDGWRLDVANEIDHH--FWRDFRQVVKKANPEAYIVGEVWSDS 358 Query: 361 LTWLLGDQFDSVMNYPFSGTVLEFFNGGMDGITFGHRIGALLMRYPQQTNEVVFNMLGSH 420 L WL GDQFDSVMNYPF+ VLEFFNG MDG+TF +RIG++LMRYPQQTNEVVFN+L SH Sbjct: 359 LIWLQGDQFDSVMNYPFADKVLEFFNGNMDGLTFANRIGSILMRYPQQTNEVVFNLLCSH 418 Query: 421 DTPRLLTVVGEDKRRLKLAVVFLFTFMGTPCIYYGDEIGLTGHEDPDCRKCMEWDEASQD 480 DTPRLLT VGED RR+KL VVFLFTFMGTPCI+YGDEIGLTG DPDCRKCMEWD ++ Sbjct: 419 DTPRLLTRVGEDIRRMKLTVVFLFTFMGTPCIFYGDEIGLTGDGDPDCRKCMEWDPKKRN 478 Query: 481 RELYDFYKMMIALRKHNKALREGRFRILQACENDPCIVYERADDVIHFTVWMNNSPEPRI 540 +ELY FY+ MIALRK +KALR+GRFR LQA +DPCIVYERAD+ +HF VW+NNS R Sbjct: 479 QELYAFYQGMIALRKEHKALRQGRFRFLQANLHDPCIVYERADETMHFIVWINNSDWKRT 538 Query: 541 LSHPMETDDWQDALTGDPVAPEDGMMHISLDPYGYRILSRDL 582 LSHP+ T DW DA T +PV P +G MHI L+PY YRIL R+L Sbjct: 539 LSHPIVTTDWIDAETQEPVKPVNGTMHIDLEPYAYRILCREL 580 >B1DH60.1 B1DH60_9BACL Alpha amylase catalytic region Length = 582 Score = 717 bits (1852), Expect = 0.0, Method: Compositional matrix adjust. Identities = 334/583 (57%), Positives = 421/583 (72%), Gaps = 3/583 (0%) Query: 1 MLLEAMYHVPRDKWAYAYDAKTIHLRVRTKRDDVDCVVALTGDKYNWQDTSYDIMMDKAA 60 M E++YH P +W+YAYD T HLR++TKR DV+ VVA+ GDKY+W ++ M+ A Sbjct: 1 MQRESIYHSPSHRWSYAYDPGTFHLRLKTKRSDVERVVAIAGDKYDWDHHYQEVEMELIA 60 Query: 61 SDDKFDYWEAAVRPKYKRLSYTFRVSKGNESVYLLDNGIQTEYPAPPNHYYEFPYIHQID 120 +D DYWEA +RP++KR SY FR+ G E+ +L ++G E P P Y+E YIH ID Sbjct: 61 ADGMHDYWEAVIRPEFKRFSYGFRLHSGEETAWLTEDGFSEEQPTPAGGYFEAHYIHPID 120 Query: 121 LFQVPNWAKDAVFYQIMTERFANGDPANDPEGTEPWGGTPKLDNFFGGDLQGVLDHLDDL 180 LF+ P WAK+A+FYQI ERF NGD NDPEGT PWG P+ ++FFGGDLQG+++ + L Sbjct: 121 LFEAPEWAKEAIFYQIYPERFENGDTTNDPEGTSPWGEQPEGESFFGGDLQGIINRIGHL 180 Query: 181 QELGINAIYFTPLFVSPSNHKYDIVDYKRVDPHFGDNELLKTVIEECHLRGIRVMLDAVF 240 ELG+NA+Y TP+F SPSNHKYD DY+ VDPHFGD +LLK ++E CH GIRV+LDAVF Sbjct: 181 NELGVNAVYLTPVFRSPSNHKYDTTDYREVDPHFGDKDLLKMLVEVCHKHGIRVVLDAVF 240 Query: 241 NHCSDKFPPFQDVLEKGEYSVYKDWFHVNSFPAEIVGGIPTYDTFGFFGNMPKFNTANHE 300 NH S++FPPFQDVLEKG+ S +KDWFH+N FP E+ GI YDTFGF+GNMPK NTAN + Sbjct: 241 NHASEQFPPFQDVLEKGDQSEFKDWFHLNGFPVEVQDGIANYDTFGFYGNMPKLNTANPD 300 Query: 301 VKTYLLEVAEYWIREIKADGWRLDVANEVDHHXXXXXXXXXXXXXXNPEAYIVGEVWSDS 360 VK YL+E A W++E DGWRLDVANE+DHH N EA+I+GEVWSDS Sbjct: 301 VKNYLIETAVNWMKETGIDGWRLDVANEIDHH--FWRDFRAAIKDANKEAFIIGEVWSDS 358 Query: 361 LTWLLGDQFDSVMNYPFSGTVLEFF-NGGMDGITFGHRIGALLMRYPQQTNEVVFNMLGS 419 L WLLGDQFDSVMNYPF+ VL FF + + F ++ LLMRYPQQTNE +FN+L S Sbjct: 359 LRWLLGDQFDSVMNYPFTELVLSFFADRAIPPARFAEKMNRLLMRYPQQTNETLFNLLSS 418 Query: 420 HDTPRLLTVVGEDKRRLKLAVVFLFTFMGTPCIYYGDEIGLTGHEDPDCRKCMEWDEASQ 479 HD PR++T G D +RLK AVV++ T MG PC+YYGDE G+ G EDPDCR+CM W+E Q Sbjct: 419 HDIPRVMTRCGGDTKRLKQAVVYMMTSMGLPCVYYGDEFGMEGGEDPDCRRCMVWNEEDQ 478 Query: 480 DRELYDFYKMMIALRKHNKALREGRFRILQACENDPCIVYERADDVIHFTVWMNNSPEPR 539 +REL+DFYK++I+LRK ALR GRFR+L A E++ ++YER D HFT+WMN S EP Sbjct: 479 NRELFDFYKLLISLRKERAALRSGRFRVLLAEEDNGSLIYERLDGKEHFTIWMNRSDEPA 538 Query: 540 ILSHPMETDDWQDALTGDPVAPEDGMMHISLDPYGYRILSRDL 582 LSHPM W DALTG+ + DG + I L+P GYRI+ R + Sbjct: 539 ELSHPMNEGGWSDALTGERIDNPDGKLVIGLEPNGYRIVWRKI 581 >Q84AP9.1 Q84AP9_9BACL Cyclodextrinase enzyme Length = 653 Score = 629 bits (1621), Expect = e-178, Method: Compositional matrix adjust. Identities = 308/580 (53%), Positives = 386/580 (66%), Gaps = 4/580 (0%) Query: 1 MLLEAMYHVPRDKWAYAYDAKTIHLRVRTKRDDVDCVVALTGDKYNWQDTSYDIMMDKAA 60 M LEA+YH PR W+YAY+ T+HLR+RTK+DD+ V AL GDKY W T + M K A Sbjct: 1 MFLEAVYHRPRKNWSYAYNGTTVHLRIRTKKDDMTAVYALAGDKYMWDHTMEYVPMTKLA 60 Query: 61 SDDKFDYWEAAVRPKYKRLSYTFRVSKGNESVYLLDNGIQTEYPAPPNHYYEFPYIHQID 120 +D+ FDYWE V P Y+R+ Y F + +G+E ++ + TE PA P+ +E+P+I+ +D Sbjct: 61 TDELFDYWECEVTPPYRRVKYGFLLQQGHEKRWMTEYDFLTEPPANPDRLFEYPFINPVD 120 Query: 121 LFQVPNWAKDAVFYQIMTERFANGDPANDPEGTEPWGGT-PKLDNFFGGDLQGVLDHLDD 179 +FQ P W KDA+FYQI ERFANGD NDPEGT PWG P FFGGDLQGV+DHLD Sbjct: 121 VFQPPAWVKDAIFYQIFPERFANGDTRNDPEGTLPWGSADPTPSCFFGGDLQGVIDHLDH 180 Query: 180 LQELGINAIYFTPLFVSPSNHKYDIVDYKRVDPHFGDNELLKTVIEECHLRGIRVMLDAV 239 L +LG+NA+YFTPLF + +NHKYD DY ++DP FGD + LK +++ CH RGIRV+LDAV Sbjct: 181 LSKLGVNAVYFTPLFKATTNHKYDTEDYFQIDPQFGDKDTLKKLVDLCHERGIRVLLDAV 240 Query: 240 FNHCSDKFPPFQDVLEKGEYSVYKDWFHVNSFPAEIVGGIPTYDTFGFFGNMPKFNTANH 299 FNH FPPF DVL+ GE S YKDWFH+ S P E+V GIPTYDTF F MPK NT + Sbjct: 241 FNHSGRTFPPFVDVLKNGEKSKYKDWFHIRSLPLEVVDGIPTYDTFAFEPLMPKLNTEHP 300 Query: 300 EVKTYLLEVAEYWIREIKADGWRLDVANEVDHHXXXXXXXXXXXXXXNPEAYIVGEVWSD 359 EVK YLL+ AEYWIRE DGWRLDVANEV H NP+AYI+G+VW + Sbjct: 301 EVKEYLLKAAEYWIRETGIDGWRLDVANEVSHQ--FWREFRRVVKQANPDAYILGDVWHE 358 Query: 360 SLTWLLGDQFDSVMNYPFSGTVLEFF-NGGMDGITFGHRIGALLMRYPQQTNEVVFNMLG 418 S WL GDQFD+VMNYPF+ VL+FF + D F +G L YP+Q +EV+FN+L Sbjct: 359 SSIWLEGDQFDAVMNYPFTNAVLDFFIHQIADAEKFSFMLGKQLAGYPRQASEVMFNLLD 418 Query: 419 SHDTPRLLTVVGEDKRRLKLAVVFLFTFMGTPCIYYGDEIGLTGHEDPDCRKCMEWDEAS 478 SHDT RLLT DKR++KLAV+F FT+ GTPCIYYGDE+GL G DP CRKCMEWDE Sbjct: 419 SHDTARLLTQADGDKRKMKLAVLFQFTYFGTPCIYYGDEVGLDGGHDPGCRKCMEWDETK 478 Query: 479 QDRELYDFYKMMIALRKHNKALREGRFRILQACENDPCIVYERADDVIHFTVWMNNSPEP 538 D++L+ FY+ +I LR+ + ALR G F+ L A +N I Y R DD V MNN Sbjct: 479 HDKDLFAFYQTVIRLRQAHAALRTGTFKFLTAEKNSRQIAYLREDDQDTILVVMNNDKAG 538 Query: 539 RILSHPMETDDWQDALTGDPVAPEDGMMHISLDPYGYRIL 578 L+ P+ W D + G + + L YG+ +L Sbjct: 539 HTLTLPVRHAQWTHLWNDDVLTAAHGQLTVKLPAYGFAVL 578 >B3K4I1.1 B3K4I1_9BACI Alpha amylase catalytic region Length = 582 Score = 625 bits (1613), Expect = e-177, Method: Compositional matrix adjust. Identities = 307/581 (52%), Positives = 384/581 (66%), Gaps = 4/581 (0%) Query: 1 MLLEAMYHVPRDKWAYAYDAKTIHLRVRTKRDDVDCVVALTGDKYNWQDTSYDIMMDKAA 60 M LEA+YH PR W+YAY+ TIHLR+RTK+DD+ V AL GDKY W T I M K + Sbjct: 1 MFLEAIYHRPRKNWSYAYNGTTIHLRIRTKKDDLTEVYALAGDKYLWDQTMEYIPMTKLS 60 Query: 61 SDDKFDYWEAAVRPKYKRLSYTFRVSKGNESVYLLDNGIQTEYPAPPNHYYEFPYIHQID 120 SD+ FDYWE P Y+RL Y F + +G+E ++ + TE PA P+ +E+P+I+ D Sbjct: 61 SDEMFDYWECEATPPYRRLKYGFLLQQGDEKRWMTEYDFLTEPPANPDRLFEYPFINPDD 120 Query: 121 LFQVPNWAKDAVFYQIMTERFANGDPANDPEGTEPWGGT-PKLDNFFGGDLQGVLDHLDD 179 +F P W KDA+FYQI ERFANGD +NDPEG PWG P NFFGGDLQGV+DHLD Sbjct: 121 VFHPPAWVKDAIFYQIFPERFANGDTSNDPEGALPWGSAEPTPKNFFGGDLQGVIDHLDH 180 Query: 180 LQELGINAIYFTPLFVSPSNHKYDIVDYKRVDPHFGDNELLKTVIEECHLRGIRVMLDAV 239 L ELG+NAIYFTPLF + +NHKYD DY +DP FGD + LK +++ CH RGIRV+LDAV Sbjct: 181 LSELGVNAIYFTPLFTATTNHKYDTEDYMEIDPQFGDKDTLKKLVDLCHARGIRVLLDAV 240 Query: 240 FNHCSDKFPPFQDVLEKGEYSVYKDWFHVNSFPAEIVGGIPTYDTFGFFGNMPKFNTANH 299 FNH F PF DV + G S YKDWF+V S P E+V GIPTYDTF F MPK NT N Sbjct: 241 FNHSGRTFAPFVDVQKNGLNSKYKDWFYVRSLPLEVVDGIPTYDTFAFEPLMPKLNTENP 300 Query: 300 EVKTYLLEVAEYWIREIKADGWRLDVANEVDHHXXXXXXXXXXXXXXNPEAYIVGEVWSD 359 EV+ YLL+ AEYWI+E DGWRLDVANEV H NP+AYI+GEVW + Sbjct: 301 EVRAYLLKAAEYWIKETGIDGWRLDVANEVGH--SFWREFRQVVKKANPDAYILGEVWHE 358 Query: 360 SLTWLLGDQFDSVMNYPFSGTVLEFF-NGGMDGITFGHRIGALLMRYPQQTNEVVFNMLG 418 S WL GDQFD+VMNYPF+ VL+FF N D F +G L YP+Q +EV+FN+L Sbjct: 359 SSIWLEGDQFDAVMNYPFTNAVLDFFINRTADAEKFSFMLGKQLAGYPRQASEVMFNLLD 418 Query: 419 SHDTPRLLTVVGEDKRRLKLAVVFLFTFMGTPCIYYGDEIGLTGHEDPDCRKCMEWDEAS 478 SHDT RLLT DKR +KLA +F FT+ GTPCIYYGDEIG+ G DP CRKCMEWDE+ Sbjct: 419 SHDTARLLTQANGDKRLMKLAAMFQFTYFGTPCIYYGDEIGMDGGHDPGCRKCMEWDESK 478 Query: 479 QDRELYDFYKMMIALRKHNKALREGRFRILQACENDPCIVYERADDVIHFTVWMNNSPEP 538 D++L+ FY+ +I LRK + ALR G F+ L+A +N I Y R DD + +NN Sbjct: 479 HDKDLFHFYQTLIKLRKEHSALRTGTFKFLKAEKNGGKIAYLREDDNETILIAVNNDKAG 538 Query: 539 RILSHPMETDDWQDALTGDPVAPEDGMMHISLDPYGYRILS 579 ++ P+ W + G+ ++ + G + + L YG+ IL Sbjct: 539 NTMTLPVLDRQWTNLWNGEGLSAKQGQLTVKLPAYGFAILK 579 >B1D8P8.1 B1D8P8_9BACL Alpha amylase catalytic region Length = 583 Score = 622 bits (1603), Expect = e-176, Method: Compositional matrix adjust. Identities = 310/582 (53%), Positives = 384/582 (65%), Gaps = 9/582 (1%) Query: 1 MLLEAMYHVPRDKWAYAYDAKTIHLRVRTKRDDVDCVVALTGDKYNWQDTSYDIMMDKAA 60 MLLEA+YH P+ WAYAY+ T+H+R+RTK+ D V + GDKYNW T M + Sbjct: 1 MLLEAIYHRPKQNWAYAYNHTTLHIRIRTKKSDARSVELIAGDKYNWAGTFTITDMAVLS 60 Query: 61 SDDKFDYWEAAVRPKYKRLSYTFRVSKGNESVYLLDNGIQTEYPAPPNHYYEFPYIHQID 120 SD FDYWEA++ P ++RL Y FR++ G+E+V + ++G E P PN +++FPYI+ +D Sbjct: 61 SDALFDYWEASITPPFRRLRYAFRLTDGDETVIMTESGFPAELPDNPNTFFDFPYINPVD 120 Query: 121 LFQVPNWAKDAVFYQIMTERFANGDPANDPEGTEPWGGTPKLDNFFGGDLQGVLDHLDDL 180 +F P W KDAVFYQI ERFANGDP+ +PE TE WGG P NFFGGDLQGVLDHLD L Sbjct: 121 VFSPPAWVKDAVFYQIFPERFANGDPSLNPETTEAWGGKPTPTNFFGGDLQGVLDHLDYL 180 Query: 181 QELGINAIYFTPLFVSPSNHKYDIVDYKRVDPHFGDNELLKTVIEECHLRGIRVMLDAVF 240 ELGINA+YFTPLF + +NHKYD +Y +VD HFG NE LK ++EECH RGIR++LDAVF Sbjct: 181 AELGINAVYFTPLFEATTNHKYDTENYMQVDRHFGTNEKLKELVEECHKRGIRLLLDAVF 240 Query: 241 NHCSDKFPPFQDVLEKGEYSVYKDWFHVNSFPAEIVGGIPTYDTFGFFGNMPKFNTANHE 300 NH FPPF D+L+ GE SVY +WFHV +P I GIPTY+TF F MPK NT N + Sbjct: 241 NHAGRTFPPFADLLKNGEKSVYANWFHVREWPLRIEDGIPTYETFAFEPMMPKLNTENPD 300 Query: 301 VKTYLLEVAEYWIREIKADGWRLDVANEVDHHXXXXXXXXXXXXXXNPEAYIVGEVWSDS 360 VK YL VA YWI +I DGWRLDVANEVDH P+AYI+GE+W D+ Sbjct: 301 VKAYLFNVARYWIEKIGIDGWRLDVANEVDHQ--FWREFRQAVKSVKPDAYILGEIWHDA 358 Query: 361 LTWLLGDQFDSVMNYPFSGTVLEFF-NGGMDGITFGHRIGALLMRYPQQTNEVVFNMLGS 419 + WL GDQFD+VMNYPF+ VL+FF + ++G TF IG L YPQQ E FN+L S Sbjct: 359 MMWLEGDQFDAVMNYPFTNAVLDFFGHQTINGKTFADAIGRQLAAYPQQVTETAFNLLDS 418 Query: 420 HDTPRLLTVVGEDKRRLKLAVVFLFTFMGTPCIYYGDEIGLTGHEDPDCRKCMEWDEASQ 479 HDTPRLLT+ G+D +KLA +F T+ GTPCIYYGDE+G+ G DPDCRKCM W+ Q Sbjct: 419 HDTPRLLTICGDDADSMKLAALFQLTYPGTPCIYYGDEVGMDGDGDPDCRKCMVWEPEQQ 478 Query: 480 DRELYDFYKMMIALRKHNKALREGRFRILQAC--ENDPCIVYERADDVIHFTVWMNNSPE 537 DREL FYK +ALR + ALR R LQA E+ IVYER + V MN Sbjct: 479 DRELLAFYKQALALRSKHAALRSADIRFLQAADGEDGGVIVYERREGDERILVAMNARGR 538 Query: 538 PRILSHPMETDDWQDALTGDP--VAPEDGMMHISLDPYGYRI 577 + + P W +L GD + P G + + L YGY I Sbjct: 539 EAVAAVP-SGGSWV-SLLGDEQRLEPAAGKLQLRLPGYGYGI 578 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.141 0.453 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,943,241,894 Number of extensions: 182385819 Number of successful extensions: 334381 Number of sequences better than 1.0e-05: 4117 Number of HSP's gapped: 326252 Number of HSP's successfully gapped: 4265 Length of query: 583 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 442 Effective length of database: 1,732,333,088 Effective search space: 765691224896 Effective search space used: 765691224896 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 134 (56.2 bits)