BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0400.0007.p (302 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A6LYY4.1 A6LYY4_CLOB8 Major facilitator superfamily MFS_1 325 3e-87 B3RBV7.1 B3RBV7_CUPTR Drug resistance transporter, MFS family; m... 74 1e-11 Q0JZF9.1 Q0JZF9_RALEH MFS transporter, DHA2 family 73 3e-11 Q46V59.1 Q46V59_RALEJ Drug resistance transporter EmrB/QacA subf... 71 1e-10 Q1LGC2.1 Q1LGC2_RALME Drug resistance transporter EmrB/QacA subf... 68 9e-10 >A6LYY4.1 A6LYY4_CLOB8 Major facilitator superfamily MFS_1 Length = 415 Score = 325 bits (833), Expect = 3e-87, Method: Compositional matrix adjust. Identities = 160/296 (54%), Positives = 213/296 (71%), Gaps = 12/296 (4%) Query: 4 LKNKQLTSRSVTYIFAGLILINICANYISGSTGIYAATAFREYDRIDLLSILFVIEPLTR 63 +K K+L +F GL++INICA+Y+ GSTGIYAATAFREY +IDL + +FVIEPL R Sbjct: 1 MKAKRLNLDKADMVFIGLVIINICASYLVGSTGIYAATAFREYGKIDLFNAMFVIEPLAR 60 Query: 64 SAALLISGSAGEYFGRKKLYLWSVGAYALSIFLCALSPDGFLFLIAQGASGFFWGLFFSN 123 S +LLISG AGEYFGRK+LY+WSVGA+ALS+ + A + G +FLIA+GASGFFWGLFF+N Sbjct: 61 SISLLISGRAGEYFGRKQLYIWSVGAFALSVGISAAASSGTVFLIARGASGFFWGLFFAN 120 Query: 124 VFSILNDVVPEKEYPVRLATLQTVNFSVLIIGPIICGLLTEYWNWRMSLFALCPLFAAGL 183 VF+++ND+V E+EYPVR+ +QTV LI+GP++CG+ TE W+WR+SL L PLF G+ Sbjct: 121 VFTMINDIVSEEEYPVRIGIMQTVYSLTLILGPVLCGIFTERWSWRISLAILFPLFIIGM 180 Query: 184 ALIWFFLPDFNQR-----KFSEAQYAPQNIL-------KDLQISVLLKHRGFLIVSLITF 231 + FF+P + K S +Y L ++L I LLK +G++ + LITF Sbjct: 181 IFVGFFMPRSKTKNRIIIKSSIKEYDKGENLHINRSNKEELFIVKLLKQKGYVTIILITF 240 Query: 232 VFTCITCSGKYIPLYAQTALNSSATISALILLPCNLLGMLTVGLSGWYIKKKGCSK 287 + TCI+CSG YIPLYAQT L ++ TISA++L PCN+ M+ SG YI KKGC+K Sbjct: 241 IATCISCSGNYIPLYAQTVLGANTTISAVVLAPCNIFVMILSSASGIYISKKGCTK 296 >B3RBV7.1 B3RBV7_CUPTR Drug resistance transporter, MFS family; most similar to tcmA (Streptomyces glaucescens) Length = 531 Score = 74.3 bits (181), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 48/166 (28%), Positives = 78/166 (46%), Gaps = 17/166 (10%) Query: 71 GSAGEYFGRKKLYLWSVGAYALSIFLCALSPDGFLFLIAQGASGFFWGLFFSNVFSILND 130 G G+Y+GRK L S+ ++++ LC ++P+ + A+ G G+ F+ + D Sbjct: 77 GRLGDYYGRKPFVLASIVVFSVASALCGMAPNMPFLVAARALQGIGGGMLVGTAFACIAD 136 Query: 131 VVPEKEYPVRLATLQTVNFSVL-IIGPIICGLLTEYWNWRMSLFALCPLFAAGLALIWFF 189 + P+ +R + + F + IGP + GLLTE++ WR + P+ A GL W + Sbjct: 137 LFPDSHVRLRWQVMLSAAFGIANAIGPSLGGLLTEWYGWRAVFYVNLPVGALGLWFAWRY 196 Query: 190 LPDFNQRKFSEAQYAPQNI-----------LKDLQISV-LLKHRGF 223 LP Q +A P + L LQ+SV LL RG Sbjct: 197 LPHLRQ----QAHRGPIRVDWLGALLIAAGLGSLQMSVELLPARGL 238 >Q0JZF9.1 Q0JZF9_RALEH MFS transporter, DHA2 family Length = 531 Score = 73.2 bits (178), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 36/130 (27%), Positives = 65/130 (50%), Gaps = 1/130 (0%) Query: 71 GSAGEYFGRKKLYLWSVGAYALSIFLCALSPDGFLFLIAQGASGFFWGLFFSNVFSILND 130 G G+Y+GRK L S+ ++++ LC ++P ++A+ G G+ F+ + D Sbjct: 77 GRLGDYYGRKPFVLASIVVFSVASALCGMAPSMPFLVLARALQGIGGGMLVGTAFACIAD 136 Query: 131 VVPEKEYPVRLATLQTVNFSVL-IIGPIICGLLTEYWNWRMSLFALCPLFAAGLALIWFF 189 + P+ +R + + F + +GP + GLLTE++ WR + P+ GL W + Sbjct: 137 LFPDTHVRLRWQVMMSAAFGIANAVGPSLGGLLTEWYGWRAVFYVNLPIGVLGLWFAWRY 196 Query: 190 LPDFNQRKFS 199 LP Q+ S Sbjct: 197 LPHLRQQAHS 206 >Q46V59.1 Q46V59_RALEJ Drug resistance transporter EmrB/QacA subfamily Length = 532 Score = 71.2 bits (173), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 38/132 (28%), Positives = 65/132 (49%), Gaps = 1/132 (0%) Query: 69 ISGSAGEYFGRKKLYLWSVGAYALSIFLCALSPDGFLFLIAQGASGFFWGLFFSNVFSIL 128 I G G+Y+GRK L S+ ++++ LC ++ + A+G G G+ F+ + Sbjct: 75 IFGRLGDYYGRKPFVLASIVVFSVASALCGMATSMPFLVAARGLQGIGGGMLVGTAFACI 134 Query: 129 NDVVPEKEYPVRLATLQTVNFSVL-IIGPIICGLLTEYWNWRMSLFALCPLFAAGLALIW 187 D+ P+ +R L + F V IGP + G+LTE++ WR + P+ L +W Sbjct: 135 PDLFPDSHVRLRWQVLMSAAFGVANAIGPSLGGILTEWYGWRAVFYVNLPVGVLSLWFVW 194 Query: 188 FFLPDFNQRKFS 199 +LP Q+ S Sbjct: 195 RYLPHLRQQSHS 206 >Q1LGC2.1 Q1LGC2_RALME Drug resistance transporter EmrB/QacA subfamily Length = 548 Score = 68.2 bits (165), Expect = 9e-10, Method: Compositional matrix adjust. Identities = 49/183 (26%), Positives = 83/183 (45%), Gaps = 9/183 (4%) Query: 61 LTRSAALLISGSAGEYFGRKKLYLWSVGAYALSIFLCALSPDGFLFLIAQGASGFFWGLF 120 LT + I G G+Y+GRK L S+ + + LC ++ + + A+ G G+ Sbjct: 84 LTSVITVPIFGRLGDYYGRKPFVLASIVVFVAASALCGMATNMPFLVAARALQGIGGGML 143 Query: 121 FSNVFSILNDVVPEKEYPVRLATLQTVNFSVL-IIGPIICGLLTEYWNWRMSLFALCPLF 179 F+ + D+ P+ +R + + F + IGP + G+LTE++ WR + P+ Sbjct: 144 VGTAFACVPDLFPDPHVRLRWQVMMSTAFGLANAIGPSLGGVLTEWFGWRAVFYINLPVG 203 Query: 180 AAGLALIWFFLPDFNQRKFSE-------AQYAPQNILKDLQISVL-LKHRGFLIVSLITF 231 GL + W FLP + +E L LQ+SV L RG +V++ Sbjct: 204 LLGLLIAWRFLPHLRHQSHTEPIRIDWAGAALIAAGLGSLQLSVEWLPERGLGVVTIGLM 263 Query: 232 VFT 234 VF+ Sbjct: 264 VFS 266 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.329 0.143 0.447 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,667,262,628 Number of extensions: 66197826 Number of successful extensions: 237686 Number of sequences better than 1.0e-05: 488 Number of HSP's gapped: 241233 Number of HSP's successfully gapped: 488 Length of query: 302 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 167 Effective length of database: 1,788,859,178 Effective search space: 298739482726 Effective search space used: 298739482726 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 131 (55.1 bits)