BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0397.0021.p (394 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3EYL7.1 B3EYL7_BACST Two-component response regulator 142 6e-32 Q5KYP0.1 Q5KYP0_GEOKA Two-component response regulator 141 9e-32 Q65KZ3.1 Q65KZ3_BACLD YesN 114 2e-23 A8RP22.1 A8RP22_9CLOT Putative uncharacterized protein 113 3e-23 Q9K9C1.1 Q9K9C1_BACHD Two-component response regulator 111 2e-22 >B3EYL7.1 B3EYL7_BACST Two-component response regulator Length = 404 Score = 142 bits (358), Expect = 6e-32, Method: Compositional matrix adjust. Identities = 112/415 (26%), Positives = 192/415 (46%), Gaps = 46/415 (11%) Query: 1 MWNLLVVEDETIVRLGLRYMLDWDGLAISWKAEAANGQEALKALAQQEIHIVMTDIRMPG 60 +W +L+ +DE I+R G+R +DW+ + AEA +G+EAL+ + + ++ D+ MP Sbjct: 3 VWKVLIADDEAIIREGIRESIDWNEFNMEVVAEAEDGEEALELALRHRVDVLFVDLSMPI 62 Query: 61 MDGLELARRIKEQYSGVQIIFFSSFEDFPYVKEAVRIGVVDYLHKPTMDXXXXXXXXXXX 120 MDGL L + +E+ +I + +++F Y +EA+R+ V DYL KPT Sbjct: 63 MDGLTLMKYAREKLPNCHMIVITGYDEFSYAQEAIRLQVDDYLLKPTDPQRLREVVAKV- 121 Query: 121 XXXXXQLHQEAVPQAHTDKDRDHLLQQMLETEEHPADWAERWKRYGLEALYGQGY----- 175 +E + Q +K LL ++ H +++G+E + G+ Sbjct: 122 --------KEKLEQEQKEKQYRELLTNQVQKHIHVLR-----QQFGMEWVAGRLTEEDIM 168 Query: 176 -QLALLRIAENQPVELQAGLARFMSFR--------------YFLEEYTSREWGG--ILLS 218 QL+LL ++ + P Q + ++ SF+ + +E+ S G ++ Sbjct: 169 GQLSLLGLSRSCPA--QVVVIQWFSFKNQPIVSDRDRQLLLFAIEKAVSELLGDGEKIVF 226 Query: 219 RDNKELIWLLALDGERAEAGAMQAYLDQLEQSLFKMLGIRLSYTYSMIHRKAEELPEAYR 278 RD+ E I +++ E Q+E +L + L IR S + + AY Sbjct: 227 RDSNEFIVVISWKPISEEQ------CRQMETALKQHLHIRASVYIETVEGGMVGVATAYE 280 Query: 279 SAASQEPVSGGKLSGIIRLATAYIDGHLLE-DLTLARTAEEIHVSVSHLSRLFLKETGQH 337 Q V +LS I+R A YI H + DLTL AE ++VS +LSR+ +E G Sbjct: 281 KCKEQ-MVEESRLSPIVRRARQYIQKHFSQSDLTLESVAEFLNVSPVYLSRMIKQELGIS 339 Query: 338 FNEYVTXXXXXXXXXXXXNSNRKVYEVAEELGYANPHYFSKLFKDDTGMTPLEFR 392 F +T ++ ++E+AE +GY HYFS FK G++P ++R Sbjct: 340 FIHLLTKIRMEKAAELLLSTELPIHEIAERVGYDTQHYFSTAFKKAMGVSPNQYR 394 >Q5KYP0.1 Q5KYP0_GEOKA Two-component response regulator Length = 401 Score = 141 bits (356), Expect = 9e-32, Method: Compositional matrix adjust. Identities = 111/415 (26%), Positives = 192/415 (46%), Gaps = 46/415 (11%) Query: 1 MWNLLVVEDETIVRLGLRYMLDWDGLAISWKAEAANGQEALKALAQQEIHIVMTDIRMPG 60 MW +L+ +DE I+R G+R +DW+ + AEA +G+EAL+ + ++++ D+ MP Sbjct: 1 MWKVLIADDEAIIREGIRESIDWNEFNMEVVAEAEDGEEALELALRHRVNVLFVDLSMPI 60 Query: 61 MDGLELARRIKEQYSGVQIIFFSSFEDFPYVKEAVRIGVVDYLHKPTMDXXXXXXXXXXX 120 MDGL L + +E+ +I + +++F Y +EA+R+ V DYL KPT Sbjct: 61 MDGLTLMKYAREKLPNCHMIVITGYDEFSYAQEAIRLQVDDYLLKPTDPQRLREVVAKV- 119 Query: 121 XXXXXQLHQEAVPQAHTDKDRDHLLQQMLETEEHPADWAERWKRYGLEALYGQGY----- 175 +E + Q +K LL ++ H +++G+E + G+ Sbjct: 120 --------KEKLEQEQKEKQYRELLTNQVQKHIHVLR-----QQFGMEWVAGRLTEEDIM 166 Query: 176 -QLALLRIAENQPVELQAGLARFMSFR--------------YFLEEYTSREWGG--ILLS 218 QL+LL ++ + P Q + ++ SF+ + +E+ S G ++ Sbjct: 167 GQLSLLGLSRSCPA--QVVVIQWFSFKNQPIVSDRDRQLLLFAIEKAVSELLGDGEKIVF 224 Query: 219 RDNKELIWLLALDGERAEAGAMQAYLDQLEQSLFKMLGIRLSYTYSMIHRKAEELPEAYR 278 RD+ E I +++ E Q+E +L + L IR S + + AY Sbjct: 225 RDSNEFIVVISWKPISEEQ------CRQMETALKQHLHIRASVYIETVEGGMTGVMAAYE 278 Query: 279 SAASQEPVSGGKLSGIIRLATAYIDGHLLE-DLTLARTAEEIHVSVSHLSRLFLKETGQH 337 Q V +LS I+R A YI H + DLTL A+ ++VS +LSR+ +E G Sbjct: 279 KCKEQ-MVEESRLSPIVRRARQYIQKHFSQSDLTLESVAQFLNVSPVYLSRIIKQELGIS 337 Query: 338 FNEYVTXXXXXXXXXXXXNSNRKVYEVAEELGYANPHYFSKLFKDDTGMTPLEFR 392 F + ++ ++E+AE +GY HYFS FK G++P ++R Sbjct: 338 FIHLLAKTRMEKAAELLLSTELPIHEIAERVGYDTQHYFSTAFKKAMGVSPNQYR 392 >Q65KZ3.1 Q65KZ3_BACLD YesN Length = 388 Score = 114 bits (284), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 103/404 (25%), Positives = 181/404 (44%), Gaps = 31/404 (7%) Query: 1 MWNLLVVEDETIVRLGLRYMLDWDGLAISWKAEAANGQEALKALAQQEIHIVMTDIRMPG 60 M+ +L+ +DE I+ G+ M++W+ S A NG +A + + ++ IV++DI+MPG Sbjct: 1 MYKVLLADDERIILDGISSMIEWNSFGTSLIGAAQNGIDAYRLIMEKRPDIVISDIKMPG 60 Query: 61 MDGLELARRIKEQYSGVQIIFFSSFEDFPYVKEAVRIGVVDYLHKPTMDXXXXXXXXXXX 120 M GLEL ++ + ++ I + F F Y K+A+ GV YL KP + Sbjct: 61 MGGLELIEKVSRDHPSIRFILLTGFGQFEYAKKAMSYGVRHYLLKPCNESHITDSLQSVI 120 Query: 121 XXXXXQLHQEAVPQAHTDKD--RDHLLQQML-ETEEHPADWAERWKRYGLEALYGQGYQL 177 +E+ + TD + + HL++QML E E R+ E + +L Sbjct: 121 GELK---KEESAQKVKTDLEMMKPHLMKQMLKELITGGGIEDEACLRFFSEQ---KAIRL 174 Query: 178 ALLRIAENQPVELQAGLARFMSFRYFLEEYTSREWGGILLSRDNKELIWLLALDGERAEA 237 L + + + + G +E G +++ + + + A+DG A A Sbjct: 175 ILFSVRDGEDKDYAQG----------VESIADEVLNGAVIASARVQQLLVCAVDGTLASA 224 Query: 238 GAMQAYLDQLEQSLFKMLGIRLSYTYS---MIHRKAEELPEAYRSAASQEPVSGGKLSGI 294 ++ + +++ ++L + S I + +AYR E G+ S + Sbjct: 225 -VLEEKMKSVQELSRRLLRFEPEASLSESGNISEASAMYEKAYRKIVQDE----GETSVL 279 Query: 295 IRLATAYIDGHLLE-DLTLARTAEE-IHVSVSHLSRLFLKETGQHFNEYVTXXXXXXXXX 352 I I+ L+L A ++++ +L +LF +ETG+ F+ YV Sbjct: 280 IGRMLDLIEKEASNPKLSLKWAARNMLYMNPDYLGKLFKQETGEKFSSYVAKVRIEKAIA 339 Query: 353 XXXNS-NRKVYEVAEELGYA-NPHYFSKLFKDDTGMTPLEFRNQ 394 S N + +AEELG+ NP YFS +FK TG+TP EFR + Sbjct: 340 KMKKSKNTAIGTLAEELGFGHNPKYFSLVFKKYTGLTPSEFRKK 383 >A8RP22.1 A8RP22_9CLOT Putative uncharacterized protein Length = 525 Score = 113 bits (282), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 60/164 (36%), Positives = 91/164 (55%), Gaps = 2/164 (1%) Query: 1 MWNLLVVEDETIVRLGLRYMLDWDGLAISWKAEAANGQEALKALAQQEIHIVMTDIRMPG 60 M+ +L+ +DE IVR+ L M+DWD + + +NG+EAL L Q + I++TDI+MP Sbjct: 4 MYTVLIADDEAIVRMMLSSMIDWDEMELKLAGCVSNGREALAYLEQHPVDILITDIQMPV 63 Query: 61 MDGLELARRIKEQYSGVQIIFFSSFEDFPYVKEAVRIGVVDYLHKPTMDXXXXXXXXXXX 120 +DGLEL RR+KE ++ +I+ S++ DFPYV++A ++G+ DY K + Sbjct: 64 IDGLELIRRVKEFHTQPEILVLSAYNDFPYVRQAFKLGIYDYCLKREIHEEMLKRHLTNM 123 Query: 121 XXXXXQLHQEAVPQAHTDKDRDHLLQQML--ETEEHPADWAERW 162 Q + A KDR LL Q+L E E A ER+ Sbjct: 124 KQLLSQKGRTQTSGAEHVKDRKQLLAQLLCGELEPEAAGLPERY 167 >Q9K9C1.1 Q9K9C1_BACHD Two-component response regulator Length = 510 Score = 111 bits (277), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 49/109 (44%), Positives = 73/109 (66%) Query: 1 MWNLLVVEDETIVRLGLRYMLDWDGLAISWKAEAANGQEALKALAQQEIHIVMTDIRMPG 60 M N+L+V+DE ++R G+ DW+ EAANG++AL+ +++ E HIVMTDI MP Sbjct: 1 MCNVLIVDDEKLIRQGIINFFDWEKEGFQIVGEAANGKDALELISKNEPHIVMTDIVMPV 60 Query: 61 MDGLELARRIKEQYSGVQIIFFSSFEDFPYVKEAVRIGVVDYLHKPTMD 109 MDG++L +R+K + V+II SSFE+F YV+ GV DY+ KP ++ Sbjct: 61 MDGIDLVKRVKRDFPEVEIIVLSSFEEFEYVRSTFHSGVADYILKPRLN 109 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.135 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,172,739,181 Number of extensions: 81747183 Number of successful extensions: 278802 Number of sequences better than 1.0e-05: 6700 Number of HSP's gapped: 291075 Number of HSP's successfully gapped: 7158 Length of query: 394 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 256 Effective length of database: 1,760,596,133 Effective search space: 450712610048 Effective search space used: 450712610048 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)