BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0397.0008.p (562 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C5D5Y7.1 C5D5Y7_9BACI Alpha amylase catalytic region 800 0.0 P29094.1 O16G_BACTR Oligo-1,6-glucosidase EC=3.2.1.10 796 0.0 A7GS42.1 A7GS42_BACCN Alpha amylase catalytic region 779 0.0 C2WBL8.1 C2WBL8_BACCE Oligo-1,6-glucosidase 777 0.0 A6CSL5.1 A6CSL5_9BACI Oligo-1,6-glucosidase (Fragment) 774 0.0 >C5D5Y7.1 C5D5Y7_9BACI Alpha amylase catalytic region Length = 562 Score = 800 bits (2066), Expect = 0.0, Method: Compositional matrix adjust. Identities = 375/563 (66%), Positives = 453/563 (80%), Gaps = 4/563 (0%) Query: 1 MNKQWWKESVVYQIYPRSFQDSNGDGIGDLQGIISRLDYLEQLGINVVWLCPVYQSPNDD 60 M + WWKE+VVYQIYPRSF DSNGDGIGD++GII++LDYL++LG++VVWL PVY+SPNDD Sbjct: 1 MERAWWKEAVVYQIYPRSFYDSNGDGIGDIRGIIAKLDYLKELGVDVVWLSPVYKSPNDD 60 Query: 61 NGYDISNYQSIMNDFGTLADWEELLAGLHARGMKLIMDLVVNHSSDEHAWFAESRKSPDS 120 NGYDIS+Y+ IM++FGT+ DWEE+L +H RG+KL+MDLVVNH SDEH WF ESRKS D+ Sbjct: 61 NGYDISDYREIMDEFGTMEDWEEMLEEMHKRGIKLVMDLVVNHISDEHPWFIESRKSKDN 120 Query: 121 PYRDYYIWRAGKDGHEPNNWGSFFSGPAWEYDAQTDEYFLHLFSRKQPDLNWDNPKLRRE 180 PYRDYYIWR GKDG EPNNW S FSG AWEYD T+EY+LHLFS+KQPDLNW+NPK+RRE Sbjct: 121 PYRDYYIWRPGKDGKEPNNWESNFSGSAWEYDETTEEYYLHLFSKKQPDLNWENPKVRRE 180 Query: 181 IYDMMTWWLDKGIDGFRMDVINLISKVPELPSVVKDQEDGERSYHFGGEYFVNGPRVHEY 240 +YD+M +WLDKG+DGFRMDVIN+ISKVPELP K QE + Y G +YF+NGPRVHE+ Sbjct: 181 VYDIMKFWLDKGVDGFRMDVINMISKVPELPD-GKPQEG--KKYASGSKYFMNGPRVHEF 237 Query: 241 MQEMNREVLSKYDIMTVGEAIGVTPEEAALYVGEDRNELHMLFHFELMEVDSGPRGKWDV 300 +QEMNREVLSKYDIMTVGE GVTP+E LY R+EL+M+F FE + +DSGP GKWD+ Sbjct: 238 LQEMNREVLSKYDIMTVGETPGVTPKEGILYTDPSRHELNMVFQFEHVGLDSGPGGKWDI 297 Query: 301 RPWKLADIKSIFSKWQTALHGKGWNSLYLNNHDQPRMLSRFGDDKHYPKESAKMLATLLH 360 RPW LAD+K +KWQ L GKGWNSLYLNNHDQPR +SRFGDD Y ESAKMLAT LH Sbjct: 298 RPWSLADLKKTMTKWQKELEGKGWNSLYLNNHDQPRAVSRFGDDGKYRVESAKMLATFLH 357 Query: 361 TLQGTPYIYQGEEIGMTNVKFDSISEYKDIEILNMYKE-YRAAGHAEEEIMNSIYIKGRD 419 +QGTPYIYQGEEIGMTNV+F SI Y+DIE LNMYKE G +++M IY KGRD Sbjct: 358 MMQGTPYIYQGEEIGMTNVRFPSIEYYRDIETLNMYKERVEEYGEDPQKVMEKIYYKGRD 417 Query: 420 NGRTPMQWSAEPQAGFTTGTPWLAVNPNYTEINVEQALADPDSIFHYYKKLIELRKSHDI 479 N RTPMQW AGFTTGTPW+ VNPNY EINV++ALADP+S+FHYYKKLI+ RK HDI Sbjct: 418 NARTPMQWDDSENAGFTTGTPWIPVNPNYKEINVKEALADPNSVFHYYKKLIQFRKQHDI 477 Query: 480 FVYGDYAILAEEHEQVYAYLRTLGEERLLVMLNFFGRPAAFELPACVVFQDKTLVIANYG 539 VYG Y ++ E+ +YAY RTLG E+L+V+ NF + F LP + ++ K L+I+NY Sbjct: 478 IVYGTYDLILEDDPYIYAYTRTLGNEKLIVITNFSEKTPVFRLPDDITYKTKELLISNYD 537 Query: 540 VQPEEDIRSLSLRPYEARVYRLR 562 V E+++ + LRP+EARVY++R Sbjct: 538 VDETEELKEIRLRPWEARVYKIR 560 >P29094.1 O16G_BACTR Oligo-1,6-glucosidase EC=3.2.1.10 Length = 562 Score = 796 bits (2055), Expect = 0.0, Method: Compositional matrix adjust. Identities = 372/567 (65%), Positives = 451/567 (79%), Gaps = 12/567 (2%) Query: 1 MNKQWWKESVVYQIYPRSFQDSNGDGIGDLQGIISRLDYLEQLGINVVWLCPVYQSPNDD 60 M + WWKE+VVYQIYPRSF DSNGDGIGD++GII++LDYL++LG++VVWL PVY+SPNDD Sbjct: 1 MERVWWKEAVVYQIYPRSFYDSNGDGIGDIRGIIAKLDYLKELGVDVVWLSPVYKSPNDD 60 Query: 61 NGYDISNYQSIMNDFGTLADWEELLAGLHARGMKLIMDLVVNHSSDEHAWFAESRKSPDS 120 NGYDIS+Y+ IM++FGT+ADW+ +L +H RG+KL+MDLVVNH+SDEH WF ESRKS D+ Sbjct: 61 NGYDISDYRDIMDEFGTMADWKTMLEEMHKRGIKLVMDLVVNHTSDEHPWFIESRKSKDN 120 Query: 121 PYRDYYIWRAGKDGHEPNNWGSFFSGPAWEYDAQTDEYFLHLFSRKQPDLNWDNPKLRRE 180 PYRDYYIWR GK+G EPNNW S FSG AWEYD T EY+LHLFS+KQPDLNW+NPK+RRE Sbjct: 121 PYRDYYIWRPGKNGKEPNNWESVFSGSAWEYDEMTGEYYLHLFSKKQPDLNWENPKVRRE 180 Query: 181 IYDMMTWWLDKGIDGFRMDVINLISKVPELPSVVKDQEDGE----RSYHFGGEYFVNGPR 236 +Y+MM +WLDKG+DGFRMDVIN+ISKVPELP DGE + Y G Y++NGPR Sbjct: 181 VYEMMKFWLDKGVDGFRMDVINMISKVPELP-------DGEPQSGKKYASGSRYYMNGPR 233 Query: 237 VHEYMQEMNREVLSKYDIMTVGEAIGVTPEEAALYVGEDRNELHMLFHFELMEVDSGPRG 296 VHE++QEMNREVLSKYDIMTVGE GVTP+E LY R EL+M+F FE M++DSGP G Sbjct: 234 VHEFLQEMNREVLSKYDIMTVGETPGVTPKEGILYTDPSRRELNMVFQFEHMDLDSGPGG 293 Query: 297 KWDVRPWKLADIKSIFSKWQTALHGKGWNSLYLNNHDQPRMLSRFGDDKHYPKESAKMLA 356 KWD+RPW LAD+K +KWQ L GKGWNSLYLNNHDQPR +SRFGDD Y ESAKMLA Sbjct: 294 KWDIRPWSLADLKKTMTKWQKELEGKGWNSLYLNNHDQPRAVSRFGDDGKYRVESAKMLA 353 Query: 357 TLLHTLQGTPYIYQGEEIGMTNVKFDSISEYKDIEILNMYKE-YRAAGHAEEEIMNSIYI 415 T LH +QGTPYIYQGEEIGMTNV+F SI +Y+DIE LNMYKE G +E+M IY Sbjct: 354 TFLHMMQGTPYIYQGEEIGMTNVRFPSIEDYRDIETLNMYKERVEEYGEDPQEVMEKIYY 413 Query: 416 KGRDNGRTPMQWSAEPQAGFTTGTPWLAVNPNYTEINVEQALADPDSIFHYYKKLIELRK 475 KGRDN RTPMQW AGFT GTPW+ VNPNY EINV+ AL DP+S+FHYYKKLI+LRK Sbjct: 414 KGRDNARTPMQWDDSENAGFTAGTPWIPVNPNYKEINVKAALEDPNSVFHYYKKLIQLRK 473 Query: 476 SHDIFVYGDYAILAEEHEQVYAYLRTLGEERLLVMLNFFGRPAAFELPACVVFQDKTLVI 535 HDI VYG Y ++ E+ +Y Y RTLG E+L+V+ NF + F LP ++++ K L+I Sbjct: 474 QHDIIVYGTYDLILEDDPYIYRYTRTLGNEQLIVITNFSEKTPVFRLPDHIIYKTKELLI 533 Query: 536 ANYGVQPEEDIRSLSLRPYEARVYRLR 562 +NY V E+++ + LRP+EARVY++R Sbjct: 534 SNYDVDEAEELKEIRLRPWEARVYKIR 560 >A7GS42.1 A7GS42_BACCN Alpha amylase catalytic region Length = 559 Score = 779 bits (2011), Expect = 0.0, Method: Compositional matrix adjust. Identities = 369/563 (65%), Positives = 446/563 (79%), Gaps = 6/563 (1%) Query: 1 MNKQWWKESVVYQIYPRSFQDSNGDGIGDLQGIISRLDYLEQLGINVVWLCPVYQSPNDD 60 M KQWWKESVVYQIYPRSF DSNGDGIGDL+GIIS+LDYL++LGI+VVWL PVY+SPNDD Sbjct: 1 MTKQWWKESVVYQIYPRSFMDSNGDGIGDLRGIISKLDYLQKLGIDVVWLSPVYESPNDD 60 Query: 61 NGYDISNYQSIMNDFGTLADWEELLAGLHARGMKLIMDLVVNHSSDEHAWFAESRKSPDS 120 NGYDIS+Y+ IM +FGT+ DWEELL +H R +KL+MDLVVNH+SDEH WF ESRKS D+ Sbjct: 61 NGYDISDYRKIMTEFGTMEDWEELLHEMHKRNIKLMMDLVVNHTSDEHNWFIESRKSKDN 120 Query: 121 PYRDYYIWRAGKDGHEPNNWGSFFSGPAWEYDAQTDEYFLHLFSRKQPDLNWDNPKLRRE 180 PYRDYYIWR+GK+G EPNNW S FSG AW+YD TDEY+LHLFS+KQPDLNWDN ++R++ Sbjct: 121 PYRDYYIWRSGKEGKEPNNWESAFSGSAWQYDETTDEYYLHLFSKKQPDLNWDNEQVRKD 180 Query: 181 IYDMMTWWLDKGIDGFRMDVINLISKVPELPSVVKDQEDGERSYHFGGEYFVNGPRVHEY 240 +YDMM +W +KGIDGFRMDVIN ISK LP+V E E+ Y G +YF+NGP +H Y Sbjct: 181 VYDMMKFWFEKGIDGFRMDVINFISKEEGLPTV----ETEEKGYVSGHQYFMNGPNIHTY 236 Query: 241 MQEMNREVLSKYDIMTVGEAIGVTPEEAALYVGEDRNELHMLFHFELMEVDSGPRGKWDV 300 + EMNREVLSKYDIMTVGE GVT EEA LY E+R EL M+F FE M++DSG GKWD+ Sbjct: 237 LHEMNREVLSKYDIMTVGEMPGVTTEEAKLYTAEERQELQMVFQFEHMDLDSGEGGKWDI 296 Query: 301 RPWKLADIKSIFSKWQTALHGKGWNSLYLNNHDQPRMLSRFGDDKHYPKESAKMLATLLH 360 +P+ L +K +KWQ AL GWNSLY NNHDQPR++SRFG+D Y E+AKMLAT+LH Sbjct: 297 KPYSLLTLKQNLTKWQKALEYTGWNSLYWNNHDQPRVVSRFGNDTTYRIEAAKMLATVLH 356 Query: 361 TLQGTPYIYQGEEIGMTNVKFDSISEYKDIEILNMYKEYRAA-GHAEEEIMNSIYIKGRD 419 ++GTPYIYQGEEIGMTNV+FDSI+EY+DIE LNMY+E G EE++M SIY+KGRD Sbjct: 357 MMKGTPYIYQGEEIGMTNVRFDSINEYRDIETLNMYREKVIERGENEEQVMQSIYVKGRD 416 Query: 420 NGRTPMQWSAEPQAGFTTGTPWLAVNPNYTEINVEQALADPDSIFHYYKKLIELRKSHDI 479 N RTPMQW+ + AGFTTG PW+ VNPNY EINVEQAL D +SIF+YYKKLIELRK H+I Sbjct: 417 NARTPMQWNQDKHAGFTTGEPWIRVNPNYKEINVEQALQDSESIFYYYKKLIELRKKHEI 476 Query: 480 FVYGDYAILAEEHEQVYAYLRTLGEERLLVMLNFFGRPAAFELPACVVFQDKTLVIANYG 539 VYG Y ++ E H ++AY+RT +E+LLV+ NF FELP + + LVI NY Sbjct: 477 IVYGTYDLILENHPSIFAYVRTWKDEQLLVIANFTENECLFELPKEITYYLSELVIHNYD 536 Query: 540 VQPEEDIRSLSLRPYEARVYRLR 562 VQ + + S L+PYEARVY+L+ Sbjct: 537 VQ-DGTLESFVLQPYEARVYKLK 558 >C2WBL8.1 C2WBL8_BACCE Oligo-1,6-glucosidase Length = 565 Score = 777 bits (2007), Expect = 0.0, Method: Compositional matrix adjust. Identities = 369/563 (65%), Positives = 446/563 (79%), Gaps = 6/563 (1%) Query: 1 MNKQWWKESVVYQIYPRSFQDSNGDGIGDLQGIISRLDYLEQLGINVVWLCPVYQSPNDD 60 MNKQWWKESVVYQIYPRSF DSN DGIGDL+GIIS+LDYL+ LGI+V+WL PVY+SPNDD Sbjct: 7 MNKQWWKESVVYQIYPRSFMDSNSDGIGDLRGIISKLDYLQNLGIDVIWLSPVYESPNDD 66 Query: 61 NGYDISNYQSIMNDFGTLADWEELLAGLHARGMKLIMDLVVNHSSDEHAWFAESRKSPDS 120 NGYDISNY+ IM++FGT+ DW++LL+ +H R MKL+MDLVVNH+SDEH WF ESRKS D+ Sbjct: 67 NGYDISNYRKIMDEFGTMEDWDKLLSEMHKRNMKLMMDLVVNHTSDEHNWFVESRKSKDN 126 Query: 121 PYRDYYIWRAGKDGHEPNNWGSFFSGPAWEYDAQTDEYFLHLFSRKQPDLNWDNPKLRRE 180 YRDYYIWR KDG EPNNWG+ FSG AW+YD TDEY+LHLFS+KQPDLNW+N +R++ Sbjct: 127 LYRDYYIWRPAKDGKEPNNWGAAFSGSAWQYDEATDEYYLHLFSKKQPDLNWENEVVRKD 186 Query: 181 IYDMMTWWLDKGIDGFRMDVINLISKVPELPSVVKDQEDGERSYHFGGEYFVNGPRVHEY 240 +Y+MM +WL+KGIDGFRMDVIN ISK LPSV E E Y G +YF+NGP +H Y Sbjct: 187 VYEMMEFWLEKGIDGFRMDVINFISKTEGLPSV----ETKEEGYVSGHQYFMNGPNIHTY 242 Query: 241 MQEMNREVLSKYDIMTVGEAIGVTPEEAALYVGEDRNELHMLFHFELMEVDSGPRGKWDV 300 + EMN++VLSKYDIMTVGE GVT EEA LY EDR EL M+F FE M++DSG GKWDV Sbjct: 243 LHEMNQKVLSKYDIMTVGEMPGVTTEEARLYTAEDRQELQMVFQFEHMDLDSGEGGKWDV 302 Query: 301 RPWKLADIKSIFSKWQTALHGKGWNSLYLNNHDQPRMLSRFGDDKHYPKESAKMLATLLH 360 +P L +K +KWQ AL GWNSLY NNHDQPR++SRFGDD Y ESAKMLAT+LH Sbjct: 303 KPCSLVTLKQNLTKWQKALEQTGWNSLYWNNHDQPRVVSRFGDDTTYRIESAKMLATVLH 362 Query: 361 TLQGTPYIYQGEEIGMTNVKFDSISEYKDIEILNMYKE-YRAAGHAEEEIMNSIYIKGRD 419 ++GTPYIYQGEEIGMTNV FDSI EY+DIE LNMY+E G EE++M SIYIKGRD Sbjct: 363 MMKGTPYIYQGEEIGMTNVHFDSIDEYRDIETLNMYREKVIELGEDEEKVMQSIYIKGRD 422 Query: 420 NGRTPMQWSAEPQAGFTTGTPWLAVNPNYTEINVEQALADPDSIFHYYKKLIELRKSHDI 479 N RTPMQW+A AGFT G PW+ +NPNY EINVEQAL D +SIF+YYKKLIELRK H++ Sbjct: 423 NARTPMQWNANEHAGFTAGEPWIGLNPNYKEINVEQALQDQESIFYYYKKLIELRKKHEM 482 Query: 480 FVYGDYAILAEEHEQVYAYLRTLGEERLLVMLNFFGRPAAFELPACVVFQDKTLVIANYG 539 VYG Y ++ EEH ++AY+RT +E+LLV+ NF + FELP +++ D L+I NY Sbjct: 483 IVYGTYDLILEEHPSIFAYVRTWRDEKLLVIANFTSDESVFELPEEIMYGDIELLIHNYN 542 Query: 540 VQPEEDIRSLSLRPYEARVYRLR 562 VQ +E I S++L+PYE RVY+++ Sbjct: 543 VQ-DEVIESIALQPYETRVYKVK 564 >A6CSL5.1 A6CSL5_9BACI Oligo-1,6-glucosidase (Fragment) Length = 544 Score = 774 bits (1999), Expect = 0.0, Method: Compositional matrix adjust. Identities = 363/547 (66%), Positives = 443/547 (80%), Gaps = 4/547 (0%) Query: 1 MNKQWWKESVVYQIYPRSFQDSNGDGIGDLQGIISRLDYLEQLGINVVWLCPVYQSPNDD 60 M KQWWKESV+YQIYPRSF+DSNGDGIGD+ GIIS+LDYL++LGI+V+WL PVYQSPNDD Sbjct: 1 MTKQWWKESVIYQIYPRSFKDSNGDGIGDINGIISKLDYLKELGIDVIWLSPVYQSPNDD 60 Query: 61 NGYDISNYQSIMNDFGTLADWEELLAGLHARGMKLIMDLVVNHSSDEHAWFAESRKSPDS 120 NGYDIS+Y+ IM++FGT+ADW+ LL +H RG+KLIMDLVVNHSSDEHAWF ESRKS D+ Sbjct: 61 NGYDISDYRGIMDEFGTMADWDRLLEEMHNRGIKLIMDLVVNHSSDEHAWFVESRKSKDN 120 Query: 121 PYRDYYIWRAGKDGHEPNNWGSFFSGPAWEYDAQTDEYFLHLFSRKQPDLNWDNPKLRRE 180 PYRDYYIWR GKDG EPNNW S FSG AW+YD QT+EYFLHLFS+KQPDLNW+NPKLR E Sbjct: 121 PYRDYYIWREGKDGKEPNNWESCFSGSAWQYDEQTEEYFLHLFSKKQPDLNWENPKLRDE 180 Query: 181 IYDMMTWWLDKGIDGFRMDVINLISKVPELPSVVKDQEDGERSYHFGGEYFVNGPRVHEY 240 IYDMM +WLDKGIDGFRMDVIN ISKV LP Q + Y GG+YF+NGP++H+Y Sbjct: 181 IYDMMKFWLDKGIDGFRMDVINFISKVDGLPDAELPQ---GKKYAPGGKYFMNGPKIHDY 237 Query: 241 MQEMNREVLSKYDIMTVGEAIGVTPEEAALYVGEDRNELHMLFHFELMEVDSGPRGKWDV 300 +QEM+ + LS YD+MTVGE GV EEA LY EDR E++M+F FE +++DSGP GKWD+ Sbjct: 238 LQEMHEKALSGYDVMTVGEMPGVDVEEAKLYTAEDRKEVNMVFQFEHVDLDSGPDGKWDL 297 Query: 301 RPWKLADIKSIFSKWQTALHGKGWNSLYLNNHDQPRMLSRFGDDKHYPKESAKMLATLLH 360 RP K++ ++ F+KWQ L +GWNSLYLNNHDQPRM+SRFG+DK Y ESAKMLAT LH Sbjct: 298 RPMKMSALRDSFTKWQKGLEQEGWNSLYLNNHDQPRMVSRFGNDKEYRVESAKMLATFLH 357 Query: 361 TLQGTPYIYQGEEIGMTNVKFDSISEYKDIEILNMYKEYRAA-GHAEEEIMNSIYIKGRD 419 TL+GTPYIYQGEE+GMTNV+F+SI++YKDIEILNMY+E G +++M SIY+KGRD Sbjct: 358 TLKGTPYIYQGEELGMTNVRFNSINDYKDIEILNMYREKVIENGEDHDKVMESIYVKGRD 417 Query: 420 NGRTPMQWSAEPQAGFTTGTPWLAVNPNYTEINVEQALADPDSIFHYYKKLIELRKSHDI 479 N RTP+QW GFTTGTPW+ +NPNY EIN +QA+ DP+S+FHYYKKLIELRK + I Sbjct: 418 NARTPIQWDDSDHGGFTTGTPWIQMNPNYKEINAKQAMEDPNSVFHYYKKLIELRKENPI 477 Query: 480 FVYGDYAILAEEHEQVYAYLRTLGEERLLVMLNFFGRPAAFELPACVVFQDKTLVIANYG 539 VYG Y ++ EH+++YAY+R +++++VMLNF G FELPA + K L I+NY Sbjct: 478 MVYGSYDLIMPEHDKIYAYVREYEDQKMIVMLNFSGDTPVFELPAELEGGTKELFISNYN 537 Query: 540 VQPEEDI 546 V +EDI Sbjct: 538 VDADEDI 544 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.137 0.431 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,773,539,372 Number of extensions: 170950552 Number of successful extensions: 383133 Number of sequences better than 1.0e-05: 4074 Number of HSP's gapped: 371951 Number of HSP's successfully gapped: 4475 Length of query: 562 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 421 Effective length of database: 1,732,333,088 Effective search space: 729312230048 Effective search space used: 729312230048 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)