BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0397.0006.p (260 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B5XQS3.1 B5XQS3_KLEP3 Conserved domain protein 226 1e-57 A6T918.1 A6T918_KLEP7 Putative uncharacterized protein 219 2e-55 C4X8A6.1 C4X8A6_KLEPN Putative uncharacterized protein 217 7e-55 B3XMZ6.1 B3XMZ6_LACRE Putative uncharacterized protein 167 7e-40 C2F652.1 C2F652_LACRE Rhodanese domain protein 166 1e-39 >B5XQS3.1 B5XQS3_KLEP3 Conserved domain protein Length = 251 Score = 226 bits (577), Expect = 1e-57, Method: Compositional matrix adjust. Identities = 115/258 (44%), Positives = 153/258 (59%), Gaps = 7/258 (2%) Query: 1 MSSLQWDHTVHYINDLEQAVQTFTDNGLAAFPGGSHKLWGTHNALSYFGLNYVEFLAVEN 60 M++LQWDH V ++N + A+QTF D L A GG H WGT NALSYFGL Y+EFLA+ + Sbjct: 1 MATLQWDHAVQFVNQPDAAIQTFADQQLRAVAGGRHPGWGTRNALSYFGLTYIEFLAISD 60 Query: 61 RELAESADTANLVVKDAVKLLPEHEGFSRVAIRTDDIEQTAALLQQQGLKLSPIMDGRRL 120 + +A L+ +DA +LLPE+E RVA+R+DDIE T L+++GL +SPI+DG+R Sbjct: 61 PDELRAATDRFLLSRDAERLLPENEALFRVALRSDDIEATHDQLRRKGLAVSPIVDGQRN 120 Query: 121 NKQGQWIEWRMLTIGGHFQGLVYPFVIQWKGTDGQRQQELRTAGIIRPHPAGAVEVRAAV 180 + QG I WR+ TI G GLVYPFV+QW D R LR + PHP G + + AV Sbjct: 121 DPQGNIIRWRIFTIDGDTDGLVYPFVLQWGEDDTTRLARLRAQELDVPHPLGDIALEQAV 180 Query: 181 FSVTDPAATAAHWSQVIGLPFQNHRSAADGSVSLAIGNKAFVFRQGNGEQMQELLLATDA 240 F V +P A W ++G P Q G L +G + F FR+G Q+ EL+ Sbjct: 181 FEVVNPQAVRDRWQALLGFPPQ-------GEQGLDVGGQWFTFREGPANQLTELVFRVAN 233 Query: 241 PGLAGRRIRFGQADYVFT 258 P L G+R R G Y FT Sbjct: 234 PALKGQRFRVGNGVYRFT 251 >A6T918.1 A6T918_KLEP7 Putative uncharacterized protein Length = 251 Score = 219 bits (559), Expect = 2e-55, Method: Compositional matrix adjust. Identities = 110/258 (42%), Positives = 150/258 (58%), Gaps = 7/258 (2%) Query: 1 MSSLQWDHTVHYINDLEQAVQTFTDNGLAAFPGGSHKLWGTHNALSYFGLNYVEFLAVEN 60 M++LQWDH V ++N E A++ F L A GG H WGT NALSYFGL Y+EFLA+ + Sbjct: 1 MATLQWDHAVQFVNQPEAAIEIFAGQQLRAVAGGRHPGWGTRNALSYFGLTYIEFLAIAD 60 Query: 61 RELAESADTANLVVKDAVKLLPEHEGFSRVAIRTDDIEQTAALLQQQGLKLSPIMDGRRL 120 + +A L+ +DA +LLPE+E RVA+R+DDI+ T L++ G+ +SPI+DG+R Sbjct: 61 PDELRAATDKFLLSRDAARLLPENEALFRVALRSDDIDATHDQLRRTGVTVSPIVDGQRN 120 Query: 121 NKQGQWIEWRMLTIGGHFQGLVYPFVIQWKGTDGQRQQELRTAGIIRPHPAGAVEVRAAV 180 + QG I WR+ TI G GLVYPFV+QW D R LR + PHP G + + AV Sbjct: 121 DPQGNIIRWRIFTIDGDTAGLVYPFVLQWGEDDATRLTRLRAQRLDAPHPLGDITLEQAV 180 Query: 181 FSVTDPAATAAHWSQVIGLPFQNHRSAADGSVSLAIGNKAFVFRQGNGEQMQELLLATDA 240 F V +P A W ++G P G L +G + F+FR+G Q+ EL+ Sbjct: 181 FEVVNPQAVRDRWQALLGFP-------PLGEQGLDVGGQQFIFREGAANQLTELVFRVAN 233 Query: 241 PGLAGRRIRFGQADYVFT 258 P L G+R R G Y FT Sbjct: 234 PALKGQRFRVGNGVYRFT 251 >C4X8A6.1 C4X8A6_KLEPN Putative uncharacterized protein Length = 251 Score = 217 bits (553), Expect = 7e-55, Method: Compositional matrix adjust. Identities = 110/258 (42%), Positives = 150/258 (58%), Gaps = 7/258 (2%) Query: 1 MSSLQWDHTVHYINDLEQAVQTFTDNGLAAFPGGSHKLWGTHNALSYFGLNYVEFLAVEN 60 M++LQWDH V ++N E A++ F L A GG H WGT NALSYFGL Y+EFLA+ + Sbjct: 1 MATLQWDHAVQFVNQPEAAIEIFAGQQLWAVAGGHHPGWGTRNALSYFGLTYIEFLAIAD 60 Query: 61 RELAESADTANLVVKDAVKLLPEHEGFSRVAIRTDDIEQTAALLQQQGLKLSPIMDGRRL 120 + +A L+ +DA +LLPE+E RVA+R+DDI+ T L++ G+ +SPI+DG+R Sbjct: 61 PDELRAATDKFLLSRDAARLLPENEALFRVALRSDDIDATHDQLRRTGVTVSPIVDGQRN 120 Query: 121 NKQGQWIEWRMLTIGGHFQGLVYPFVIQWKGTDGQRQQELRTAGIIRPHPAGAVEVRAAV 180 + QG I WR+ TI G GLVYPFV+QW D R LR + PHP G + + AV Sbjct: 121 DPQGNIIRWRIFTIDGDTAGLVYPFVLQWGEDDATRLTRLRAQRLDAPHPLGDITLEQAV 180 Query: 181 FSVTDPAATAAHWSQVIGLPFQNHRSAADGSVSLAIGNKAFVFRQGNGEQMQELLLATDA 240 F V +P A W ++G P G L +G + F+FR+G Q+ EL+ Sbjct: 181 FVVVNPQAVRDRWQALLGFP-------PLGEQGLDVGGQQFIFREGAANQLTELVFRVAN 233 Query: 241 PGLAGRRIRFGQADYVFT 258 P L G+R R G Y FT Sbjct: 234 PALKGQRFRVGNGVYRFT 251 >B3XMZ6.1 B3XMZ6_LACRE Putative uncharacterized protein Length = 258 Score = 167 bits (423), Expect = 7e-40, Method: Compositional matrix adjust. Identities = 94/258 (36%), Positives = 145/258 (56%), Gaps = 9/258 (3%) Query: 2 SSLQWDHTVHYINDLEQAVQTFTDNGLAAFPGGSHKLWGTHNALSYFGLNYVEFLAVENR 61 +L WDHT+ + +L+ A+ F GLA PGG H+ WGT NAL YFGLNY+E L V N+ Sbjct: 3 ETLNWDHTMVDVTNLQDAIDYFNSKGLAFTPGGHHEYWGTKNALDYFGLNYIELLTVANK 62 Query: 62 ELAESADTA-NLVVKDAVK-LLPEHEGFSRVAIRTDDIEQTAALLQQQGLKLSPIMDGRR 119 E A++ N + DAV+ + + +AIRTD+IE+T L+ GL + I DG+R Sbjct: 63 EKAKAFPYENNSAIHDAVEDYFAGIQRITTIAIRTDNIEETHRRLKGLGLNVGDITDGKR 122 Query: 120 LNKQGQWIEWRMLTIGGHF-QGLVYPFVIQWKGTDGQRQQELRTAGIIRPHPAGAVEVRA 178 ++ G+ I+W++ + L PF IQW D +R+++L+ G+I+ HPAG + V+ Sbjct: 123 IDPAGKLIQWKIFYVDDELVNHLPAPFFIQWSEDDDKRREDLKKQGLIKFHPAGDLFVKQ 182 Query: 179 AVFSVTDPAATAAHWSQVIGLPFQNHRSAADGSVSLAIGNKAFVFRQGNGEQMQELLL-- 236 A+F+V+DP A WS ++ Q + + ++ IGNK VF G ++ +L+ Sbjct: 183 AIFNVSDPEEVAKQWSALL----QAEVVSEGDTYTINIGNKQLVFTAGVENRLFKLIFHG 238 Query: 237 ATDAPGLAGRRIRFGQAD 254 A L +IRF D Sbjct: 239 AKQPEKLQLGQIRFDLLD 256 >C2F652.1 C2F652_LACRE Rhodanese domain protein Length = 261 Score = 166 bits (421), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 92/258 (35%), Positives = 146/258 (56%), Gaps = 9/258 (3%) Query: 2 SSLQWDHTVHYINDLEQAVQTFTDNGLAAFPGGSHKLWGTHNALSYFGLNYVEFLAVENR 61 +L WDHT+ + +L+ A+ F GLA PGG H+ WGT NAL YFGLNY+E L + N+ Sbjct: 6 ETLNWDHTMVDVTNLQDAIDYFNSKGLAFTPGGHHEYWGTKNALDYFGLNYIELLTIANK 65 Query: 62 ELAESADTA-NLVVKDAVK-LLPEHEGFSRVAIRTDDIEQTAALLQQQGLKLSPIMDGRR 119 E A++ N + DAV+ + + +AI+TD+IE+T L+ GL + I DG+R Sbjct: 66 EKAKAFPYENNSAIHDAVEDYFAGIQRITTIAIQTDNIEETHQRLEGLGLNVGDITDGKR 125 Query: 120 LNKQGQWIEWRMLTIGGHF-QGLVYPFVIQWKGTDGQRQQELRTAGIIRPHPAGAVEVRA 178 ++ G+ I+W++ + L PF IQW D QR+++L+ G+I+ HPAG + V+ Sbjct: 126 VDPAGKLIQWKIFYVNDELVNHLPAPFFIQWGNNDDQRREDLKKQGLIKFHPAGDLFVKQ 185 Query: 179 AVFSVTDPAATAAHWSQVIGLPFQNHRSAADGSVSLAIGNKAFVFRQGNGEQMQELLL-- 236 A+F+V+DP A WS ++ Q + + ++ IG+K VF+ G ++ +L+ Sbjct: 186 AIFNVSDPEEAAKQWSALL----QAEVVSEGDTYTINIGDKQLVFKAGVENRLFKLIFHG 241 Query: 237 ATDAPGLAGRRIRFGQAD 254 A L +IRF D Sbjct: 242 AKQPEKLQLGQIRFDLLD 259 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.135 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,593,917,653 Number of extensions: 64418054 Number of successful extensions: 146923 Number of sequences better than 1.0e-05: 132 Number of HSP's gapped: 146747 Number of HSP's successfully gapped: 132 Length of query: 260 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 127 Effective length of database: 1,807,701,208 Effective search space: 229578053416 Effective search space used: 229578053416 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)