BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0397.0005.p (270 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q5NQR9.1 Q5NQR9_ZYMMO Pyrroline-5-carboxylate reductase EC=1.5.1.2 317 5e-85 B5XP17.1 B5XP17_KLEP3 Putative pyrroline-5-carboxylate reductase 317 1e-84 Q9FDK7.1 Q9FDK7_ZYMMO Pyrroline-5-carboxylate reductase 316 1e-84 B3XMZ7.1 B3XMZ7_LACRE Pyrroline-5-carboxylate reductase EC=1.5.1.2 256 1e-66 B2G516.1 B2G516_LACRJ Pyrroline-5-carboxylate reductase 254 4e-66 >Q5NQR9.1 Q5NQR9_ZYMMO Pyrroline-5-carboxylate reductase EC=1.5.1.2 Length = 271 Score = 317 bits (813), Expect = 5e-85, Method: Compositional matrix adjust. Identities = 156/265 (58%), Positives = 194/265 (73%), Gaps = 3/265 (1%) Query: 6 IHFIGGGQMAEAIIRAVIGKGAYKAEQISVADVNEERNLYLKNTYGIHAAVSQAAE---L 62 IHFIG GQM+EA+IRA+I A I+ D+ ER L + YGI L Sbjct: 4 IHFIGSGQMSEAMIRAIIKHKTASASDITAYDIRPEREKELHDNYGITTFAKPKKNIETL 63 Query: 63 GEAELIIIAVRPQDDLAAVGRLIAEHAAGQAAVLSIVAGVSIARLGGYAGAERPIIRVIP 122 +A++++I VRPQDDL AV IA++A +A ++SIVAGV++A+ G ER I R+IP Sbjct: 64 QKADIVVIGVRPQDDLKAVTADIAQNAKEKALIVSIVAGVTLAQFEDLLGKERAIARIIP 123 Query: 123 NTLTDTGLGYSGAALNGFADKAQVDDFLNAFGKVLYLEEAQIDIFTGYGVAGPNYVYYFI 182 NTLTDTGLGYSG +N ADKA + F++ FGK L+LEE+ IDIFTG+GV GPNYVYYFI Sbjct: 124 NTLTDTGLGYSGVVINNQADKALTEKFVSGFGKCLFLEESLIDIFTGFGVCGPNYVYYFI 183 Query: 183 ESLADAGVLAGLPREQAWQVALENVEGAVAMLRHSGKHPRQLLDINNSPGGVGIHALYEL 242 E+L DAGVLAGLPR+ AW+ +EN+ G+V ML SGKHPRQLLDINNSP GVG+++L+ L Sbjct: 184 EALVDAGVLAGLPRDIAWETTMENLVGSVKMLEISGKHPRQLLDINNSPAGVGMNSLFAL 243 Query: 243 NNSDFAAGLQRSVKAAVKRTTELGQ 267 N SDF AGLQ+SV AAVKRTTELGQ Sbjct: 244 NQSDFTAGLQKSVLAAVKRTTELGQ 268 >B5XP17.1 B5XP17_KLEP3 Putative pyrroline-5-carboxylate reductase Length = 262 Score = 317 bits (811), Expect = 1e-84, Method: Compositional matrix adjust. Identities = 159/263 (60%), Positives = 189/263 (71%), Gaps = 4/263 (1%) Query: 5 RIHFIGGGQMAEAIIRAVIGKGAYKAEQISVADVNEERNLYLKNTYGIHAAVSQAAELGE 64 ++HFIG GQM EAIIRA + G +A+ IS+ D++ R L + Y + S L E Sbjct: 3 KVHFIGAGQMTEAIIRASLSNGTLRADAISLEDIDSARIEALHSRYRL----SGDGGLRE 58 Query: 65 AELIIIAVRPQDDLAAVGRLIAEHAAGQAAVLSIVAGVSIARLGGYAGAERPIIRVIPNT 124 A L+++ +RPQDDLAAV + G V+S++AGV++ +L G PI R IPNT Sbjct: 59 ASLLVLGIRPQDDLAAVASRVRAQLNGSTTVVSLIAGVTLEKLASLFGETTPIARTIPNT 118 Query: 125 LTDTGLGYSGAALNGFADKAQVDDFLNAFGKVLYLEEAQIDIFTGYGVAGPNYVYYFIES 184 LTDTG GYSG LN A A+++ FL FGKVLYL E ID+FTG+GVAGPNY+YYFIES Sbjct: 119 LTDTGFGYSGVTLNAHASAAEIEPFLRGFGKVLYLPERLIDVFTGFGVAGPNYIYYFIES 178 Query: 185 LADAGVLAGLPREQAWQVALENVEGAVAMLRHSGKHPRQLLDINNSPGGVGIHALYELNN 244 L DAGVLAGL R QA +V LEN+ GAV MLR S KHPRQLLDINNSP GVGIHALYELNN Sbjct: 179 LTDAGVLAGLSRPQATEVVLENLLGAVEMLRQSQKHPRQLLDINNSPAGVGIHALYELNN 238 Query: 245 SDFAAGLQRSVKAAVKRTTELGQ 267 SDFAAGLQRSV AAV+RT ELGQ Sbjct: 239 SDFAAGLQRSVLAAVRRTRELGQ 261 >Q9FDK7.1 Q9FDK7_ZYMMO Pyrroline-5-carboxylate reductase Length = 271 Score = 316 bits (810), Expect = 1e-84, Method: Compositional matrix adjust. Identities = 156/265 (58%), Positives = 193/265 (72%), Gaps = 3/265 (1%) Query: 6 IHFIGGGQMAEAIIRAVIGKGAYKAEQISVADVNEERNLYLKNTYGIHAAVSQAAE---L 62 IHFIG GQM+EA+IRA+I A I+ D+ ER L + YGI L Sbjct: 4 IHFIGSGQMSEAMIRAIIKHKTASASDITAYDIRPEREKELHDNYGITTFAKPKKNIETL 63 Query: 63 GEAELIIIAVRPQDDLAAVGRLIAEHAAGQAAVLSIVAGVSIARLGGYAGAERPIIRVIP 122 +A++++I VRPQDDL AV IA++A +A ++SIVAGV++A+ G ER I R+IP Sbjct: 64 QKADIVVIGVRPQDDLKAVTADIAQNAKEKALIVSIVAGVTLAQFEDLLGKERAIARIIP 123 Query: 123 NTLTDTGLGYSGAALNGFADKAQVDDFLNAFGKVLYLEEAQIDIFTGYGVAGPNYVYYFI 182 NTLTDTGLGYSG +N ADKA + F++ FGK L+LEE+ IDIFTG+GV GPNYVYYFI Sbjct: 124 NTLTDTGLGYSGVVINNQADKALTEKFVSGFGKCLFLEESLIDIFTGFGVCGPNYVYYFI 183 Query: 183 ESLADAGVLAGLPREQAWQVALENVEGAVAMLRHSGKHPRQLLDINNSPGGVGIHALYEL 242 E+L DAGVLAGLPR+ AW+ +EN+ G+V ML SGKHPRQLLDINNSP GVG+++L+ L Sbjct: 184 EALVDAGVLAGLPRDIAWETTMENLVGSVKMLEISGKHPRQLLDINNSPAGVGMNSLFAL 243 Query: 243 NNSDFAAGLQRSVKAAVKRTTELGQ 267 N SDF AGLQ SV AAVKRTTELGQ Sbjct: 244 NQSDFTAGLQNSVLAAVKRTTELGQ 268 >B3XMZ7.1 B3XMZ7_LACRE Pyrroline-5-carboxylate reductase EC=1.5.1.2 Length = 270 Score = 256 bits (654), Expect = 1e-66, Method: Compositional matrix adjust. Identities = 134/265 (50%), Positives = 184/265 (69%), Gaps = 5/265 (1%) Query: 5 RIHFIGGGQMAEAIIRAVIGKGAYKAEQISVADVNEERNLYLKNTYGIHAAVS-QAAELG 63 +I+ GGGQM EA++RA + KG E V DV+ +R YL +TY I A+ + Sbjct: 4 KIYSFGGGQMVEAMLRAALKKGVITPETTGVTDVSIDRVNYLTDTYHIQASQRPDWTAVE 63 Query: 64 EAELIIIAVRPQDDLAAVGR-LIAEHAAGQAAVLSIVAGVSIARLGGYAGAERPIIRVIP 122 A+L+I+ VRPQD+ + + L+A H Q VLSI+AGV++A+L I R+IP Sbjct: 64 NADLVILGVRPQDNWQEIMKELVAHHY--QKDVLSIIAGVTVAQLSSQEDL-FAITRIIP 120 Query: 123 NTLTDTGLGYSGAALNGFADKAQVDDFLNAFGKVLYLEEAQIDIFTGYGVAGPNYVYYFI 182 NTLTD +GYSG N A++ ++DFLN+FGKV Y++E+ +D+FTGYGVAGPNY+Y F+ Sbjct: 121 NTLTDVAMGYSGVVKNDRANQTVIEDFLNSFGKVDYIDESLLDVFTGYGVAGPNYIYNFL 180 Query: 183 ESLADAGVLAGLPREQAWQVALENVEGAVAMLRHSGKHPRQLLDINNSPGGVGIHALYEL 242 S +AGVLAG+PR+QA ++ALEN+ A A + SGKHP +LLDINNS GGVGI A +EL Sbjct: 181 ISFTNAGVLAGMPRQQANKLALENLRAAAAFVEQSGKHPAELLDINNSAGGVGITAQHEL 240 Query: 243 NNSDFAAGLQRSVKAAVKRTTELGQ 267 + S F+AG++ +V AAVKRT ELG+ Sbjct: 241 DKSSFSAGIENAVLAAVKRTKELGK 265 >B2G516.1 B2G516_LACRJ Pyrroline-5-carboxylate reductase Length = 270 Score = 254 bits (650), Expect = 4e-66, Method: Compositional matrix adjust. Identities = 131/265 (49%), Positives = 183/265 (69%), Gaps = 5/265 (1%) Query: 5 RIHFIGGGQMAEAIIRAVIGKGAYKAEQISVADVNEERNLYLKNTYGIHAAVS-QAAELG 63 +I+ GGGQM EA++RA + K E + D++ +R YL +TY I A+ + Sbjct: 4 KIYSFGGGQMVEAMLRAALKKKVITPETTGITDISSDRVNYLTDTYHIQASQQPDWTAVE 63 Query: 64 EAELIIIAVRPQDDLAAVGR-LIAEHAAGQAAVLSIVAGVSIARLGGYAGAERPIIRVIP 122 A+L+I+ VRPQD+ + + L+A H Q VLSI+AGV++A+L I R+IP Sbjct: 64 NADLVILGVRPQDNWQEIMKELVAHHY--QKDVLSIIAGVTVAQLSSQEDL-FAITRIIP 120 Query: 123 NTLTDTGLGYSGAALNGFADKAQVDDFLNAFGKVLYLEEAQIDIFTGYGVAGPNYVYYFI 182 NTLTD +GYSG N A++ ++DFLN+FGKV Y++E+ +D+FTGYGVAGPNY+Y F+ Sbjct: 121 NTLTDVAMGYSGVVKNNRANQTVIEDFLNSFGKVDYIDESLLDVFTGYGVAGPNYIYNFL 180 Query: 183 ESLADAGVLAGLPREQAWQVALENVEGAVAMLRHSGKHPRQLLDINNSPGGVGIHALYEL 242 S +AGVLAG+PR+QA ++ALEN+ A A + SGKHP +LLDINNS GGVGI A +EL Sbjct: 181 ISFTNAGVLAGMPRQQANKLALENLRAAAAFVEQSGKHPAELLDINNSAGGVGITAQHEL 240 Query: 243 NNSDFAAGLQRSVKAAVKRTTELGQ 267 + S F+AG++ +V AAVKRT ELG+ Sbjct: 241 DKSTFSAGIENAVLAAVKRTKELGK 265 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.136 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,563,396,264 Number of extensions: 66027370 Number of successful extensions: 190320 Number of sequences better than 1.0e-05: 1905 Number of HSP's gapped: 186838 Number of HSP's successfully gapped: 1905 Length of query: 270 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 137 Effective length of database: 1,807,701,208 Effective search space: 247655065496 Effective search space used: 247655065496 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)