BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0392.0005.p (542 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KKS3.1 B3KKS3_9BACI Peptide chain release factor 3 972 0.0 B1DF52.1 B1DF52_9BACL Peptide chain release factor 3 952 0.0 C0ZBM8.1 C0ZBM8_BREBN Peptide chain release factor 3 709 0.0 A3DC87.1 A3DC87_CLOTH Bacterial peptide chain release factor 3 (... 693 0.0 C1PQK6.1 C1PQK6_ALIAC Bacterial peptide chain release factor 3 (... 682 0.0 >B3KKS3.1 B3KKS3_9BACI Peptide chain release factor 3 Length = 526 Score = 972 bits (2512), Expect = 0.0, Method: Compositional matrix adjust. Identities = 467/528 (88%), Positives = 502/528 (95%), Gaps = 2/528 (0%) Query: 15 MNKALDQKLQQEVDKRRTFAIISHPDAGKTTLTEKLLLFGGAIRLAGSVKARKASKHATS 74 M K +D+ LQQEVDKRRTFAIISHPDAGKTTLTEKLLLFGGAIRLAGSVKARKASKHATS Sbjct: 1 MAKLIDE-LQQEVDKRRTFAIISHPDAGKTTLTEKLLLFGGAIRLAGSVKARKASKHATS 59 Query: 75 DWMEIEKQRGISVTSSVMQFDYMGHRVNILDTPGHQDFSEDTYRTLTAADSAVMLIDVAK 134 DWMEIEKQRGISVTSSVMQFDY+GHR+NILDTPGHQDFSEDTYRTLTAADSAVMLIDVAK Sbjct: 60 DWMEIEKQRGISVTSSVMQFDYLGHRINILDTPGHQDFSEDTYRTLTAADSAVMLIDVAK 119 Query: 135 GVEAQTIKLFQVCAKRGIPIFTFINKLDREGQSPFELMEELENVLGIRSVPMNWPIGGGR 194 GVEAQTIKLFQVCAKRGIPIFTFINKLDREG+SPF+LMEELE VLGIRSVPMNWPIG GR Sbjct: 120 GVEAQTIKLFQVCAKRGIPIFTFINKLDREGRSPFDLMEELEQVLGIRSVPMNWPIGSGR 179 Query: 195 ELCGVYDRMKNQVELFQGDDHSVIKVQKVESYNDPVIREMAGEYLHDQLCQDLELLDVAG 254 +LCGVYDR+KNQVELFQGDDHS IKVQKVE Y D +IREMAG+YLHDQLCQDLELLDVAG Sbjct: 180 DLCGVYDRVKNQVELFQGDDHSTIKVQKVEGYEDSLIREMAGDYLHDQLCQDLELLDVAG 239 Query: 255 DAFDYEKVLRGELTPVFFGSAINNFGVQTFLDNFLELAPKPEPRRSNAGDVEPTNEKFTG 314 D FD EKV++G+LTPVFFGSAINNFGVQTFL+NFL+LAPKPEPRRS G VEPT+EKF+G Sbjct: 240 DPFDLEKVMQGQLTPVFFGSAINNFGVQTFLENFLQLAPKPEPRRSTEGVVEPTDEKFSG 299 Query: 315 YVFKIQANMNPAHRDRIAFLRIVSGKFERGMSVKHVRAGKDIKLSQPQQFLAQDRDIVEE 374 YVFKIQANMNPAHRDRIAFLRIVSGKFERGMSVKHVR GK+IKL+QPQQFLAQDRDIV+E Sbjct: 300 YVFKIQANMNPAHRDRIAFLRIVSGKFERGMSVKHVRVGKEIKLAQPQQFLAQDRDIVQE 359 Query: 375 AFPGDIIGLFDPGIFRIGDTLSQAGDIEFDELPTFSPEIFSKVSIKNALKSKQFQKGIDQ 434 A+PGDIIGLFDPGIFRIGD+LSQ ++ FDELPTFSPEIF+KV++KNALK KQ+QKGIDQ Sbjct: 360 AYPGDIIGLFDPGIFRIGDSLSQGREVVFDELPTFSPEIFAKVTVKNALKHKQYQKGIDQ 419 Query: 435 LTEEGMIQVFRTVNFDDILLGVVGQLQFEVFEYRMKGEYGVDVQLQRMSYQFARWIVDEN 494 LTEEG IQVFRTV+FD+ +LGVVGQLQFEVFEYRMKGEYGVDVQLQRM YQFARW+VD+N Sbjct: 420 LTEEGTIQVFRTVSFDETILGVVGQLQFEVFEYRMKGEYGVDVQLQRMPYQFARWLVDDN 479 Query: 495 KPDPSKFRINSTLVTDKKGNYVVLFENEYAMRTAMEKNPTAKFLETAP 542 DPSKFRINSTLV DKK NYVVLFENEYAMRTA++KNPTAKFLETAP Sbjct: 480 I-DPSKFRINSTLVKDKKDNYVVLFENEYAMRTAIDKNPTAKFLETAP 526 >B1DF52.1 B1DF52_9BACL Peptide chain release factor 3 Length = 529 Score = 952 bits (2460), Expect = 0.0, Method: Compositional matrix adjust. Identities = 455/530 (85%), Positives = 492/530 (92%), Gaps = 3/530 (0%) Query: 15 MNKALDQKLQQEVDKRRTFAIISHPDAGKTTLTEKLLLFGGAIRLAGSVKARKASKHATS 74 M++ L ++ QEVDKRRTFAIISHPDAGKTTLTEKLLLFGGAIRLAGSVKARKAS+HATS Sbjct: 1 MSQTLSNEITQEVDKRRTFAIISHPDAGKTTLTEKLLLFGGAIRLAGSVKARKASRHATS 60 Query: 75 DWMEIEKQRGISVTSSVMQFDYMGHRVNILDTPGHQDFSEDTYRTLTAADSAVMLIDVAK 134 DWMEIEKQRGISVTSSVMQFDY+GHRVNILDTPGHQDFSEDTYRTLTAADSAVMLIDVAK Sbjct: 61 DWMEIEKQRGISVTSSVMQFDYLGHRVNILDTPGHQDFSEDTYRTLTAADSAVMLIDVAK 120 Query: 135 GVEAQTIKLFQVCAKRGIPIFTFINKLDREGQSPFELMEELENVLGIRSVPMNWPIGGGR 194 GVEAQTIKLFQVC KRGIPIFTFINKLDREGQSPFELMEELE VLGIRSVPMNWPIG GR Sbjct: 121 GVEAQTIKLFQVCRKRGIPIFTFINKLDREGQSPFELMEELERVLGIRSVPMNWPIGMGR 180 Query: 195 ELCGVYDRMKNQVELFQGDDHSVIKVQKVESYNDPVIREMAGEYLHDQLCQDLELLDVAG 254 ELCGVYDRMKNQVELFQG+DHS I+V+KVE YNDP+IREMAG+YLHDQL QDLELLDVAG Sbjct: 181 ELCGVYDRMKNQVELFQGNDHSTIEVRKVEDYNDPIIREMAGDYLHDQLIQDLELLDVAG 240 Query: 255 DAFDYEKVLRGELTPVFFGSAINNFGVQTFLDNFLELAPKPEPRRSNAGDVEPTNEKFTG 314 D FD+EKV GELTP+FFGSA+NNFGVQTFL+NFLELAPKP PR S G V+PTNEKF+G Sbjct: 241 DPFDFEKVRAGELTPIFFGSAVNNFGVQTFLENFLELAPKPAPRMSTNGSVDPTNEKFSG 300 Query: 315 YVFKIQANMNPAHRDRIAFLRIVSGKFERGMSVKHVRAGKDIKLSQPQQFLAQDRDIVEE 374 YVFKIQANMNPAHRDRIAFLRI SG+FERGMSV+H+RAGKDIKLSQPQQFLAQDRDIVE Sbjct: 301 YVFKIQANMNPAHRDRIAFLRICSGRFERGMSVRHIRAGKDIKLSQPQQFLAQDRDIVET 360 Query: 375 AFPGDIIGLFDPGIFRIGDTLSQAGDIEFDELPTFSPEIFSKVSIKNALKSKQFQKGIDQ 434 A+PGDIIGLFDPGIFRIGD+LSQ ++ FDELPTFSPEIF+KV++KNALK KQ+QKGIDQ Sbjct: 361 AYPGDIIGLFDPGIFRIGDSLSQGSEVVFDELPTFSPEIFAKVTVKNALKHKQYQKGIDQ 420 Query: 435 LTEEGMIQVFRTVN--FDDILLGVVGQLQFEVFEYRMKGEYGVDVQLQRMSYQFARWIVD 492 LTEEG IQVF + + D+ +LGVVG LQFEVFEYRMKGEYGVD+QL RM +QFARWI+D Sbjct: 421 LTEEGTIQVFYSASGFADETILGVVGHLQFEVFEYRMKGEYGVDIQLHRMPFQFARWIID 480 Query: 493 ENKPDPSKFRINSTLVTDKKGNYVVLFENEYAMRTAMEKNPTAKFLETAP 542 +K DPSKFRINSTLV DKKGNYVVLFENEYAMRTAM+KNPT KFLETAP Sbjct: 481 -DKIDPSKFRINSTLVKDKKGNYVVLFENEYAMRTAMDKNPTMKFLETAP 529 >C0ZBM8.1 C0ZBM8_BREBN Peptide chain release factor 3 Length = 532 Score = 709 bits (1831), Expect = 0.0, Method: Compositional matrix adjust. Identities = 343/521 (65%), Positives = 416/521 (79%), Gaps = 4/521 (0%) Query: 22 KLQQEVDKRRTFAIISHPDAGKTTLTEKLLLFGGAIRLAGSVKARKASKHATSDWMEIEK 81 + + E+ KRRTFAIISHPDAGKTT+TEKLL +GGAIR AG VK RK SKHATSDWMEIEK Sbjct: 7 QWENEIAKRRTFAIISHPDAGKTTMTEKLLYYGGAIREAGVVKGRKNSKHATSDWMEIEK 66 Query: 82 QRGISVTSSVMQFDYMGHRVNILDTPGHQDFSEDTYRTLTAADSAVMLIDVAKGVEAQTI 141 +RGISVTSS M F+Y GH +NILDTPGHQDFSEDTYRTLTAAD+AVM+IDVAKGVEAQTI Sbjct: 67 KRGISVTSSAMDFEYNGHHINILDTPGHQDFSEDTYRTLTAADAAVMIIDVAKGVEAQTI 126 Query: 142 KLFQVCAKRGIPIFTFINKLDREGQSPFELMEELENVLGIRSVPMNWPIGGGRELCGVYD 201 KLF+VC RGIPIFTF+NKLDR G+ PFEL+EE+E VLGIRS PMNWPIG G GV+D Sbjct: 127 KLFKVCRMRGIPIFTFVNKLDRHGKDPFELLEEIERVLGIRSYPMNWPIGMGSYFSGVFD 186 Query: 202 RMKNQVELFQGD-DHSVIKVQKVESYNDPVIREMAGEYLHDQLCQDLELLDVAGDAFDYE 260 RMK++VEL+Q + H I VE DP+I + G+ L QL ++ LLDVAGD +D E Sbjct: 187 RMKSRVELYQNNTQHEEISFFPVEGAEDPLIGDRVGQELWQQLQDEISLLDVAGDEYDPE 246 Query: 261 KVLRGELTPVFFGSAINNFGVQTFLDNFLELAPKPEPRRSNAGDVEPTNEKFTGYVFKIQ 320 + +G+LTPVFFGSAINNFGVQTFLDNFL++AP P ++S G ++P +F+G++FKIQ Sbjct: 247 LINKGQLTPVFFGSAINNFGVQTFLDNFLQMAPAPSAKKSTEGLIDPYTNEFSGFIFKIQ 306 Query: 321 ANMNPAHRDRIAFLRIVSGKFERGMSVKHVRAGKDIKLSQPQQFLAQDRDIVEEAFPGDI 380 ANMNPAHRDRIAFLRI SGKFERGM+VKHVR GKDIKL+QP QF+AQDR+IV+E+F GD+ Sbjct: 307 ANMNPAHRDRIAFLRICSGKFERGMAVKHVRLGKDIKLAQPVQFMAQDREIVDESFAGDV 366 Query: 381 IGLFDPGIFRIGDTLSQAGDIEFDELPTFSPEIFSKVSIKNALKSKQFQKGIDQLTEEGM 440 IGLFDPGIF+IGDTL E++E+P FSPE FSKV +K+A+K KQFQKGI QLTEEG Sbjct: 367 IGLFDPGIFQIGDTLCVGKSFEYEEMPHFSPEFFSKVMVKDAMKHKQFQKGIMQLTEEGT 426 Query: 441 IQVFRTVNFDDILLGVVGQLQFEVFEYRMKGEYGVDVQLQRMSYQFARWIVDENKPDPSK 500 +Q+FRT ++++LGVVG LQFEV EYR+K EYGVD+ L RM YQ ARW+ E K D Sbjct: 427 VQLFRTHPMEELILGVVGVLQFEVLEYRLKAEYGVDIMLTRMPYQIARWLEGE-KADLDA 485 Query: 501 FRINSTLVTDKKGNYVVLFENEYAMRTAMEKNPTAKFLETA 541 + + V+D+ G V+LFE+EY +R AM+K KF +++ Sbjct: 486 --VKNRTVSDRYGRPVMLFESEYQLRVAMDKYSNIKFHDSS 524 >A3DC87.1 A3DC87_CLOTH Bacterial peptide chain release factor 3 (BRF-3) Length = 533 Score = 693 bits (1788), Expect = 0.0, Method: Compositional matrix adjust. Identities = 335/529 (63%), Positives = 427/529 (80%), Gaps = 7/529 (1%) Query: 19 LDQKLQQEVDKRRTFAIISHPDAGKTTLTEKLLLFGGAIRLAGSVKARKASKHATSDWME 78 + K+ EV++RRTFAIISHPDAGKTTLTEKLLL+GGAIRLAGSVKARKA+K+ATSDWME Sbjct: 1 MQNKISYEVERRRTFAIISHPDAGKTTLTEKLLLYGGAIRLAGSVKARKANKYATSDWME 60 Query: 79 IEKQRGISVTSSVMQFDYMGHRVNILDTPGHQDFSEDTYRTLTAADSAVMLIDVAKGVEA 138 IEKQRGISVTSSV+QF+Y + +NILDTPGHQDFSEDTYRTL AADSAVMLID AKGVE Sbjct: 61 IEKQRGISVTSSVLQFEYNNYCINILDTPGHQDFSEDTYRTLMAADSAVMLIDGAKGVEE 120 Query: 139 QTIKLFQVCAKRGIPIFTFINKLDREGQSPFELMEELENVLGIRSVPMNWPIGGGRELCG 198 QTIKLF VC RGIPIFTF+NK+DR + PFELMEELENVLGIRS PMNWPIG + G Sbjct: 121 QTIKLFHVCKMRGIPIFTFVNKMDRASKDPFELMEELENVLGIRSYPMNWPIGTDGDFKG 180 Query: 199 VYDRMKNQVELFQGDDHSVIKVQKVE-SYNDPVIREMAGEYLHDQLCQDLELLDVAGDAF 257 VY+R +Q+ELF+G +H V ++ S +D + ++ G++ H +LC+D+ELLD+AGD F Sbjct: 181 VYNRKLSQIELFEGGNHGQTVVSSIKGSVDDKIFADLLGDHYHKKLCEDIELLDMAGDPF 240 Query: 258 DYEKVLRGELTPVFFGSAINNFGVQTFLDNFLELAPKPEPRRSNAGDVEPTNEKFTGYVF 317 D EK+L+GELTP+FFGSA+ NFGVQ FL+ FL+LAPKP + S+ G+V+P ++KFTG+VF Sbjct: 241 DKEKILKGELTPMFFGSAMTNFGVQPFLEEFLQLAPKPGIKMSSEGEVDPESDKFTGFVF 300 Query: 318 KIQANMNPAHRDRIAFLRIVSGKFERGMSVKHVRAGKDIKLSQPQQFLAQDRDIVEEAFP 377 KIQANMNPAHRDRIAF+RI SG+F RGM+V HV+ GK+++LSQPQQF+AQ+R IVEEA+ Sbjct: 301 KIQANMNPAHRDRIAFIRICSGRFTRGMTVYHVQQGKEVRLSQPQQFMAQERTIVEEAYA 360 Query: 378 GDIIGLFDPGIFRIGDTLSQA-GDIEFDELPTFSPEIFSKVSIKNALKSKQFQKGIDQLT 436 GDIIG+FDPGIF IGDTLS+ ++ F+ +P F E F+KV+ + +K KQF KGI+QL+ Sbjct: 361 GDIIGVFDPGIFHIGDTLSEGNSNLRFEGIPIFPAEHFAKVTPVDTMKRKQFIKGINQLS 420 Query: 437 EEGMIQVFRTVN--FDDILLGVVGQLQFEVFEYRMKGEYGVDVQLQRMSYQFARWIVDEN 494 EEG IQVF+ ++ F+ I++GVVG LQ EV EYR+K EYGVD+++Q++ ++ ARWIV + Sbjct: 421 EEGTIQVFKQIDIGFESIIVGVVGILQLEVLEYRLKHEYGVDLRIQKLPHRHARWIVSSD 480 Query: 495 KPDPSKFRINST--LVTDKKGNYVVLFENEYAMRTAMEKNPTAKFLETA 541 +P + ST +V D+ YV+LFENE+++R A EKNP+ K + A Sbjct: 481 T-EPRNLTLTSTTMIVHDQDERYVLLFENEWSIRWAEEKNPSLKLEDIA 528 >C1PQK6.1 C1PQK6_ALIAC Bacterial peptide chain release factor 3 (BRF-3) Length = 545 Score = 682 bits (1760), Expect = 0.0, Method: Compositional matrix adjust. Identities = 328/514 (63%), Positives = 406/514 (78%), Gaps = 3/514 (0%) Query: 30 RRTFAIISHPDAGKTTLTEKLLLFGGAIRLAGSVKARKASKHATSDWMEIEKQRGISVTS 89 RRTFAIISHPDAGKTT+TEKLLLFGGAIR AG+VK RKA +HATSDWMEIEKQRGISVTS Sbjct: 21 RRTFAIISHPDAGKTTMTEKLLLFGGAIREAGAVKGRKARRHATSDWMEIEKQRGISVTS 80 Query: 90 SVMQFDYMGHRVNILDTPGHQDFSEDTYRTLTAADSAVMLIDVAKGVEAQTIKLFQVCAK 149 +V+QFDYMG R+N+LDTPGH+DFSEDTYRTL AADSAVMLID AKGVE QTIKLF+VC Sbjct: 81 TVLQFDYMGCRLNLLDTPGHEDFSEDTYRTLAAADSAVMLIDAAKGVEPQTIKLFEVCRM 140 Query: 150 RGIPIFTFINKLDREGQSPFELMEELENVLGIRSVPMNWPIGGGRELCGVYDRMKNQVEL 209 RGIPIFTFINKLDR G+ PFELMEE+E VLGIRS P+ WPIG G + G+Y RM+ + E Sbjct: 141 RGIPIFTFINKLDRHGKDPFELMEEIERVLGIRSCPVTWPIGMGPDFQGIYHRMEGRFER 200 Query: 210 FQGDDHSVIKVQKVESYNDPVIREMAGEYLHDQLCQDLELLDVAGDAFDYEKVLRGELTP 269 F + VQ V NDP + E+ GE L +L D+ELLDVAGD FD + V +G+LTP Sbjct: 201 FTSRTEEMTSVQ-VPDLNDPALAEVLGEALWQKLKDDIELLDVAGDPFDADLVRQGKLTP 259 Query: 270 VFFGSAINNFGVQTFLDNFLELAPKPEPRRSNAGDVEPTNEKFTGYVFKIQANMNPAHRD 329 ++FGSAI NFGV +FL++F++LAP P PR +NAG V P + FTG+VFKIQANMNPAHRD Sbjct: 260 MYFGSAIANFGVASFLESFIQLAPAPGPRETNAGVVRPDDPVFTGFVFKIQANMNPAHRD 319 Query: 330 RIAFLRIVSGKFERGMSVKHVRAGKDIKLSQPQQFLAQDRDIVEEAFPGDIIGLFDPGIF 389 R+AF+RI SGKF RGM+V HVR GK + L+QPQQF Q+R+ V+EA+ GDIIG+ DPG+F Sbjct: 320 RVAFVRICSGKFARGMTVTHVRTGKRLALTQPQQFFGQERETVDEAYAGDIIGIHDPGLF 379 Query: 390 RIGDTLSQAGDIEFDELPTFSPEIFSKVSIKNALKSKQFQKGIDQLTEEGMIQVFRTVN- 448 RIGDTL++ G +EF LP+FSPE F++VS+ NA+K KQF KGI +L+EEG +QVFR N Sbjct: 380 RIGDTLAERGTLEFPPLPSFSPEHFARVSVPNAMKYKQFHKGIAELSEEGAVQVFRQANR 439 Query: 449 FDDILLGVVGQLQFEVFEYRMKGEYGVDVQLQRMSYQFARWIVDENKPDPSKFRINSTLV 508 +D++LG VGQLQF+VF YRMK EYGVDV++ R+ YQFARW+ + + +F +TLV Sbjct: 440 TEDLILGAVGQLQFDVFVYRMKHEYGVDVEMIRLPYQFARWLASDEPVEHLQFDRYATLV 499 Query: 509 T-DKKGNYVVLFENEYAMRTAMEKNPTAKFLETA 541 D+ G V+LF+ E+AM+ ++KNP + +T+ Sbjct: 500 VRDRDGRTVLLFQTEFAMQWLLDKNPNVRLHKTS 533 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.137 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,164,384,263 Number of extensions: 132983302 Number of successful extensions: 384860 Number of sequences better than 1.0e-05: 9943 Number of HSP's gapped: 369439 Number of HSP's successfully gapped: 9958 Length of query: 542 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 401 Effective length of database: 1,732,333,088 Effective search space: 694665568288 Effective search space used: 694665568288 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)