BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0391.0036.p (378 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C1P8Y1.1 C1P8Y1_BACCO Polysaccharide deacetylase 200 2e-49 B4AFR6.1 B4AFR6_BACPU Polysaccharide deacetylase, Carbohydrate E... 188 9e-46 A8FBI2.1 A8FBI2_BACP2 Possible polysaccharide deacetylase EC=3.1... 186 3e-45 O07596.1 O07596_BACSU Putative uncharacterized protein yheN EC=3... 186 4e-45 Q2BA98.1 Q2BA98_9BACI Polysaccharide deacetylase, putative 184 1e-44 >C1P8Y1.1 C1P8Y1_BACCO Polysaccharide deacetylase Length = 311 Score = 200 bits (508), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 92/201 (45%), Positives = 131/201 (65%), Gaps = 2/201 (0%) Query: 178 LYLTFDDGPSATTPELLDILNKYGVKATFFMLGPNMNRYPSQVKRIAEEGNGLGLHGMTH 237 +YLTFDDGPS+ +LLD+L Y +KATFFMLGP M +P+ VKR+ ++GNGL LHG+TH Sbjct: 100 IYLTFDDGPSSAANQLLDLLETYQMKATFFMLGPRMKEHPAVVKRMLKDGNGLALHGITH 159 Query: 238 RKEKFYASASAALAEMNGDNDVLRKITGTGTTLIRPPYGSKPYFTKAFRDKVLGQGYHLW 297 + Y+S SA L EM D +L + G + ++R PYGS PY T RD + G+H+W Sbjct: 160 KARTIYSSTSAPLNEMAEDQRILENVAGVKSYMVRLPYGSIPYLTDEMRDLLDKNGFHIW 219 Query: 298 DWNVDSDDWKYKEDSVTIYNTVMGQVHKLQKSKTNPVILMHDQKATLKVLPRLLESLKKE 357 DWNVDS DW+ K Y + Q+ + +++ PVIL+HD+ T+K LP+LL+ +KK+ Sbjct: 220 DWNVDSSDWELKNGRYVQY--TIQQIRQKKQAGETPVILLHDKPETVKYLPKLLKFIKKQ 277 Query: 358 GYTFGIITKDMEPVNFWKDKR 378 GY ++T + PV F + R Sbjct: 278 GYKTKVLTNETPPVTFPCEGR 298 >B4AFR6.1 B4AFR6_BACPU Polysaccharide deacetylase, Carbohydrate Esterase Family 4 Length = 284 Score = 188 bits (477), Expect = 9e-46, Method: Compositional matrix adjust. Identities = 90/197 (45%), Positives = 126/197 (63%) Query: 176 KTLYLTFDDGPSATTPELLDILNKYGVKATFFMLGPNMNRYPSQVKRIAEEGNGLGLHGM 235 +T++LTFDDGPSAT+ +LLD+L + VKATFFMLGP + +P+ VKR+ +EG+ LGLHG+ Sbjct: 87 QTIFLTFDDGPSATSNQLLDVLKAHQVKATFFMLGPQIKEHPAAVKRLHQEGHQLGLHGI 146 Query: 236 THRKEKFYASASAALAEMNGDNDVLRKITGTGTTLIRPPYGSKPYFTKAFRDKVLGQGYH 295 TH ++FY + + + EM D +L +TG T L+R PYGS P T + ++ +G+ Sbjct: 147 THDVKRFYQKSDSPVNEMKEDQRILASVTGEYTHLVRTPYGSAPNLTDQQKARLKQKGFI 206 Query: 296 LWDWNVDSDDWKYKEDSVTIYNTVMGQVHKLQKSKTNPVILMHDQKATLKVLPRLLESLK 355 WDW +DS DWKYK QV + + K +ILMHDQ AT K L LL LK Sbjct: 207 YWDWTIDSLDWKYKSSKYVPEVLNQLQVLETKHPKEPKIILMHDQPATAKYLNSLLTQLK 266 Query: 356 KEGYTFGIITKDMEPVN 372 +GYTF ++ + MEP+ Sbjct: 267 AKGYTFEVLDETMEPLQ 283 >A8FBI2.1 A8FBI2_BACP2 Possible polysaccharide deacetylase EC=3.1.-.- Length = 284 Score = 186 bits (472), Expect = 3e-45, Method: Compositional matrix adjust. Identities = 89/197 (45%), Positives = 126/197 (63%) Query: 176 KTLYLTFDDGPSATTPELLDILNKYGVKATFFMLGPNMNRYPSQVKRIAEEGNGLGLHGM 235 +T++LTFDDGPSAT+ +LLD+L + VKATFFMLGP + +P+ VKR+ +EG+ LGLHG+ Sbjct: 87 QTIFLTFDDGPSATSNQLLDVLKAHQVKATFFMLGPQIKEHPAAVKRLHQEGHQLGLHGI 146 Query: 236 THRKEKFYASASAALAEMNGDNDVLRKITGTGTTLIRPPYGSKPYFTKAFRDKVLGQGYH 295 TH ++FY + + + EM D +L +TG T L+R PYGS P T + ++ +G+ Sbjct: 147 THDVKRFYQKSDSPVNEMKEDQRILASVTGEYTHLVRTPYGSAPNLTDQQKARLKQKGFI 206 Query: 296 LWDWNVDSDDWKYKEDSVTIYNTVMGQVHKLQKSKTNPVILMHDQKATLKVLPRLLESLK 355 WDW +DS DWKYK QV + + K +ILMHDQ AT K L LL LK Sbjct: 207 YWDWTIDSLDWKYKSSKYVPEVLNQLQVLETKHPKEPKIILMHDQPATAKYLNSLLTQLK 266 Query: 356 KEGYTFGIITKDMEPVN 372 +GYTF ++ + M+P+ Sbjct: 267 AKGYTFEVLDETMKPLQ 283 >O07596.1 O07596_BACSU Putative uncharacterized protein yheN EC=3.5.1.- Length = 282 Score = 186 bits (471), Expect = 4e-45, Method: Compositional matrix adjust. Identities = 93/199 (46%), Positives = 123/199 (61%), Gaps = 4/199 (2%) Query: 176 KTLYLTFDDGPSATTPELLDILNKYGVKATFFMLGPNMNRYPSQVKRIAEEGNGLGLHGM 235 KT+YLTFDDGPSA T LLD+L VKATFFML P MN + VKR +EG+ LGLHG+ Sbjct: 85 KTVYLTFDDGPSAVTTRLLDVLKSADVKATFFMLSPRMNEFKQAVKRAEKEGHALGLHGV 144 Query: 236 THRKEKFYASASAALAEMNGDNDVLRKITGTGTTLIRPPYGSKPYFTKAFRDKVLGQGYH 295 TH FY + ++ L EM D L+ ITG T L+R PYGSKP T + + G+ Sbjct: 145 THNNRLFYQTPTSPLKEMQEARDTLQDITGYKTDLVRTPYGSKPSLTASQIRNLEKDGFV 204 Query: 296 LWDWNVDSDDWKYKEDSVTIYNTVMGQVHKLQKSKTNP--VILMHDQKATLKVLPRLLES 353 WDW +DS DWKY+ V+ Q+ ++ S ++ VILMHD AT+ LP L+ Sbjct: 205 YWDWTIDSMDWKYRNSQYV--TAVLQQLENMEHSSSSRPNVILMHDLPATVNALPVLINK 262 Query: 354 LKKEGYTFGIITKDMEPVN 372 LK++GY+FG++ M PV+ Sbjct: 263 LKEKGYSFGVLEDTMVPVH 281 >Q2BA98.1 Q2BA98_9BACI Polysaccharide deacetylase, putative Length = 372 Score = 184 bits (467), Expect = 1e-44, Method: Compositional matrix adjust. Identities = 116/364 (31%), Positives = 178/364 (48%), Gaps = 23/364 (6%) Query: 24 ADAAGANKSL-KLGVNDSLTKITAVSE---KNTYYVPLRALAAELKWTLT-GLPDGIQVD 78 A AA N+ + +GVNDS+ + ++T Y+P++ LAA + +++ PD + Sbjct: 22 ASAAQRNEYICAVGVNDSVLNLKQNPPFVLEHTAYMPIKDLAAAIGASISIENPDSYTIK 81 Query: 79 XXXXXXXXXXXXXXXXXQDGTVAQADTFQRDGSLMVPLKISA-YLGYRISFEPDKYLLRV 137 G A+ R+ L +P++ + + GYR+ + ++R+ Sbjct: 82 KGKSWIRYSVKQQQASTSMGKTAEWKAVVRENILFIPIRSAGEFFGYRVEYLSAGPVVRL 141 Query: 138 QDGSAALDDTAFVSKYKNELXXXXXXXXXXXXXXXXXGKTLYLTFDDGPSATTPELLDIL 197 + A + + F+S + K +YLTFDDGP+ +LDIL Sbjct: 142 VNKGAKMANEQFMSANRAAFYVKK--------------KAVYLTFDDGPAKGMESILDIL 187 Query: 198 NKYGVKATFFMLGPNMNRYPSQVKRIAEEGNGLGLHGMTHRKEKFYASASAALA-EMNGD 256 KATFFM+ P P+ VKR+ EEG+ LH +TH K K YA A EM+ Sbjct: 188 KNKKAKATFFMIEPQARANPASVKRLVEEGHYPALHSVTHDKNKLYAGNPQNPALEMDKT 247 Query: 257 NDVLRKITGTGTTLIRPPYGSKPYFTKAFRDKVLGQGYHLWDWNVDSDDWKYKEDSV-TI 315 + +TG + L+R PYGSKPY T FRD ++ +GY +WDWNVD+ DWKY S TI Sbjct: 248 RKTILSLTGIDSRLVRMPYGSKPYMTGPFRDALVQKGYKMWDWNVDTLDWKYARSSPKTI 307 Query: 316 YNTVMGQVHKLQKSKTNPVILMHDQKATLKVLPRLLESLKKEGYTFGIITKDME-PVNFW 374 + V + PVILMH T+ +LP++++ L +GY + +NFW Sbjct: 308 FENVKKGLQPAAAGGKPPVILMHVNSGTVSMLPQIIDYLYSQGYECQAYNPNAHFSMNFW 367 Query: 375 KDKR 378 KD+R Sbjct: 368 KDER 371 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.134 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,022,925,791 Number of extensions: 78108472 Number of successful extensions: 189368 Number of sequences better than 1.0e-05: 2247 Number of HSP's gapped: 185424 Number of HSP's successfully gapped: 2247 Length of query: 378 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 241 Effective length of database: 1,770,017,148 Effective search space: 426574132668 Effective search space used: 426574132668 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)