BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0391.0025.p (185 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1D9B6.1 B1D9B6_9BACL Phosphotransferase KptA/Tpt1 252 8e-66 C3CV10.1 C3CV10_BACTU Phosphotransferase 139 1e-31 C2L1P8.1 C2L1P8_LACSK RNA 2'-phosphotransferase 133 8e-30 C2ENH6.1 C2ENH6_9LACO RNA 2'-phosphotransferase 133 8e-30 C0EJU1.1 C0EJU1_NEIFL Putative uncharacterized protein 130 6e-29 >B1D9B6.1 B1D9B6_9BACL Phosphotransferase KptA/Tpt1 Length = 192 Score = 252 bits (644), Expect = 8e-66, Method: Compositional matrix adjust. Identities = 117/183 (63%), Positives = 142/183 (77%) Query: 1 MLNHAAEVSLSKFMTKLLRHTPAEYGLLLDPEDGSCLLEDLERVIHTAPKWAGITEEDIR 60 MLN A E SLSK MTK+LRHTP ++G+ LDPEDGSC + L I PKWAGIT EDI Sbjct: 4 MLNQAMEQSLSKLMTKILRHTPEQFGISLDPEDGSCGIHVLLEAIQAQPKWAGITIEDIE 63 Query: 61 QVVRNSEKQRFAIEGDRIKARYGHSHARLTYEPGIPPKVLYHGTHQAALPAILKEGLLPM 120 QVVRNSEKQRFAIE RI+ARYGHSH R+ Y G PP +LYHGT++ ALP+IL EGL PM Sbjct: 64 QVVRNSEKQRFAIEDGRIRARYGHSHDRVQYTAGEPPAILYHGTNRKALPSILGEGLHPM 123 Query: 121 GRQYIHLSEGLHFASLAGSRRGKLILLTVDTVKAVHLGVTFYYAGNEVWLSEPIPPACIA 180 RQY+HLSEG HFA+LAGSRRG+L++L +DT +A GV F++AGNEVWL++ +P C + Sbjct: 124 NRQYVHLSEGTHFATLAGSRRGELVILKIDTSRAKEAGVVFFFAGNEVWLADRVPADCCS 183 Query: 181 MRE 183 + E Sbjct: 184 VFE 186 >C3CV10.1 C3CV10_BACTU Phosphotransferase Length = 192 Score = 139 bits (351), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 79/176 (44%), Positives = 109/176 (61%), Gaps = 6/176 (3%) Query: 10 LSKFMTKLLRHTPAEYGLLLDPEDGSCLLEDLERVIHTAPKWAG-ITEEDIRQVVRNSEK 68 L F+ K+LRH+P+++ + LD E G +++L I + KW +T E I +VV+ EK Sbjct: 12 LGGFLCKILRHSPSQFHVSLD-EYGYAPIDELLNAISSNEKWVNLVTVEMILEVVKTDEK 70 Query: 69 QRFAIE--GDRIKARYGHSHARLTYEPG--IPPKVLYHGTHQAALPAILKEGLLPMGRQY 124 QR+ ++ G +IKARYGHS A + E + P +LYHGT L +I K GLLPM R Sbjct: 71 QRYKLDSTGTKIKARYGHSIAVKSDEEQNRVLPDILYHGTGMEHLDSIQKLGLLPMKRVN 130 Query: 125 IHLSEGLHFASLAGSRRGKLILLTVDTVKAVHLGVTFYYAGNEVWLSEPIPPACIA 180 +HLSE FA+ + R +LL VDT+ A +LGVT YAG+EVWLS PIP C+ Sbjct: 131 VHLSETTFFANKSAKRNQFPVLLKVDTIMAKNLGVTVEYAGDEVWLSSPIPSDCLT 186 >C2L1P8.1 C2L1P8_LACSK RNA 2'-phosphotransferase Length = 188 Score = 133 bits (334), Expect = 8e-30, Method: Compositional matrix adjust. Identities = 77/178 (43%), Positives = 105/178 (58%), Gaps = 8/178 (4%) Query: 3 NHAAEVSLSKFMTKLLRHTPAEYGLLLDPE---DGSCLLEDLERVIHTAPKWAGITEEDI 59 +H ++V +SK M+ LRH P +YGL LD D L + R+ H PK +TE I Sbjct: 6 DHRSDVFISKKMSYCLRHNPGKYGLKLDEYGFVDLQEFLNAMNRIHHFQPK---LTEMKI 62 Query: 60 RQVVRNSEKQRFAIEGDRIKARYGHS-HARLTYEPGIPPKVLYHGTHQAALPAILKEGLL 118 R+++ NS+K+RF I+ +I A YGHS + + IPPKVLYHGT L +I +EGLL Sbjct: 63 REIMHNSDKERFEIKNGKICALYGHSLPGIIKRKKAIPPKVLYHGTAHRFLTSIEREGLL 122 Query: 119 PMGRQYIHLSEGLHFASLAGSRRGKL-ILLTVDTVKAVHLGVTFYYAGNEVWLSEPIP 175 PMGRQY+HLS + A G RR +L V+ KA G+ FY +EVWL + +P Sbjct: 123 PMGRQYVHLSTDIAMAESVGKRRDHHPAILLVNAEKAHKDGIDFYIGNDEVWLCDCVP 180 >C2ENH6.1 C2ENH6_9LACO RNA 2'-phosphotransferase Length = 188 Score = 133 bits (334), Expect = 8e-30, Method: Compositional matrix adjust. Identities = 77/178 (43%), Positives = 105/178 (58%), Gaps = 8/178 (4%) Query: 3 NHAAEVSLSKFMTKLLRHTPAEYGLLLDPE---DGSCLLEDLERVIHTAPKWAGITEEDI 59 +H ++V +SK M+ LRH P +YGL LD D L + R+ H PK +TE I Sbjct: 6 DHRSDVFISKKMSYCLRHNPGKYGLKLDEYGFVDLQEFLNAMNRIHHFQPK---LTEMKI 62 Query: 60 RQVVRNSEKQRFAIEGDRIKARYGHS-HARLTYEPGIPPKVLYHGTHQAALPAILKEGLL 118 R+++ NS+K+RF I+ +I A YGHS + + IPPKVLYHGT L +I +EGLL Sbjct: 63 REIMHNSDKERFEIKNGKICALYGHSLPGIIKRKKAIPPKVLYHGTAHRFLTSIEREGLL 122 Query: 119 PMGRQYIHLSEGLHFASLAGSRRGKL-ILLTVDTVKAVHLGVTFYYAGNEVWLSEPIP 175 PMGRQY+HLS + A G RR +L V+ KA G+ FY +EVWL + +P Sbjct: 123 PMGRQYVHLSTDIAMAESVGKRRDHHPAILLVNAEKAHKDGIDFYIGNDEVWLCDCVP 180 >C0EJU1.1 C0EJU1_NEIFL Putative uncharacterized protein Length = 189 Score = 130 bits (326), Expect = 6e-29, Method: Compositional matrix adjust. Identities = 73/186 (39%), Positives = 112/186 (60%), Gaps = 6/186 (3%) Query: 1 MLNHAAEVSLSKFMTKLLRHTPAEYGLLLDPEDGSCLLEDLERVIHTAPKWAG--ITEED 58 MLN S+SKF++ +LRH P + G+ LD + + L +V H + G +T E Sbjct: 2 MLNEKQLKSISKFLSFILRHHPEQIGITLDKDGWVDIDTLLTQVNHPERGFTGNPLTYEV 61 Query: 59 IRQVVRNSEKQRFAI--EGDRIKARYGHSHARLTYEPGI--PPKVLYHGTHQAALPAILK 114 + +VV N++K+RF + +G RI+A GHS A++ E + PP++LYHGT + ++P+I++ Sbjct: 62 LLEVVENNDKKRFTLSEDGKRIRAAQGHSTAQVQVEHKVATPPQILYHGTAERSVPSIME 121 Query: 115 EGLLPMGRQYIHLSEGLHFASLAGSRRGKLILLTVDTVKAVHLGVTFYYAGNEVWLSEPI 174 +GL R ++HLS A GSR GK ++LT+DTV + G FY A N VWL+E + Sbjct: 122 QGLHSASRHFVHLSADAATAVNVGSRHGKPVVLTIDTVAMLAAGHQFYLADNGVWLTENV 181 Query: 175 PPACIA 180 PP I Sbjct: 182 PPQFIG 187 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.138 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,140,667,099 Number of extensions: 44866712 Number of successful extensions: 121900 Number of sequences better than 1.0e-05: 266 Number of HSP's gapped: 121568 Number of HSP's successfully gapped: 266 Length of query: 185 Length of database: 3,060,696,203 Length adjustment: 127 Effective length of query: 58 Effective length of database: 1,864,227,298 Effective search space: 108125183284 Effective search space used: 108125183284 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 127 (53.5 bits)