BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0391.0015.p (215 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1D5N5.1 B1D5N5_9BACL Nitroreductase 219 2e-55 A6CKD1.1 A6CKD1_9BACI NAD(P)H nitroreductase 217 7e-55 C0ZEM8.1 C0ZEM8_BREBN Putative NAD(P)H nitroreductase 216 1e-54 B3KCU8.1 B3KCU8_9BACI Nitroreductase 199 2e-49 C3BLL5.1 C3BLL5_9BACI Nitroreductase 194 7e-48 >B1D5N5.1 B1D5N5_9BACL Nitroreductase Length = 214 Score = 219 bits (557), Expect = 2e-55, Method: Compositional matrix adjust. Identities = 103/211 (48%), Positives = 141/211 (66%) Query: 1 MASELKVEQEFSKVIRERRSVRKYDSSWKISDXXXXXXXXXXXXXPSSSNLQPWRFIVIT 60 MA E +V ++F++V+ RRSVR YD S+KISD PSS+N Q WRF+VIT Sbjct: 1 MAIETQVGKDFTEVLTSRRSVRHYDPSFKISDEEIKEILELATKAPSSANTQSWRFLVIT 60 Query: 61 DQEIKAKLLPIAHNQAQTTESSAMIAVLGDTEAYSNAEKIYGHASATGLMPEDVAAAMAK 120 DQ +K +LLPIA+NQ Q ++SA++ +LGD E Y+ +I G A G M E++A Sbjct: 61 DQALKDQLLPIANNQQQVVDASAVVVILGDLEFYTKLGEINGAAVKAGYMTEEIAQRFTD 120 Query: 121 RSREGYSSLPKEKLEDIALVDGGLVSMQLMLAAKARGYDTVPMGGYDAAALKELFRISDR 180 + S+PKE++ +I D GLVSMQ+MLAAK++GYDTVPMGGYD LK F I +R Sbjct: 121 HYTSLFPSIPKERVTEIVAFDAGLVSMQIMLAAKSKGYDTVPMGGYDRDKLKAFFEIPER 180 Query: 181 YIPVMLIALGKAAVEGRPTNRLPLEAVVHWN 211 Y+P++++ +GKA EGRPT RLPL+ + WN Sbjct: 181 YLPLLVLPIGKAKEEGRPTVRLPLDEITFWN 211 >A6CKD1.1 A6CKD1_9BACI NAD(P)H nitroreductase Length = 235 Score = 217 bits (552), Expect = 7e-55, Method: Compositional matrix adjust. Identities = 104/206 (50%), Positives = 132/206 (64%) Query: 8 EQEFSKVIRERRSVRKYDSSWKISDXXXXXXXXXXXXXPSSSNLQPWRFIVITDQEIKAK 67 + +F V +ERRSVR YD +K+ + PSSSNLQPWRF+VI DQ+ K + Sbjct: 30 DTDFLTVAKERRSVRHYDPDFKLEESEIRELLETATLAPSSSNLQPWRFLVIEDQKEKER 89 Query: 68 LLPIAHNQAQTTESSAMIAVLGDTEAYSNAEKIYGHASATGLMPEDVAAAMAKRSREGYS 127 LLPIA+NQ Q ++S + AVLGD EAY NA++IY + G MPEDV E Y Sbjct: 90 LLPIANNQQQIVDASVVFAVLGDREAYKNADRIYSQIAEKGNMPEDVKDMYVNSIFENYG 149 Query: 128 SLPKEKLEDIALVDGGLVSMQLMLAAKARGYDTVPMGGYDAAALKELFRISDRYIPVMLI 187 + PKE+ IA +DGG+V+ QLMLAAKA+GYDTVPMGGYD A E F + + Y P+MLI Sbjct: 150 NFPKERQAKIAHIDGGIVANQLMLAAKAKGYDTVPMGGYDEAKFMEAFNVPETYEPIMLI 209 Query: 188 ALGKAAVEGRPTNRLPLEAVVHWNGF 213 +LGK G RLPL+ V+ WN F Sbjct: 210 SLGKGTKAGFEKTRLPLDDVMQWNKF 235 >C0ZEM8.1 C0ZEM8_BREBN Putative NAD(P)H nitroreductase Length = 215 Score = 216 bits (550), Expect = 1e-54, Method: Compositional matrix adjust. Identities = 109/204 (53%), Positives = 134/204 (65%) Query: 8 EQEFSKVIRERRSVRKYDSSWKISDXXXXXXXXXXXXXPSSSNLQPWRFIVITDQEIKAK 67 ++ F VIRERRSVR YD + KIS PSSSNLQPWRF++I +Q +K K Sbjct: 7 QKAFKDVIRERRSVRHYDPTAKISRDELKEMLKEATLAPSSSNLQPWRFLIIDEQPLKEK 66 Query: 68 LLPIAHNQAQTTESSAMIAVLGDTEAYSNAEKIYGHASATGLMPEDVAAAMAKRSREGYS 127 LLPIA NQ Q E++A+IAVL D E Y A IY A G M E+V A + + Y Sbjct: 67 LLPIAFNQKQVVEAAAIIAVLADYEGYKQAGSIYKKAVEAGYMTEEVKATLIDNINKRYE 126 Query: 128 SLPKEKLEDIALVDGGLVSMQLMLAAKARGYDTVPMGGYDAAALKELFRISDRYIPVMLI 187 + + +++IALVDGGLVSMQLML AKARGYDTVPMGGY++ KE F+I D+Y VMLI Sbjct: 127 NRDRAIIKEIALVDGGLVSMQLMLVAKARGYDTVPMGGYNSEKFKEAFQIPDQYETVMLI 186 Query: 188 ALGKAAVEGRPTNRLPLEAVVHWN 211 A+GKAA G PT RL +E + WN Sbjct: 187 AVGKAAEPGHPTTRLDVEEITFWN 210 >B3KCU8.1 B3KCU8_9BACI Nitroreductase Length = 213 Score = 199 bits (505), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 104/211 (49%), Positives = 128/211 (60%) Query: 1 MASELKVEQEFSKVIRERRSVRKYDSSWKISDXXXXXXXXXXXXXPSSSNLQPWRFIVIT 60 M++ + +F VI+ER SVR+YD S KI PSSSNLQPWRF+VI Sbjct: 1 MSTTTTTKPDFQTVIQERHSVRQYDPSVKIPREEMEAILKDATLAPSSSNLQPWRFLVID 60 Query: 61 DQEIKAKLLPIAHNQAQTTESSAMIAVLGDTEAYSNAEKIYGHASATGLMPEDVAAAMAK 120 D ++ KL PIA+NQ ++SA+IAVLGD + Y EKIY + G M E+V Sbjct: 61 DADLLKKLHPIANNQEHVLQASAVIAVLGDLKWYEKGEKIYSMSEEAGYMTEEVKQRFIH 120 Query: 121 RSREGYSSLPKEKLEDIALVDGGLVSMQLMLAAKARGYDTVPMGGYDAAALKELFRISDR 180 + Y++L E I VD GL+SMQLML+A+ARGYDTVPMGGY+ E F I Sbjct: 121 TAENMYANLDPSIKESIVDVDAGLISMQLMLSARARGYDTVPMGGYNKVRFVEEFHIPSH 180 Query: 181 YIPVMLIALGKAAVEGRPTNRLPLEAVVHWN 211 Y VMLIA+GKAA GRPT RLPLE V WN Sbjct: 181 YRSVMLIAIGKAAAPGRPTVRLPLEDVAFWN 211 >C3BLL5.1 C3BLL5_9BACI Nitroreductase Length = 212 Score = 194 bits (492), Expect = 7e-48, Method: Compositional matrix adjust. Identities = 96/204 (47%), Positives = 131/204 (64%), Gaps = 2/204 (0%) Query: 9 QEFSKVIRERRSVRKYDSSWKISDXXXXXXXXXXXXXPSSSNLQPWRFIVITDQEIKAKL 68 +F++++ RRS+RKYD + KIS PSS N+QPWRF+VI +E KA L Sbjct: 8 NDFTEILTGRRSIRKYDPAVKISKEEMTEILTEATLAPSSVNMQPWRFLVIDSEEGKATL 67 Query: 69 LPIA-HNQAQTTESSAMIAVLGDTEAYSNAEKIYGHASATGLMPEDVAAAMAKRSREGYS 127 P+A NQ Q SSAMIAV GD + + NAE+IYG A GLMP +V A ++ +S Sbjct: 68 APLAKFNQLQVETSSAMIAVFGDLQNFDNAEEIYGTAVERGLMPAEVKKAQMQKLSAYFS 127 Query: 128 SLPKEKLEDIALVDGGLVSMQLMLAAKARGYDTVPMGGYDAAALKELFRIS-DRYIPVML 186 S+ E ++D L+DGGL SMQ MLAA+A GYDT P+GG++ + E F + DRY+PVML Sbjct: 128 SVSPEVMKDTVLIDGGLASMQFMLAARAHGYDTCPIGGFEKDKIAEAFGLDKDRYVPVML 187 Query: 187 IALGKAAVEGRPTNRLPLEAVVHW 210 I++GKAA G + RLP++ + W Sbjct: 188 ISIGKAADSGYKSVRLPIDKIAQW 211 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.129 0.363 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,080,063,792 Number of extensions: 38662332 Number of successful extensions: 114352 Number of sequences better than 1.0e-05: 958 Number of HSP's gapped: 114019 Number of HSP's successfully gapped: 958 Length of query: 215 Length of database: 3,060,696,203 Length adjustment: 130 Effective length of query: 85 Effective length of database: 1,835,964,253 Effective search space: 156056961505 Effective search space used: 156056961505 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 128 (53.9 bits)