BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0391.0008.p (443 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KCE4.1 B3KCE4_9BACI Chloride channel core 538 e-151 B1DJR0.1 B1DJR0_9BACL Chloride channel core 516 e-144 B2TNJ2.1 B2TNJ2_CLOBB Voltage-gated chloride channel 473 e-131 B2V4L1.1 B2V4L1_CLOBA Voltage-gated chloride channel 469 e-130 B1BH44.1 B1BH44_CLOPE Voltage-gated chloride channel family protein 445 e-123 >B3KCE4.1 B3KCE4_9BACI Chloride channel core Length = 441 Score = 538 bits (1385), Expect = e-151, Method: Compositional matrix adjust. Identities = 275/436 (63%), Positives = 333/436 (76%), Gaps = 3/436 (0%) Query: 8 WLKLMERSHFLALWGTFIKWIVLGGCVGILSGSASAIFLKSLDAVTEVRLNHAWXXXXXX 67 W++ + L G FIKW+VLG VG+++GSASA FL SLD VT +RL + W Sbjct: 5 WIESAGKWSLAMLSGYFIKWVVLGSLVGLMTGSASAFFLYSLDYVTSLRLGNPWLLYLLP 64 Query: 68 XXXXXVSFLYMRYGKNSAKGNNLILEQIQQGNETIPMRMAPLVLFGTLVTHLFGGSAGRE 127 VSFLY ++GK+SAKGNNLILEQI G E+IP+RMAPLVL GTL+THLFGGSAGRE Sbjct: 65 LGGAAVSFLYYKFGKSSAKGNNLILEQIHGGTESIPLRMAPLVLIGTLITHLFGGSAGRE 124 Query: 128 GTAVQMGGSLAEGFGRLIRINSLDRQILLMCXXXXXXXXXXXTPLAGTLFGLEVVAIGLI 187 GTAVQMGGSL+E G+LIR+ DR+ILL+C TPLAGTLFGLEV+AIGL+ Sbjct: 125 GTAVQMGGSLSEWLGKLIRVTPSDRKILLICGISGGFGSIFGTPLAGTLFGLEVLAIGLV 184 Query: 188 SHKALLPCFTASFIGDIVATRLWKVQHIHYHVELFPPLTAIILLKVILASVLFGLASILF 247 SH+ALLP F AS +G++ AT LW V H+HY + P L ++++KV+ AS+LFGL S LF Sbjct: 185 SHQALLPAFAASMVGNLTATSLWGVSHLHYPIGDIPALGFMVIMKVVFASMLFGLTSRLF 244 Query: 248 SELTHYLKKTFTRLVKNPMLKSALGGFIIIALVYIAGTRDYLGLGIPLIQDSFQTDVAPF 307 SELTH LK+ + + NPM+KSA GG III LVY+ GTRDYLGLGIPLI+ +F +V+PF Sbjct: 245 SELTHSLKRWYGFVFHNPMVKSAAGGVIIIGLVYLLGTRDYLGLGIPLIEAAFAGEVSPF 304 Query: 308 AFLWKLIFTSFTLGTGFQGGEVTPLFAIGATLGNSLASFLHLHAPFLASLGFIAVFCGAT 367 AFL K+IFTS TLG GFQGGEVTPLFAIGATLG++L+ ++ LHAPFLA+LGFIAVFCGAT Sbjct: 305 AFLGKIIFTSLTLGAGFQGGEVTPLFAIGATLGSALSEWIGLHAPFLAALGFIAVFCGAT 364 Query: 368 NTPVACFLMGIELFGSGGAVYMFIACLISYLFSGHSGIYTSQQIGISKSEWVRIPEGTTL 427 NTP+ACF+MGIELFG GAVYMF+AC+ISYLFSGHSGIYTSQ IG SK + P GTTL Sbjct: 365 NTPIACFIMGIELFGGEGAVYMFMACVISYLFSGHSGIYTSQLIGTSKHPQLSYPPGTTL 424 Query: 428 GTAK---QLRTTKKTS 440 +K + + KKTS Sbjct: 425 AGSKAFNKRKDNKKTS 440 >B1DJR0.1 B1DJR0_9BACL Chloride channel core Length = 440 Score = 516 bits (1330), Expect = e-144, Method: Compositional matrix adjust. Identities = 271/428 (63%), Positives = 333/428 (77%), Gaps = 1/428 (0%) Query: 13 ERSHFLALWGTFIKWIVLGGCVGILSGSASAIFLKSLDAVTEVRLNHAWXXXXXXXXXXX 72 E+ +AL + +KWI GG VGILSGSASA+FL SLD T RLNH W Sbjct: 12 EKVMHVALLASLLKWIFYGGLVGILSGSASALFLASLDYATGQRLNHPWLLFLLPFGGAL 71 Query: 73 VSFLYMRYGKNSAKGNNLILEQIQQGNETIPMRMAPLVLFGTLVTHLFGGSAGREGTAVQ 132 +SF Y ++GKN+ KGNNLILEQI+QG ETIP RMAPLVL GTL+THLFGGSAGREGTAVQ Sbjct: 72 MSFFYWKHGKNAGKGNNLILEQIRQGQETIPFRMAPLVLLGTLLTHLFGGSAGREGTAVQ 131 Query: 133 MGGSLAEGFGRLIRINSLDRQILLMCXXXXXXXXXXXTPLAGTLFGLEVVAIGLISHKAL 192 MGGS +E G++ +++ DR+ILLMC TPLAGT+FG+EV+AIG + ++A+ Sbjct: 132 MGGSFSELIGKVFKVDETDRKILLMCGISSGFGSVFGTPLAGTVFGIEVIAIGFVRYQAI 191 Query: 193 LPCFTASFIGDIVATRLWKVQHIHYHVELFPPLTAIILLKVILASVLFGLASILFSELTH 252 LPCF ASF+G +V T W + HIHY + P LTAI+++KV+ AS++FGLASI+FSELTH Sbjct: 192 LPCFIASFVGHLVTTA-WGIHHIHYAMGKVPDLTAIVVIKVVFASIIFGLASIMFSELTH 250 Query: 253 YLKKTFTRLVKNPMLKSALGGFIIIALVYIAGTRDYLGLGIPLIQDSFQTDVAPFAFLWK 312 +LKKTF++L KNP++K+ +GG ++I LVY+ GTR+YLGLGIPL+QDSF+ VAP AFLWK Sbjct: 251 WLKKTFSKLFKNPVIKTFIGGVVVIVLVYLLGTREYLGLGIPLLQDSFEYPVAPLAFLWK 310 Query: 313 LIFTSFTLGTGFQGGEVTPLFAIGATLGNSLASFLHLHAPFLASLGFIAVFCGATNTPVA 372 IFT+ TLG GFQGGEVTPLF IGATLGN+LA LHL APFLA LGFIAVF GATNTP+A Sbjct: 311 TIFTALTLGAGFQGGEVTPLFVIGATLGNALAGLLHLAAPFLAGLGFIAVFSGATNTPIA 370 Query: 373 CFLMGIELFGSGGAVYMFIACLISYLFSGHSGIYTSQQIGISKSEWVRIPEGTTLGTAKQ 432 CF+MGIELFGS GA+YMF+AC+ISYLFSGH+GIYTSQQIGISKS IPE TL + ++ Sbjct: 371 CFIMGIELFGSEGALYMFMACMISYLFSGHTGIYTSQQIGISKSRLHLIPENATLASIRE 430 Query: 433 LRTTKKTS 440 + K + Sbjct: 431 NKKQNKPA 438 >B2TNJ2.1 B2TNJ2_CLOBB Voltage-gated chloride channel Length = 457 Score = 473 bits (1218), Expect = e-131, Method: Compositional matrix adjust. Identities = 230/421 (54%), Positives = 304/421 (72%), Gaps = 1/421 (0%) Query: 23 TFIKWIVLGGCVGILSGSASAIFLKSLDAVTEVRLNHAWXXXXXXXXXXXVSFLYMRYGK 82 +F+KWI +G +G L+G A A+FL SL+ T +R N++W VSFLY +YGK Sbjct: 27 SFLKWIFIGTIIGCLTGVAGALFLNSLELATTLRNNNSWLLFLLPIGGAFVSFLYSKYGK 86 Query: 83 NSAKGNNLILEQIQQGNETIPMRMAPLVLFGTLVTHLFGGSAGREGTAVQMGGSLAEGFG 142 +S+KGNNLI+++I N IPMRMA LV GT VTHL GGSAGREGT VQ+G S++EG G Sbjct: 87 SSSKGNNLIIDKINTNNGNIPMRMASLVFLGTFVTHLLGGSAGREGTGVQIGSSISEGVG 146 Query: 143 RLIRINSLDRQILLMCXXXXXXXXXXXTPLAGTLFGLEVVAIGLISHKALLPCFTASFIG 202 RL++++ +D +I+LMC TPLAGT+FGLEV +G +S++AL+PCFT++FIG Sbjct: 147 RLLKLDKVDTKIILMCGISSGFSSVFGTPLAGTIFGLEVATLGTMSYQALIPCFTSAFIG 206 Query: 203 DIVATRLWKVQHIHYHVELFPPLTAIILLKVILASVLFGLASILFSELTHYLKKTFTRLV 262 +IV T + V H HY + P +T ++LK+I+A++LFGL S LFSELTH LK F+ Sbjct: 207 NIVTTA-FGVHHSHYSILEVPTITYFVVLKIIIAAILFGLVSKLFSELTHKLKDIFSSRF 265 Query: 263 KNPMLKSALGGFIIIALVYIAGTRDYLGLGIPLIQDSFQTDVAPFAFLWKLIFTSFTLGT 322 +N +KS +GGFIII L Y+ GTRDYLGL +PLI +SF V+PFAF KLIFTSFTLGT Sbjct: 266 ENAAIKSMVGGFIIIILTYLIGTRDYLGLSLPLINNSFTEQVSPFAFFNKLIFTSFTLGT 325 Query: 323 GFQGGEVTPLFAIGATLGNSLASFLHLHAPFLASLGFIAVFCGATNTPVACFLMGIELFG 382 GFQGGEVTPLF IG+T GN+L++ LH+ FLA+LG I VF GATN P+ F++G+ELFG Sbjct: 326 GFQGGEVTPLFVIGSTFGNTLSNILHISPSFLAALGLIGVFAGATNAPITSFILGLELFG 385 Query: 383 SGGAVYMFIACLISYLFSGHSGIYTSQQIGISKSEWVRIPEGTTLGTAKQLRTTKKTSAE 442 + G +MF+ C ISYLFSGHSGIY SQ+IGISKS +++PE +TL K+ + + +S Sbjct: 386 AQGIEFMFMTCAISYLFSGHSGIYISQKIGISKSTLIKVPEESTLAYYKKSKNEQSSSQN 445 Query: 443 E 443 Sbjct: 446 N 446 >B2V4L1.1 B2V4L1_CLOBA Voltage-gated chloride channel Length = 448 Score = 469 bits (1206), Expect = e-130, Method: Compositional matrix adjust. Identities = 229/424 (54%), Positives = 304/424 (71%), Gaps = 4/424 (0%) Query: 23 TFIKWIVLGGCVGILSGSASAIFLKSLDAVTEVRLNHAWXXXXXXXXXXXVSFLYMRYGK 82 +F+KWI LG VG L+G A+FL SL+ T +R N+ W VSFLY +YGK Sbjct: 20 SFLKWIFLGTVVGCLTGLVGALFLNSLEIATSLRNNNPWLLFLLPLGGALVSFLYSKYGK 79 Query: 83 NSAKGNNLILEQIQQGNETIPMRMAPLVLFGTLVTHLFGGSAGREGTAVQMGGSLAEGFG 142 +S++GNNLI+E+I N IP+RMA LV GT +THLFGGSAGREGT VQ+G S++EG Sbjct: 80 SSSRGNNLIIEKINTSNGEIPLRMASLVFIGTFITHLFGGSAGREGTGVQIGSSISEGIS 139 Query: 143 RLIRINSLDRQILLMCXXXXXXXXXXXTPLAGTLFGLEVVAIGLISHKALLPCFTASFIG 202 L +++ +D +I+LMC TPLAGT+FGLEV A+G +S++AL+PCFT++F+G Sbjct: 140 HLFKLDKVDTKIILMCGISSGFSSVFGTPLAGTMFGLEVAALGTMSYQALIPCFTSAFVG 199 Query: 203 DIVATRLWKVQHIHYHVELFPPLTAIILLKVILASVLFGLASILFSELTHYLKKTFTRLV 262 +IV T + V H HY + P +T I++ KVI+A++LFGL S FSE TH LK F+ Sbjct: 200 NIVTTA-FGVSHSHYSILEVPSITYIVVFKVIVAAILFGLVSKFFSEFTHKLKDIFSSTF 258 Query: 263 KNPMLKSALGGFIIIALVYIAGTRDYLGLGIPLIQDSFQTDVAPFAFLWKLIFTSFTLGT 322 KN +KS +GGFI+I L Y+ GTRDYLGL +PLI +SF +V+PFAF KLIFTSFTLGT Sbjct: 259 KNSAIKSMVGGFIVIILTYLIGTRDYLGLSLPLINNSFTENVSPFAFFNKLIFTSFTLGT 318 Query: 323 GFQGGEVTPLFAIGATLGNSLASFLHLHAPFLASLGFIAVFCGATNTPVACFLMGIELFG 382 GFQGGEVTPLF IG+TLGN+L++ LH+ FLA+LG I VF GATN+P+ F++G+ELFG Sbjct: 319 GFQGGEVTPLFVIGSTLGNTLSTILHISPSFLAALGLIGVFAGATNSPITSFILGLELFG 378 Query: 383 SGGAVYMFIACLISYLFSGHSGIYTSQQIGISKSEWVRIPEGTTLGTAK---QLRTTKKT 439 + G +MF+ C ISYLFSGHSGIY SQ+IGISKS +++P+ +TL + K L T T Sbjct: 379 AQGIEFMFMTCAISYLFSGHSGIYISQKIGISKSRLIKVPKESTLASYKNNINLNTESNT 438 Query: 440 SAEE 443 + ++ Sbjct: 439 NKDD 442 >B1BH44.1 B1BH44_CLOPE Voltage-gated chloride channel family protein Length = 437 Score = 445 bits (1144), Expect = e-123, Method: Compositional matrix adjust. Identities = 226/437 (51%), Positives = 299/437 (68%), Gaps = 1/437 (0%) Query: 1 MRAIHTYWLKLMERSHFLALWGTFIKWIVLGGCVGILSGSASAIFLKSLDAVTEVRLNHA 60 M+ +LK + ++ L TF+KW+++G +G G AIFLKSL+ T+ R+NH Sbjct: 1 MKQKSKKYLKYISSNNTFLLGKTFLKWLLIGSLIGFFVGGVIAIFLKSLEWATDYRVNHG 60 Query: 61 WXXXXXXXXXXXVSFLYMRYGKNSAKGNNLILEQIQQGNETIPMRMAPLVLFGTLVTHLF 120 W VSFLY ++G+NS KGNNLI+E I +P+RM PLV FGT+VTH F Sbjct: 61 WILFLLPIGGALVSFLYYKFGRNSVKGNNLIIENINDYCGDVPLRMVPLVFFGTVVTHFF 120 Query: 121 GGSAGREGTAVQMGGSLAEGFGRLIRINSLDRQILLMCXXXXXXXXXXXTPLAGTLFGLE 180 GGSAGREGT VQ+G S+AE G+L+++N D +I+LM TPLAGT+FGLE Sbjct: 121 GGSAGREGTGVQIGASIAETIGKLLKLNKEDSKIMLMAGVSGGFSAIFGTPLAGTIFGLE 180 Query: 181 VVAIGLISHKALLPCFTASFIGDIVATRLWKVQHIHYHVELFPPLTAIILLKVILASVLF 240 V +G +S++AL+P F AS +G+ V L V H HY V P ++A I+LKVI++++ F Sbjct: 181 VSVLGKMSYEALIPSFFASIVGNEVVKNL-GVHHSHYKVLGVPDISAFIILKVIISAICF 239 Query: 241 GLASILFSELTHYLKKTFTRLVKNPMLKSALGGFIIIALVYIAGTRDYLGLGIPLIQDSF 300 GLAS LFSELTH LK F+ KN LKS +GG I+I LVY+ G+R YLGL +PL+ ++F Sbjct: 240 GLASRLFSELTHKLKGLFSNKFKNTSLKSFVGGCIVILLVYLIGSRKYLGLSLPLLSEAF 299 Query: 301 QTDVAPFAFLWKLIFTSFTLGTGFQGGEVTPLFAIGATLGNSLASFLHLHAPFLASLGFI 360 V+PF FL KLIFTS TLG G+QGGEVTPLF IG+TLGN+L+ L+L FLASLG + Sbjct: 300 NGHVSPFDFLGKLIFTSITLGAGYQGGEVTPLFVIGSTLGNTLSGILNLSPSFLASLGLV 359 Query: 361 AVFCGATNTPVACFLMGIELFGSGGAVYMFIACLISYLFSGHSGIYTSQQIGISKSEWVR 420 VF GATNTP+A F++GIE+FG GGA Y+F+AC ISY+FSGH+GIYTSQ+IG+SKS + Sbjct: 360 GVFAGATNTPIASFVLGIEMFGVGGAPYLFMACAISYIFSGHTGIYTSQKIGVSKSNLIN 419 Query: 421 IPEGTTLGTAKQLRTTK 437 +TL + + K Sbjct: 420 FSHDSTLASLSKREEVK 436 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.327 0.142 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,410,597,102 Number of extensions: 96033311 Number of successful extensions: 298830 Number of sequences better than 1.0e-05: 1344 Number of HSP's gapped: 296928 Number of HSP's successfully gapped: 1345 Length of query: 443 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 304 Effective length of database: 1,751,175,118 Effective search space: 532357235872 Effective search space used: 532357235872 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 133 (55.8 bits)