BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0389.0014.p (405 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KEH1.1 B3KEH1_9BACI ABC transporter EcsB 373 e-101 B1D7A5.1 B1D7A5_9BACL ABC transporter EcsB 129 4e-28 A8FBL9.1 A8FBL9_BACP2 ABC superfamily ATP binding cassette trans... 96 9e-18 C2URD8.1 C2URD8_BACCE ABC transporter EcsB 90 3e-16 C3BY76.1 C3BY76_BACTU ABC transporter EcsB 89 6e-16 >B3KEH1.1 B3KEH1_9BACI ABC transporter EcsB Length = 407 Score = 373 bits (958), Expect = e-101, Method: Compositional matrix adjust. Identities = 194/398 (48%), Positives = 264/398 (66%) Query: 1 MDLKELRRQRRSRFMGGLTPYVGYIIQSGIAMVFLLVLIIFSAWYTSLLRDVPEGIPIRW 60 M+L+EL +RR +F G + PY+GY+IQSG+A+VFL +LI F+AWYT+LL+ VP +PIRW Sbjct: 1 MNLRELYVKRRGQFWGEILPYLGYVIQSGVAVVFLFLLIAFAAWYTALLQQVPPDLPIRW 60 Query: 61 IMLVLLLPAAAHSSFRTYLQSPDTIFLLPQGHRMKEYFAPAWTAGNVWKILRLAFVLITL 120 IML+ L P +SS RTYL++ DT+FL+PQ RMKEYF AW +G ++KI+ L VL+ L Sbjct: 61 IMLIFLAPLMINSSIRTYLRTADTVFLIPQESRMKEYFRKAWFSGVIYKIIGLGLVLLIL 120 Query: 121 WPLYIRTDDSHQMLFATLAVLILVKILASFGLWRELAMLSRPASMGYSLLRWAVSGLMIA 180 WPLYIR+D S + L AT AVL + K+L+S+G W+E M+SR A+ Y LRW V L +A Sbjct: 121 WPLYIRSDVSPKTLLATFAVLTVFKLLSSYGSWKEQLMVSRNAAAAYRALRWIVPALSLA 180 Query: 181 AWLWQPXXXXXXXXXXXXXXXXXXXXXXXXXSVPWERLIATERNQGARALMILGWFVDVP 240 AWLW P V WERLIA E+ Q +R +M+L WFV+VP Sbjct: 181 AWLWHPTGRALLFMALLGLTYVAALSVPVKHLVAWERLIAVEQRQASRVMMVLSWFVNVP 240 Query: 241 GREQRVYPRRYLSKWGAGIAWRRSTAYRFLLTKSFARGDIFGIVIRIFLLALLLVWWNRE 300 REQRV+ R++LS WG G+AW++ +AYRFLL KS R DI GI+IR+ LL +VW R+ Sbjct: 241 QREQRVHARKWLSGWGKGLAWKKESAYRFLLIKSLTRSDILGILIRVGLLGAFIVWATRD 300 Query: 301 SYIGSGIYLFFVLVMGVQLSALRKLHSESFWLTVYPLPEGSKASSTIQFIFRAHXXXXXX 360 S + + +YL ++++G+QLSALRKLH ESFWL VYPLPEG++ + + IF+ Sbjct: 301 SLVSALVYLISLMIVGLQLSALRKLHPESFWLHVYPLPEGTRRDNEGKLIFQVQLFWAVL 360 Query: 361 XXXXXXXGIAGRPLPVIGTFLAGALLAYLFKSRASRKS 398 GI P ++GT AG + +LF+S A+RKS Sbjct: 361 LWLPLVPGILSAPGRILGTMAAGIAIVFLFRSAAARKS 398 >B1D7A5.1 B1D7A5_9BACL ABC transporter EcsB Length = 417 Score = 129 bits (325), Expect = 4e-28, Method: Compositional matrix adjust. Identities = 103/351 (29%), Positives = 150/351 (42%), Gaps = 6/351 (1%) Query: 2 DLKELRRQRRSRFMGGLTPYVGYIIQSGIAMVFLLVLIIFSAWYTSLLRDVPEGIPIRWI 61 L+ L +R F +TPY GY+ QSG + +++ + Y L+R +P G PI + Sbjct: 7 SLEALWLKRSGEFRKEVTPYFGYMAQSGFPLFMSFIVLSSAFGYIKLIRGLPSGFPITLV 66 Query: 62 MLVLLLPAAAHSSFRTYLQSPDTIFLLPQGHRMKEYFAPAWTAGNVWKILRLAFVLITLW 121 V L + S RT+L SPD +F +P+ M Y ++ + L VL+ W Sbjct: 67 GTVALFIILSWSPLRTWLTSPDIVFHMPREAEMGSYIRRSFRRSAILTSLLGFIVLLLYW 126 Query: 122 PLYIRTDDSHQMLFATLAVLILVKILASFGLWRELAMLSRPASMGYSLLRWAVSGLMIAA 181 P+Y R D L + +++ +G W+E + + LLRWA + L + Sbjct: 127 PIY-RQGDGEAGFLTLLLLCAVMRAANLWGAWKERKIAWSGVRLLLRLLRWAANALTLVI 185 Query: 182 WLWQPXXXXXXXXXXXXXXXXXXXXXXXXXSVPWERLIATERNQGARALMILGWFVDVPG 241 L S PWERLI E G F+DVP Sbjct: 186 MLTSAPWQAALFLLLLLVLFALLYRLPVQHSFPWERLIEEEVRTRRSYYQFFGLFIDVPT 245 Query: 242 REQRVYPRRYLSKWGAGIAWRRSTAYRFLLTKSFARGDIFGIVIRIFLLALLLVWWNRES 301 R PR YL+ I R Y +L S R +I GIV+R+ +L L+V+W E Sbjct: 246 MPSRTAPRSYLAWIIRYIPNRHRLTYAYLYAASLVRTEIGGIVLRLTVLFALIVYWFAED 305 Query: 302 YIGSG-----IYLFFVLVMGVQLSALRKLHSESFWLTVYPLPEGSKASSTI 347 SG Y+ + ++ VQL LR H S W VYPLPE + S + Sbjct: 306 GWLSGWGAAAAYMILLQLVAVQLGGLRHAHRYSVWRHVYPLPEAKRMESLV 356 >A8FBL9.1 A8FBL9_BACP2 ABC superfamily ATP binding cassette transporter, membrane protein Length = 406 Score = 95.5 bits (236), Expect = 9e-18, Method: Compositional matrix adjust. Identities = 80/341 (23%), Positives = 147/341 (43%), Gaps = 3/341 (0%) Query: 8 RQRRSRFMGGLTPYVGYIIQSGIAMVFLLVLIIFSAWYTSLLRDVPEGIPIRWIMLVLLL 67 + R + Y+ Y+ + +V + L ++WY++ L+ +P P W+M V+ Sbjct: 10 KSRLEEHIQDTRKYLKYMFNDHLVIVLIFFLAGGASWYSNWLKQIPAHFPSYWVMAVVFS 69 Query: 68 PAAAHSSFRTYLQSPDTIFLLPQGHRMKEYFAPAWTAGNVWKILRLAFVLITLWPLY-IR 126 S RT ++ D +FLLP RM+ Y A+ + ++ L V I PLY Sbjct: 70 LVLTSSYVRTLIKEADLVFLLPLEGRMEPYLKQAFNFSFISQLFPLIAVSIVALPLYSAV 129 Query: 127 TDDSHQMLFATLAVLILVKILASFGLWRELAMLSRPASMGYSLLRWAVSGLMIAAWLWQP 186 + D +M LA +IL+K L + WR + ++LR+ ++ ++I L Sbjct: 130 SSDGIKMYVLILAQMILIKGLNTAIQWRMTYFQGKQMRWLDAVLRFLLNMILIYTVLQAS 189 Query: 187 XXXXXXXXXXXXXXXXXXXXXXXXXSVPWERLIATERNQGARALMILGWFVDVPGREQRV 246 WE I+ E + R + F DVP +++V Sbjct: 190 YAIAIVFYLIYGVYLVYLTNAVKKQPFQWELHISDELKRKQRFYRLANLFTDVPHLKKQV 249 Query: 247 YPRRYLSKWGAGIAWRRSTAYRFLLTKSFAR-GDIFGIVIRIF-LLALLLVWWNRESYIG 304 R Y+ + + + + ++ ++F R D FG+VIR+ + L++++ ++G Sbjct: 250 KRRIYMDWLLQFVPYDQRKTFSYMYVRAFLRSNDYFGLVIRLTAVFVLIILYTGAAGWVG 309 Query: 305 SGIYLFFVLVMGVQLSALRKLHSESFWLTVYPLPEGSKASS 345 + + LF V + GVQL L K + +YP+ + K S Sbjct: 310 ALLVLFTVFITGVQLVPLTKHFAHLSLQALYPVAQQEKDRS 350 >C2URD8.1 C2URD8_BACCE ABC transporter EcsB Length = 399 Score = 90.1 bits (222), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 74/356 (20%), Positives = 156/356 (43%), Gaps = 12/356 (3%) Query: 1 MDLKELRRQRRSRFMGGLTPYVGYIIQSGIAMVFLLVLIIFSAWYTSLLRDVP-EGIPIR 59 M ++ L R + M + Y +I +G+ F+ + + + +Y L+ P +GI + Sbjct: 1 MSIESLWSSRFQQHMQNIITYFARMI-NGLLYSFIFISCVGAYYYAQFLKASPSKGISLL 59 Query: 60 WIMLVLLLPAAAHSSFRTYLQSPDTIFLLPQGHRMKEYFAPAWTAGNVWKILRLAFVLIT 119 I +VL RT++Q PD ++LL ++ YF + + ++ L F + Sbjct: 60 LITIVLTF-IITRCPIRTFIQKPDAVYLLALEEKLTSYFKKSLLYNYIIQLFPLLFTFLI 118 Query: 120 LWPLYIRTDDSHQMLFATLAVLILVKI---LASFGLWRELAMLSRPASMGYSLLRWAVSG 176 L PL +++ T+ +++++ + + +WR+ S ++ + ++R A + Sbjct: 119 LVPLAMQSLQLTVPFLCTIFIVLMITKAWNMYIYWMWRD----SHEKNIWF-IIRIACNA 173 Query: 177 LMIAAWLWQPXXXXXXXXXXXXXXXXXXXXXXXXXSVPWERLIATERNQGARALMILGWF 236 L+I + +PW+ +I E R F Sbjct: 174 LIIYMLFYSAHVLILGGLLLLLAFLLLYTKKQPTKRIPWDYIIEQEEKMNVRFYQFASIF 233 Query: 237 VDVPGREQRVYPRRYLSKWGAGIAWRRSTAYRFLLTKSFARG-DIFGIVIRIFLLALLLV 295 DVP ++V R++L+ W + ++ + +L T +F RG D FGI IR+ L+ + Sbjct: 234 TDVPQLNKQVNKRKWLTNWIEPLLHKKRETFFYLHTLAFLRGNDYFGIYIRLGLIGAFAL 293 Query: 296 WWNRESYIGSGIYLFFVLVMGVQLSALRKLHSESFWLTVYPLPEGSKASSTIQFIF 351 ++ +Y+ I + ++ +QL +L K S + + +YP+ + + ++ IF Sbjct: 294 YFIPNTYVKGAIAYIVLYMISMQLRSLWKYFSGNIIVALYPIDTEKRMNQFLRLIF 349 >C3BY76.1 C3BY76_BACTU ABC transporter EcsB Length = 399 Score = 89.4 bits (220), Expect = 6e-16, Method: Compositional matrix adjust. Identities = 75/356 (21%), Positives = 154/356 (43%), Gaps = 12/356 (3%) Query: 1 MDLKELRRQRRSRFMGGLTPYVGYIIQSGIAMVFLLVLIIFSAWYTSLLRDVP-EGIPIR 59 M ++ L R + M + Y +I +G+ F+ + + + +Y L+ P +GI + Sbjct: 1 MSIESLWSSRFQQHMQNIITYFARMI-NGLLYSFIFISCVGAYYYAQFLKASPSKGISLL 59 Query: 60 WIMLVLLLPAAAHSSFRTYLQSPDTIFLLPQGHRMKEYFAPAWTAGNVWKILRLAFVLIT 119 I +VL RT++Q PD ++LL ++ YF + + ++ L F + Sbjct: 60 LITIVLTF-IITRCPIRTFIQKPDAVYLLALEEKLTSYFKKSLLYNYIIQLFPLLFTFLI 118 Query: 120 LWPLYIRTDDSHQMLFATLAVLILVKI---LASFGLWRELAMLSRPASMGYSLLRWAVSG 176 L PL +++ T+ +++++ + +WR+ S S+ +S++R + Sbjct: 119 LVPLALQSLQVTVPFLCTIFIVLMITKAWNMYIHWMWRD----SHEKSI-WSIIRITCNA 173 Query: 177 LMIAAWLWQPXXXXXXXXXXXXXXXXXXXXXXXXXSVPWERLIATERNQGARALMILGWF 236 L+I + +PW+ +I E R F Sbjct: 174 LIIYMLFYSANVLILGGLLLLLAFLLLYTKKQPTKRIPWDYIIEQEEKMNVRFYQFASIF 233 Query: 237 VDVPGREQRVYPRRYLSKWGAGIAWRRSTAYRFLLTKSFARG-DIFGIVIRIFLLALLLV 295 DVP ++V R++L+ W + ++ + +L T +F RG D FGI IR+ L+ + + Sbjct: 234 TDVPQLNKQVNKRKWLTTWIEPLLHKKRATFFYLHTLAFLRGNDYFGIYIRLGLIGVFAL 293 Query: 296 WWNRESYIGSGIYLFFVLVMGVQLSALRKLHSESFWLTVYPLPEGSKASSTIQFIF 351 ++ Y+ I + ++ +QL +L K S + + +YP+ + + + IF Sbjct: 294 YFIPNIYVKGAIAYIVLYMISMQLRSLWKYFSGNIIVALYPIDTEKRMNQFLHLIF 349 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.330 0.142 0.457 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,161,826,583 Number of extensions: 80921025 Number of successful extensions: 251047 Number of sequences better than 1.0e-05: 221 Number of HSP's gapped: 250828 Number of HSP's successfully gapped: 240 Length of query: 405 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 267 Effective length of database: 1,760,596,133 Effective search space: 470079167511 Effective search space used: 470079167511 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)