BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0389.0004.p (273 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KEG5.1 B3KEG5_9BACI Phosphatidylserine decarboxylase 363 6e-99 B1D7A2.1 B1D7A2_9BACL Phosphatidylserine decarboxylase 318 4e-85 B0TC31.1 B0TC31_HELMI Phosphatidylserine decarboxylase 224 6e-57 B4BLX1.1 B4BLX1_9BACI Phosphatidylserine decarboxylase 216 1e-54 B7Y5T6.1 B7Y5T6_9BACI Phosphatidylserine decarboxylase 215 3e-54 >B3KEG5.1 B3KEG5_9BACI Phosphatidylserine decarboxylase Length = 267 Score = 363 bits (933), Expect = 6e-99, Method: Compositional matrix adjust. Identities = 180/259 (69%), Positives = 207/259 (79%) Query: 11 MVKQLLRLMTELSSHRWLSRLMGAFSHSRLSRFLIPAFIRIYHIPASEAEKHSGEYLTLN 70 M KQLLRLMTELSS +WLSR+MG SHSRLSR LIPAFIRIY IPA EAEK EY +LN Sbjct: 1 MAKQLLRLMTELSSRKWLSRIMGHCSHSRLSRHLIPAFIRIYQIPAHEAEKEIHEYRSLN 60 Query: 71 EFFSRRLKPGMRPIASHENAVASPVDALITAMGEIHSGTIMNVKGQDYELEDLLNHSPHL 130 EFFSRRLK G+R I +A+ SPVDA I+AM +++GTI++VKGQDY L +LL SP + Sbjct: 61 EFFSRRLKIGLRAIDDTADAMVSPVDATISAMCAVNAGTILSVKGQDYTLSELLAKSPRM 120 Query: 131 ELYKKGFFFVLYLSPTDYHRIHSPLTGRKVESEHIRGRAYPVNEFGMRHMKGVLSRNERL 190 E Y G+ FVLYLSPTDYHRIHSP+TG + ESEH++GR YPVN+F M H++ VLSRNERL Sbjct: 121 ENYINGYAFVLYLSPTDYHRIHSPVTGIQAESEHLKGRVYPVNDFAMTHIRNVLSRNERL 180 Query: 191 ITYIAGANGEAAVVKVGAMNVSSIHYADETADAWQIGDDLAYFEFGSTVVLLLENGSFTA 250 ITYIA GE AVVKVGAMNVSSI YADE+ + IGD+LAYFEFGSTVVLL ENG+FT Sbjct: 181 ITYIAHEFGEVAVVKVGAMNVSSIRYADESVKKYDIGDELAYFEFGSTVVLLTENGTFTP 240 Query: 251 RPGLTSGTKVKMGELLGTL 269 R L G+KVKMGELLG L Sbjct: 241 RGDLEPGSKVKMGELLGLL 259 >B1D7A2.1 B1D7A2_9BACL Phosphatidylserine decarboxylase Length = 262 Score = 318 bits (815), Expect = 4e-85, Method: Compositional matrix adjust. Identities = 158/259 (61%), Positives = 189/259 (72%) Query: 11 MVKQLLRLMTELSSHRWLSRLMGAFSHSRLSRFLIPAFIRIYHIPASEAEKHSGEYLTLN 70 M+ L + MTELSS +W+SRL G F+ S+ SR IP F +Y+I EAEK Y TLN Sbjct: 1 MMGWLFQAMTELSSRKWISRLTGRFAQSKASRGWIPRFASMYNIKIEEAEKPLEAYSTLN 60 Query: 71 EFFSRRLKPGMRPIASHENAVASPVDALITAMGEIHSGTIMNVKGQDYELEDLLNHSPHL 130 EFF+RRLKPG R I A+ SPVDALIT G I +GTI+NVKGQDY +E+LLN SP Sbjct: 61 EFFTRRLKPGSRIIHEEATALISPVDALITGAGPIKAGTIVNVKGQDYTIEELLNRSPRT 120 Query: 131 ELYKKGFFFVLYLSPTDYHRIHSPLTGRKVESEHIRGRAYPVNEFGMRHMKGVLSRNERL 190 ELY+ G++ VLYLSPTDYHRIH+P+ G+ VE EH+ G+ YPVN+FG+R M+ VLSRNERL Sbjct: 121 ELYRDGYYIVLYLSPTDYHRIHTPVDGQIVEREHVPGKVYPVNDFGLRRMRRVLSRNERL 180 Query: 191 ITYIAGANGEAAVVKVGAMNVSSIHYADETADAWQIGDDLAYFEFGSTVVLLLENGSFTA 250 ITYI A GE AVVKVGAMNVSSI Y + D G +LAYFEFGSTVVLL+EN +F Sbjct: 181 ITYIKHAGGETAVVKVGAMNVSSIRYVEPIRDKAVKGGELAYFEFGSTVVLLMENDTFIP 240 Query: 251 RPGLTSGTKVKMGELLGTL 269 R L G KV+MGE+LG L Sbjct: 241 RQDLAPGLKVRMGEMLGKL 259 >B0TC31.1 B0TC31_HELMI Phosphatidylserine decarboxylase Length = 274 Score = 224 bits (571), Expect = 6e-57, Method: Compositional matrix adjust. Identities = 117/254 (46%), Positives = 155/254 (61%), Gaps = 14/254 (5%) Query: 27 WLSRLMGAFSHSRLSRFLIPAFIRIYHIPASEAEKHSGEYLTLNEFFSRRLKPGMRPIAS 86 WLSR G ++ SR SR IP FIR Y + EAEK EY +L +FF RRLKPGMRPI Sbjct: 16 WLSRQSGKWAASRWSRRAIPWFIRRYGVNLEEAEKSWQEYRSLADFFCRRLKPGMRPICP 75 Query: 87 HENAVASPVDALITAMGEIHSGTIMNVKGQDYELEDLLNHSPHLELYKKGFFFVLYLSPT 146 E+ + SPVD ++ +G +G ++ KG +Y LE LL + + G F +YLSP Sbjct: 76 DESVIISPVDGKVSQIGTASAGRLIQAKGINYSLEQLLGDAEQARRFTGGEFITIYLSPR 135 Query: 147 DYHRIHSPLTGRKVESEHIRGRAYPVNEFGMRHMKGVLSRNERLITYIAGANGEAAVVKV 206 DYHRIH+P+ GR + GR YPVNE G+R + + +RNERLITY+ G+ AV+KV Sbjct: 136 DYHRIHAPMAGRVTGYAYWPGRLYPVNELGVRGVPNLFARNERLITYMKTDVGQVAVIKV 195 Query: 207 GAMNVSSIH--YAD----------ETADAWQI--GDDLAYFEFGSTVVLLLENGSFTARP 252 GAM V S+ YA+ D + GD+L YFEFGSTV+LL E G+ +P Sbjct: 196 GAMMVGSVRVGYAEINRRKRAKLISMTDGPYLDKGDELGYFEFGSTVILLYEPGAIRWKP 255 Query: 253 GLTSGTKVKMGELL 266 G+ +GT++KMGE L Sbjct: 256 GIETGTRLKMGEGL 269 >B4BLX1.1 B4BLX1_9BACI Phosphatidylserine decarboxylase Length = 259 Score = 216 bits (551), Expect = 1e-54, Method: Compositional matrix adjust. Identities = 115/258 (44%), Positives = 162/258 (62%), Gaps = 3/258 (1%) Query: 11 MVKQLLRLMTELSSHRWLSRLMGAFSHSRLSRFLIPAFIRIYHIPASEAEKHSGEYLTLN 70 M K L RL EL++H S+L+ +F+ SRLS LIP++ +IYHI E EK Y TL Sbjct: 1 MRKWLYRLFIELTNHSLSSKLLASFARSRLSALLIPSYAKIYHINQEEMEKSLKNYKTLQ 60 Query: 71 EFFSRRLKPGMRPIASHENAVASPVDALITAMGEIHSGTIMNVKGQDYELEDLLNHSPHL 130 + F RRLK G RP+ + E+A+ SPVDA+I MG I + + VKG+ Y + ++L + Sbjct: 61 QLFVRRLKAGTRPVDADEHAIVSPVDAIIEEMGTIQENSEIIVKGKPYSIAEMLGSAEAA 120 Query: 131 ELYKKGFFFVLYLSPTDYHRIHSPLTGRKVESEHIRGR-AYPVNEFGMRHMKGVLSRNER 189 + Y GFFF+LYLSP+ YHRIHSP++G +E + GR +YPVN G+++ + L +N R Sbjct: 121 QPYVNGFFFILYLSPSHYHRIHSPISG-VIEKQWSLGRKSYPVNRLGLKYGRRPLEKNYR 179 Query: 190 LITYIAGANGEAAVVKVGAMNVSSIHYADETADAWQIGDDLAYFEFGSTVVLLLENGSFT 249 LIT + A+VK+GAM V+SI + + G+++AYF FGSTVVLL E GSF Sbjct: 180 LITEVTAGGKRMAIVKIGAMFVNSIELTHKEERLVK-GEEMAYFSFGSTVVLLFEQGSFA 238 Query: 250 ARPGLTSGTKVKMGELLG 267 P + +K+GE LG Sbjct: 239 PDPRIHPPMPIKVGERLG 256 >B7Y5T6.1 B7Y5T6_9BACI Phosphatidylserine decarboxylase Length = 264 Score = 215 bits (548), Expect = 3e-54, Method: Compositional matrix adjust. Identities = 116/258 (44%), Positives = 160/258 (62%), Gaps = 3/258 (1%) Query: 11 MVKQLLRLMTELSSHRWLSRLMGAFSHSRLSRFLIPAFIRIYHIPASEAEKHSGEYLTLN 70 M K L RL EL++H S+L+ +F+ SRLS LI ++ +IYHI E EK Y TL Sbjct: 1 MRKWLYRLFIELTNHSLSSKLLASFAKSRLSGLLISSYAKIYHINQDEMEKSLKNYKTLQ 60 Query: 71 EFFSRRLKPGMRPIASHENAVASPVDALITAMGEIHSGTIMNVKGQDYELEDLLNHSPHL 130 + F RRLK G+RP+ + E+ V SPVDA+I MG I M VKG+ Y + ++L Sbjct: 61 QLFVRRLKAGVRPVDADEHTVVSPVDAVIEDMGTIRENCEMIVKGKPYSIAEMLGSVEAA 120 Query: 131 ELYKKGFFFVLYLSPTDYHRIHSPLTGRKVESEHIRGR-AYPVNEFGMRHMKGVLSRNER 189 + Y GFFF+LYLSP+ YHRIHSP++G +E + GR +YPVN G+++ + L +N R Sbjct: 121 QPYVNGFFFILYLSPSHYHRIHSPISG-VIEKQWALGRKSYPVNRLGLKYGRRPLEKNYR 179 Query: 190 LITYIAGANGEAAVVKVGAMNVSSIHYADETADAWQIGDDLAYFEFGSTVVLLLENGSFT 249 LIT + A+VK+GAM V+SI E + G+++AYF FGSTVVLL E GSF Sbjct: 180 LITEVTAGGKRLAIVKIGAMFVNSIELTHEGEQLVK-GEEMAYFSFGSTVVLLFERGSFA 238 Query: 250 ARPGLTSGTKVKMGELLG 267 P + + +K+GE LG Sbjct: 239 PDPRIVAPMPIKVGERLG 256 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.137 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,604,882,026 Number of extensions: 66317934 Number of successful extensions: 149682 Number of sequences better than 1.0e-05: 993 Number of HSP's gapped: 147945 Number of HSP's successfully gapped: 1027 Length of query: 273 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 139 Effective length of database: 1,798,280,193 Effective search space: 249960946827 Effective search space used: 249960946827 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)