BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0386.0034.p (297 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KDA9.1 B3KDA9_9BACI Putative uncharacterized protein 391 e-107 Q65IY2.1 Q65IY2_BACLD SAM dependent methyltranferase 343 1e-92 B9CPE8.1 B9CPE8_STACP SAM dependent methyltranferase 162 3e-38 Q0AXM9.1 Q0AXM9_SYNWW Putative uncharacterized protein 129 3e-28 C0QMC2.1 C0QMC2_DESAH Putative SAM-dependent methyltransferase 113 2e-23 >B3KDA9.1 B3KDA9_9BACI Putative uncharacterized protein Length = 301 Score = 391 bits (1005), Expect = e-107, Method: Compositional matrix adjust. Identities = 183/293 (62%), Positives = 233/293 (79%), Gaps = 2/293 (0%) Query: 3 DDFFNAAMWEKVWKDESDLKIDRMKKSGIDPTRSFDHKAKVFNEQSFNEKGRQRTKRIIS 62 +DFFN A+WE+ WK+ D I++MKK+GI+P R+FDHKA+ FNEQSFNE+GR+RT RI++ Sbjct: 2 NDFFNEAIWEQAWKEHGDSVINKMKKAGIEPARAFDHKARTFNEQSFNEEGRKRTSRIMN 61 Query: 63 WLEGQGVQFENASVLDIGAASGVFSVPFAEKGARVTAVETSPPLIELLEENVANFAEGQV 122 WLEGQGV+F+NASVLDIGAASGVFSVPFAE+GA VTAVE+S PLI+LL+EN+ NF + QV Sbjct: 62 WLEGQGVRFDNASVLDIGAASGVFSVPFAERGADVTAVESSLPLIDLLKENITNFPQDQV 121 Query: 123 KIVPEPFEDIDVASMGWNQTFDLVFASMIPIIHNWESVEKILQCARKFCYISMPVLHTEH 182 KIVPE FEDIDV + GW+Q FDLVF SM P+I +W+SVEK+LQCAR+FCYISMP TE Sbjct: 122 KIVPERFEDIDVQAKGWSQAFDLVFLSMCPVIVDWQSVEKVLQCAREFCYISMPAGSTEI 181 Query: 183 SLVKEIWPLVTGQSYQSKLTEMGXXXXXXXXXXXXXETLVTAEGKTREIPGEMALQEVMT 242 SL+ EIWPL+T Q Y+SK E G E+LVT E KT E+ E ALQE+M Sbjct: 182 SLMNEIWPLITDQPYRSKHMEFGYLLHLLYLKGYSYESLVTRETKTTEVSSEAALQEIMN 241 Query: 243 WL-GHHRLPADERNMRIVANYLEQSYPSGKVVVQEGGRYGKVLIRLQDQYMYT 294 WL GH ++P D++ +++ +YLEQ+YPSGKV +++ GRYGKVL+RLQD+ MY+ Sbjct: 242 WLRGHGQVPDDQKR-KLITDYLEQAYPSGKVTIRQSGRYGKVLVRLQDESMYS 293 >Q65IY2.1 Q65IY2_BACLD SAM dependent methyltranferase Length = 296 Score = 343 bits (880), Expect = 1e-92, Method: Compositional matrix adjust. Identities = 160/293 (54%), Positives = 212/293 (72%) Query: 1 MNDDFFNAAMWEKVWKDESDLKIDRMKKSGIDPTRSFDHKAKVFNEQSFNEKGRQRTKRI 60 M ++FF +WE+ WK++ +++MKK+G+D FD +AK FNEQ+FNE+GR+RTKRI Sbjct: 1 MINEFFKETVWEEAWKEDQQTAVNKMKKAGMDKVHHFDLRAKSFNEQAFNEEGRKRTKRI 60 Query: 61 ISWLEGQGVQFENASVLDIGAASGVFSVPFAEKGARVTAVETSPPLIELLEENVANFAEG 120 + WLEGQGV F++ S+LDIGAASG F+VPFAE+GA VTAVE S PL+EL++EN+ G Sbjct: 61 MKWLEGQGVTFKDTSILDIGAASGGFTVPFAERGAEVTAVEPSLPLVELMKENITGITNG 120 Query: 121 QVKIVPEPFEDIDVASMGWNQTFDLVFASMIPIIHNWESVEKILQCARKFCYISMPVLHT 180 +VKIV EPFEDID+ + GW FD VF SM P I +WESV+++L CAR+FCYIS+ Sbjct: 121 KVKIVAEPFEDIDIQAKGWGNAFDFVFVSMCPAIADWESVKRVLSCARQFCYISLSAGSR 180 Query: 181 EHSLVKEIWPLVTGQSYQSKLTEMGXXXXXXXXXXXXXETLVTAEGKTREIPGEMALQEV 240 EHSL+ EIWPLV+ Q +++ +EM E+LVT E KTRE+ E AL EV Sbjct: 181 EHSLLNEIWPLVSDQPLKNEHSEMAYLLHLLFLKGYTYESLVTREMKTREVSRETALNEV 240 Query: 241 MTWLGHHRLPADERNMRIVANYLEQSYPSGKVVVQEGGRYGKVLIRLQDQYMY 293 M L LPAD+RN +I A YLE++YP+ KV +++GGR+GKVLIRLQ+Q MY Sbjct: 241 MDMLRMFGLPADDRNRKITAAYLERTYPNDKVEIRQGGRFGKVLIRLQNQNMY 293 >B9CPE8.1 B9CPE8_STACP SAM dependent methyltranferase Length = 286 Score = 162 bits (410), Expect = 3e-38, Method: Compositional matrix adjust. Identities = 93/291 (31%), Positives = 167/291 (57%), Gaps = 10/291 (3%) Query: 2 NDDFFNAAMWEKVWKDESDLKIDRMKKSGIDPTRS--FDHKAKVFNEQSFNEKGRQRTKR 59 N++ F+ W++ W ++ + + RMK++G+ + F+ A+ FN+ SF E+ ++R R Sbjct: 3 NENIFDQQFWKEAWDNDPNTQDKRMKRAGLGQPDAPGFEKWAENFNKNSFTEESQKRVNR 62 Query: 60 IISWLEGQGVQFENASVLDIGAASGVFSVPFAEKGARVTAVETSPPLIELLEENVANFAE 119 I+SW++ Q FEN S+LD+GAASGVFS+PF+++ A+VT++E S L ++L++N ++ Sbjct: 63 IMSWIQNQTGGFENLSILDVGAASGVFSIPFSKESAKVTSLEPSSILHDMLKDNAQHYG- 121 Query: 120 GQVKIVPEPFEDIDVASMGWNQTFDLVFASMIPIIHNWESVEKILQCARKFCYISMPVLH 179 +++ + E FE+I+ + + FDLVFASM P + WE+VEK + A ++ Y+S+ Sbjct: 122 VEIETINESFENIETTDI---KKFDLVFASMCPAVTTWEAVEKAIDIAHQYVYVSLIAGP 178 Query: 180 TEHSLVKEIWPLVTGQSYQSKLT-EMGXXXXXXXXXXXXXETLVTAEGKTREIPGEMALQ 238 E+S+V E+ + + S +T ++ ETL+ + E + ++ Sbjct: 179 KENSIVDEVVAFLELEP--SPMTADLYYLLQLLYCNNYTYETLIERHTQHAEKSVDEVMR 236 Query: 239 EVMTWLGHHRLPADERNMRIVANYLEQSYPSGKVVVQEGGRYGKVLIRLQD 289 ++ +WL + + + + YL Y V V GG++GKVLI LQD Sbjct: 237 QLPSWLKEVEIEMNAQQKESIEQYLRDKY-GNHVPVVTGGKFGKVLITLQD 286 >Q0AXM9.1 Q0AXM9_SYNWW Putative uncharacterized protein Length = 286 Score = 129 bits (324), Expect = 3e-28, Method: Compositional matrix adjust. Identities = 69/203 (33%), Positives = 116/203 (57%), Gaps = 2/203 (0%) Query: 3 DDFFNAAMWEKVWKDESDLKIDRMKKSGIDPTRSFDHKAKVFNEQSFNEKGRQ-RTKRII 61 D+ F WE+ W E K +R + ++ +A+ F + RQ RT+ + Sbjct: 2 DELFIPEFWERKWI-EFKSKNNRYRGQCKTAGEYWNQRAEGFANSTIKSDSRQERTREVF 60 Query: 62 SWLEGQGVQFENASVLDIGAASGVFSVPFAEKGARVTAVETSPPLIELLEENVANFAEGQ 121 S+LE G+ + +VLDIG+ G F++P AE+GARV A++ + ++E+L+EN+ G Sbjct: 61 SFLELFGIIKSDMTVLDIGSGPGSFAIPLAERGARVVALDPASRMLEILQENLPPSMPGS 120 Query: 122 VKIVPEPFEDIDVASMGWNQTFDLVFASMIPIIHNWESVEKILQCARKFCYISMPVLHTE 181 V V +ED DV + GW FDLVFASM P ++N E+++K++ C+R++CY+S Sbjct: 121 VNCVQALWEDYDVENRGWENKFDLVFASMSPGVNNLETLQKMMFCSRRWCYLSGFSGTRN 180 Query: 182 HSLVKEIWPLVTGQSYQSKLTEM 204 +SL E++ +TG Y + ++ Sbjct: 181 YSLYNEVFKQITGGPYINHFNDI 203 >C0QMC2.1 C0QMC2_DESAH Putative SAM-dependent methyltransferase Length = 288 Score = 113 bits (283), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 65/195 (33%), Positives = 108/195 (55%), Gaps = 4/195 (2%) Query: 7 NAAMWEKVWKDESDLKIDRMKKSGIDPTRSFDHKAKVFNEQSFNEKGRQRTKRIISWLEG 66 N +W+ +W+ E LK D K D + +D +A F++++ + + R +RI+S LE Sbjct: 8 NQTVWKDLWQKE--LKNDPETKKSQDHIKKWDQRAPSFDKRTVSPEAVLRKERILSMLED 65 Query: 67 QGVQFENASVLDIGAASGVFSVPFAEKGARVTAVETSPPLIELLEENVA--NFAEGQVKI 124 G +LDIG+ G +++P E GA V AVE S ++++L+E +A Q++I Sbjct: 66 AGALQAGNRILDIGSGPGNWAIPMVEMGAEVVAVEPSGGMVKILKEKMAAKGIHSDQLRI 125 Query: 125 VPEPFEDIDVASMGWNQTFDLVFASMIPIIHNWESVEKILQCARKFCYISMPVLHTEHSL 184 ++D+DV G + FDLVFASM P + + E++ K ++ +RKFCY+S S Sbjct: 126 DQRAWQDVDVEKEGLSGQFDLVFASMSPGVRDPETLNKAMKASRKFCYLSTFSGGGWRSC 185 Query: 185 VKEIWPLVTGQSYQS 199 +W +TGQ +S Sbjct: 186 YNNLWQSITGQVLES 200 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.133 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,706,005,944 Number of extensions: 64623362 Number of successful extensions: 168898 Number of sequences better than 1.0e-05: 68 Number of HSP's gapped: 168941 Number of HSP's successfully gapped: 68 Length of query: 297 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 162 Effective length of database: 1,788,859,178 Effective search space: 289795186836 Effective search space used: 289795186836 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)