BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0386.0033.p (477 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DCM1.1 B1DCM1_9BACL Drug resistance transporter, EmrB/QacA sub... 577 e-162 B3K445.1 B3K445_9BACI Drug resistance transporter, EmrB/QacA sub... 565 e-159 C2WE71.1 C2WE71_BACCE Lincomycin resistance protein 494 e-137 C2V806.1 C2V806_BACCE Lincomycin resistance protein 486 e-135 C2PBD6.1 C2PBD6_BACCE Lincomycin resistance protein lmrB 474 e-132 >B1DCM1.1 B1DCM1_9BACL Drug resistance transporter, EmrB/QacA subfamily Length = 497 Score = 577 bits (1486), Expect = e-162, Method: Compositional matrix adjust. Identities = 296/475 (62%), Positives = 371/475 (78%) Query: 3 DLNNIRRGPIMLALIIGMFVAALNETLMGNALPALMQSFDASAATVQWLSTAYMLVVGVL 62 DL NI+RGPI+ +IIG F+A LNETL+GNALP LM++F+ +T+QWL+TAYMLVVGVL Sbjct: 14 DLTNIKRGPIIATIIIGAFIAILNETLLGNALPELMKAFEVGPSTIQWLTTAYMLVVGVL 73 Query: 63 VPITAILQQWFTTRQLFLSAMGLFFIGTVISAVSPVFSVLLIGRIIQAVGTGMLLPLVMN 122 VP+TA+LQQWFTTRQ+F+ AM LFF GTVISAV+P F VLL+GRI+QA+GTG++LP++MN Sbjct: 74 VPVTALLQQWFTTRQMFIGAMTLFFAGTVISAVAPEFGVLLVGRIVQAIGTGLMLPVLMN 133 Query: 123 VIMTIYPPEKRXXXXXXXXXXXXFAPAIGPTISGLIVDGLSWRWLFYLVIPFALISIFVG 182 IM I+PPEKR APAIGPT+SG+I+D L+WRWLF LVIP A +SI Sbjct: 134 TIMVIFPPEKRGGAMGLIGLVIMAAPAIGPTLSGVIMDSLNWRWLFILVIPLAALSIIFS 193 Query: 183 AAVLKNVSEVTKPRIDFLSIVLSTIGFGGIVYGFSSAGEHGWAVPEVIWTIAVGGVALLL 242 LKNVS++TKP++D +SI+LSTIGFGG+VYGFSS GE GW+ P VIWTIAVG VAL++ Sbjct: 194 FMFLKNVSDITKPKVDIISILLSTIGFGGLVYGFSSTGEKGWSDPVVIWTIAVGAVALII 253 Query: 243 FVWRQLKLEQPIMDLRAFRYPXXXXXXXXXXXXXXXXXSSAIMLPMFMQGVLLITAFKSG 302 F+ RQL+LE PI+DLRAF+ P S+ +LPMF+QG LL+TAF SG Sbjct: 254 FILRQLRLEAPILDLRAFKSPMFSIVTILLFVLMMTLFSTMTLLPMFLQGALLMTAFTSG 313 Query: 303 LILLPGGIVNGVMAPISGKLFDKFGPRILIIPGLTIAVISLWQFTRFNEATSTGFIVSVH 362 +I+LPG IVNG+MAPISG LFDKFGPRIL+ PGL + ISLW FT + S G I+ +H Sbjct: 314 IIMLPGSIVNGIMAPISGVLFDKFGPRILVTPGLILVAISLWMFTGIDATASKGHIIMIH 373 Query: 363 IALMIGIALVMMPAQTTGLNQLPRNLHPHGTAILNTLQQVAGAIGTALYISIMSGGQKQY 422 I +M+GI+LVMMPAQT+GLNQLPR+L+PHGTAILNTLQQV+GAIGTAL+ISIMS G K Y Sbjct: 374 IIMMVGISLVMMPAQTSGLNQLPRHLYPHGTAILNTLQQVSGAIGTALFISIMSNGTKDY 433 Query: 423 LSGVSDPSASSEITKGLAAGINNAFWFGVIVALIALGIGLFLRKGKSPEHEQLAE 477 L SDP + +E G+ AGI++AFW G+IVA+ A+ IGLF+++ K PE +Q AE Sbjct: 434 LKTSSDPGSPAEQAHGMVAGIHDAFWIGLIVAIAAIIIGLFVKRTKPPEGDQGAE 488 >B3K445.1 B3K445_9BACI Drug resistance transporter, EmrB/QacA subfamily Length = 484 Score = 565 bits (1457), Expect = e-159, Method: Compositional matrix adjust. Identities = 271/473 (57%), Positives = 361/473 (76%), Gaps = 4/473 (0%) Query: 2 IDLNNIRRGPIMLALIIGMFVAALNETLMGNALPALMQSFDASAATVQWLSTAYMLVVGV 61 IDL+ I+RGPI+ ALIIG FV+ LNETL+ A P+LM+ F +TVQWLST+YMLVVG+ Sbjct: 12 IDLSKIKRGPIVAALIIGAFVSILNETLLNIAFPSLMEQFGIGESTVQWLSTSYMLVVGI 71 Query: 62 LVPITAILQQWFTTRQLFLSAMGLFFIGTVISAVSPVFSVLLIGRIIQAVGTGMLLPLVM 121 LVP+TA+LQ WFTTRQ+F+ AM LF IGT+I SPVF VLL+GRI+QAVGTG++LP++M Sbjct: 72 LVPVTALLQLWFTTRQMFIGAMSLFLIGTIICGFSPVFGVLLVGRILQAVGTGLMLPVLM 131 Query: 122 NVIMTIYPPEKRXXXXXXXXXXXXFAPAIGPTISGLIVDGLSWRWLFYLVIPFALISIFV 181 N I++IYPP+ R APAIGPT+SGLIVD L+WRWLFYLVIP A SI Sbjct: 132 NTILSIYPPQNRGAAMGMIGLVIMTAPAIGPTLSGLIVDALNWRWLFYLVIPLAAFSIVF 191 Query: 182 GAAVLKNVSEVTKPRIDFLSIVLSTIGFGGIVYGFSSAGEHGWAVPEVIWTIAVGGVALL 241 A LKNVSE+TKP++D++SI+LSTIGFGGIVYGFSSAGE W+ P+VIW I G ++LL Sbjct: 192 AAVFLKNVSELTKPKVDYISIILSTIGFGGIVYGFSSAGEGTWSDPKVIWCITAGAISLL 251 Query: 242 LFVWRQLKLEQPIMDLRAFRYPXXXXXXXXXXXXXXXXXSSAIMLPMFMQGVLLITAFKS 301 LFVWRQLK+E+P++DLR F++P S+ IMLP+F+Q L +TA + Sbjct: 252 LFVWRQLKMEEPMLDLRTFKFPMFSLVTVLMLVLMMSMFSTMIMLPLFLQNALGLTALSA 311 Query: 302 GLILLPGGIVNGVMAPISGKLFDKFGPRILIIPGLTIAVISLWQFTRFNEATSTGFIVSV 361 GL+++PGGI+NG+MAPI+GKLFDKFGPR+LIIPGL + I+++ F+R + + + G+++ + Sbjct: 312 GLVMMPGGIINGLMAPITGKLFDKFGPRVLIIPGLALLSIAIFLFSRIDASWTGGYVIFL 371 Query: 362 HIALMIGIALVMMPAQTTGLNQLPRNLHPHGTAILNTLQQVAGAIGTALYISIMSGGQKQ 421 H+ +MIGI++VMMPAQTT LNQLPR L+PHGTAI+NTLQQVAGA+G AL+ISIM+ G K Sbjct: 372 HVMMMIGISMVMMPAQTTALNQLPRLLYPHGTAIMNTLQQVAGAVGMALFISIMTAGAKN 431 Query: 422 YLSGVSDPSASSEITKGLAAGINNAFWFGVIVALIALGIGLFLRKGKSPEHEQ 474 Y+ D + + + AG++NAF G+++A+IA +GLF+++ SP E+ Sbjct: 432 YVETTGDLNP----VQSMVAGLHNAFVIGLVLAIIAFVLGLFVKRTSSPVEEK 480 >C2WE71.1 C2WE71_BACCE Lincomycin resistance protein Length = 491 Score = 494 bits (1271), Expect = e-137, Method: Compositional matrix adjust. Identities = 251/475 (52%), Positives = 347/475 (73%), Gaps = 2/475 (0%) Query: 2 IDLNNIRRGPIMLALIIGMFVAALNETLMGNALPALMQSFDASAATVQWLSTAYMLVVGV 61 +DL+NI+ PI++ALI+G VA LNETL+GNAL LM+ FD +A+T+QWLSTAYMLVVGV Sbjct: 13 VDLSNIKHTPILIALILGAMVAFLNETLLGNALTVLMKEFDVTASTIQWLSTAYMLVVGV 72 Query: 62 LVPITAILQQWFTTRQLFLSAMGLFFIGTVISAVSPVFSVLLIGRIIQAVGTGMLLPLVM 121 LVPITA+LQQW TTRQ+FL+AM F +GT+I+ +P FSVLLIGRI+QA+GTG++ PL+M Sbjct: 73 LVPITALLQQWLTTRQMFLTAMLTFLVGTLIAGFAPTFSVLLIGRIVQAIGTGLISPLLM 132 Query: 122 NVIMTIYPPEKRXXXXXXXXXXXXFAPAIGPTISGLIVDGLSWRWLFYLVIPFALISIFV 181 N I+ I PPEKR F+PA+GPT+SGLIVD L+WRWLFY VIP +ISI + Sbjct: 133 NTILIICPPEKRGATMGLIGLVMMFSPAVGPTLSGLIVDSLNWRWLFYTVIPIVIISILI 192 Query: 182 GAAVLKNVSEVTKPRIDFLSIVLSTIGFGGIVYGFSSAGEHGWAVPEVIWTIAVGGVALL 241 G ++NV+E+T+P++D+LSI+LST+GFGGIVY FS++G+ GW+ P+V +++ +G ++L Sbjct: 193 GMKYIQNVTELTRPKVDYLSILLSTLGFGGIVYSFSASGDLGWSDPKVYYSLMIGAISLF 252 Query: 242 LFVWRQLKLEQPIMDLRAFRYPXXXXXXXXXXXXXXXXXSSAIMLPMFMQGVLLITAFKS 301 +FV RQLK++ PI++LRAF++P +S +LPMF+Q VLL+TAFKS Sbjct: 253 IFVVRQLKIDNPILELRAFKFPMFTLTVVLMFIIMMSLFASMTLLPMFLQTVLLVTAFKS 312 Query: 302 GLILLPGGIVNGVMAPISGKLFDKFGPRILIIPGLTIAVISLWQFTRFNEATSTGFIVSV 361 GLI+LPG IV+G+M P++GKLFDK P+ +IIPG+ + +++W F N T+ I+ + Sbjct: 313 GLIMLPGSIVSGIMGPVAGKLFDKLSPKTIIIPGIGLVAVAMWLFKGLNPETTLTQIILM 372 Query: 362 HIALMIGIALVMMPAQTTGLNQLPRNLHPHGTAILNTLQQVAGAIGTALYISIMSGGQKQ 421 H LM+G+ V M AQT GLNQL L+PHGTA+ NTL QVAGAIGTA++IS MS G Q Sbjct: 373 HSLLMVGLMFV-MTAQTYGLNQLTPELYPHGTALFNTLSQVAGAIGTAIFISKMSSGTTQ 431 Query: 422 YLSGVSDPSASSEITKGLAAGINNAFWFGVIVALIALGIGLFLR-KGKSPEHEQL 475 Y+ ++P S+E L +G AF G+I+ ++AL + FL+ + K + QL Sbjct: 432 YMENSANPMDSAEKLNSLTSGFQGAFTLGLILVVVALVVSFFLKGQKKKAKATQL 486 >C2V806.1 C2V806_BACCE Lincomycin resistance protein Length = 491 Score = 486 bits (1250), Expect = e-135, Method: Compositional matrix adjust. Identities = 247/468 (52%), Positives = 338/468 (72%), Gaps = 1/468 (0%) Query: 2 IDLNNIRRGPIMLALIIGMFVAALNETLMGNALPALMQSFDASAATVQWLSTAYMLVVGV 61 +D +NI+ PI++AL++G VA LNETL+GNAL LM+ FD +A+T+QWLSTAYMLVVGV Sbjct: 13 VDTSNIKHTPILIALLLGAMVALLNETLLGNALTVLMKEFDVTASTIQWLSTAYMLVVGV 72 Query: 62 LVPITAILQQWFTTRQLFLSAMGLFFIGTVISAVSPVFSVLLIGRIIQAVGTGMLLPLVM 121 LVPITA+LQQW TTRQ+FL AM F +GT+I+ +P FSVLL+GRI+QAV TG++ PL+M Sbjct: 73 LVPITALLQQWLTTRQMFLIAMVTFLVGTLIAGFAPTFSVLLVGRIVQAVATGLISPLLM 132 Query: 122 NVIMTIYPPEKRXXXXXXXXXXXXFAPAIGPTISGLIVDGLSWRWLFYLVIPFALISIFV 181 N I+ I PPEKR APAIGPT+SG+IVD L+WRWLFY+VIP +ISI + Sbjct: 133 NTILIICPPEKRGATMGLIALVMMSAPAIGPTLSGVIVDSLNWRWLFYIVIPIVIISIMI 192 Query: 182 GAAVLKNVSEVTKPRIDFLSIVLSTIGFGGIVYGFSSAGEHGWAVPEVIWTIAVGGVALL 241 G ++NVSE+T+P++D+ SI+LST+GFGG+VY FS++G+ GW+ +V T+ VG ++L Sbjct: 193 GMKYIQNVSELTRPKVDYPSILLSTLGFGGLVYSFSASGDLGWSDAKVYGTLLVGLISLF 252 Query: 242 LFVWRQLKLEQPIMDLRAFRYPXXXXXXXXXXXXXXXXXSSAIMLPMFMQGVLLITAFKS 301 +FV RQLK+E PI++LRAF+ P S+ +LPMF+Q VLL+TAFKS Sbjct: 253 IFVVRQLKIENPILELRAFKVPMFTLSVGLIVIVMMSLFSTMTLLPMFLQTVLLVTAFKS 312 Query: 302 GLILLPGGIVNGVMAPISGKLFDKFGPRILIIPGLTIAVISLWQFTRFNEATSTGFIVSV 361 G+I+LPG I++ +M PI+GKLFDKF P+++I+PG+ + I+++ F TS I+ + Sbjct: 313 GIIMLPGSIISAIMGPIAGKLFDKFSPKVIIVPGIVLVGIAMFLFKDITPDTSMLQIIVM 372 Query: 362 HIALMIGIALVMMPAQTTGLNQLPRNLHPHGTAILNTLQQVAGAIGTALYISIMSGGQKQ 421 H LM+G+ V M AQT GLNQL +L+PHGTA+ NTLQQVAGAIGTA++IS MS G Q Sbjct: 373 HSVLMVGLMFV-MTAQTYGLNQLTPDLYPHGTALFNTLQQVAGAIGTAIFISKMSSGTTQ 431 Query: 422 YLSGVSDPSASSEITKGLAAGINNAFWFGVIVALIALGIGLFLRKGKS 469 Y+ V++P E GL +G AF G+I ++A + LFL++ K Sbjct: 432 YMESVANPMDPVEKLNGLTSGFQGAFALGLIFIVVAFIVSLFLKEEKK 479 >C2PBD6.1 C2PBD6_BACCE Lincomycin resistance protein lmrB Length = 483 Score = 474 bits (1219), Expect = e-132, Method: Compositional matrix adjust. Identities = 241/471 (51%), Positives = 341/471 (72%), Gaps = 1/471 (0%) Query: 3 DLNNIRRGPIMLALIIGMFVAALNETLMGNALPALMQSFDASAATVQWLSTAYMLVVGVL 62 + +N++R PI++++IIG F LNETL+ A P LM + + +T+QWLST YMLVV VL Sbjct: 14 EFDNVKRLPILISMIIGAFFTILNETLLNVAFPQLMIELNVTPSTLQWLSTGYMLVVAVL 73 Query: 63 VPITAILQQWFTTRQLFLSAMGLFFIGTVISAVSPVFSVLLIGRIIQAVGTGMLLPLVMN 122 +P +A+L QWFTTRQ+F+ AM +F GT +SA++P FS+LL+GR++QA GTG+++P++MN Sbjct: 74 IPASALLVQWFTTRQVFIGAMVVFTFGTFVSAIAPGFSILLVGRLLQAAGTGLMMPVLMN 133 Query: 123 VIMTIYPPEKRXXXXXXXXXXXXFAPAIGPTISGLIVDGLSWRWLFYLVIPFALISIFVG 182 I+ +YPPEKR FAPAIGPT+SG+I++ LSWRWLFY+V+PFA+ SI Sbjct: 134 TILLLYPPEKRGAAMGSIGLVIMFAPAIGPTLSGIILETLSWRWLFYIVLPFAIFSIVFA 193 Query: 183 AAVLKNVSEVTKPRIDFLSIVLSTIGFGGIVYGFSSAGEHGWAVPEVIWTIAVGGVALLL 242 LKNVS+ TKP++D LSI+LSTIGFGGIVYGFSS+GE GW +V I +G VALL Sbjct: 194 FIYLKNVSQPTKPKVDVLSILLSTIGFGGIVYGFSSSGE-GWDSFQVYGIILIGLVALLF 252 Query: 243 FVWRQLKLEQPIMDLRAFRYPXXXXXXXXXXXXXXXXXSSAIMLPMFMQGVLLITAFKSG 302 FV RQLKL++P++DL AF+YP S+ +LP QG L +T + +G Sbjct: 253 FVLRQLKLKEPLLDLSAFKYPMFTLTTILLTIMMMTMFSTMTLLPFLFQGALGLTVYATG 312 Query: 303 LILLPGGIVNGVMAPISGKLFDKFGPRILIIPGLTIAVISLWQFTRFNEATSTGFIVSVH 362 LI+LPG ++NG+++P+SGKLFDKFGPR LIIPG + +W FT+ TS + +H Sbjct: 313 LIMLPGSLLNGLLSPVSGKLFDKFGPRALIIPGTILLAGVMWFFTQVTTDTSKITFILLH 372 Query: 363 IALMIGIALVMMPAQTTGLNQLPRNLHPHGTAILNTLQQVAGAIGTALYISIMSGGQKQY 422 + +M+ I+++MMPAQT GLNQLPR +PHGTAILNTL QVAGAIG A +IS+M+ GQK+Y Sbjct: 373 VTMMVSISMIMMPAQTNGLNQLPRRFYPHGTAILNTLSQVAGAIGVAFFISVMTSGQKKY 432 Query: 423 LSGVSDPSASSEITKGLAAGINNAFWFGVIVALIALGIGLFLRKGKSPEHE 473 L S+P+ +++T+ + +G++NAF G +AL+A+ + LF+++ ++ E+E Sbjct: 433 LDASSNPTDPAQLTEAMVSGVHNAFTIGFGLALLAVVVALFIKRSQTSENE 483 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.327 0.142 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,550,387,314 Number of extensions: 99496191 Number of successful extensions: 528134 Number of sequences better than 1.0e-05: 9516 Number of HSP's gapped: 510400 Number of HSP's successfully gapped: 9532 Length of query: 477 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 337 Effective length of database: 1,741,754,103 Effective search space: 586971132711 Effective search space used: 586971132711 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 133 (55.8 bits)