BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0386.0031.p (459 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1IIQ9.1 B1IIQ9_CLOBK MATE efflux family protein 476 e-132 A7FSZ8.1 A7FSZ8_CLOB1 MATE efflux family protein 476 e-132 A5I0U5.1 A5I0U5_CLOBH MATE efflux family protein 476 e-132 B1QKJ3.1 B1QKJ3_CLOBO MATE efflux family protein 476 e-132 C1FK56.1 C1FK56_CLOBJ MATE efflux family protein 475 e-132 >B1IIQ9.1 B1IIQ9_CLOBK MATE efflux family protein Length = 455 Score = 476 bits (1225), Expect = e-132, Method: Compositional matrix adjust. Identities = 245/449 (54%), Positives = 343/449 (76%), Gaps = 3/449 (0%) Query: 6 EQLGSEPIRKLLLKFSIPAIAGMLVSSLYNLLDRIFVGRL---GALAMTGIGLTLPFMTL 62 +QLG E ++KLLLKFS PAI GM+V++LYN++DR+++G + G+LA+TG+GLTLP MT+ Sbjct: 5 KQLGEESVKKLLLKFSAPAIMGMIVNALYNIIDRMYIGHIKDVGSLAITGVGLTLPIMTV 64 Query: 63 LTAFTSLVGIGTSAIVSMRLGQNRKEDAEKVLGNAFSMLTVLMLVVTAIGLIFKEPILTL 122 L AF+ L+GIG+++I+S+RLGQ RK DAEK+LGNAF++L ++M+ +T IGLIF +P+L + Sbjct: 65 LMAFSMLIGIGSASIISIRLGQKRKNDAEKILGNAFTLLCIIMISITIIGLIFVDPLLNI 124 Query: 123 LGASELTMGYASDYITIILIGSVFQGIGAGLSNVIRAEGHPAKATIVLVIGTVLDMILNP 182 GAS T YA +Y+ IILIGS+ +G GL+N IRAEG+P A + +++G VL++IL+P Sbjct: 125 FGASAKTFYYAKEYVVIILIGSITNALGFGLNNSIRAEGNPKMAMVTMLLGAVLNLILDP 184 Query: 183 LFIFTFNMGIAGAAWTTVLSATVTTILVIAHFVGKKSVLKLRIVNLKLSLPIVKDILSIG 242 +FIF+FNMGI GAA TV+S T TI V+ +F GKKS LKL+ N K+ I +I+SIG Sbjct: 185 IFIFSFNMGIKGAAIATVISQTANTIWVLRYFTGKKSTLKLKRENFKVEKTIFLEIISIG 244 Query: 243 LSPFFMQISAFIVGIISNNALKTYGGDVAIGAMTVVNAVMILFLMSAMGITQGAQPILGF 302 ++PF +Q++A I+ +ISNNALK GGD+AIGAMTV+N+V ++ LM GI QGAQPI+G+ Sbjct: 245 MAPFALQLAASIITVISNNALKNTGGDLAIGAMTVINSVSLMVLMPIFGINQGAQPIIGY 304 Query: 303 NYGAQQYDRVKKTLKLELALVTAICVLTFLMVQIFPTGLARILSNDLELINAASHGMKIF 362 NYGA+QY RVK TLK+ + T + + FL+VQIFP + +I +ND +L+ G++I Sbjct: 305 NYGAEQYKRVKDTLKIAIFSATMVVTVGFLLVQIFPQYIIKIFNNDPKLMEIGISGLRIV 364 Query: 363 LLMLPIIGAQIVGASYFQAVGAAKKAMFLGLLRQVITLIPMLIILPNFFGLTGVWAAAPT 422 L MLP+IG QIV ++YFQA+G AK ++FL LLRQVI LIP+L+ILPN FGLTGVW AP Sbjct: 365 LCMLPVIGFQIVSSNYFQAIGKAKVSVFLSLLRQVIILIPLLLILPNHFGLTGVWMCAPI 424 Query: 423 SDFISCSVAIVFVAREIKRLGKAEQHQAE 451 SD IS V +F+ REI +LG+ + ++ + Sbjct: 425 SDGISSIVTAIFIYREINKLGREKSYKVK 453 >A7FSZ8.1 A7FSZ8_CLOB1 MATE efflux family protein Length = 455 Score = 476 bits (1224), Expect = e-132, Method: Compositional matrix adjust. Identities = 245/449 (54%), Positives = 343/449 (76%), Gaps = 3/449 (0%) Query: 6 EQLGSEPIRKLLLKFSIPAIAGMLVSSLYNLLDRIFVGRL---GALAMTGIGLTLPFMTL 62 +QLG E ++KLLLKFS PAI GM+V++LYN++DR+++G + G+LA+TG+GLTLP MT+ Sbjct: 5 KQLGEESVKKLLLKFSAPAIMGMIVNALYNIIDRMYIGHIKDVGSLAITGVGLTLPIMTV 64 Query: 63 LTAFTSLVGIGTSAIVSMRLGQNRKEDAEKVLGNAFSMLTVLMLVVTAIGLIFKEPILTL 122 L AF+ L+GIG+++I+S+RLGQ RK+DAEK+LGNAF++L ++M+ +T IGLIF +P+L + Sbjct: 65 LMAFSMLIGIGSASIISIRLGQKRKKDAEKILGNAFTLLCIIMISITIIGLIFVDPLLNI 124 Query: 123 LGASELTMGYASDYITIILIGSVFQGIGAGLSNVIRAEGHPAKATIVLVIGTVLDMILNP 182 GAS T YA +Y+ IILIGS+ +G GL+N IRAEG+P A + +++G VL++IL+P Sbjct: 125 FGASAKTFYYAKEYVVIILIGSITNALGFGLNNSIRAEGNPKMAMVTMLLGAVLNLILDP 184 Query: 183 LFIFTFNMGIAGAAWTTVLSATVTTILVIAHFVGKKSVLKLRIVNLKLSLPIVKDILSIG 242 +FIF FNMGI GAA TV+S T TI V+ +F GKKS LKL+ N K+ I +I+SIG Sbjct: 185 IFIFGFNMGIKGAAIATVISQTANTIWVLRYFTGKKSTLKLKRENFKVEKTIFLEIISIG 244 Query: 243 LSPFFMQISAFIVGIISNNALKTYGGDVAIGAMTVVNAVMILFLMSAMGITQGAQPILGF 302 ++PF +Q++A I+ +ISNNALK GGD+AIGAMTV+N+V ++ LM GI QGAQPI+G+ Sbjct: 245 MAPFALQLAASIITVISNNALKNTGGDLAIGAMTVINSVSLMVLMPIFGINQGAQPIIGY 304 Query: 303 NYGAQQYDRVKKTLKLELALVTAICVLTFLMVQIFPTGLARILSNDLELINAASHGMKIF 362 NYGA+QY RVK TLK+ + T + + FL+VQIFP + +I +ND +L+ G++I Sbjct: 305 NYGAEQYKRVKDTLKIAIFSATMVVTVGFLLVQIFPQYIIKIFNNDPKLMEIGISGLRIV 364 Query: 363 LLMLPIIGAQIVGASYFQAVGAAKKAMFLGLLRQVITLIPMLIILPNFFGLTGVWAAAPT 422 L MLP+IG QIV ++YFQA+G AK ++FL LLRQVI LIP+L+ILPN FGLTGVW AP Sbjct: 365 LCMLPVIGFQIVSSNYFQAIGKAKVSVFLSLLRQVIILIPLLLILPNHFGLTGVWMCAPI 424 Query: 423 SDFISCSVAIVFVAREIKRLGKAEQHQAE 451 SD IS V +F+ REI +LG+ + ++ + Sbjct: 425 SDGISSIVTAIFIYREINKLGREKSYKVK 453 >A5I0U5.1 A5I0U5_CLOBH MATE efflux family protein Length = 455 Score = 476 bits (1224), Expect = e-132, Method: Compositional matrix adjust. Identities = 245/449 (54%), Positives = 343/449 (76%), Gaps = 3/449 (0%) Query: 6 EQLGSEPIRKLLLKFSIPAIAGMLVSSLYNLLDRIFVGRL---GALAMTGIGLTLPFMTL 62 +QLG E ++KLLLKFS PAI GM+V++LYN++DR+++G + G+LA+TG+GLTLP MT+ Sbjct: 5 KQLGEESVKKLLLKFSAPAIMGMIVNALYNIIDRMYIGHIKDVGSLAITGVGLTLPIMTV 64 Query: 63 LTAFTSLVGIGTSAIVSMRLGQNRKEDAEKVLGNAFSMLTVLMLVVTAIGLIFKEPILTL 122 L AF+ L+GIG+++I+S+RLGQ RK+DAEK+LGNAF++L ++M+ +T IGLIF +P+L + Sbjct: 65 LMAFSMLIGIGSASIISIRLGQKRKKDAEKILGNAFTLLCIIMISITIIGLIFVDPLLNI 124 Query: 123 LGASELTMGYASDYITIILIGSVFQGIGAGLSNVIRAEGHPAKATIVLVIGTVLDMILNP 182 GAS T YA +Y+ IILIGS+ +G GL+N IRAEG+P A + +++G VL++IL+P Sbjct: 125 FGASAKTFYYAKEYVVIILIGSITNALGFGLNNSIRAEGNPKMAMVTMLLGAVLNLILDP 184 Query: 183 LFIFTFNMGIAGAAWTTVLSATVTTILVIAHFVGKKSVLKLRIVNLKLSLPIVKDILSIG 242 +FIF FNMGI GAA TV+S T TI V+ +F GKKS LKL+ N K+ I +I+SIG Sbjct: 185 IFIFGFNMGIKGAAIATVISQTANTIWVLRYFTGKKSTLKLKRENFKVEKTIFLEIISIG 244 Query: 243 LSPFFMQISAFIVGIISNNALKTYGGDVAIGAMTVVNAVMILFLMSAMGITQGAQPILGF 302 ++PF +Q++A I+ +ISNNALK GGD+AIGAMTV+N+V ++ LM GI QGAQPI+G+ Sbjct: 245 MAPFALQLAASIITVISNNALKNTGGDLAIGAMTVINSVSLMVLMPIFGINQGAQPIIGY 304 Query: 303 NYGAQQYDRVKKTLKLELALVTAICVLTFLMVQIFPTGLARILSNDLELINAASHGMKIF 362 NYGA+QY RVK TLK+ + T + + FL+VQIFP + +I +ND +L+ G++I Sbjct: 305 NYGAEQYKRVKDTLKIAIFSATMVVTVGFLLVQIFPQYIIKIFNNDPKLMEIGISGLRIV 364 Query: 363 LLMLPIIGAQIVGASYFQAVGAAKKAMFLGLLRQVITLIPMLIILPNFFGLTGVWAAAPT 422 L MLP+IG QIV ++YFQA+G AK ++FL LLRQVI LIP+L+ILPN FGLTGVW AP Sbjct: 365 LCMLPVIGFQIVSSNYFQAIGKAKVSVFLSLLRQVIILIPLLLILPNHFGLTGVWMCAPI 424 Query: 423 SDFISCSVAIVFVAREIKRLGKAEQHQAE 451 SD IS V +F+ REI +LG+ + ++ + Sbjct: 425 SDGISSIVTAIFIYREINKLGREKSYKVK 453 >B1QKJ3.1 B1QKJ3_CLOBO MATE efflux family protein Length = 455 Score = 476 bits (1224), Expect = e-132, Method: Compositional matrix adjust. Identities = 246/450 (54%), Positives = 343/450 (76%), Gaps = 3/450 (0%) Query: 5 NEQLGSEPIRKLLLKFSIPAIAGMLVSSLYNLLDRIFVGRL---GALAMTGIGLTLPFMT 61 +QLG E ++KLLLKFS PAI GM+V++LYN++DR+++G + G+LA+TG+GLTLP MT Sbjct: 4 QKQLGEESVKKLLLKFSAPAIMGMIVNALYNIIDRMYIGHIKDVGSLAITGVGLTLPIMT 63 Query: 62 LLTAFTSLVGIGTSAIVSMRLGQNRKEDAEKVLGNAFSMLTVLMLVVTAIGLIFKEPILT 121 +L AF+ L+GIG+++I+S+RLGQ RK DAEK+LGNAF++L ++M+ +T IGLIF +P+L Sbjct: 64 VLMAFSMLIGIGSASIISIRLGQQRKNDAEKILGNAFTLLCIIMISITIIGLIFVDPLLN 123 Query: 122 LLGASELTMGYASDYITIILIGSVFQGIGAGLSNVIRAEGHPAKATIVLVIGTVLDMILN 181 + GASE T YA +YI IILIGS+ +G GL+N+IRAEG+P A + +++G VL++IL+ Sbjct: 124 IFGASEQTFYYAKEYIVIILIGSITNALGFGLNNLIRAEGNPKMAMVTMLLGAVLNLILD 183 Query: 182 PLFIFTFNMGIAGAAWTTVLSATVTTILVIAHFVGKKSVLKLRIVNLKLSLPIVKDILSI 241 P+FIF FNMGI GAA TV+S T TTI V+ +F GKKS LKL+ N K+ I +I+SI Sbjct: 184 PIFIFGFNMGIKGAAIATVISQTATTIWVLRYFTGKKSTLKLKRENFKVEKTIFLEIISI 243 Query: 242 GLSPFFMQISAFIVGIISNNALKTYGGDVAIGAMTVVNAVMILFLMSAMGITQGAQPILG 301 G++PF +Q++A I+ +ISNNALK GGD+AIGAMTV+N+V ++ LM GI QGAQPI+G Sbjct: 244 GMAPFALQLAASIITVISNNALKNTGGDLAIGAMTVINSVSLMVLMPIFGINQGAQPIIG 303 Query: 302 FNYGAQQYDRVKKTLKLELALVTAICVLTFLMVQIFPTGLARILSNDLELINAASHGMKI 361 +NYGA+QY RVK TLK+ + T + + F +VQIFP + +I +ND +L+ G++I Sbjct: 304 YNYGAEQYKRVKDTLKIAIFSATIVVTVGFSLVQIFPQYIIKIFNNDPKLMEIGISGLRI 363 Query: 362 FLLMLPIIGAQIVGASYFQAVGAAKKAMFLGLLRQVITLIPMLIILPNFFGLTGVWAAAP 421 L MLP+IG QIV ++YFQA+G AK ++FL LLRQVI LIP+L+ILP FGLTGVW AP Sbjct: 364 VLCMLPVIGFQIVSSNYFQAIGKAKVSVFLSLLRQVIILIPLLLILPKHFGLTGVWMCAP 423 Query: 422 TSDFISCSVAIVFVAREIKRLGKAEQHQAE 451 SD IS V +F+ REI +LG+ + ++ + Sbjct: 424 ISDGISSIVTAIFIYREINKLGREKSYKVK 453 >C1FK56.1 C1FK56_CLOBJ MATE efflux family protein Length = 455 Score = 475 bits (1223), Expect = e-132, Method: Compositional matrix adjust. Identities = 245/449 (54%), Positives = 341/449 (75%), Gaps = 3/449 (0%) Query: 6 EQLGSEPIRKLLLKFSIPAIAGMLVSSLYNLLDRIFVGRL---GALAMTGIGLTLPFMTL 62 +QLG E ++KLLLKFS PAI GM+V++LYN++DR+++G + G+LA+TG+GLTLP MT+ Sbjct: 5 KQLGEESVKKLLLKFSAPAIMGMIVNALYNIIDRMYIGHIKDVGSLAITGVGLTLPIMTV 64 Query: 63 LTAFTSLVGIGTSAIVSMRLGQNRKEDAEKVLGNAFSMLTVLMLVVTAIGLIFKEPILTL 122 L AF+ L+GIG+++I+S+RLGQ RK DAEK+LGNAF++L ++M+ +T IGLIF +P+L + Sbjct: 65 LMAFSMLIGIGSASIISIRLGQKRKNDAEKILGNAFTLLCIIMISITIIGLIFVDPLLNI 124 Query: 123 LGASELTMGYASDYITIILIGSVFQGIGAGLSNVIRAEGHPAKATIVLVIGTVLDMILNP 182 GAS T YA +Y+ IILIGS+ +G GL+N IRAEG+P A + +++G VL++IL+P Sbjct: 125 FGASAKTFYYAKEYVVIILIGSITNALGFGLNNSIRAEGNPKMAMVTMLLGAVLNLILDP 184 Query: 183 LFIFTFNMGIAGAAWTTVLSATVTTILVIAHFVGKKSVLKLRIVNLKLSLPIVKDILSIG 242 +FIF FNMGI GAA TV+S T TI V+ +F GKKS LKL+ N K+ I +I+SIG Sbjct: 185 IFIFGFNMGIKGAAIATVISQTANTIWVLRYFTGKKSTLKLKRENFKVEKTIFLEIISIG 244 Query: 243 LSPFFMQISAFIVGIISNNALKTYGGDVAIGAMTVVNAVMILFLMSAMGITQGAQPILGF 302 ++PF +Q++A I+ +ISNNALK GGD+AIGAMTV+N+V ++ LM GI QGAQPI+G+ Sbjct: 245 MAPFALQLAASIITVISNNALKNTGGDLAIGAMTVINSVSLMVLMPIFGINQGAQPIIGY 304 Query: 303 NYGAQQYDRVKKTLKLELALVTAICVLTFLMVQIFPTGLARILSNDLELINAASHGMKIF 362 NYGA+QY RVK TLK+ + T + + FL+VQIFP + +I +ND +L+ G++I Sbjct: 305 NYGAEQYKRVKDTLKIAIFSATMVVTVGFLLVQIFPQYIIKIFNNDPKLMEIGISGLRIV 364 Query: 363 LLMLPIIGAQIVGASYFQAVGAAKKAMFLGLLRQVITLIPMLIILPNFFGLTGVWAAAPT 422 L MLP+IG QIV ++YFQA+G AK ++FL LLRQVI LIP+L ILPN FGLTGVW AP Sbjct: 365 LCMLPVIGFQIVSSNYFQAIGKAKVSVFLSLLRQVIILIPLLFILPNHFGLTGVWMCAPI 424 Query: 423 SDFISCSVAIVFVAREIKRLGKAEQHQAE 451 SD IS V +F+ REI +LG+ + ++ + Sbjct: 425 SDGISSIVTAIFIYREINKLGREKSYKVK 453 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.327 0.141 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,378,475,137 Number of extensions: 94908389 Number of successful extensions: 415563 Number of sequences better than 1.0e-05: 4088 Number of HSP's gapped: 407175 Number of HSP's successfully gapped: 4260 Length of query: 459 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 320 Effective length of database: 1,751,175,118 Effective search space: 560376037760 Effective search space used: 560376037760 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 133 (55.8 bits)